ID   A0A0C4DGS1_HUMAN        Unreviewed;       439 AA.
AC   A0A0C4DGS1;
DT   01-APR-2015, integrated into UniProtKB/TrEMBL.
DT   01-APR-2015, sequence version 1.
DT   13-FEB-2019, entry version 28.
DE   RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit {ECO:0000256|RuleBase:RU361142};
DE            Short=Oligosaccharyl transferase 48 kDa subunit {ECO:0000256|RuleBase:RU361142};
GN   Name=DDOST {ECO:0000313|Ensembl:ENSP00000473655};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606 {ECO:0000313|Ensembl:ENSP00000473655, ECO:0000313|Proteomes:UP000005640};
RN   [1] {ECO:0000313|Ensembl:ENSP00000473655, ECO:0000313|Proteomes:UP000005640}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16710414; DOI=10.1038/nature04727;
RA   Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D.,
RA   Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.,
RA   Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F.,
RA   McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C.,
RA   Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P.,
RA   Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I., Aubin K.,
RA   Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G.,
RA   Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D.,
RA   Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G.,
RA   Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J.,
RA   Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA   Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA   Ghori M.R., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA   Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R.,
RA   Hammond S., Harrison E.S., Hart E., Haugen E., Heath P.D., Holmes S.,
RA   Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J.,
RA   James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K.,
RA   Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G.,
RA   Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J.,
RA   Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L.,
RA   Matthews N.S., McLaren S., Milne S., Mistry S., Moore M.J.,
RA   Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A.,
RA   Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K.,
RA   Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G.,
RA   Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S.,
RA   Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N.,
RA   Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S.,
RA   Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L.,
RA   Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.,
RA   Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T.,
RA   Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R., Banerjee R.,
RA   Bryant S.P., Burford D.C., Burrill W.D., Clegg S.M., Dhami P.,
RA   Dovey O., Faulkner L.M., Gribble S.M., Langford C.F., Pandian R.D.,
RA   Porter K.M., Prigmore E.;
RT   "The DNA sequence and biological annotation of human chromosome 1.";
RL   Nature 441:315-321(2006).
RN   [2] {ECO:0000213|PubMed:21269460}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA   Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [3] {ECO:0000213|PubMed:24275569}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D.,
RA   Wang L., Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human
RT   liver phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
RN   [4] {ECO:0000213|PubMed:25944712}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=25944712;
RA   Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M.,
RA   Ayoub D., Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT   "N-terminome analysis of the human mitochondrial proteome.";
RL   Proteomics 15:2519-2524(2015).
RN   [5] {ECO:0000313|Ensembl:ENSP00000473655}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (FEB-2015) to UniProtKB.
CC   -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex
CC       that catalyzes the initial transfer of a defined glycan
CC       (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier
CC       dolichol-pyrophosphate to an asparagine residue within an Asn-X-
CC       Ser/Thr consensus motif in nascent polypeptide chains, the first
CC       step in protein N-glycosylation. N-glycosylation occurs
CC       cotranslationally and the complex associates with the Sec61
CC       complex at the channel-forming translocon complex that mediates
CC       protein translocation across the endoplasmic reticulum (ER).
CC       {ECO:0000256|RuleBase:RU361142}.
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC       {ECO:0000256|RuleBase:RU361142}.
CC   -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC       {ECO:0000256|RuleBase:RU361142}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000256|RuleBase:RU361142}; Single-pass type I membrane
CC       protein {ECO:0000256|RuleBase:RU361142}.
CC   -!- SIMILARITY: Belongs to the DDOST 48 kDa subunit family.
CC       {ECO:0000256|RuleBase:RU361142}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   EMBL; AL391357; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   UniGene; Hs.523145; -.
DR   ProteinModelPortal; A0A0C4DGS1; -.
DR   SMR; A0A0C4DGS1; -.
DR   EPD; A0A0C4DGS1; -.
DR   jPOST; A0A0C4DGS1; -.
DR   Ensembl; ENST00000602624; ENSP00000473655; ENSG00000244038.
DR   EuPathDB; HostDB:ENSG00000244038.9; -.
DR   HGNC; HGNC:2728; DDOST.
DR   OpenTargets; ENSG00000244038; -.
DR   eggNOG; KOG2754; Eukaryota.
DR   eggNOG; ENOG410XSF3; LUCA.
DR   GeneTree; ENSGT00390000017294; -.
DR   UniPathway; UPA00378; -.
DR   ChiTaRS; DDOST; human.
DR   Proteomes; UP000005640; Chromosome 1.
DR   Bgee; ENSG00000244038; Expressed in 234 organ(s), highest expression level in islet of Langerhans.
DR   ExpressionAtlas; A0A0C4DGS1; baseline and differential.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:HPA.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0018279; P:protein N-linked glycosylation via asparagine; IEA:UniProtKB-UniRule.
DR   InterPro; IPR005013; DDOST_48_kDa_subunit.
DR   PANTHER; PTHR10830; PTHR10830; 1.
DR   Pfam; PF03345; DDOST_48kD; 1.
PE   1: Evidence at protein level;
KW   Complete proteome {ECO:0000313|Proteomes:UP000005640};
KW   Endoplasmic reticulum {ECO:0000256|RuleBase:RU361142};
KW   Membrane {ECO:0000256|RuleBase:RU361142};
KW   Proteomics identification {ECO:0000213|EPD:A0A0C4DGS1,
KW   ECO:0000213|MaxQB:A0A0C4DGS1, ECO:0000213|PeptideAtlas:A0A0C4DGS1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005640};
KW   Signal {ECO:0000256|RuleBase:RU361142};
KW   Transmembrane {ECO:0000256|RuleBase:RU361142};
KW   Transmembrane helix {ECO:0000256|RuleBase:RU361142}.
FT   SIGNAL        1     25       {ECO:0000256|RuleBase:RU361142}.
FT   CHAIN        26    439       Dolichyl-diphosphooligosaccharide--
FT                                protein glycosyltransferase 48 kDa
FT                                subunit. {ECO:0000256|RuleBase:RU361142}.
FT                                /FTId=PRO_5005111353.
FT   TRANSMEM    409    430       Helical. {ECO:0000256|RuleBase:RU361142}.
SQ   SEQUENCE   439 AA;  48799 MW;  F1EB6766B6F658A0 CRC64;
     MEPSTAARAW ALFWLLLPLL GAVCASGPRT LVLLDNLNVR ETHSLFFRSL KDRGFELTFK
     TADDPSLSLI KYGEFLYDNL IIFSPSVEDF GGNINVETIS AFIDGGGSVL VAASSDIGDP
     LRELGSECGI EFDEEKTAVI DHHNYDISDL GQHTLIVADT ENLLKAPTIV GKSSLNPILF
     RGVGMVADPD NPLVLDILTG SSTSYSFFPD KPITQYPHAV GKNTLLIAGL QARNNARVIF
     SGSLDFFSDS FFNSAVQKAA PGSQRYSQTG NYELAVALSR WVFKEEGVLR VGPVSHHRVG
     ETAPPNAYTV TDLVEYSIVI QQLSNGKWVP FDGDDIQLEF VRIDPFVRTF LKKKGGKYSV
     QFKLPDVYGV FQFKVDYNRL GYTHLYSSTQ VSVRPLQHTQ YERFIPSAYP YYASAFSMML
     GLFIFSIVFL HMKEKEKSD
//