ID NEU1_HUMAN Reviewed; 125 AA. AC P01178; Q3MIG0; DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot. DT 01-JAN-1988, sequence version 1. DT 13-FEB-2019, entry version 171. DE RecName: Full=Oxytocin-neurophysin 1; DE Short=OT-NPI; DE Contains: DE RecName: Full=Oxytocin; DE AltName: Full=Ocytocin; DE Contains: DE RecName: Full=Neurophysin 1; DE Flags: Precursor; GN Name=OXT; Synonyms=OT; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=3768139; RA Rehbein M., Hillers M., Mohr E., Ivell R., Morley S., Schmale H., RA Richter D.; RT "The neurohypophyseal hormones vasopressin and oxytocin. Precursor RT structure, synthesis and regulation."; RL Biol. Chem. Hoppe-Seyler 367:695-704(1986). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=2991279; RA Sausville E., Carney D., Battey J.; RT "The human vasopressin gene is linked to the oxytocin gene and is RT selectively expressed in a cultured lung cancer cell line."; RL J. Biol. Chem. 260:10236-10241(1985). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RA Grunwald W.C. Jr., Cool D.R.; RT "Lack of sequence polymorphism in the oxytocin gene of autistic RT patients."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=11780052; DOI=10.1038/414865a; RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., RA Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., RA Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., RA Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., RA Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., RA Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., RA Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., RA Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., RA Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., RA Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., RA Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., RA Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., RA Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., RA Rogers J.; RT "The DNA sequence and comparative analysis of human chromosome 20."; RL Nature 414:865-871(2001). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Lung; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [6] RP NUCLEOTIDE SEQUENCE [MRNA] OF 25-110. RX PubMed=2249637; DOI=10.1210/endo-127-6-2990; RA Ivell R., Furuya K., Brackmann B., Dawood Y., Khan-Dawood F.; RT "Expression of the oxytocin and vasopressin genes in human and baboon RT gonadal tissues."; RL Endocrinology 127:2990-2996(1990). RN [7] RP NUCLEOTIDE SEQUENCE [MRNA] OF 80-125. RX PubMed=4065330; DOI=10.1016/0014-5793(85)80069-7; RA Mohr E., Hillers M., Ivell R., Haulica I.D., Richter D.; RT "Expression of the vasopressin and oxytocin genes in human RT hypothalami."; RL FEBS Lett. 193:12-16(1985). RN [8] RP PROTEIN SEQUENCE OF 32-125. RX PubMed=6574452; DOI=10.1073/pnas.80.10.2839; RA Chauvet M.