ID   NEMP1_HUMAN             Reviewed;         444 AA.
AC   O14524; Q17R72; Q68DH0; Q6IQ25;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 2.
DT   13-FEB-2019, entry version 140.
DE   RecName: Full=Nuclear envelope integral membrane protein 1;
DE   Flags: Precursor;
GN   Name=NEMP1; Synonyms=KIAA0286, TMEM194, TMEM194A;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Brain;
RX   PubMed=9179496; DOI=10.1093/dnares/4.1.53;
RA   Ohara O., Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N.,
RA   Nomura N.;
RT   "Construction and characterization of human brain cDNA libraries
RT   suitable for analysis of cDNA clones encoding relatively large
RT   proteins.";
RL   DNA Res. 4:53-59(1997).
RN   [2]
RP   SEQUENCE REVISION.
RX   PubMed=12168954; DOI=10.1093/dnares/9.3.99;
RA   Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.;
RT   "Construction of expression-ready cDNA clones for KIAA genes: manual
RT   curation of 330 KIAA cDNA clones.";
RL   DNA Res. 9:99-106(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Amygdala;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Endometrial tumor;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H.,
RA   Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K.,
RA   Ottenwaelder B., Poustka A., Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-368, AND IDENTIFICATION
RP   BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA   Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA   Greff Z., Keri G., Stemmann O., Mann M.;
RT   "Kinase-selective enrichment enables quantitative phosphoproteomics of
RT   the kinome across the cell cycle.";
RL   Mol. Cell 31:438-448(2008).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-368, AND IDENTIFICATION
RP   BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA   Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA   Elledge S.J., Gygi S.P.;
RT   "A quantitative atlas of mitotic phosphorylation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-424 AND SER-425, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D.,
RA   Wang L., Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human
RT   liver phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
CC   -!- SUBUNIT: Homooligomer. Interacts with RAN-GTP.
CC       {ECO:0000250|UniProtKB:B9X187, ECO:0000250|UniProtKB:Q6ZQE4}.
CC   -!- SUBCELLULAR LOCATION: Nucleus inner membrane
CC       {ECO:0000250|UniProtKB:Q6ZQE4}; Multi-pass membrane protein
CC       {ECO:0000255}; Nucleoplasmic side {ECO:0000250|UniProtKB:B9X187}.
CC       Nucleus envelope {ECO:0000250|UniProtKB:Q6ZQE4}. Note=Colocalizes
CC       with lamins and RAN-GTP at the nuclear envelope.
CC       {ECO:0000250|UniProtKB:Q6ZQE4}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=O14524-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=O14524-2; Sequence=VSP_011896;
CC         Note=No experimental confirmation available.;
CC   -!- DOMAIN: The transmembrane domains are required and sufficient for
CC       its oligomerization. {ECO:0000250|UniProtKB:B9X187}.
CC   -!- PTM: Phosphorylation may regulate its interaction with RAN-GTP.
CC       {ECO:0000250|UniProtKB:Q6ZQE4}.
CC   -!- SIMILARITY: Belongs to the NEMP family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH71597.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
CC       Sequence=BAA22955.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
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DR   EMBL; AB006624; BAA22955.2; ALT_INIT; mRNA.
DR   EMBL; CR749406; CAH18250.1; -; mRNA.
DR   EMBL; AK294566; BAG57761.1; -; mRNA.
DR   EMBL; BC071597; AAH71597.1; ALT_INIT; mRNA.
DR   EMBL; BC117436; AAI17437.1; -; mRNA.
DR   EMBL; BC126300; AAI26301.1; -; mRNA.
DR   CCDS; CCDS31841.1; -. [O14524-2]
DR   CCDS; CCDS44927.1; -. [O14524-1]
DR   RefSeq; NP_001124435.1; NM_001130963.1. [O14524-1]
DR   RefSeq; NP_056072.2; NM_015257.2. [O14524-2]
DR   UniGene; Hs.591040; -.
DR   ProteinModelPortal; O14524; -.
DR   BioGrid; 116898; 31.
DR   IntAct; O14524; 29.
DR   MINT; O14524; -.
DR   STRING; 9606.ENSP00000300128; -.
DR   GlyConnect; 1577; -.
DR   iPTMnet; O14524; -.
DR   PhosphoSitePlus; O14524; -.
DR   BioMuta; NEMP1; -.
DR   EPD; O14524; -.
DR   jPOST; O14524; -.
DR   MaxQB; O14524; -.
DR   PaxDb; O14524; -.
DR   PeptideAtlas; O14524; -.
DR   PRIDE; O14524; -.
DR   ProteomicsDB; 48069; -.
DR   ProteomicsDB; 48070; -. [O14524-2]
DR   DNASU; 23306; -.
DR   Ensembl; ENST00000300128; ENSP00000300128; ENSG00000166881. [O14524-1]
DR   Ensembl; ENST00000379391; ENSP00000368701; ENSG00000166881. [O14524-2]
DR   GeneID; 23306; -.
DR   KEGG; hsa:23306; -.
