ID   ISK4_HUMAN              Reviewed;          86 AA.
AC   O60575; Q2YDT7;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   13-FEB-2019, entry version 130.
DE   RecName: Full=Serine protease inhibitor Kazal-type 4;
DE   AltName: Full=Peptide PEC-60 homolog;
DE   Flags: Precursor;
GN   Name=SPINK4;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Hu G.;
RT   "Human gastrointestinal peptide mRNA, homolog of swine PEC-60.";
RL   Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15164053; DOI=10.1038/nature02465;
RA   Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E.,
RA   Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C.,
RA   Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S.,
RA   Babbage A.K., Babbage S., Bagguley C.L., Bailey J., Banerjee R.,
RA   Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P.,
RA   Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W.,
RA   Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G.,
RA   Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M.,
RA   Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W.,
RA   Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A.,
RA   Frankland J.A., French L., Fricker D.G., Garner P., Garnett J.,
RA   Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA   Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA   Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA   Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA   Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA   Kimberley A.M., King A., Knights A., Laird G.K., Langford C.,
RA   Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M.,
RA   Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S.,
RA   McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J.,
RA   Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R.,
RA   Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M.,
RA   Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M.,
RA   Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A.,
RA   Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P.,
RA   Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W.,
RA   Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA   Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S.,
RA   Rogers J., Dunham I.;
RT   "DNA sequence and analysis of human chromosome 9.";
RL   Nature 429:369-374(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
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DR   EMBL; AF048700; AAC05124.1; -; mRNA.
DR   EMBL; AL161445; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC110068; AAI10069.1; -; mRNA.
DR   CCDS; CCDS6536.1; -.
DR   RefSeq; NP_055286.1; NM_014471.2.
DR   UniGene; Hs.555934; -.
DR   ProteinModelPortal; O60575; -.
DR   SMR; O60575; -.
DR   BioGrid; 118114; 9.
DR   IntAct; O60575; 1.
DR   MEROPS; I01.970; -.
DR   BioMuta; SPINK4; -.
DR   jPOST; O60575; -.
DR   PaxDb; O60575; -.
DR   PeptideAtlas; O60575; -.
DR   PRIDE; O60575; -.
DR   ProteomicsDB; 49476; -.
DR   Ensembl; ENST00000379721; ENSP00000369045; ENSG00000122711.
DR   GeneID; 27290; -.
DR   KEGG; hsa:27290; -.
DR   CTD; 27290; -.
DR   DisGeNET; 27290; -.
DR   EuPathDB; HostDB:ENSG00000122711.8; -.
DR   GeneCards; SPINK4; -.
DR   HGNC; HGNC:16646; SPINK4.
DR   HPA; HPA007286; -.
DR   MIM; 613929; gene.
DR   neXtProt; NX_O60575; -.
DR   OpenTargets; ENSG00000122711; -.
DR   PharmGKB; PA38180; -.
DR   eggNOG; KOG3649; Eukaryota.
DR   eggNOG; ENOG410YC3T; LUCA.
DR   GeneTree; ENSGT00530000064269; -.
DR   HOGENOM; HOG000090244; -.
DR   HOVERGEN; HBG006182; -.
DR   InParanoid; O60575; -.
DR   OMA; PRMPICE; -.
DR   OrthoDB; 1588023at2759; -.
DR   PhylomeDB; O60575; -.
DR   ChiTaRS; SPINK4; human.
DR   GenomeRNAi; 27290; -.
DR   PRO; PR:O60575; -.
DR   Proteomes; UP000005640; Chromosome 9.
DR   Bgee; ENSG00000122711; Expressed in 77 organ(s), highest expression level in small intestine Peyer's patch.
DR   ExpressionAtlas; O60575; baseline and differential.
DR   Genevisible; O60575; HS.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0042493; P:response to drug; IEA:Ensembl.
DR   InterPro; IPR002350; Kazal_dom.
DR   InterPro; IPR036058; Kazal_dom_sf.
DR   InterPro; IPR039932; Spink4-like.
DR   PANTHER; PTHR21179; PTHR21179; 1.
DR   Pfam; PF00050; Kazal_1; 1.
DR   SMART; SM00280; KAZAL; 1.
DR   SUPFAM; SSF100895; SSF100895; 1.
DR   PROSITE; PS00282; KAZAL_1; 1.
DR   PROSITE; PS51465; KAZAL_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Disulfide bond; Polymorphism; Protease inhibitor;
KW   Reference proteome; Secreted; Serine protease inhibitor; Signal.
FT   SIGNAL        1     26       {ECO:0000250}.
FT   CHAIN        27     86       Serine protease inhibitor Kazal-type 4.
FT                                /FTId=PRO_0000016569.
FT   DOMAIN       31     86       Kazal-like. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00798}.
FT   SITE         48     49       Reactive bond. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00798}.
FT   DISULFID     37     68       {ECO:0000255|PROSITE-ProRule:PRU00798}.
FT   DISULFID     46     65       {ECO:0000255|PROSITE-ProRule:PRU00798}.
FT   DISULFID     54     86       {ECO:0000255|PROSITE-ProRule:PRU00798}.
FT   VARIANT       7      7       V -> I (in dbSNP:rs706107).
FT                                /FTId=VAR_011898.
SQ   SEQUENCE   86 AA;  9454 MW;  374CD4B3CAC9AF52 CRC64;
     MAVRQWVIAL ALAALLVVDR EVPVAAGKLP FSRMPICEHM VESPTCSQMS NLVCGTDGLT
     YTNECQLCLA RIKTKQDIQI MKDGKC
//