ID   SEM3E_HUMAN             Reviewed;         775 AA.
AC   O15041; B4E1P1; Q75M94; Q75M97;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   13-FEB-2019, entry version 170.
DE   RecName: Full=Semaphorin-3E;
DE   Flags: Precursor;
GN   Name=SEMA3E; Synonyms=KIAA0331, SEMAH;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Brain;
RX   PubMed=9205841; DOI=10.1093/dnares/4.2.141;
RA   Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N.,
RA   Tanaka A., Kotani H., Nomura N., Ohara O.;
RT   "Prediction of the coding sequences of unidentified human genes. VII.
RT   The complete sequences of 100 new cDNA clones from brain which can
RT   code for large proteins in vitro.";
RL   DNA Res. 4:141-150(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Trachea;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12853948; DOI=10.1038/nature01782;
RA   Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA   Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R.,
RA   Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E.,
RA   Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H.,
RA   Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A.,
RA   Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J.,
RA   Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A.,
RA   Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S.,
RA   Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M.,
RA   Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C.,
RA   Latreille P., Miller N., Johnson D., Murray J., Woessner J.P.,
RA   Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J.,
RA   Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L.,
RA   Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R.,
RA   Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA   Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K.,
RA   Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S.,
RA   Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M.,
RA   Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R.,
RA   Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D.,
RA   Waterston R.H., Wilson R.K.;
RT   "The DNA sequence of human chromosome 7.";
RL   Nature 424:157-164(2003).
RN   [4]
RP   VARIANTS PRO-208; CYS-619; LEU-703 AND VAL-717.
RX   PubMed=15235037;
RA   Lalani S.R., Safiullah A.M., Molinari L.M., Fernbach S.D.,
RA   Martin D.M., Belmont J.W.;
RT   "SEMA3E mutation in a patient with CHARGE syndrome.";
RL   J. Med. Genet. 41:E94-E94(2004).
CC   -!- FUNCTION: Plays an important role in signaling via the cell
CC       surface receptor PLXND1. Mediates reorganization of the actin
CC       cytoskeleton, leading to the retraction of cell projections.
CC       Promotes focal adhesion disassembly and inhibits adhesion of
CC       endothelial cells to the extracellular matrix. Regulates
CC       angiogenesis, both during embryogenesis and after birth. Can down-
CC       regulate sprouting angiogenesis. Required for normal vascular
CC       patterning during embryogenesis. Plays an important role in
CC       ensuring the specificity of synapse formation (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with PLXND1. {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q9Y4D7:PLXND1; NbExp=2; IntAct=EBI-7283693, EBI-310731;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=O15041-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=O15041-2; Sequence=VSP_046237;
CC         Note=No experimental confirmation available.;
CC   -!- SIMILARITY: Belongs to the semaphorin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA20789.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AB002329; BAA20789.2; ALT_INIT; mRNA.
DR   EMBL; AK303925; BAG64853.1; -; mRNA.
DR   EMBL; AC004954; AAC69513.1; -; Genomic_DNA.
DR   EMBL; AC006204; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC079799; AAS07488.1; -; Genomic_DNA.
DR   EMBL; AC079987; AAS07487.1; -; Genomic_DNA.
DR   CCDS; CCDS34674.1; -. [O15041-1]
DR   CCDS; CCDS55121.1; -. [O15041-2]
DR   RefSeq; NP_001171600.1; NM_001178129.1. [O15041-2]
DR   RefSeq; NP_036563.1; NM_012431.2. [O15041-1]
DR   UniGene; Hs.440730; -.
DR   UniGene; Hs.528721; -.
DR   ProteinModelPortal; O15041; -.
DR   SMR; O15041; -.
DR   BioGrid; 115072; 1.
DR   IntAct; O15041; 1.
DR   MINT; O15041; -.
DR   STRING; 9606.ENSP00000303212; -.
DR   iPTMnet; O15041; -.
DR   PhosphoSitePlus; O15041; -.
DR   BioMuta; SEMA3E; -.
DR   jPOST; O15041; -.
DR   PaxDb; O15041; -.
DR   PeptideAtlas; O15041; -.
DR   PRIDE; O15041; -.
DR   ProteomicsDB; 48396; -.
