{ "uniprot_canonical_ac": "P36027-1", "uniprot_ac": "P36027", "uniprot_id": "MID2_YEAST", "mass": { "chemical_mass": 39146.0, "monoisotopic_mass": 39146.0 }, "refseq": { "ac": "NP_013436", "name": "Mid2p", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_013436" }, "gene_names": [ { "name": "MID2", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/P36027", "id": "P36027" }, { "name": "KAI1", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_013436", "id": "NP_013436" }, { "name": "KAI1", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/P36027", "id": "P36027" }, { "name": "L8543.19", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/P36027", "id": "P36027" }, { "name": "MID2", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_013436", "id": "NP_013436" }, { "name": "SMS1", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/P36027", "id": "P36027" } ], "protein_names": [ { "name": "Cell wall integrity sensor MID2", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprotkb/P36027/entry#names_and_taxonomy", "id": "P36027" }, { "name": "Mating pheromone-induced death protein 2", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprotkb/P36027/entry#names_and_taxonomy", "id": "P36027" }, { "name": "Mid2p", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_013436", "id": "NP_013436" }, { "name": "Protein kinase A interference protein 1", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprotkb/P36027/entry#names_and_taxonomy", "id": "P36027" }, { "name": "Serine-rich multicopy suppressor protein 1", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprotkb/P36027/entry#names_and_taxonomy", "id": "P36027" } ], "function": [ { "annotation": "Cell wall stress sensor. Involved in activation of a response that includes both stress-related increased chitin synthesis and the MPK1 mitogen-activated protein kinase cell integrity pathway. High-copy activator of the SKN7 transcription factor. Required during induction of MPK1 tyrosine phosphorylation under a variety of stress conditions. Required for PKC pathway activation under low pH conditions. Required for cell integrity signaling during pheromone-induced morphogenesis. Activates ROM1 or ROM2 catalyzed guanine nucleotide exchange toward RHO1 in cell wall integrity signaling.", "evidence": [ { "database": "UniProtKB", "id": "P36027", "url": "https://www.uniprot.org/uniprot/P36027#function" }, { "database": "PubMed", "id": "11113201", "url": "https://glygen.org/publication/PubMed/11113201" }, { "database": "PubMed", "id": "10348843", "url": "https://glygen.org/publication/PubMed/10348843" }, { "database": "PubMed", "id": "10330137", "url": "https://glygen.org/publication/PubMed/10330137" }, { "database": "PubMed", "id": "16087742", "url": "https://glygen.org/publication/PubMed/16087742" } ] } ], "disease": [], "snv": [], "mutagenesis": [], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Saccharomyces cerevisiae S288C", "taxid": 559292, "evidence": [ { "database": "UniProtKB", "id": "P36027", "url": "https://www.uniprot.org/uniprot/P36027" } ], "common_name": "Baker's yeast", "glygen_name": "Yeast", "reference_species": "Saccharomyces cerevisiae S288C [559292]" } ], "gene": [ { "name": "MID2", "url": "https://www.uniprot.org/uniprot/P36027", "locus": { "chromosome": "XII", "start_pos": 791806, "end_pos": 790676, "strand": "-", "evidence": [ { "database": "Ensembl Gene", "id": "YLR332W", "url": "https://www.ensemblgenomes.org/id/YLR332W" } ] } } ], "crossref": [ { "id": "SGD:S000004324", "url": "https://alliancegenome.org/gene/SGD:S000004324", "database": "AGR", "categories": [ "Protein Classification" ] }, { "id": "P36027", "url": "https://alphafold.ebi.ac.uk/entry/P36027", "database": "AlphaFoldDB", "categories": [] }, { "id": "851042", "url": "https://www.ncbi.nlm.nih.gov/gene/851042", "database": "GeneID", "categories": [ "Genome Annotation" ] }, { "id": "P36027", "url": "https://www.ebi.ac.uk/intact/interactors/id:P36027*", "database": "IntAct", "categories": [ "Other" ] }, { "id": "IPR007567", "url": "https://www.ebi.ac.uk/interpro/entry/IPR007567", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "sce:YLR332W", "url": "https://www.genome.jp/dbget-bin/www_bget?sce:YLR332W", "database": "KEGG Pathway", "categories": [ "Enzyme and Pathway" ] }, { "id": "SHSNELM", "url": "https://omabrowser.org/oma/group/SHSNELM/", "database": "OMA", "categories": [ "Protein