BLASTP 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: jsondb/blastdb/canonicalsequences_all.fasta 222,281 sequences; 120,184,950 total letters Query= QUERY Length=180 Score E Sequences producing significant alignments: (Bits) Value P30613-1 Isoform 1 of Pyruvate kinase PKLR OS=Homo sapiens OX=96... 249 8e-80 P53657-1 Isoform 1 of Pyruvate kinase OS=Mus musculus OX=10090 G... 221 5e-69 P12928-1 Isoform 1 of Pyruvate kinase PKLR OS=Rattus norvegicus ... 220 1e-68 A0AAA9SCU3-1 Isoform 1 of Pyruvate kinase OS=Bos taurus OX=9913 ... 214 4e-66 A0A9J7F773-1 Isoform 1 of Pyruvate kinase OS=Cricetulus griseus ... 212 3e-65 A0A286ZYV7-1 Isoform 1 of Pyruvate kinase OS=Sus scrofa OX=9823 ... 177 3e-52 A0AC58HSQ3-1 Isoform 1 of Pyruvate kinase OS=Danio rerio OX=7955... 125 7e-33 A0A9J7JQV6-1 Isoform 1 of Pyruvate kinase OS=Cricetulus griseus ... 123 5e-32 P00548-1 Isoform 1 of Pyruvate kinase PKM OS=Gallus gallus OX=90... 121 2e-31 P52480-1 Isoform 1 of Pyruvate kinase PKM OS=Mus musculus OX=100... 120 2e-31 P14618-1 Isoform 1 of Pyruvate kinase OS=Homo sapiens OX=9606 GN... 120 2e-31 P11980-1 Isoform 1 of Pyruvate kinase PKM OS=Rattus norvegicus O... 120 3e-31 A0ABK0LH17-1 Isoform 1 of Pyruvate kinase OS=Rattus norvegicus O... 120 4e-31 A0A286ZI36-1 Isoform 1 of Pyruvate kinase OS=Sus scrofa OX=9823 ... 119 2e-30 A0A8V0ZG96-1 Isoform 1 of Pyruvate kinase OS=Gallus gallus OX=90... 117 7e-30 A0A8M2BA79-1 Isoform 1 of Pyruvate kinase OS=Danio rerio OX=7955... 113 2e-28 A0A9J7GT89-1 Isoform 1 of Pyruvate kinase OS=Cricetulus griseus ... 112 2e-28 O62619-1 Isoform 1 of Pyruvate kinase OS=Drosophila melanogaster... 69.3 4e-13 Q9SQQ7-1 Isoform 1 of Pyruvate kinase OS=Arabidopsis thaliana OX... 57.8 4e-09 Q9LU95-1 Isoform 1 of Pyruvate kinase OS=Arabidopsis thaliana OX... 57.8 4e-09 Q9M057-1 Isoform 1 of Pyruvate kinase OS=Arabidopsis thaliana OX... 57.0 6e-09 Q9FFP6-1 Isoform 1 of Pyruvate kinase OS=Arabidopsis thaliana OX... 54.7 4e-08 Q9FNN1-1 Isoform 1 of Pyruvate kinase OS=Arabidopsis thaliana OX... 53.5 8e-08 Q9FM97-1 Isoform 1 of Pyruvate kinase OS=Arabidopsis thaliana OX... 51.2 6e-07 O65595-1 Isoform 1 of Probable pyruvate kinase, cytosolic isozym... 50.8 9e-07 P00549-1 Isoform 1 of Pyruvate kinase 1 OS=Saccharomyces cerevis... 48.1 6e-06 P52489-1 Isoform 1 of Pyruvate kinase 2 OS=Saccharomyces cerevis... 47.4 1e-05 Q9VQH0-1 Isoform 1 of Pyruvate kinase OS=Drosophila melanogaster... 47.0 2e-05 Q9SJQ0-1 Isoform 1 of Pyruvate kinase OS=Arabidopsis thaliana OX... 46.6 2e-05 Q54RF5-1 Isoform 1 of Pyruvate kinase OS=Dictyostelium discoideu... 45.8 3e-05 Q54RI6-1 Isoform 1 of pyruvate kinase OS=Dictyostelium discoideu... 40.8 1e-04 Q9FLW9-1 Isoform 1 of Plastidial pyruvate kinase 2 OS=Arabidopsi... 43.1 4e-04 Q93Z53-1 Isoform 1 of Plastidial pyruvate kinase 3, chloroplasti... 42.7 4e-04 >P30613-1 Isoform 1 of Pyruvate kinase PKLR OS=Homo sapiens OX=9606 GN=PKLR PE=1 SV=2 CANONICAL=P30613-1 Length=574 Score = 249 bits (637), Expect = 8e-80, Method: Compositional matrix adjust. Identities = 128/154 (83%), Positives = 133/154 (86%), Gaps = 1/154 (1%) Query 1 MSIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASVAQLTQELGTAFFQQQQ 60 MSIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASVAQLTQELGTAFFQQQQ Sbjct 1 MSIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASVAQLTQELGTAFFQQQQ 60 Query 61 LPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFS 120 LPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFS Sbjct 61 LPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFS 120 Query 121 QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEV 154 + AE+ R+ E +PLS P + Sbjct 121 HGSHEYHAESIANVREAVESF-AGSPLSYRPVAI 153 Score = 123 bits (309), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 60/60 (100%), Positives = 60/60 (100%), Gaps = 0/60 (0%) Query 121 QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRS 180 QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRS Sbjct 421 QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRS 480 >P53657-1 Isoform 1 of Pyruvate kinase OS=Mus musculus OX=10090 GN=Pklr PE=1 SV=1 CANONICAL=P53657-1 Length=574 Score = 221 bits (564), Expect = 5e-69, Method: Compositional matrix adjust. Identities = 115/154 (75%), Positives = 124/154 (81%), Gaps = 1/154 (1%) Query 1 MSIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASVAQLTQELGTAFFQQQQ 60 MS+QEN QL W+ KSQ+DLAKS L GAPGGPAGYLRRASVAQLTQELGTAFFQQQQ Sbjct 1 MSVQENELPQQLWPWIFKSQKDLAKSALSGAPGGPAGYLRRASVAQLTQELGTAFFQQQQ 60 Query 61 LPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFS 120 LPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSV+RLKEMIKAGMNIARLNFS Sbjct 61 LPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFS 120 Query 121 QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEV 154 + AE+ R+ E +PLS P + Sbjct 121 HGSHEYHAESIANIREAAESF-ATSPLSYRPVAI 153 Score = 120 bits (301), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 58/60 (97%), Positives = 59/60 (98%), Gaps = 0/60 (0%) Query 121 QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRS 180 QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEA+FKCCAAAIIVLT TGRS Sbjct 421 QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRS 480 >P12928-1 Isoform 1 of Pyruvate kinase PKLR OS=Rattus norvegicus OX=10116 GN=Pklr PE=1 SV=2 CANONICAL=P12928-1 Length=574 Score = 220 bits (561), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 113/154 (73%), Positives = 124/154 (81%), Gaps = 1/154 (1%) Query 1 MSIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASVAQLTQELGTAFFQQQQ 60 MS+QEN QL W+ +SQ+DLAKS L GAPGGPAGYLRRASVAQLTQELGTAFFQQQQ Sbjct 1 MSVQENTLPQQLWPWIFRSQKDLAKSALSGAPGGPAGYLRRASVAQLTQELGTAFFQQQQ 60 Query 61 LPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFS 120 LPAAMADTFLEHLCLLDIDS+PVAARSTSIIATIGPASRSV+RLKEMIKAGMNIARLNFS Sbjct 61 LPAAMADTFLEHLCLLDIDSQPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFS 120 Query 121 QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEV 154 + AE+ R+ E +PLS P + Sbjct 121 HGSHEYHAESIANIREATESF-ATSPLSYRPVAI 153 Score = 120 bits (301), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 58/60 (97%), Positives = 59/60 (98%), Gaps = 0/60 (0%) Query 121 QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRS 180 QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEA+FKCCAAAIIVLT TGRS Sbjct 421 QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRS 480 Lambda K H a alpha 0.320 0.129 0.357 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 6924859344 Database: jsondb/blastdb/canonicalsequences_all.fasta Posted date: Aug 13, 2026 6:42 PM Number of letters in database: 120,184,950 Number of sequences in database: 222,281 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40