ID   NEMP2_HUMAN             Reviewed;         417 AA.
AC   A6NFY4; B4DYG6;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 3.
DT   13-FEB-2019, entry version 82.
DE   RecName: Full=Nuclear envelope integral membrane protein 2;
DE   Flags: Precursor;
GN   Name=NEMP2; Synonyms=TMEM194B;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H.,
RA   Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M.,
RA   Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E.,
RA   Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J.,
RA   Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C.,
RA   Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J.,
RA   Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A.,
RA   Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K.,
RA   Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M.,
RA   Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N.,
RA   Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M.,
RA   Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E.,
RA   Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P.,
RA   Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A.,
RA   Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A.,
RA   Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T.,
RA   Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D.,
RA   Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X.,
RA   McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D.,
RA   Waterston R.H., Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2
RT   and 4.";
RL   Nature 434:724-731(2005).
CC   -!- SUBCELLULAR LOCATION: Nucleus inner membrane
CC       {ECO:0000250|UniProtKB:Q6ZQE4}; Multi-pass membrane protein
CC       {ECO:0000255}; Nucleoplasmic side {ECO:0000250|UniProtKB:B9X187}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=A6NFY4-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=A6NFY4-2; Sequence=VSP_056847, VSP_056848, VSP_056849;
CC         Note=No experimental confirmation available.;
CC   -!- SIMILARITY: Belongs to the NEMP family. {ECO:0000305}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   EMBL; AK302425; BAG63728.1; -; mRNA.
DR   EMBL; AC093388; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS46476.1; -. [A6NFY4-1]
DR   RefSeq; NP_001136117.1; NM_001142645.1. [A6NFY4-1]
DR   UniGene; Hs.659824; -.
DR   ProteinModelPortal; A6NFY4; -.
DR   SMR; A6NFY4; -.
DR   BioGrid; 756056; 1.
DR   STRING; 9606.ENSP00000386292; -.
DR   iPTMnet; A6NFY4; -.
DR   PhosphoSitePlus; A6NFY4; -.
DR   BioMuta; NEMP2; -.
DR   EPD; A6NFY4; -.
DR   PaxDb; A6NFY4; -.
DR   PRIDE; A6NFY4; -.
DR   ProteomicsDB; 1089; -.
DR   Ensembl; ENST00000409150; ENSP00000386292; ENSG00000189362. [A6NFY4-1]
DR   Ensembl; ENST00000414176; ENSP00000404283; ENSG00000189362. [A6NFY4-2]
DR   GeneID; 100131211; -.
DR   KEGG; hsa:100131211; -.
DR   UCSC; uc010zgf.3; human. [A6NFY4-1]
DR   CTD; 100131211; -.
DR   EuPathDB; HostDB:ENSG00000189362.11; -.
DR   GeneCards; NEMP2; -.
DR   HGNC; HGNC:33700; NEMP2.
DR   HPA; HPA057719; -.
DR   MIM; 616497; gene.
DR   neXtProt; NX_A6NFY4; -.
DR   OpenTargets; ENSG00000189362; -.
DR   PharmGKB; PA162406311; -.
DR   eggNOG; KOG3817; Eukaryota.
DR   eggNOG; ENOG410ZZKM; LUCA.
DR   GeneTree; ENSGT00390000002174; -.
DR   HOGENOM; HOG000122573; -.
DR   HOVERGEN; HBG108538; -.
DR   InParanoid; A6NFY4; -.
DR   OMA; RYIPKYS; -.
DR   OrthoDB; 536946at2759; -.
DR   PhylomeDB; A6NFY4; -.
DR   TreeFam; TF314831; -.
DR   ChiTaRS; NEMP2; human.
DR   GenomeRNAi; 100131211; -.
DR   PRO; PR:A6NFY4; -.
DR   Proteomes; UP000005640; Chromosome 2.
DR   Bgee; ENSG00000189362; Expressed in 129 organ(s), highest expression level in tendon of biceps brachii.
DR   ExpressionAtlas; A6NFY4; baseline and differential.
DR   Genevisible; A6NFY4; HS.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005635; C:nuclear envelope; IBA:GO_Central.
DR   GO; GO:0005637; C:nuclear inner membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR019358; NEMP_fam.
DR   PANTHER; PTHR13598; PTHR13598; 1.
DR   Pfam; PF10225; NEMP; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Complete proteome; Membrane; Nucleus;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL        1     24       {ECO:0000255}.
FT   CHAIN        25    417       Nuclear envelope integral membrane
FT                                protein 2.
FT                                /FTId=PRO_0000332238.
FT   TRANSMEM    148    168       Helical. {ECO:0000255}.
FT   TRANSMEM    177    197       Helical. {ECO:0000255}.
FT   TRANSMEM    207    227       Helical. {ECO:0000255}.
FT   TRANSMEM    239    259       Helical. {ECO:0000255}.
FT   TRANSMEM    280    300       Helical. {ECO:0000255}.
FT   VAR_SEQ       1     69       Missing (in isoform 2).
FT                                {ECO:0000303|PubMed:14702039}.
FT                                /FTId=VSP_056847.
FT   VAR_SEQ     205    207       YST -> AMS (in isoform 2).
FT                                {ECO:0000303|PubMed:14702039}.
FT                                /FTId=VSP_056848.
FT   VAR_SEQ     208    417       Missing (in isoform 2).
FT                                {ECO:0000303|PubMed:14702039}.
FT                                /FTId=VSP_056849.
SQ   SEQUENCE   417 AA;  48664 MW;  51B1CAC1FEB992FF CRC64;
     MGPRQGRWWL LLWLPPLATL PVRGEAAAAA LSVRRCKALK EKDLIRTSES DCYCYNQNSQ
     VEWKYIWSTM QVKITSPGLF RIVYIAERHN CQYPENILSF IKCVIHNFWI PKESNEITII
     INPYRETVCF SVEPVKKIFN YMIHVNRNIM DFKLFLVFVA GVFLFFYART LSQSPTFYYS
     SGTVLGVLMT LVFVLLLVKR FIPKYSTFWA LMVGCWFASV YIVCQLMEDL KWLWYENRIY
     VLGYVLIVGF FSFVVCYKHG PLADDRSRSL LMWMLRLLSL VLVYAGVAVP QFAYAAIILL
     MSSWSLHYPL RACSYMRWKM EQWFTSKELV VKYLTEDEYR EQADAETNSA LEELRRACRK
     PDFPSWLVVS RLHTPSKFAD FVLGGSHLSP EEISLHEEQY GLGGAFLEEQ LFNPSTA
//