Classification" ] }, { "id": "P36027", "url": "https://www.orthodb.org/?gene=P36027", "database": "OrthoDB", "categories": [ "Protein Classification" ] }, { "id": "PF04478", "url": "https://www.ebi.ac.uk/interpro/entry/pfam/PF04478", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PR_P36027", "url": "http://purl.obolibrary.org/obo/PR_P36027", "database": "PRO", "categories": [ "Protein" ] }, { "id": "NP_013436", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_013436", "database": "RefSeq", "categories": [ "Protein" ] }, { "id": "S000004324", "url": "https://www.yeastgenome.org/locus/S000004324", "database": "SGD", "categories": [ "Organism-specific" ] }, { "id": "P36027", "url": "https://www.uniprot.org/uniprot/P36027", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "P36027-1", "url": "https://www.uniprot.org/uniprot/P36027-1", "database": "UniProtKB", "categories": [ "Protein" ] } ], "sequence": { "sequence": "MLSFTTKNSFRLLLLILSCISTIRAQFFVQSSSSNSSAVSTARSSVSRVSSSSSILSSSMVSSSSADSSSLTSSTSSRSLVSHTSSSTSIASISFTSFSFSSDSSTSSSSSASSDSSSSSSFSISSTSATSESSTSSTQTSTSSSSSLSSTPSSSSSPSTITSAPSTSSTPSTTAYNQGSTITSIINGKTILSNHYTTVTYTPSATADSSNKSKSSGLSKKNRNIVIGCVVGIGVPLILVILALIYMFCIQSSRTDFIDSDGKVVTAYRANKFTKWWYMLLGKKVSDEYHSDSPLGGSASSAGGLDLDEADDVMEQSSLFDVRIRDSDSVLPNANTADHNNTNSGGEPINSSVASNDIIEEKFYDEQGNELSPRNY", "length": 376, "header": "sp|P36027-1|MID2_YEAST Isoform 1 of Cell wall integrity sensor MID2 OS=Saccharomyces cerevisiae S288C OX=559292 GN=MID2 PE=1 SV=1 CANONICAL=P36027-1" }, "isoforms": [ { "isoform_ac": "P36027-1", "url": "https://www.uniprot.org/uniprotkb/P36027/entry#P36027-1", "sequence": { "sequence": "MLSFTTKNSFRLLLLILSCISTIRAQFFVQSSSSNSSAVSTARSSVSRVSSSSSILSSSMVSSSSADSSSLTSSTSSRSLVSHTSSSTSIASISFTSFSFSSDSSTSSSSSASSDSSSSSSFSISSTSATSESSTSSTQTSTSSSSSLSSTPSSSSSPSTITSAPSTSSTPSTTAYNQGSTITSIINGKTILSNHYTTVTYTPSATADSSNKSKSSGLSKKNRNIVIGCVVGIGVPLILVILALIYMFCIQSSRTDFIDSDGKVVTAYRANKFTKWWYMLLGKKVSDEYHSDSPLGGSASSAGGLDLDEADDVMEQSSLFDVRIRDSDSVLPNANTADHNNTNSGGEPINSSVASNDIIEEKFYDEQGNELSPRNY", "length": 376, "header": "sp|P36027-1|MID2_YEAST Isoform 1 of Cell wall integrity sensor MID2 OS=Saccharomyces cerevisiae S288C OX=559292 GN=MID2 PE=1 SV=1 CANONICAL=P36027-1" }, "locus": { "chromosome": "XII", "start_pos": 791803, "end_pos": 790676, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "YLR332W_mRNA", "url": "https://www.ensemblgenomes.org/id/YLR332W_mRNA" }, { "database": "Ensembl Peptide", "id": "YLR332W", "url": "https://www.ensemblgenomes.org/id/YLR332W" } ] } } ], "glycosylation": [ { "glytoucan_ac": "", "type": "O-linked", "subtype": "other", "prediction_tool": "isoglyp", "prediction_tool_list": [ { "label": "isoglyp", "url": "https://github.com/jonmohl/ISOGlyP" } ], "site_category": "predicted", "site_category_dict": { "predicted": true }, "site_seq": "", "relation": "attached", "comment": "", "start_pos": 151, "start_aa": "T", "end_pos": 151, "end_aa": "T", "residue": "Thr", "site_lbl": "Thr151", "evidence": [ { "id": "GLY_001160", "database": "GlyGen", "url": "https://data.glygen.org/GLY_001160" } ], "filter_code": "00000000000000001000.00000000001000.1.01000" }, { "glytoucan_ac": "", "type": "O-linked", "subtype": "other", "prediction_tool": "isoglyp", "prediction_tool_list": [ { "label": "isoglyp", "url": "https://github.com/jonmohl/ISOGlyP" } ], "site_category": "predicted", "site_category_dict": { "predicted": true }, "site_seq": "", "relation": "attached", "comment": "", "start_pos": 170, "start_aa": "T", "end_pos": 170, "end_aa": "T", "residue": "Thr", "site_lbl": "Thr170", "evidence": [ { "id": "GLY_001160", "database": "GlyGen", "url": "https://data.glygen.org/GLY_001160" } ], "filter_code": "00000000000000001000.00000000001000.1.01000" }, { "glytoucan_ac": "", "type": "N-linked", "subtype": "other", "prediction_tool": "", "prediction_tool_list": [ { "label": "" } ], "site_category": "predicted", "site_category_dict": { "predicted": true }, "site_seq": "N", "relation": "attached", "comment": "N-linked (GlcNAc...) asparagine", "start_pos": 211, "start_aa": "Asn", "end_pos": 211, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn211", "evidence": [ { "id": "P36027", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P36027#ptm_processing" } ], "filter_code": "00100000000000000000.00010000000000.0.01000" }, { "glytoucan_ac": "", "type": "N-linked", "subtype": "other", "prediction_tool": "", "prediction_tool_list": [ { "label": "" } ], "site_category": "reported", "site_category_dict": { "reported": true, "automatic_literature_mining": true }, "site_seq": "N", "relation": "attached", "comment": "N-linked (GlcNAc...) asparagine", "start_pos": 35, "start_aa": "Asn", "end_pos": 35, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn35", "evidence": [ { "id": "P36027", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P36027#ptm_processing" }, { "id": "18410496", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/18410496" }, { "id": "GLY_001402", "database": "GlyGen", "url": "https://data.glygen.org/GLY_001402" } ], "mining_tool": "GlycoSiteMiner", "mining_tool_list": [ { "label": "GlycoSiteMiner", "url": "https://github.com/glygener/glycositeminer" } ], "filter_code": "00100000000000000000.00010000000000.01.01000" } ], "phosphorylation": [ { "start_pos": 260, "end_pos": 260, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "Phosphoserine", "evidence": [ { "database": "UniProtKB", "id": "P36027", "url": "https://www.uniprot.org/uniprot/P36027" }, { "database": "PubMed", "id": "19779198", "url": 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"https://www.yeastgenome.org/locus/P36027" } ], "site_lbl": "Thr266" }, { "start_pos": 268, "end_pos": 268, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Tyr", "comment": "", "evidence": [ { "database": "iPTMnet", "id": "P36027", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P36027" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "SGD", "id": "P36027", "url": "https://www.yeastgenome.org/locus/P36027" } ], "site_lbl": "Tyr268" }, { "start_pos": 291, "end_pos": 291, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "SGD", "id": "P36027", "url": "https://www.yeastgenome.org/locus/P36027" }, { "database": "iPTMnet", "id": "P36027", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P36027" }, { "database": "PubMed", "id": "33491328", "url": "https://glygen.org/publication/PubMed/33491328" } ], "site_lbl": "Ser291" }, { "start_pos": 301, "end_pos": 301, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "SGD", "id": "P36027", "url": "https://www.yeastgenome.org/locus/P36027" }, { "database": "iPTMnet", "id": "P36027", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P36027" }, { "database": "PubMed", "id": "33491328", "url": "https://glygen.org/publication/PubMed/33491328" } ], "site_lbl": "Ser301" }, { "start_pos": 327, "end_pos": 327, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "Phosphoserine", "evidence": [ { "database": "UniProtKB", "id": "P36027", "url": "https://www.uniprot.org/uniprot/P36027" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "iPTMnet", "id": "P36027", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P36027" }, { "database": "PubMed", "id": "20702584", "url": "https://glygen.org/publication/PubMed/20702584" }, { "database": "SGD", "id": "P36027", "url": "https://www.yeastgenome.org/locus/P36027" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" }, { "database": "PubMed", "id": "33491328", "url": "https://glygen.org/publication/PubMed/33491328" } ], "site_lbl": "Ser327" }, { "start_pos": 329, "end_pos": 329, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "Phosphoserine", "evidence": [ { "database": "UniProtKB", "id": "P36027", "url": "https://www.uniprot.org/uniprot/P36027" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "iPTMnet", "id": "P36027", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P36027" }, { "database": "SGD", "id": "P36027", "url": "https://www.yeastgenome.org/locus/P36027" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ], "site_lbl": "Ser329" }, { "start_pos": 336, "end_pos": 336, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Thr", "comment": "", "evidence": [ { "database": "iPTMnet", "id": "P36027", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P36027" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "SGD", "id": "P36027", "url": "https://www.yeastgenome.org/locus/P36027" } ], "site_lbl": "Thr336" }, { "start_pos": 342, "end_pos": 342, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Thr", "comment": "", "evidence": [ { "database": "SGD", "id": "P36027", "url": "https://www.yeastgenome.org/locus/P36027" }, { "database": "iPTMnet", "id": "P36027", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P36027" }, { "database": "PubMed", "id": "33491328", "url": "https://glygen.org/publication/PubMed/33491328" } ], "site_lbl": "Thr342" }, { "start_pos": 