ID   F8WF32_HUMAN            Unreviewed;       121 AA.
AC   F8WF32;
DT   21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT   21-SEP-2011, sequence version 1.
DT   16-JAN-2019, entry version 45.
DE   RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 {ECO:0000256|RuleBase:RU361143};
GN   Name=RPN1 {ECO:0000313|Ensembl:ENSP00000419384};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606 {ECO:0000313|Ensembl:ENSP00000419384, ECO:0000313|Proteomes:UP000005640};
RN   [1] {ECO:0000313|Ensembl:ENSP00000419384, ECO:0000313|Proteomes:UP000005640}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16641997; DOI=10.1038/nature04728;
RA   Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R.,
RA   Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R.,
RA   Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V.,
RA   Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.,
RA   Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B.,
RA   Nazareth L.V., Scott G., Sodergren E., Song X.Z., Steffen D., Wei S.,
RA   Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q.,
RA   Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z.,
RA   Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C.,
RA   Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G.,
RA   Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B.,
RA   Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R.,
RA   Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J.,
RA   Liu W., Lu J., Maheshwari M., Nguyen B.V., Okwuonu G.O., Palmeiri A.,
RA   Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA   Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA   Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA   Wang J., Wang Q., Williams G.A., Wong G.K., Yao Z., Zhang J.,
RA   Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H.,
RA   Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G.,
RA   Gibbs R.A.;
RT   "The DNA sequence, annotation and analysis of human chromosome 3.";
RL   Nature 440:1194-1198(2006).
RN   [2] {ECO:0000213|PubMed:21269460}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA   Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [3] {ECO:0000313|Ensembl:ENSP00000419384}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (JUL-2011) to UniProtKB.
RN   [4] {ECO:0000213|PubMed:24275569}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D.,
RA   Wang L., Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human
RT   liver phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
RN   [5] {ECO:0000213|PubMed:25944712}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=25944712;
RA   Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M.,
RA   Ayoub D., Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT   "N-terminome analysis of the human mitochondrial proteome.";
RL   Proteomics 15:2519-2524(2015).
CC   -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex
CC       that catalyzes the initial transfer of a defined glycan
CC       (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier
CC       dolichol-pyrophosphate to an asparagine residue within an Asn-X-
CC       Ser/Thr consensus motif in nascent polypeptide chains, the first
CC       step in protein N-glycosylation. N-glycosylation occurs
CC       cotranslationally and the complex associates with the Sec61
CC       complex at the channel-forming translocon complex that mediates
CC       protein translocation across the endoplasmic reticulum (ER). All
CC       subunits are required for a maximal enzyme activity.
CC       {ECO:0000256|RuleBase:RU361143}.
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC       {ECO:0000256|RuleBase:RU361143}.
CC   -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC       {ECO:0000256|RuleBase:RU361143}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000256|RuleBase:RU361143}; Single-pass type I membrane
CC       protein {ECO:0000256|RuleBase:RU361143}.
CC   -!- SIMILARITY: Belongs to the OST1 family.
CC       {ECO:0000256|RuleBase:RU361143}.
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DR   EMBL; AC079945; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   ProteinModelPortal; F8WF32; -.
DR   jPOST; F8WF32; -.
DR   MaxQB; F8WF32; -.
DR   PeptideAtlas; F8WF32; -.
DR   PRIDE; F8WF32; -.
DR   Ensembl; ENST00000476931; ENSP00000419384; ENSG00000163902.
DR   UCSC; uc062noh.1; human.
DR   EuPathDB; HostDB:ENSG00000163902.11; -.
DR   HGNC; HGNC:10381; RPN1.
DR   OpenTargets; ENSG00000163902; -.
DR   eggNOG; KOG2291; Eukaryota.
DR   eggNOG; ENOG410XQVZ; LUCA.
DR   GeneTree; ENSGT00390000009630; -.
DR   UniPathway; UPA00378; -.
DR   ChiTaRS; RPN1; human.
DR   Proteomes; UP000005640; Chromosome 3.
DR   Bgee; ENSG00000163902; Expressed in 232 organ(s), highest expression level in corpus epididymis.
DR   ExpressionAtlas; F8WF32; baseline and differential.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:InterPro.
DR   GO; GO:0004579; F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR007676; Ribophorin_I.
DR   PANTHER; PTHR21049; PTHR21049; 1.
DR   Pfam; PF04597; Ribophorin_I; 1.
PE   1: Evidence at protein level;
KW   Complete proteome {ECO:0000313|Proteomes:UP000005640};
KW   Endoplasmic reticulum {ECO:0000256|RuleBase:RU361143};
KW   Proteomics identification {ECO:0000213|EPD:F8WF32,
KW   ECO:0000213|MaxQB:F8WF32, ECO:0000213|PeptideAtlas:F8WF32};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005640};
KW   Signal {ECO:0000256|RuleBase:RU361143}.
FT   SIGNAL        1     24       {ECO:0000256|RuleBase:RU361143}.
FT   CHAIN        25    121       Dolichyl-diphosphooligosaccharide--
FT                                protein glycosyltransferase subunit 1.
FT                                {ECO:0000256|RuleBase:RU361143}.
FT                                /FTId=PRO_5005130506.
SQ   SEQUENCE   121 AA;  12696 MW;  C999BBE92C3F49D5 CRC64;
     MEAPAAGLFL LLLLGTWAPA PGSASSEAPP LINEDVKRTV DLSSHLAKVT AEVVLAHLGG
     GSTSRATSFL LALEPELEAR LAHLGVQVKG EDEEENNLEV RETKIKGKRV CCPGSITVVP
     C
//