DR   Ensembl; ENST00000307792; ENSP00000303212; ENSG00000170381. [O15041-1]
DR   Ensembl; ENST00000427262; ENSP00000405052; ENSG00000170381. [O15041-2]
DR   Ensembl; ENST00000643230; ENSP00000496491; ENSG00000170381. [O15041-1]
DR   GeneID; 9723; -.
DR   KEGG; hsa:9723; -.
DR   UCSC; uc003uhy.3; human. [O15041-1]
DR   CTD; 9723; -.
DR   DisGeNET; 9723; -.
DR   EuPathDB; HostDB:ENSG00000170381.12; -.
DR   GeneCards; SEMA3E; -.
DR   GeneReviews; SEMA3E; -.
DR   HGNC; HGNC:10727; SEMA3E.
DR   HPA; HPA029419; -.
DR   HPA; HPA063804; -.
DR   MalaCards; SEMA3E; -.
DR   MIM; 608166; gene.
DR   neXtProt; NX_O15041; -.
DR   OpenTargets; ENSG00000170381; -.
DR   Orphanet; 138; CHARGE syndrome.
DR   PharmGKB; PA35649; -.
DR   eggNOG; KOG3611; Eukaryota.
DR   eggNOG; ENOG410XQZC; LUCA.
DR   GeneTree; ENSGT00940000158437; -.
DR   HOGENOM; HOG000039964; -.
DR   HOVERGEN; HBG055071; -.
DR   InParanoid; O15041; -.
DR   KO; K06840; -.
DR   OMA; YWGRDAA; -.
DR   OrthoDB; 158418at2759; -.
DR   PhylomeDB; O15041; -.
DR   TreeFam; TF352628; -.
DR   Reactome; R-HSA-416700; Other semaphorin interactions.
DR   ChiTaRS; SEMA3E; human.
DR   GenomeRNAi; 9723; -.
DR   PRO; PR:O15041; -.
DR   Proteomes; UP000005640; Chromosome 7.
DR   Bgee; ENSG00000170381; Expressed in 144 organ(s), highest expression level in lung.
DR   ExpressionAtlas; O15041; baseline and differential.
DR   Genevisible; O15041; HS.
DR   GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0045499; F:chemorepellent activity; IBA:GO_Central.
DR   GO; GO:0038191; F:neuropilin binding; IBA:GO_Central.
DR   GO; GO:0030215; F:semaphorin receptor binding; IBA:GO_Central.
DR   GO; GO:0001569; P:branching involved in blood vessel morphogenesis; ISS:UniProtKB.
DR   GO; GO:0050919; P:negative chemotaxis; IBA:GO_Central.
DR   GO; GO:0016525; P:negative regulation of angiogenesis; ISS:UniProtKB.
DR   GO; GO:0048843; P:negative regulation of axon extension involved in axon guidance; IBA:GO_Central.
DR   GO; GO:0001953; P:negative regulation of cell-matrix adhesion; ISS:UniProtKB.
DR   GO; GO:0001755; P:neural crest cell migration; IBA:GO_Central.
DR   GO; GO:0030335; P:positive regulation of cell migration; IBA:GO_Central.
DR   GO; GO:2000249; P:regulation of actin cytoskeleton reorganization; ISS:UniProtKB.
DR   GO; GO:0008360; P:regulation of cell shape; ISS:UniProtKB.
DR   GO; GO:0071526; P:semaphorin-plexin signaling pathway; ISS:UniProtKB.
DR   GO; GO:0002040; P:sprouting angiogenesis; ISS:UniProtKB.
DR   GO; GO:0050808; P:synapse organization; ISS:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 1.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR013151; Immunoglobulin.
DR   InterPro; IPR016201; PSI.
DR   InterPro; IPR001627; Semap_dom.
DR   InterPro; IPR036352; Semap_dom_sf.
DR   InterPro; IPR027231; Semaphorin.
DR   InterPro; IPR015513; Semaphorin_3E.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   PANTHER; PTHR11036; PTHR11036; 1.
DR   PANTHER; PTHR11036:SF22; PTHR11036:SF22; 1.
DR   Pfam; PF00047; ig; 1.
DR   Pfam; PF01403; Sema; 1.
DR   SMART; SM00423; PSI; 1.
DR   SMART; SM00630; Sema; 1.
DR   SUPFAM; SSF101912; SSF101912; 1.