351, "end_pos": 351, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "iPTMnet", "id": "P36027", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P36027" }, { "database": "PubMed", "id": "20702584", "url": "https://glygen.org/publication/PubMed/20702584" }, { "database": "SGD", "id": "P36027", "url": "https://www.yeastgenome.org/locus/P36027" }, { "database": "PubMed", "id": "33491328", "url": "https://glygen.org/publication/PubMed/33491328" } ], "site_lbl": "Ser351" }, { "start_pos": 352, "end_pos": 352, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "iPTMnet", "id": "P36027", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P36027" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "PubMed", "id": "20702584", "url": "https://glygen.org/publication/PubMed/20702584" }, { "database": "SGD", "id": "P36027", "url": "https://www.yeastgenome.org/locus/P36027" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" }, { "database": "PubMed", "id": "33491328", "url": "https://glygen.org/publication/PubMed/33491328" } ], "site_lbl": "Ser352" }, { "start_pos": 355, "end_pos": 355, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "Phosphoserine", "evidence": [ { "database": "UniProtKB", "id": "P36027", "url": "https://www.uniprot.org/uniprot/P36027" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "iPTMnet", "id": "P36027", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P36027" }, { "database": "PubMed", "id": "20702584", "url": "https://glygen.org/publication/PubMed/20702584" }, { "database": "SGD", "id": "P36027", "url": "https://www.yeastgenome.org/locus/P36027" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" }, { "database": "PubMed", "id": "33491328", "url": "https://glygen.org/publication/PubMed/33491328" } ], "site_lbl": "Ser355" }, { "start_pos": 372, "end_pos": 372, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "SGD", "id": "P36027", "url": "https://www.yeastgenome.org/locus/P36027" }, { "database": "iPTMnet", "id": "P36027", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P36027" }, { "database": "PubMed", "id": "22218487", "url": "https://glygen.org/publication/PubMed/22218487" }, { "database": "PubMed", "id": "32597660", "url": "https://glygen.org/publication/PubMed/32597660" }, { "database": "PubMed", "id": "33481703", "url": "https://glygen.org/publication/PubMed/33481703" }, { "database": "PubMed", "id": "33491328", "url": "https://glygen.org/publication/PubMed/33491328" } ], "site_lbl": "Ser372" } ], "glycation": [], "keywords": [ "glycoprotein", "glycoprotein_predicted", "glycoprotein_reported" ], "publication": [ { "title": "Mid2 is a putative sensor for cell integrity signaling in Saccharomyces cerevisiae.", "journal": "Molecular and cellular biology", "date": "1999-06", "authors": "Rajavel M, Philip B, Buehrer B M, Errede B, Levin D E", "evidence": [ { "database": "UniProtKB", "id": "P36027", "url": "https://www.uniprot.org/uniprot/P36027" } ], "reference": [ { "type": "PubMed", "id": "10330137", "url": "https://glygen.org/publication/PubMed/10330137" } ] }, { "title": "Saccharomyces cerevisiae mid2p is a potential cell wall stress sensor and upstream activator of the PKC1-MPK1 cell integrity pathway.", "journal": "Journal of bacteriology", "date": "1999-06", "authors": "Ketela T, Green R, Bussey H", "evidence": [ { "database": "UniProtKB", "id": "P36027", "url": "https://www.uniprot.org/uniprot/P36027" } ], "reference": [ { "type": "PubMed", "id": "10348843", "url": "https://glygen.org/publication/PubMed/10348843" } ] }, { "title": "Wsc1 and Mid2 are cell surface sensors for cell wall integrity signaling that act through Rom2, a guanine nucleotide exchange factor for Rho1.", "journal": "Molecular and cellular biology", "date": "2001-01", "authors": "Philip B, Levin D E", "evidence": [ { "database": "UniProtKB", "id": "P36027", "url": "https://www.uniprot.org/uniprot/P36027" } ], "reference": [ { "type": "PubMed", "id": "11113201", "url": "https://glygen.org/publication/PubMed/11113201" } ] }, { "title": "The Rgd1p Rho GTPase-activating protein and the Mid2p cell wall sensor are required at low pH for protein kinase C pathway activation and cell survival in Saccharomyces cerevisiae.", "journal": "Eukaryotic cell", "date": "2005-08", "authors": "Claret Sandra, Gatti Xavier, Doignon Fran\u00e7ois, Thoraval Didier, Crouzet Marc", "evidence": [ { 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