DR   UCSC; uc001smx.4; human. [O14524-1]
DR   CTD; 23306; -.
DR   EuPathDB; HostDB:ENSG00000166881.9; -.
DR   GeneCards; NEMP1; -.
DR   H-InvDB; HIX0010743; -.
DR   HGNC; HGNC:29001; NEMP1.
DR   HPA; HPA014394; -.
DR   MIM; 616496; gene.
DR   neXtProt; NX_O14524; -.
DR   OpenTargets; ENSG00000166881; -.
DR   PharmGKB; PA162406290; -.
DR   eggNOG; KOG3817; Eukaryota.
DR   eggNOG; ENOG410ZZKM; LUCA.
DR   GeneTree; ENSGT00390000002174; -.
DR   HOGENOM; HOG000122573; -.
DR   HOVERGEN; HBG052180; -.
DR   InParanoid; O14524; -.
DR   OMA; INILSWT; -.
DR   OrthoDB; 536946at2759; -.
DR   PhylomeDB; O14524; -.
DR   TreeFam; TF314831; -.
DR   GenomeRNAi; 23306; -.
DR   PRO; PR:O14524; -.
DR   Proteomes; UP000005640; Chromosome 12.
DR   Bgee; ENSG00000166881; Expressed in 180 organ(s), highest expression level in female gonad.
DR   ExpressionAtlas; O14524; baseline and differential.
DR   Genevisible; O14524; HS.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005635; C:nuclear envelope; ISS:UniProtKB.
DR   GO; GO:0005637; C:nuclear inner membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR019358; NEMP_fam.
DR   PANTHER; PTHR13598; PTHR13598; 1.
DR   Pfam; PF10225; NEMP; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Complete proteome; Glycoprotein; Membrane;
KW   Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Signal;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL        1     43       {ECO:0000255}.
FT   CHAIN        44    444       Nuclear envelope integral membrane
FT                                protein 1.
FT                                /FTId=PRO_0000050744.
FT   TRANSMEM    161    181       Helical. {ECO:0000255}.
FT   TRANSMEM    186    206       Helical. {ECO:0000255}.
FT   TRANSMEM    216    236       Helical. {ECO:0000255}.
FT   TRANSMEM    245    265       Helical. {ECO:0000255}.
FT   TRANSMEM    289    309       Helical. {ECO:0000255}.
FT   REGION      186    297       A; required for its colocalization with
FT                                lamins at the nuclear envelope.
FT                                {ECO:0000250|UniProtKB:Q6ZQE4}.
FT   REGION      336    444       Required for nuclear localization.
FT                                {ECO:0000250|UniProtKB:Q6ZQE4}.
FT   REGION      336    405       B; required for interaction with RAN-GTP.
FT                                {ECO:0000250|UniProtKB:Q6ZQE4}.
FT   MOD_RES     368    368       Phosphoserine.
FT                                {ECO:0000244|PubMed:18669648,
FT                                ECO:0000244|PubMed:18691976}.
FT   MOD_RES     424    424       Phosphoserine.
FT                                {ECO:0000244|PubMed:24275569}.
FT   MOD_RES     425    425       Phosphoserine.
FT                                {ECO:0000244|PubMed:24275569}.
FT   CARBOHYD    125    125       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   VAR_SEQ     110    182       Missing (in isoform 2).
FT                                {ECO:0000303|PubMed:14702039,
FT                                ECO:0000303|PubMed:15489334,
FT                                ECO:0000303|PubMed:17974005}.
FT                                /FTId=VSP_011896.
FT   VARIANT     217    217       I -> V (in dbSNP:rs17546579).
FT                                /FTId=VAR_057817.
FT   CONFLICT    442    442       F -> L (in Ref. 4; CAH18250).
FT                                {ECO:0000305}.
SQ   SEQUENCE   444 AA;  50640 MW;  F45D4D3F9B86C77B CRC64;
     MAGGMKVAVS PAVGPGPWGS GVGGGGTVRL LLILSGCLVY GTAETDVNVV MLQESQVCEK
     RASQQFCYTN VLIPKWHDIW TRIQIRVNSS RLVRVTQVEN EEKLKELEQF SIWNFFSSFL
     KEKLNDTYVN VGLYSTKTCL KVEIIEKDTK YSVIVIRRFD PKLFLVFLLG LMLFFCGDLL
     SRSQIFYYST GMTVGIVASL LIIIFILSKF MPKKSPIYVI LVGGWSFSLY LIQLVFKNLQ
     EIWRCYWQYL LSYVLTVGFM SFAVCYKYGP LENERSINLL TWTLQLMGLC FMYSGIQIPH
     IALAIIIIAL CTKNLEHPIQ WLYITCRKVC KGAEKPVPPR LLTEEEYRIQ GEVETRKALE
     ELREFCNSPD CSAWKTVSRI QSPKRFADFV EGSSHLTPNE VSVHEQEYGL GSIIAQDEIY
     EEASSEEEDS YSRCPAITQN NFLT
//