{
    "title": "RNA recognition by a Staufen double-stranded RNA-binding domain.",
    "journal": "The EMBO journal",
    "date": "2000",
    "authors": "Ramos A, Grunert S, Adams J, Micklem DR, Proctor MR, Freund S, Bycroft M, St Johnston D, Varani G",
    "reference": {
        "type": "PubMed",
        "id": "10698941",
        "url": "https://pubmed.ncbi.nlm.nih.gov/10698941"
    },
    "referenced_glycans": [],
    "referenced_proteins": [
        {
            "uniprot_canonical_ac": "P25159-1",
            "protein_name": "Maternal effect protein staufen",
            "gene_name": "stau",
            "tax_name": "Fruit fly"
        }
    ],
    "species": [
        {
            "name": "Drosophila melanogaster",
            "taxid": 7227,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P25159",
                    "url": "https://www.uniprot.org/uniprot/P25159"
                }
            ],
            "common_name": "Fruit fly",
            "glygen_name": "Fruit fly",
            "reference_species": "Drosophila melanogaster [7227]"
        }
    ],
    "mutagenesis": [
        {
            "start_pos": 581,
            "end_pos": 582,
            "sequence_org": "SQ",
            "sequence_mut": "AA",
            "comment": "Affects RNA binding without altering the conformation of the domain.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P25159",
                    "url": "https://www.uniprot.org/uniprot/P25159"
                },
                {
                    "database": "PubMed",
                    "id": "10698941",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10698941"
                }
            ],
            "uniprot_canonical_ac": "P25159-1"
        },
        {
            "start_pos": 584,
            "end_pos": 585,
            "sequence_org": "HE",
            "sequence_mut": "AA",
            "comment": "Affects RNA binding without altering the conformation of the domain.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P25159",
                    "url": "https://www.uniprot.org/uniprot/P25159"
                },
                {
                    "database": "PubMed",
                    "id": "10698941",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10698941"
                }
            ],
            "uniprot_canonical_ac": "P25159-1"
        },
        {
            "start_pos": 586,
            "end_pos": 586,
            "sequence_org": "I",
            "sequence_mut": "A",
            "comment": "Crucial for the structure of DRBM 3.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P25159",
                    "url": "https://www.uniprot.org/uniprot/P25159"
                },
                {
                    "database": "PubMed",
                    "id": "10698941",
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            ],
            "uniprot_canonical_ac": "P25159-1"
        },
        {
            "start_pos": 596,
            "end_pos": 596,
            "sequence_org": "F",
            "sequence_mut": "A",
            "comment": "Crucial for the structure of DRBM 3.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P25159",
                    "url": "https://www.uniprot.org/uniprot/P25159"
                },
                {
                    "database": "PubMed",
                    "id": "10698941",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10698941"
                }
            ],
            "uniprot_canonical_ac": "P25159-1"
        },
        {
            "start_pos": 599,
            "end_pos": 602,
            "sequence_org": "LREE",
            "sequence_mut": "AAAA",
            "comment": "Strongly reduces RNA binding.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P25159",
                    "url": "https://www.uniprot.org/uniprot/P25159"
                },
                {
                    "database": "PubMed",
                    "id": "10698941",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10698941"
                }
            ],
            "uniprot_canonical_ac": "P25159-1"
        },
        {
            "start_pos": 606,
            "end_pos": 606,
            "sequence_org": "H",
            "sequence_mut": "A",
            "comment": "Abolishes RNA binding without altering the conformation of the domain.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P25159",
                    "url": "https://www.uniprot.org/uniprot/P25159"
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                {
                    "database": "PubMed",
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            ],
            "uniprot_canonical_ac": "P25159-1"
        },
        {
            "start_pos": 608,
            "end_pos": 608,
            "sequence_org": "K",
            "sequence_mut": "A",
            "comment": "Abolishes RNA binding without altering the conformation of the domain.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P25159",
                    "url": "https://www.uniprot.org/uniprot/P25159"
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                {
                    "database": "PubMed",
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            ],
            "uniprot_canonical_ac": "P25159-1"
        },
        {
            "start_pos": 628,
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            "sequence_org": "KK",
            "sequence_mut": "AA",
            "comment": "Abolishes RNA binding without altering the conformation of the domain.",
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                {
                    "database": "UniProtKB",
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            "start_pos": 632,
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                    "database": "UniProtKB",
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            "start_pos": 633,
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            "sequence_org": "KR",
            "sequence_mut": "AA",
            "comment": "No effect on RNA binding.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P25159",
                    "url": "https://www.uniprot.org/uniprot/P25159"
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            "start_pos": 635,
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            "sequence_org": "AA",
            "sequence_mut": "SS",
            "comment": "Crucial for the structure of DRBM 3.",
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    "record_id": "pubmed.10698941",
    "abstract": "",
    "glycosylation": [],
    "snv": [],
    "phosphorylation": [],
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