{
    "title": "Apg7p/Cvt2p: A novel protein-activating enzyme essential for autophagy.",
    "journal": "Molecular biology of the cell",
    "date": "1999-05",
    "authors": "Tanida I, Mizushima N, Kiyooka M, Ohsumi M, Ueno T, Ohsumi Y, Kominami E",
    "reference": {
        "type": "PubMed",
        "id": "10233150",
        "url": "https://pubmed.ncbi.nlm.nih.gov/10233150"
    },
    "referenced_glycans": [],
    "referenced_proteins": [
        {
            "uniprot_canonical_ac": "P38862-1",
            "protein_name": "Ubiquitin-like modifier-activating enzyme ATG7",
            "gene_name": "ATG7",
            "tax_name": "Baker's yeast"
        },
        {
            "uniprot_canonical_ac": "P38316-1",
            "protein_name": "Ubiquitin-like protein ATG12",
            "gene_name": "ATG12",
            "tax_name": "Baker's yeast"
        }
    ],
    "species": [
        {
            "name": "Saccharomyces cerevisiae S288C",
            "taxid": 559292,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P38862",
                    "url": "https://www.uniprot.org/uniprot/P38862"
                }
            ],
            "common_name": "Baker's yeast",
            "glygen_name": "Yeast",
            "reference_species": "Saccharomyces cerevisiae S288C [559292]"
        }
    ],
    "mutagenesis": [
        {
            "start_pos": 333,
            "end_pos": 333,
            "sequence_org": "G",
            "sequence_mut": "A",
            "comment": "Loss of interaction with ATG8 and ATG12, and no more ATG12-ATG5 conjugate. Defect in Cvt pathway and autophagy.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P38862",
                    "url": "https://www.uniprot.org/uniprot/P38862"
                },
                {
                    "database": "PubMed",
                    "id": "10233150",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10233150"
                },
                {
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                    "url": "https://pubmed.ncbi.nlm.nih.gov/11100732"
                }
            ],
            "uniprot_canonical_ac": "P38862-1"
        },
        {
            "start_pos": 507,
            "end_pos": 507,
            "sequence_org": "C",
            "sequence_mut": "A",
            "comment": "Loss of interaction with ATG8 and ATG12 and no more formation of ATG12-ATG5 conjugate. Defect in Cvt pathway and autophagy.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P38862",
                    "url": "https://www.uniprot.org/uniprot/P38862"
                },
                {
                    "database": "PubMed",
                    "id": "29295865",
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                },
                {
                    "database": "PubMed",
                    "id": "10233150",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10233150"
                },
                {
                    "database": "PubMed",
                    "id": "11100732",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/11100732"
                },
                {
                    "database": "PubMed",
                    "id": "15277523",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/15277523"
                }
            ],
            "uniprot_canonical_ac": "P38862-1"
        },
        {
            "start_pos": 507,
            "end_pos": 507,
            "sequence_org": "C",
            "sequence_mut": "S",
            "comment": "Instead of the formation of an intermediate complex with a thiol ester bond between ATG7 (E1-like enzyme) and ATG8 (substrate) or between ATG7 and ATG12 (substrate), a stable complex with an O-ester bond is formed. No more formation of ATG12-ATG5 conjugate.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P38862",
                    "url": "https://www.uniprot.org/uniprot/P38862"
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                {
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                {
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                },
                {
                    "database": "PubMed",
                    "id": "15277523",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/15277523"
                }
            ],
            "uniprot_canonical_ac": "P38862-1"
        }
    ],
    "record_id": "pubmed.10233150",
    "abstract": "",
    "glycosylation": [],
    "snv": [],
    "phosphorylation": [],
    "glycation": [],
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