{
    "title": "The yeast poly(A)-binding protein Pab1p stimulates in vitro poly(A)-dependent and cap-dependent translation by distinct mechanisms.",
    "journal": "The EMBO journal",
    "date": "1999-06-01",
    "authors": "Otero L J, Ashe M P, Sachs A B",
    "reference": {
        "type": "PubMed",
        "id": "10357826",
        "url": "https://pubmed.ncbi.nlm.nih.gov/10357826"
    },
    "referenced_glycans": [],
    "referenced_proteins": [
        {
            "uniprot_canonical_ac": "P39936-1",
            "protein_name": "Eukaryotic initiation factor 4F subunit p130",
            "gene_name": "TIF4632",
            "tax_name": "Baker's yeast"
        },
        {
            "uniprot_canonical_ac": "P39935-1",
            "protein_name": "Eukaryotic initiation factor 4F subunit p150",
            "gene_name": "TIF4631",
            "tax_name": "Baker's yeast"
        },
        {
            "uniprot_canonical_ac": "P04147-1",
            "protein_name": "Polyadenylate-binding protein, cytoplasmic and nuclear",
            "gene_name": "PAB1",
            "tax_name": "Baker's yeast"
        }
    ],
    "species": [
        {
            "name": "Saccharomyces cerevisiae S288C",
            "taxid": 559292,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P39936",
                    "url": "https://www.uniprot.org/uniprot/P39936"
                }
            ],
            "common_name": "Baker's yeast",
            "glygen_name": "Yeast",
            "reference_species": "Saccharomyces cerevisiae S288C [559292]"
        }
    ],
    "mutagenesis": [
        {
            "start_pos": 134,
            "end_pos": 136,
            "sequence_org": "HPD",
            "sequence_mut": "DKS",
            "comment": "In PAB1-134.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P04147",
                    "url": "https://www.uniprot.org/uniprot/P04147"
                },
                {
                    "database": "PubMed",
                    "id": "10357826",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10357826"
                }
            ],
            "uniprot_canonical_ac": "P04147-1"
        },
        {
            "start_pos": 148,
            "end_pos": 148,
            "sequence_org": "V",
            "sequence_mut": "A",
            "comment": "In PAB1-148; greatly reduces poly(A)-dependent translation and moderately reduces stimulation of cap-dependent translation in vitro; when associated with N-151.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P04147",
                    "url": "https://www.uniprot.org/uniprot/P04147"
                },
                {
                    "database": "PubMed",
                    "id": "10357826",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10357826"
                }
            ],
            "uniprot_canonical_ac": "P04147-1"
        },
        {
            "start_pos": 151,
            "end_pos": 151,
            "sequence_org": "D",
            "sequence_mut": "N",
            "comment": "In PAB1-148; greatly reduces poly(A)-dependent translation and moderately reduces stimulation of cap-dependent translation in vitro; when associated with A-148.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P04147",
                    "url": "https://www.uniprot.org/uniprot/P04147"
                },
                {
                    "database": "PubMed",
                    "id": "10357826",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10357826"
                }
            ],
            "uniprot_canonical_ac": "P04147-1"
        },
        {
            "start_pos": 157,
            "end_pos": 159,
            "sequence_org": "IAT",
            "sequence_mut": "VVC",
            "comment": "In PAB1-157; greatly reduces poly(A)-dependent translation and stimulation of cap-dependent translation in vitro.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P04147",
                    "url": "https://www.uniprot.org/uniprot/P04147"
                },
                {
                    "database": "PubMed",
                    "id": "10357826",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10357826"
                }
            ],
            "uniprot_canonical_ac": "P04147-1"
        },
        {
            "start_pos": 175,
            "end_pos": 177,
            "sequence_org": "EEG",
            "sequence_mut": "TQE",
            "comment": "In PAB1-175; greatly reduces poly(A)-dependent translation and stimulation of cap-dependent translation in vitro.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P04147",
                    "url": "https://www.uniprot.org/uniprot/P04147"
                },
                {
                    "database": "PubMed",
                    "id": "10357826",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10357826"
                }
            ],
            "uniprot_canonical_ac": "P04147-1"
        },
        {
            "start_pos": 180,
            "end_pos": 181,
            "sequence_org": "KE",
            "sequence_mut": "ER",
            "comment": "In PAB1-180; abolishes poly(A)-dependent translation and greatly reduces stimulation of cap-dependent translation in vitro. Impairs interaction with eIF4G.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P04147",
                    "url": "https://www.uniprot.org/uniprot/P04147"
                },
                {
                    "database": "PubMed",
                    "id": "10357826",
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            "uniprot_canonical_ac": "P04147-1"
        },
        {
            "start_pos": 184,
            "end_pos": 186,
            "sequence_org": "DAL",
            "sequence_mut": "EKM",
            "comment": "In PAB1-184; abolishes poly(A)-dependent translation and moderately reduces stimulation of cap-dependent translation in vitro. Impairs interaction with eIF4G.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P04147",
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        {
            "start_pos": 193,
            "end_pos": 197,
            "sequence_org": "GQEIY",
            "sequence_mut": "DRKVF",
            "comment": "In PAB1-193; moderately reduces stimulation of cap-dependent translation in vitro.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P04147",
                    "url": "https://www.uniprot.org/uniprot/P04147"
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            "uniprot_canonical_ac": "P04147-1"
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            "start_pos": 199,
            "end_pos": 202,
            "sequence_org": "APHL",
            "sequence_mut": "GRFK",
            "comment": "In PAB1-199; moderately reduces poly(A)-dependent translation and stimulation of cap-dependent translation in vitro.",
            "evidence": [
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                    "database": "UniProtKB",
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    "record_id": "pubmed.10357826",
    "abstract": "",
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    "snv": [],
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