-T., Hurpet D., Chauvet J., Acher R.; RT "Identification of human neurophysins: complete amino acid sequences RT of MSEL- and VLDV-neurophysins."; RL Proc. Natl. Acad. Sci. U.S.A. 80:2839-2843(1983). RN [9] RP PROTEIN SEQUENCE OF 32-125. RX PubMed=7262323; DOI=10.1016/0014-5793(81)80109-3; RA Schlesinger D.H., Audhya T.K.; RT "A comparative study of mammalian neurophysin protein sequences."; RL FEBS Lett. 128:325-328(1981). RN [10] RP PROTEIN SEQUENCE OF 20-28, AND AMIDATION AT GLY-28. RX PubMed=13591312; RA Light A., du Vigneaud V.; RT "On the nature of oxytocin and vasopressin from human pituitary."; RL Proc. Soc. Exp. Biol. Med. 98:692-696(1958). RN [11] RP FUNCTION OF OXYTOCIN, MUTAGENESIS OF GLY-28, AND SUBUNIT. RX PubMed=18174156; DOI=10.1074/jbc.M706477200; RA Dutertre S., Croker D., Daly N.L., Andersson A., Muttenthaler M., RA Lumsden N.G., Craik D.J., Alewood P.F., Guillon G., Lewis R.J.; RT "Conopressin-T from Conus tulipa reveals an antagonist switch in RT vasopressin-like peptides."; RL J. Biol. Chem. 283:7100-7108(2008). CC -!- FUNCTION: Neurophysin 1 specifically binds oxytocin. CC -!- FUNCTION: Oxytocin causes contraction of the smooth muscle of the CC uterus and of the mammary gland. Acts by binding to oxytocin CC receptor (OXTR) (PubMed:18174156). {ECO:0000269|PubMed:18174156}. CC -!- SUBUNIT: Interacts with oxytocin receptor (Ki=1.5 nM) CC (PubMed:18174156). Interacts with vasopressin V1aR/AVPR1A (Ki=37 CC nM), V1bR/AVPR1B (Ki=222 nM) and V2R/AVPR2 receptors (Ki=823 nM) CC (PubMed:18174156). {ECO:0000269|PubMed:18174156}. CC -!- SUBCELLULAR LOCATION: Secreted. CC -!- PHARMACEUTICAL: Oxytocin is available under the names Pitocin CC (Parke-Davis) and Syntocinon (Sandoz). Used to artificially speed CC or induce labor. CC -!- SIMILARITY: Belongs to the vasopressin/oxytocin family. CC {ECO:0000305}. CC -!- WEB RESOURCE: Name=Wikipedia; Note=Oxytocin entry; CC URL="https://en.wikipedia.org/wiki/Oxytocin"; CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC ----------------------------------------------------------------------- DR EMBL; M25650; AAA59977.1; -; mRNA. DR EMBL; M11186; AAA98806.1; -; Genomic_DNA. DR EMBL; AY082910; AAL92860.1; -; Genomic_DNA. DR EMBL; AL160414; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; BC069144; AAH69144.1; -; mRNA. DR EMBL; BC101841; AAI01842.1; -; mRNA. DR EMBL; BC101843; AAI01844.1; -; mRNA. DR EMBL; X03173; CAA26936.1; -; mRNA. DR EMBL; M62611; AAA59979.1; -; mRNA. DR CCDS; CCDS13044.1; -. DR PIR; A94676; NFHU1. DR RefSeq; NP_000906.1; NM_000915.3. DR RefSeq; XP_011527540.1; XM_011529238.1. DR UniGene; Hs.113216; -. DR ProteinModelPortal; P01178; -. DR SMR; P01178; -. DR BioGrid; 111060; 7. DR IntAct; P01178; 1. DR STRING; 9606.ENSP00000217386; -. DR DrugBank; DB00107; Oxytocin. DR BioMuta; OXT; -. DR DMDM; 128071; -. DR PaxDb; P01178; -. DR PeptideAtlas; P01178; -. DR PRIDE; P01178; -. DR ProteomicsDB; 51341; -. DR Ensembl; ENST00000217386; ENSP00000217386; ENSG00000101405. DR GeneID; 5020; -. DR KEGG; hsa:5020; -. DR CTD; 5020; -. DR DisGeNET; 5020; -. DR EuPathDB; HostDB:ENSG00000101405.3; -. DR GeneCards; OXT; -. DR HGNC; HGNC:8528; OXT. DR HPA; HPA071892; -. DR MIM; 167050; gene. DR neXtProt; NX_P01178; -. DR OpenTargets; ENSG00000101405; -. DR PharmGKB; PA32857; -. DR eggNOG; ENOG410J3A8; Eukaryota. DR eggNOG; ENOG41124QF; LUCA. DR