{
    "title": "The N-terminal anchor sequences of 11beta-hydroxysteroid dehydrogenases determine their orientation in the endoplasmic reticulum membrane.",
    "journal": "The Journal of biological chemistry",
    "date": "1999",
    "authors": "Odermatt A, Arnold P, Stauffer A, Frey BM, Frey FJ",
    "reference": {
        "type": "PubMed",
        "id": "10497248",
        "url": "https://pubmed.ncbi.nlm.nih.gov/10497248"
    },
    "referenced_glycans": [],
    "referenced_proteins": [
        {
            "uniprot_canonical_ac": "P80365-1",
            "protein_name": "11-beta-hydroxysteroid dehydrogenase type 2",
            "gene_name": "HSD11B2",
            "tax_name": "Human"
        },
        {
            "uniprot_canonical_ac": "P28845-1",
            "protein_name": "11-beta-hydroxysteroid dehydrogenase 1",
            "gene_name": "HSD11B1",
            "tax_name": "Human"
        }
    ],
    "species": [
        {
            "name": "Homo sapiens",
            "taxid": 9606,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P80365",
                    "url": "https://www.uniprot.org/uniprot/P80365"
                }
            ],
            "common_name": "Human",
            "glygen_name": "Human",
            "reference_species": "Homo sapiens [9606]"
        }
    ],
    "mutagenesis": [
        {
            "start_pos": 18,
            "end_pos": 21,
            "sequence_org": "YYYY",
            "sequence_mut": "AAAA",
            "comment": "No effect on topology. Reduced Vmax.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P28845",
                    "url": "https://www.uniprot.org/uniprot/P28845"
                },
                {
                    "database": "PubMed",
                    "id": "10497248",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10497248"
                }
            ],
            "uniprot_canonical_ac": "P28845-1"
        },
        {
            "start_pos": 18,
            "end_pos": 21,
            "sequence_org": "YYYY",
            "sequence_mut": "FFFF",
            "comment": "No effect on topology or activity.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P28845",
                    "url": "https://www.uniprot.org/uniprot/P28845"
                },
                {
                    "database": "PubMed",
                    "id": "10497248",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10497248"
                }
            ],
            "uniprot_canonical_ac": "P28845-1"
        },
        {
            "start_pos": 19,
            "end_pos": 21,
            "sequence_org": "YYY",
            "sequence_mut": "AYA",
            "comment": "No effect on topology. Reduced Vmax.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P28845",
                    "url": "https://www.uniprot.org/uniprot/P28845"
                },
                {
                    "database": "PubMed",
                    "id": "10497248",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10497248"
                }
            ],
            "uniprot_canonical_ac": "P28845-1"
        },
        {
            "start_pos": 5,
            "end_pos": 5,
            "sequence_org": "K",
            "sequence_mut": "R",
            "comment": "Predominantly inverted topology. No effect on activity.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P28845",
                    "url": "https://www.uniprot.org/uniprot/P28845"
                },
                {
                    "database": "PubMed",
                    "id": "10497248",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10497248"
                }
            ],
            "uniprot_canonical_ac": "P28845-1"
        },
        {
            "start_pos": 5,
            "end_pos": 5,
            "sequence_org": "K",
            "sequence_mut": "S",
            "comment": "Inverted topology. No effect on activity.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P28845",
                    "url": "https://www.uniprot.org/uniprot/P28845"
                },
                {
                    "database": "PubMed",
                    "id": "10497248",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10497248"
                }
            ],
            "uniprot_canonical_ac": "P28845-1"
        },
        {
            "start_pos": 5,
            "end_pos": 6,
            "sequence_org": "KK",
            "sequence_mut": "RR",
            "comment": "Predominantly inverted topology. No effect on activity.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P28845",
                    "url": "https://www.uniprot.org/uniprot/P28845"
                },
                {
                    "database": "PubMed",
                    "id": "10497248",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10497248"
                },
                {
                    "database": "PubMed",
                    "id": "15152005",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/15152005"
                }
            ],
            "uniprot_canonical_ac": "P28845-1"
        },
        {
            "start_pos": 5,
            "end_pos": 6,
            "sequence_org": "KK",
            "sequence_mut": "SS",
            "comment": "Inverted topology. Reduced Vmax.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P28845",
                    "url": "https://www.uniprot.org/uniprot/P28845"
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                {
                    "database": "PubMed",
                    "id": "10497248",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10497248"
                },
                {
                    "database": "PubMed",
                    "id": "15152005",
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            "uniprot_canonical_ac": "P28845-1"
        },
        {
            "start_pos": 6,
            "end_pos": 6,
            "sequence_org": "K",
            "sequence_mut": "R",
            "comment": "No effect on topology. Increased Km for corticosterone.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P28845",
                    "url": "https://www.uniprot.org/uniprot/P28845"
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                    "database": "PubMed",
                    "id": "10497248",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10497248"
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            "uniprot_canonical_ac": "P28845-1"
        },
        {
            "start_pos": 6,
            "end_pos": 6,
            "sequence_org": "K",
            "sequence_mut": "S",
            "comment": "No effect on topology or activity.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P28845",
                    "url": "https://www.uniprot.org/uniprot/P28845"
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    "abstract": "",
    "glycosylation": [],
    "snv": [],
    "phosphorylation": [],
    "glycation": [],
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