ID   CST8_HUMAN              Reviewed;         142 AA.
AC   O60676; Q2M2X6;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   13-FEB-2019, entry version 150.
DE   RecName: Full=Cystatin-8;
DE   AltName: Full=Cystatin-related epididymal spermatogenic protein;
DE   Flags: Precursor;
GN   Name=CST8; Synonyms=CRES;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Testis;
RX   PubMed=7619504; DOI=10.1002/mrd.1080410107;
RA   Cornwall G.A., Hann S.R.;
RT   "Transient appearance of CRES protein during spermatogenesis and caput
RT   epididymal sperm maturation.";
RL   Mol. Reprod. Dev. 41:37-46(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11780052; DOI=10.1038/414865a;
RA   Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA   Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA   Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M.,
RA   Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J.,
RA   Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P.,
RA   Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M.,
RA   Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R.,
RA   Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M.,
RA   Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA   Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA   Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA   Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA   Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA   Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA   Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA   Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA   Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA   Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H.,
RA   Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S.,
RA   Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E.,
RA   Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A.,
RA   Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M.,
RA   Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A.,
RA   Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S.,
RA   Rogers J.;
RT   "The DNA sequence and comparative analysis of human chromosome 20.";
RL   Nature 414:865-871(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-39.
RC   TISSUE=Saliva;
RX   PubMed=16740002; DOI=10.1021/pr050492k;
RA   Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T.,
RA   Loo J.A.;
RT   "Identification of N-linked glycoproteins in human saliva by
RT   glycoprotein capture and mass spectrometry.";
RL   J. Proteome Res. 5:1493-1503(2006).
CC   -!- FUNCTION: Performs a specialized role during sperm development and
CC       maturation.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Proximal caput region of the epididymis. Lower
CC       expression in the testis. Within the testis it is localized to the
CC       elongating spermatids, whereas within the epididymis it is
CC       exclusively synthesized by the proximal caput epithelium.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   EMBL; AF059244; AAC14707.1; -; mRNA.
DR   EMBL; AL109954; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC069496; AAH69496.1; -; mRNA.
DR   EMBL; BC105113; AAI05114.1; -; mRNA.
DR   EMBL; BC105119; AAI05120.1; -; mRNA.
DR   CCDS; CCDS13156.1; -.
DR   RefSeq; NP_001268659.1; NM_001281730.1.
DR   RefSeq; NP_005483.1; NM_005492.3.
DR   UniGene; Hs.121602; -.
DR   ProteinModelPortal; O60676; -.
DR   SMR; O60676; -.
DR   BioGrid; 115358; 35.
DR   STRING; 9606.ENSP00000246012; -.
DR   MEROPS; I25.027; -.
DR   iPTMnet; O60676; -.
DR   PhosphoSitePlus; O60676; -.
DR   BioMuta; CST8; -.
DR   PaxDb; O60676; -.
DR   PeptideAtlas; O60676; -.
DR   PRIDE; O60676; -.
DR   ProteomicsDB; 49521; -.
DR   DNASU; 10047; -.
DR   Ensembl; ENST00000246012; ENSP00000246012; ENSG00000125815.
DR   GeneID; 10047; -.
DR   KEGG; hsa:10047; -.
DR   UCSC; uc002wth.3; human.
DR   CTD; 10047; -.
DR   DisGeNET; 10047; -.
DR   EuPathDB; HostDB:ENSG00000125815.8; -.
DR   GeneCards; CST8; -.
DR   HGNC; HGNC:2480; CST8.
DR   MIM; 608683; gene.
DR   neXtProt; NX_O60676; -.
DR   OpenTargets; ENSG00000125815; -.
DR   PharmGKB; PA26981; -.
DR   eggNOG; ENOG410IXH4; Eukaryota.
DR   eggNOG; ENOG41113F6; LUCA.
DR   GeneTree; ENSGT00940000162294; -.
DR   HOGENOM; HOG000231754; -.
DR   HOVERGEN; HBG095685; -.
DR   InParanoid; O60676; -.
DR   KO; K13904; -.
DR   OMA; KQCLWFA; -.
DR   OrthoDB; 1565344at2759; -.
DR   PhylomeDB; O60676; -.
DR   GeneWiki; CST8_(gene); -.
DR   GenomeRNAi; 10047; -.
DR   PRO; PR:O60676; -.
DR   Proteomes; UP000005640; Chromosome 20.
DR   Bgee; ENSG00000125815; Expressed in 12 organ(s), highest expression level in right testis.
DR   ExpressionAtlas; O60676; baseline and differential.
DR   Genevisible; O60676; HS.
DR   GO; GO:0009986; C:cell surface; IEA:Ensembl.
DR   GO; GO:0005737; C:cytoplasm; IEA:Ensembl.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; TAS:ProtInc.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR027214; Cystatin.
DR   InterPro; IPR000010; Cystatin_dom.
DR   PANTHER; PTHR11413; PTHR11413; 1.
DR   Pfam; PF00031; Cystatin; 1.
DR   SMART; SM00043; CY; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Disulfide bond; Glycoprotein; Polymorphism;
KW   Protease inhibitor; Reference proteome; Secreted; Signal;
KW   Thiol protease inhibitor.
FT   SIGNAL        1     21       {ECO:0000255}.
FT   CHAIN        22    142       Cystatin-8.
FT                                /FTId=PRO_0000006653.
FT   MOTIF        77     81       Secondary area of contact. {ECO:0000255}.
FT   CARBOHYD     27     27       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD     39     39       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000269|PubMed:16740002}.
FT   DISULFID     95    105       {ECO:0000250}.
FT   DISULFID    119    139       {ECO:0000250}.
FT   VARIANT      52     52       A -> V (in dbSNP:rs35190670).
FT                                /FTId=VAR_061130.
FT   VARIANT     142    142       A -> P (in dbSNP:rs1054633).
FT                                /FTId=VAR_014527.
SQ   SEQUENCE   142 AA;  16275 MW;  9A3512757E0F4ECD CRC64;
     MPRCRWLSLI LLTIPLALVA RKDPKKNETG VLRKLKPVNA SNANVKQCLW FAMQEYNKES
     EDKYVFLVVK TLQAQLQVTN LLEYLIDVEI ARSDCRKPLS TNEICAIQEN SKLKRKLSCS
     FLVGALPWNG EFTVMEKKCE DA
//