GeneTree; ENSGT00390000004511; -. DR HOGENOM; HOG000113768; -. DR HOVERGEN; HBG004462; -. DR InParanoid; P01178; -. DR KO; K05243; -. DR OMA; GLCCDAE; -. DR OrthoDB; 1548839at2759; -. DR PhylomeDB; P01178; -. DR TreeFam; TF333018; -. DR Reactome; R-HSA-388479; Vasopressin-like receptors. DR Reactome; R-HSA-416476; G alpha (q) signalling events. DR SIGNOR; P01178; -. DR GeneWiki; Neurophysin_I; -. DR GeneWiki; Oxytocin; -. DR GenomeRNAi; 5020; -. DR PMAP-CutDB; P01178; -. DR PRO; PR:P01178; -. DR Proteomes; UP000005640; Chromosome 20. DR Bgee; ENSG00000101405; Expressed in 79 organ(s), highest expression level in hypothalamus. DR Genevisible; P01178; HS. DR GO; GO:0005576; C:extracellular region; TAS:Reactome. DR GO; GO:0005615; C:extracellular space; IEA:Ensembl. DR GO; GO:0030141; C:secretory granule; IEA:Ensembl. DR GO; GO:0043195; C:terminal bouton; IEA:Ensembl. DR GO; GO:0005185; F:neurohypophyseal hormone activity; IEA:InterPro. DR GO; GO:0031855; F:oxytocin receptor binding; IEA:Ensembl. DR GO; GO:0042756; P:drinking behavior; IEA:Ensembl. DR GO; GO:0042755; P:eating behavior; IEA:Ensembl. DR GO; GO:0007565; P:female pregnancy; IEA:Ensembl. DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; TAS:Reactome. DR GO; GO:0007625; P:grooming behavior; IEA:Ensembl. DR GO; GO:0007507; P:heart development; IEA:Ensembl. DR GO; GO:0042538; P:hyperosmotic salinity response; IEA:Ensembl. DR GO; GO:0060179; P:male mating behavior; IEA:Ensembl. DR GO; GO:0002125; P:maternal aggressive behavior; IEA:Ensembl. DR GO; GO:0042711; P:maternal behavior; IEA:Ensembl. DR GO; GO:0007613; P:memory; IEA:Ensembl. DR GO; GO:0045776; P:negative regulation of blood pressure; IEA:Ensembl. DR GO; GO:0060455; P:negative regulation of gastric acid secretion; IEA:Ensembl. DR GO; GO:0035811; P:negative regulation of urine volume; IEA:Ensembl. DR GO; GO:0045777; P:positive regulation of blood pressure; IEA:Ensembl. DR GO; GO:0120162; P:positive regulation of cold-induced thermogenesis; ISS:YuBioLab. DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IEA:Ensembl. DR GO; GO:0045925; P:positive regulation of female receptivity; IEA:Ensembl. DR GO; GO:0060450; P:positive regulation of hindgut contraction; IEA:Ensembl. DR GO; GO:0010701; P:positive regulation of norepinephrine secretion; IEA:Ensembl. DR GO; GO:0045778; P:positive regulation of ossification; IEA:Ensembl. DR GO; GO:0060406; P:positive regulation of penile erection; IEA:Ensembl. DR GO; GO:0032308; P:positive regulation of prostaglandin secretion; IEA:Ensembl. DR GO; GO:0035815; P:positive regulation of renal sodium excretion; IEA:Ensembl. DR GO; GO:0051965; P:positive regulation of synapse assembly; IEA:Ensembl. DR GO; GO:0050806; P:positive regulation of synaptic transmission; IEA:Ensembl. DR GO; GO:0070474; P:positive regulation of uterine smooth muscle contraction; IEA:Ensembl. DR GO; GO:0002027; P:regulation of heart rate; IEA:Ensembl. DR GO; GO:0051930; P:regulation of sensory perception of pain; IEA:Ensembl. DR GO; GO:0014823; P:response to activity; IEA:Ensembl. DR GO; GO:0001975; P:response to amphetamine; IEA:Ensembl. DR