{
    "title": "Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.",
    "journal": "Nature",
    "date": "1999",
    "authors": "Carazo-Salas RE, Guarguaglini G, Gruss OJ, Segref A, Karsenti E, Mattaj IW",
    "reference": {
        "type": "PubMed",
        "id": "10408446",
        "url": "https://pubmed.ncbi.nlm.nih.gov/10408446"
    },
    "referenced_glycans": [],
    "referenced_proteins": [
        {
            "uniprot_canonical_ac": "P62826-1",
            "protein_name": "GTP-binding nuclear protein Ran",
            "gene_name": "RAN",
            "tax_name": "Human"
        }
    ],
    "species": [
        {
            "name": "Homo sapiens",
            "taxid": 9606,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P62826",
                    "url": "https://www.uniprot.org/uniprot/P62826"
                }
            ],
            "common_name": "Human",
            "glygen_name": "Human",
            "reference_species": "Homo sapiens [9606]"
        }
    ],
    "mutagenesis": [
        {
            "start_pos": 24,
            "end_pos": 24,
            "sequence_org": "T",
            "sequence_mut": "N",
            "comment": "Has low binding affinity for GTP and GDP. Decreases nuclear import of proteins and RNA. Stabilizes the interaction with RCC1. No effect on nuclear location during interphase. Loss of activity in triggering microtubule assembly at mitotic chromosomes.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P62826",
                    "url": "https://www.uniprot.org/uniprot/P62826"
                },
                {
                    "database": "PubMed",
                    "id": "10408446",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10408446"
                },
                {
                    "database": "PubMed",
                    "id": "9351834",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/9351834"
                },
                {
                    "database": "PubMed",
                    "id": "7819259",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/7819259"
                },
                {
                    "database": "PubMed",
                    "id": "12194828",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/12194828"
                },
                {
                    "database": "PubMed",
                    "id": "8636225",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/8636225"
                }
            ],
            "uniprot_canonical_ac": "P62826-1"
        },
        {
            "start_pos": 69,
            "end_pos": 69,
            "sequence_org": "Q",
            "sequence_mut": "L",
            "comment": "Strongly decreased GTPase activity. Probably locked in the GTP-bound form. Loss of interaction with NUTF2. Decreases nuclear location and leads to cytoplasmic location during interphase. Loss of function in importin-mediated protein nuclear import. High activity in triggering microtubule assembly at mitotic chromosomes. Blocks DNA replication; when associated with V-19.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P62826",
                    "url": "https://www.uniprot.org/uniprot/P62826"
                },
                {
                    "database": "PubMed",
                    "id": "8636225",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/8636225"
                },
                {
                    "database": "PubMed",
                    "id": "7819259",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/7819259"
                },
                {
                    "database": "PubMed",
                    "id": "12194828",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/12194828"
                },
                {
                    "database": "PubMed",
                    "id": "18591255",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/18591255"
                },
                {
                    "database": "PubMed",
                    "id": "10408446",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10408446"
                },
                {
                    "database": "PubMed",
                    "id": "8421051",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/8421051"
                },
                {
                    "database": "PubMed",
                    "id": "17209048",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/17209048"
                },
                {
                    "database": "PubMed",
                    "id": "9351834",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/9351834"
                },
                {
                    "database": "PubMed",
                    "id": "9822603",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/9822603"
                }
            ],
            "uniprot_canonical_ac": "P62826-1"
        }
    ],
    "record_id": "pubmed.10408446",
    "abstract": "",
    "glycosylation": [],
    "snv": [],
    "phosphorylation": [],
    "glycation": [],
    "section_stats": [
        {
            "table_id": "referenced_proteins",
            "table_stats": [
                {
                    "field": "total",
                    "count": 1
                }
            ],
            "sort_fields": [
                "uniprot_canonical_ac",
                "protein_name",
                "gene_name",
                "tax_name"
            ]
        },
        {
            "table_id": "referenced_glycans",
            "table_stats": [
                {
                    "field": "total",
                    "count": 0
                }
            ],
            "sort_fields": []
        },
        {
            "table_id": "glycosylation",
            "table_stats": [
                {
                    "field": "total",
                    "count": 0
                },
                {
                    "field": "total_sites",
                    "count": 0
                },
                {
                    "field": "n_linked_glycans",
                    "count": 0
                },
                {
                    "field": "n_linked_glycan_sites",
                    "count": 0
                },
                {
                    "field": "o_linked_glycans",
                    "count": 0
                },
                {
                    "field": "o_linked_glycan_sites",
                    "count": 0
                }
            ],
            "sort_fields": []
        },
        {
            "table_id": "phosphorylation",
            "table_stats": [
                {
                    "field": "total",
                    "count": 0
                },
                {
                    "field": "total_sites",
                    "count": 0
                }
            ],
            "sort_fields": []
        },
        {
            "table_id": "glycosylation_reported",
            "table_stats": [
                {
                    "field": "total",
                    "count": 0
                },
                {
                    "field": "total_sites",
                    "count": 0
                },
                {
                    "field": "n_linked_glycans",
                    "count": 0
                },
                {
                    "field": "n_linked_glycan_sites",
                    "count": 0
                },
                {
                    "field": "o_linked_glycans",
                    "count": 0
                },
                {
                    "field": "o_linked_glycan_sites",
                    "count": 0
                }
            ],
            "sort_fields": []
        },
        {
            "table_id": "glycosylation_reported_with_glycan",
            "table_stats": [
                {
                    "field": "total",
                    "count": 0
                },
                {
                    "field": "total_sites",
                    "count": 0
                },
                {
                    "field": "n_linked_glycans",
                    "count": 0
                },
                {
                    "field": "n_linked_glycan_sites",
                    "count": 0
                },
                {
                    "field": "o_linked_glycans",
                    "count": 0
                },
                {
                    "field": "o_linked_glycan_sites",
                    "count": 0
                }
            ],
            "sort_fields": []
        },
        {
            "table_id": "glycosylation_predicted",
            "table_stats": [
                {
                    "field": "total",
                    "count": 0
                },
                {
                    "field": "total_sites",
                    "count": 0
                },
                {
                    "field": "n_linked_glycans",
                    "count": 0
                },
                {
                    "field": "n_linked_glycan_sites",
                    "count": 0
                },
                {
                    "field": "o_linked_glycans",
                    "count": 0
                },
                {
                    "field": "o_linked_glycan_sites",
                    "count": 0
                }
            ],
            "sort_fields": []
        },
        {
            "table_id": "glycosylation_automatic_literature_mining",
            "table_stats": [
                {
                    "field": "total",
                    "count": 0
                },
                {
                    "field": "total_sites",
                    "count": 0
                },
                {
                    "field": "n_linked_glycans",
                    "count": 0
                },
                {
                    "field": "n_linked_glycan_sites",
                    "count": 0
                },
                {
                    "field": "o_linked_glycans",
                    "count": 0
                },
                {
                    "field": "o_linked_glycan_sites",
                    "count": 0
                }
            ],
            "sort_fields": []
        }
    ]
}