ID   H3BP20_HUMAN            Unreviewed;       540 AA.
AC   H3BP20;
DT   18-APR-2012, integrated into UniProtKB/TrEMBL.
DT   18-APR-2012, sequence version 1.
DT   16-JAN-2019, entry version 58.
DE   RecName: Full=Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093};
DE            EC=3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093};
GN   Name=HEXA {ECO:0000313|Ensembl:ENSP00000455114};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606 {ECO:0000313|Ensembl:ENSP00000455114, ECO:0000313|Proteomes:UP000005640};
RN   [1] {ECO:0000313|Ensembl:ENSP00000455114, ECO:0000313|Proteomes:UP000005640}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16572171; DOI=10.1038/nature04601;
RA   Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA   Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA   FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA   Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA   Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA   DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R.,
RA   Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G.,
RA   Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A.,
RA   Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W.,
RA   Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X.,
RA   Sneddon K.M., Stewart S., Sougnez C., Stone S.M., Topham K.,
RA   Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S.,
RA   Nusbaum C.;
RT   "Analysis of the DNA sequence and duplication history of human
RT   chromosome 15.";
RL   Nature 440:671-675(2006).
RN   [2] {ECO:0000213|PubMed:21269460}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA   Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [3] {ECO:0000313|Ensembl:ENSP00000455114}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (FEB-2012) to UniProtKB.
RN   [4] {ECO:0000213|PubMed:24275569}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D.,
RA   Wang L., Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human
RT   liver phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
RN   [5] {ECO:0000213|PubMed:25944712}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=25944712;
RA   Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M.,
RA   Ayoub D., Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT   "N-terminome analysis of the human mitochondrial proteome.";
RL   Proteomics 15:2519-2524(2015).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine
CC         residues in N-acetyl-beta-D-hexosaminides.; EC=3.2.1.52;
CC         Evidence={ECO:0000256|PIRNR:PIRNR001093};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 20 family.
CC       {ECO:0000256|PIRNR:PIRNR001093}.
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DR   EMBL; AC009690; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; KF456090; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_001305754.1; NM_001318825.1.
DR   UniGene; Hs.604479; -.
DR   UniGene; Hs.709495; -.
DR   ProteinModelPortal; H3BP20; -.
DR   SMR; H3BP20; -.
DR   EPD; H3BP20; -.
DR   jPOST; H3BP20; -.
DR   MaxQB; H3BP20; -.
DR   PeptideAtlas; H3BP20; -.
DR   PRIDE; H3BP20; -.
DR   Ensembl; ENST00000566304; ENSP00000455114; ENSG00000213614.
DR   GeneID; 3073; -.
DR   UCSC; uc010ukn.3; human.
DR   CTD; 3073; -.
DR   EuPathDB; HostDB:ENSG00000213614.9; -.
DR   HGNC; HGNC:4878; HEXA.
DR   OpenTargets; ENSG00000213614; -.
DR   eggNOG; KOG2499; Eukaryota.
DR   eggNOG; COG3525; LUCA.
DR   GeneTree; ENSGT00390000008107; -.
DR   OMA; WHLVDDS; -.
DR   OrthoDB; 545162at2759; -.
DR   ChiTaRS; HEXA; human.
DR   GenomeRNAi; 3073; -.
DR   Proteomes; UP000005640; Chromosome 15.
DR   Bgee; ENSG00000213614; Expressed in 225 organ(s), highest expression level in tendon of biceps brachii.
DR   ExpressionAtlas; H3BP20; baseline and differential.
DR   GO; GO:0004563; F:beta-N-acetylhexosaminidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102148; F:N-acetyl-beta-D-galactosaminidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 3.30.379.10; -; 1.
DR   InterPro; IPR025705; Beta_hexosaminidase_sua/sub.
DR   InterPro; IPR015883; Glyco_hydro_20_cat.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR029018; Hex-like_dom2.
DR   InterPro; IPR029019; HEX_eukaryotic_N.
DR   Pfam; PF00728; Glyco_hydro_20; 1.
DR   Pfam; PF14845; Glycohydro_20b2; 1.
DR   PIRSF; PIRSF001093; B-hxosamndse_ab_euk_; 1.
DR   PRINTS; PR00738; GLHYDRLASE20.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF55545; SSF55545; 1.
PE   1: Evidence at protein level;
KW   Complete proteome {ECO:0000313|Proteomes:UP000005640};
KW   Disulfide bond {ECO:0000256|PIRSR:PIRSR001093-2};
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR001093};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001093};
KW   Proteomics identification {ECO:0000213|EPD:H3BP20,
KW   ECO:0000213|MaxQB:H3BP20, ECO:0000213|PeptideAtlas:H3BP20};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005640};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL        1     22       {ECO:0000256|SAM:SignalP}.
FT   CHAIN        23    540       Beta-hexosaminidase.
FT                                {ECO:0000256|SAM:SignalP}.
FT                                /FTId=PRO_5003581447.
FT   DOMAIN       23    156       Glycohydro_20b2.
FT                                {ECO:0000259|Pfam:PF14845}.
FT   DOMAIN      178    498       Glyco_hydro_20.
FT                                {ECO:0000259|Pfam:PF00728}.
FT   ACT_SITE    334    334       Proton donor.
FT                                {ECO:0000256|PIRSR:PIRSR001093-1}.
FT   DISULFID     58    115       {ECO:0000256|PIRSR:PIRSR001093-2}.
FT   DISULFID    288    339       {ECO:0000256|PIRSR:PIRSR001093-2}.
FT   DISULFID    516    533       {ECO:0000256|PIRSR:PIRSR001093-2}.
SQ   SEQUENCE   540 AA;  61999 MW;  96ED0A41744DF430 CRC64;
     MTSSRLWFSL LLAAAFAGRA TALWPWPQNF QTSDQRYVLY PNNFQFQYDV SSAAQPGCSV
     LDEAFQRYRD LLFGSGSWPR PYLTGWPHQA YPVFLGKRHT LEKNVLVVSV VTPGCNQLPT
     LESVENYTLT INDDQCLLLS ETVWGALRGL ETFSQLVWKS AEGTFFINKT EIEDFPRFPH
     RGLLLDTSRH YLPLSSILDT LDVMAYNKLN VFHWHLVDDP SFPYESFTFP ELMRKGSYNP
     VTHIYTAQDV KEVIEYARLR GIRVLAEFDT PGHTLSWGPG IPGLLTPCYS GSEPSGTFGP
     VNPSLNNTYE FMSTFFLEVS SVFPDFYLHL GGDEVDFTCW KSNPEIQDFM RKKGFGEDFK
     QLESFYIQTL LDIVSSYGKG YVVWQEVFDN KVKIQPDTII QVWREDIPVN YMKELELVTK
     AGFRALLSAP WYLNRISYGP DWKDFYIVEP LAFEGTPEQK ALVIGGEACM WGEYVDNTNL
     VPRLWPRAGA VAERLWSNKL TSDLTFAYER LSHFRCELLR RGVQAQPLNV GFCEQEFEQT
//