{
    "title": "Insulin stimulates phosphorylation of the forkhead transcription factor FKHR on serine 253 through a Wortmannin-sensitive pathway.",
    "journal": "The Journal of biological chemistry",
    "date": "1999",
    "authors": "Nakae J, Park BC, Accili D",
    "reference": {
        "type": "PubMed",
        "id": "10347145",
        "url": "https://pubmed.ncbi.nlm.nih.gov/10347145"
    },
    "referenced_glycans": [],
    "referenced_proteins": [
        {
            "uniprot_canonical_ac": "Q9R1E0-1",
            "protein_name": "Forkhead box protein O1",
            "gene_name": "Foxo1",
            "tax_name": "Mouse"
        }
    ],
    "species": [
        {
            "name": "Mus musculus",
            "taxid": 10090,
            "evidence": [
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                    "id": "Q9R1E0",
                    "url": "https://www.uniprot.org/uniprot/Q9R1E0"
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            ],
            "common_name": "Mouse",
            "glygen_name": "Mouse",
            "reference_species": "Mus musculus [10090]"
        }
    ],
    "mutagenesis": [
        {
            "start_pos": 24,
            "end_pos": 24,
            "sequence_org": "T",
            "sequence_mut": "A",
            "comment": "Decreases insulin-induced phosphorylation by approximately 30%. Nuclear location but transcriptional activity decreased by about 50%. Abolishes the SIRT1 deacetylase binding and increases acetylation; when associated with A-253; A-316; A-462 and A-463. Increased insulin-induced phosphorylation at Ser-253 and binding of 14-3-3 proteins; when associated with Q-219; Q-242; Q-245; Q-259; Q-262; Q-271 and Q-291. Increased binding of 14-3-3 proteins even with decreased insulin-induced phosphorylation at Ser-253; when associated with R-219; R-242; R-245; R-259; R-262; R-271 and R-291.",
            "evidence": [
                {
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            "sequence_org": "S",
            "sequence_mut": "A",
            "comment": "Abolishes insulin-induced phosphorylation when associated with A-463. Nuclear location but transcriptional activity decreased by about 50%. Abolishes the SIRT1 deacetylase binding and increases acetylation; when associated with A-24; A-316; A-462 and A-463.",
            "evidence": [
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        {
            "start_pos": 316,
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            "sequence_org": "S",
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            "comment": "Decreases insulin-induced phosphorylation by approximately 30%. Abolishes the SIRT1 deacetylase binding and increases acetylation; when associated with A-24; A-253; A-462 and A-463.",
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            "end_pos": 24,
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            "kinase_gene_name": "",
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            "comment": "Phosphothreonine; by PKB/AKT1 or PKB/AKT2 and SGK1",
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                {
                    "database": "PubMed",
                    "id": "24466179",
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                    "id": "28267764",
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            ],
            "site_lbl": "Thr24",
            "uniprot_canonical_ac": "Q9R1E0-1"
        },
        {
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            "end_pos": 253,
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            "comment": "Phosphoserine; by PKB/AKT1 and SGK1",
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            "site_lbl": "Ser253",
            "uniprot_canonical_ac": "Q9R1E0-1"
        },
        {
            "start_pos": 316,
            "end_pos": 316,
            "kinase_uniprot_canonical_ac": "",
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            "residue": "Ser",
            "comment": "Phosphoserine; by PKB/AKT1 or PKB/AKT2",
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    "abstract": "",
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