GO; GO:0051591; P:response to cAMP; IEA:Ensembl. DR GO; GO:0042220; P:response to cocaine; IEA:Ensembl. DR GO; GO:0051602; P:response to electrical stimulus; IEA:Ensembl. DR GO; GO:0032355; P:response to estradiol; IEA:Ensembl. DR GO; GO:0045472; P:response to ether; IEA:Ensembl. DR GO; GO:0032094; P:response to food; IEA:Ensembl. DR GO; GO:0051384; P:response to glucocorticoid; IEA:Ensembl. DR GO; GO:0043434; P:response to peptide hormone; IEA:Ensembl. DR GO; GO:0032570; P:response to progesterone; IEA:Ensembl. DR GO; GO:0034695; P:response to prostaglandin E; IEA:Ensembl. DR GO; GO:0032526; P:response to retinoic acid; IEA:Ensembl. DR GO; GO:0009744; P:response to sucrose; IEA:Ensembl. DR GO; GO:0007165; P:signal transduction; TAS:ProtInc. DR GO; GO:0030431; P:sleep; IEA:Ensembl. DR GO; GO:0035176; P:social behavior; IEA:Ensembl. DR GO; GO:0042713; P:sperm ejaculation; IEA:Ensembl. DR Gene3D; 2.60.9.10; -; 1. DR InterPro; IPR000981; Neurhyp_horm. DR InterPro; IPR036387; Neurhyp_horm_dom_sf. DR InterPro; IPR022423; Neurohypophysial_hormone_CS. DR PANTHER; PTHR11681; PTHR11681; 1. DR Pfam; PF00220; Hormone_4; 1. DR Pfam; PF00184; Hormone_5; 1. DR PIRSF; PIRSF001815; Nonapeptide_hormone_precursor; 1. DR PRINTS; PR00831; NEUROPHYSIN. DR ProDom; PD001676; Neurhyp_horm; 1. DR SMART; SM00003; NH; 1. DR SUPFAM; SSF49606; SSF49606; 1. DR PROSITE; PS00264; NEUROHYPOPHYS_HORM; 1. PE 1: Evidence at protein level; KW Amidation; Cleavage on pair of basic residues; Complete proteome; KW Direct protein sequencing; Disulfide bond; Hormone; Pharmaceutical; KW Reference proteome; Secreted; Signal. FT SIGNAL 1 19 {ECO:0000269|PubMed:13591312}. FT PEPTIDE 20 28 Oxytocin. {ECO:0000269|PubMed:13591312}. FT /FTId=PRO_0000020495. FT CHAIN 32 125 Neurophysin 1. FT {ECO:0000269|PubMed:6574452, FT ECO:0000269|PubMed:7262323}. FT /FTId=PRO_0000020496. FT MOD_RES 28 28 Glycine amide. FT {ECO:0000269|PubMed:13591312}. FT DISULFID 20 25 FT DISULFID 41 85 {ECO:0000250|UniProtKB:P01175}. FT DISULFID 44 58 {ECO:0000250|UniProtKB:P01175}. FT DISULFID 52 75 {ECO:0000250|UniProtKB:P01175}. FT DISULFID 59 65 {ECO:0000250|UniProtKB:P01175}. FT DISULFID 92 104 {ECO:0000250|UniProtKB:P01175}. FT DISULFID 98 116 {ECO:0000250|UniProtKB:P01175}. FT DISULFID 105 110 {ECO:0000250|UniProtKB:P01175}. FT MUTAGEN 28 28 G->V: Gain of antagonist activity on FT V1aR/AVPR1A (and loss of agonist activity FT on this receptor). 310-fold decrease in FT affinity for oxytocin receptor (OXTR), FT 13-fold decrease in affinity for FT V1aR/AVPR1A, and complete loss of FT affinity for V1bR/AVPR1B and V2R/AVPR2. FT {ECO:0000269|PubMed:18174156}. FT CONFLICT 94 94 S -> G (in Ref. 6; AAA59979). FT {ECO:0000305}. FT CONFLICT 100 101 VL -> AA (in Ref. 9; AA sequence). FT {ECO:0000305}. FT CONFLICT 100 100 Missing (in Ref. 2; AAA98806). FT {ECO:0000305}. FT CONFLICT 124 124 Q -> L (in Ref. 9; AA sequence). FT {ECO:0000305}. SQ SEQUENCE 125 AA; 12722 MW; C65BB544731A0E7C CRC64; MAGPSLACCL LGLLALTSAC YIQNCPLGGK RAAPDLDVRK CLPCGPGGKG RCFGPNICCA EELGCFVGTA EALRCQEENY LPSPCQSGQK ACGSGGRCAV LGLCCSPDGC HADPACDAEA TFSQR //