DR   SUPFAM; SSF48726; SSF48726; 1.
DR   PROSITE; PS50835; IG_LIKE; 1.
DR   PROSITE; PS51004; SEMA; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Angiogenesis; Complete proteome;
KW   Developmental protein; Differentiation; Disulfide bond; Glycoprotein;
KW   Immunoglobulin domain; Neurogenesis; Polymorphism; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL        1     25       {ECO:0000255}.
FT   CHAIN        26    775       Semaphorin-3E.
FT                                /FTId=PRO_0000032317.
FT   DOMAIN       32    516       Sema. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00352}.
FT   DOMAIN      581    669       Ig-like C2-type.
FT   COMPBIAS    737    770       Arg/Lys-rich (basic).
FT   CARBOHYD     44     44       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    126    126       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    330    330       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    595    595       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    596    596       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   DISULFID    105    115       {ECO:0000250}.
FT   DISULFID    133    142       {ECO:0000250}.
FT   DISULFID    270    382       {ECO:0000250}.
FT   DISULFID    294    342       {ECO:0000250}.
FT   DISULFID    519    537       {ECO:0000250}.
FT   DISULFID    654    729       {ECO:0000250}.
FT   VAR_SEQ       1     60       Missing (in isoform 2).
FT                                {ECO:0000303|PubMed:14702039}.
FT                                /FTId=VSP_046237.
FT   VARIANT     208    208       R -> P (in dbSNP:rs61729612).
FT                                {ECO:0000269|PubMed:15235037}.
FT                                /FTId=VAR_080476.
FT   VARIANT     619    619       R -> C (in dbSNP:rs143631464).
FT                                {ECO:0000269|PubMed:15235037}.
FT                                /FTId=VAR_080477.
FT   VARIANT     703    703       S -> L (found in a patient with CHARGE
FT                                syndrome; unknown pathological
FT                                significance; dbSNP:rs121918341).
FT                                {ECO:0000269|PubMed:15235037}.
FT                                /FTId=VAR_080478.
FT   VARIANT     717    717       I -> V (in dbSNP:rs61729610).
FT                                {ECO:0000269|PubMed:15235037}.
FT                                /FTId=VAR_080479.
SQ   SEQUENCE   775 AA;  89228 MW;  CD6079C1AE48F779 CRC64;
     MASAGHIITL LLWGYLLELW TGGHTADTTH PRLRLSHKEL LNLNRTSIFH SPFGFLDLHT
     MLLDEYQERL FVGGRDLVYS LSLERISDGY KEIHWPSTAL KMEECIMKGK DAGECANYVR
     VLHHYNRTHL LTCGTGAFDP VCAFIRVGYH LEDPLFHLES PRSERGRGRC PFDPSSSFIS
     TLIGSELFAG LYSDYWSRDA AIFRSMGRLA HIRTEHDDER LLKEPKFVGS YMIPDNEDRD
     DNKVYFFFTE KALEAENNAH AIYTRVGRLC VNDVGGQRIL VNKWSTFLKA RLVCSVPGMN
     GIDTYFDELE DVFLLPTRDH KNPVIFGLFN TTSNIFRGHA ICVYHMSSIR AAFNGPYAHK
     EGPEYHWSVY EGKVPYPRPG SCASKVNGGR YGTTKDYPDD AIRFARSHPL MYQAIKPAHK
     KPILVKTDGK YNLKQIAVDR VEAEDGQYDV LFIGTDNGIV LKVITIYNQE MESMEEVILE
     ELQIFKDPVP IISMEISSKR QQLYIGSASA VAQVRFHHCD MYGSACADCC LARDPYCAWD
     GISCSRYYPT GTHAKRRFRR QDVRHGNAAQ QCFGQQFVGD ALDKTEEHLA YGIENNSTLL
     ECTPRSLQAK VIWFVQKGRE TRKEEVKTDD RVVKMDLGLL FLRLHKSDAG TYFCQTVEHS
     FVHTVRKITL EVVEEEKVED MFNKDDEEDR HHRMPCPAQS SISQGAKPWY KEFLQLIGYS
     NFQRVEEYCE KVWCTDRKRK KLKMSPSKWK YANPQEKKLR SKPEHYRLPR HTLDS
//