{
    "title": "A structural explanation for the binding of multiple ligands by the alpha-adaptin appendage domain.",
    "journal": "Cell",
    "date": "1999",
    "authors": "Owen DJ, Vallis Y, Noble ME, Hunter JB, Dafforn TR, Evans PR, McMahon HT",
    "reference": {
        "type": "PubMed",
        "id": "10380931",
        "url": "https://pubmed.ncbi.nlm.nih.gov/10380931"
    },
    "referenced_glycans": [],
    "referenced_proteins": [
        {
            "uniprot_canonical_ac": "O88339-1",
            "protein_name": "Epsin-1",
            "gene_name": "Epn1",
            "tax_name": "Rat"
        },
        {
            "uniprot_canonical_ac": "P17427-1",
            "protein_name": "AP-2 complex subunit alpha-2",
            "gene_name": "Ap2a2",
            "tax_name": "Mouse"
        },
        {
            "uniprot_canonical_ac": "O08839-1",
            "protein_name": "Myc box-dependent-interacting protein 1",
            "gene_name": "Bin1",
            "tax_name": "Rat"
        },
        {
            "uniprot_canonical_ac": "Q05140-1",
            "protein_name": "Clathrin coat assembly protein AP180",
            "gene_name": "Snap91",
            "tax_name": "Rat"
        },
        {
            "uniprot_canonical_ac": "O08838-1",
            "protein_name": "Amphiphysin",
            "gene_name": "Amph",
            "tax_name": "Rat"
        }
    ],
    "species": [
        {
            "name": "Rattus norvegicus",
            "taxid": 10116,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "O88339",
                    "url": "https://www.uniprot.org/uniprot/O88339"
                }
            ],
            "common_name": "Rat",
            "glygen_name": "Rat",
            "reference_species": "Rattus norvegicus [10116]"
        }
    ],
    "mutagenesis": [
        {
            "start_pos": 837,
            "end_pos": 837,
            "sequence_org": "F",
            "sequence_mut": "A",
            "comment": "Reduces SNAP91, AMPH and BIN1 binding. Abolishes AMPH and SNAP91 binding; when associated with A-916. Abolishes EPN1 and EPS15 binding; when associated with A-905.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P17427",
                    "url": "https://www.uniprot.org/uniprot/P17427"
                },
                {
                    "database": "PubMed",
                    "id": "10430869",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10430869"
                },
                {
                    "database": "PubMed",
                    "id": "10380931",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10380931"
                }
            ],
            "uniprot_canonical_ac": "P17427-1"
        },
        {
            "start_pos": 840,
            "end_pos": 840,
            "sequence_org": "W",
            "sequence_mut": "A",
            "comment": "Abolishes AMPH, BIN1, DGKD, EPS15, EPN1, auxilin and SNAP91 binding.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P17427",
                    "url": "https://www.uniprot.org/uniprot/P17427"
                },
                {
                    "database": "PubMed",
                    "id": "17880279",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/17880279"
                },
                {
                    "database": "PubMed",
                    "id": "10380931",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10380931"
                }
            ],
            "uniprot_canonical_ac": "P17427-1"
        },
        {
            "start_pos": 849,
            "end_pos": 849,
            "sequence_org": "E",
            "sequence_mut": "A",
            "comment": "No effect.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P17427",
                    "url": "https://www.uniprot.org/uniprot/P17427"
                },
                {
                    "database": "PubMed",
                    "id": "10380931",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10380931"
                }
            ],
            "uniprot_canonical_ac": "P17427-1"
        },
        {
            "start_pos": 905,
            "end_pos": 905,
            "sequence_org": "R",
            "sequence_mut": "A",
            "comment": "Strongly reduces AMPH, SNAP91, auxilin and BIN1 binding. Abolishes EPN1 and EPS15 binding; when associated with A-837.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P17427",
                    "url": "https://www.uniprot.org/uniprot/P17427"
                },
                {
                    "database": "PubMed",
                    "id": "10430869",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10430869"
                },
                {
                    "database": "PubMed",
                    "id": "10380931",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10380931"
                }
            ],
            "uniprot_canonical_ac": "P17427-1"
        },
        {
            "start_pos": 907,
            "end_pos": 907,
            "sequence_org": "E",
            "sequence_mut": "A",
            "comment": "Strongly reduces AMPH, SNAP91 and BIN1 binding. Slightly reduces EPS15 and auxilin binding.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P17427",
                    "url": "https://www.uniprot.org/uniprot/P17427"
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                    "database": "PubMed",
                    "id": "10380931",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10380931"
                }
            ],
            "uniprot_canonical_ac": "P17427-1"
        },
        {
            "start_pos": 920,
            "end_pos": 920,
            "sequence_org": "R",
            "sequence_mut": "A",
            "comment": "Abolishes AMPH and BIN1 binding. Reduces EPS15, SNAP91 and auxilin binding.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P17427",
                    "url": "https://www.uniprot.org/uniprot/P17427"
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                    "database": "PubMed",
                    "id": "10380931",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10380931"
                }
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            "uniprot_canonical_ac": "P17427-1"
        }
    ],
    "record_id": "pubmed.10380931",
    "abstract": "",
    "glycosylation": [],
    "snv": [],
    "phosphorylation": [],
    "glycation": [],
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