{
    "title": "Dual specificity protein kinase activity of testis-specific protein kinase 1 and its regulation by autophosphorylation of serine-215 within the activation loop.",
    "journal": "The Journal of biological chemistry",
    "date": "1999",
    "authors": "Toshima J, Tanaka T, Mizuno K",
    "reference": {
        "type": "PubMed",
        "id": "10207045",
        "url": "https://pubmed.ncbi.nlm.nih.gov/10207045"
    },
    "referenced_glycans": [],
    "referenced_proteins": [
        {
            "uniprot_canonical_ac": "Q15569-1",
            "protein_name": "Dual specificity testis-specific protein kinase 1",
            "gene_name": "TESK1",
            "tax_name": "Human"
        },
        {
            "uniprot_canonical_ac": "Q63572-1",
            "protein_name": "Dual specificity testis-specific protein kinase 1",
            "gene_name": "Tesk1",
            "tax_name": "Rat"
        }
    ],
    "species": [
        {
            "name": "Homo sapiens",
            "taxid": 9606,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q15569",
                    "url": "https://www.uniprot.org/uniprot/Q15569"
                }
            ],
            "common_name": "Human",
            "glygen_name": "Human",
            "reference_species": "Homo sapiens [9606]"
        }
    ],
    "phosphorylation": [
        {
            "start_pos": 220,
            "end_pos": 220,
            "kinase_uniprot_canonical_ac": "Q15569-1",
            "kinase_gene_name": "TESK1",
            "residue": "Ser",
            "comment": "",
            "evidence": [
                {
                    "database": "iPTMnet",
                    "id": "Q15569",
                    "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q15569"
                },
                {
                    "database": "PubMed",
                    "id": "10207045",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10207045"
                }
            ],
            "site_lbl": "Ser220",
            "uniprot_canonical_ac": "Q15569-1"
        },
        {
            "start_pos": 220,
            "end_pos": 220,
            "kinase_uniprot_canonical_ac": "",
            "kinase_gene_name": "",
            "residue": "Ser",
            "comment": "Phosphoserine; by autocatalysis",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q15569",
                    "url": "https://www.uniprot.org/uniprot/Q15569"
                },
                {
                    "database": "phospho.ELM",
                    "id": "Q15569",
                    "url": "http://phospho.elm.eu.org/byAccession/Q15569.html"
                },
                {
                    "database": "iPTMnet",
                    "id": "Q15569",
                    "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q15569"
                },
                {
                    "database": "PubMed",
                    "id": "10207045",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10207045"
                }
            ],
            "site_lbl": "Ser220",
            "uniprot_canonical_ac": "Q15569-1"
        },
        {
            "start_pos": 215,
            "end_pos": 215,
            "kinase_uniprot_canonical_ac": "Q63572-1",
            "kinase_gene_name": "Tesk1",
            "residue": "Ser",
            "comment": "",
            "evidence": [
                {
                    "database": "iPTMnet",
                    "id": "Q63572",
                    "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q63572"
                },
                {
                    "database": "PubMed",
                    "id": "10207045",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10207045"
                },
                {
                    "database": "UniProtKB",
                    "id": "Q63572",
                    "url": "https://www.uniprot.org/uniprot/Q63572"
                }
            ],
            "site_lbl": "Ser215",
            "uniprot_canonical_ac": "Q63572-1"
        }
    ],
    "mutagenesis": [
        {
            "start_pos": 170,
            "end_pos": 170,
            "sequence_org": "D",
            "sequence_mut": "A",
            "comment": "Loss of kinase and autophosphorylating activity. Abolishes TESK1-induced actin stress fiber formation. No effect on interaction with YWHAB, SPRY2 or SPRED1. No effect on TESK1-mediated dephosphorylation of SPRY2.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q63572",
                    "url": "https://www.uniprot.org/uniprot/Q63572"
                },
                {
                    "database": "PubMed",
                    "id": "10207045",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10207045"
                },
                {
                    "database": "PubMed",
                    "id": "17974561",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/17974561"
                },
                {
                    "database": "PubMed",
                    "id": "11555644",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/11555644"
                },
                {
                    "database": "PubMed",
                    "id": "18216281",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/18216281"
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            ],
            "uniprot_canonical_ac": "Q63572-1"
        },
        {
            "start_pos": 201,
            "end_pos": 201,
            "sequence_org": "Y",
            "sequence_mut": "A",
            "comment": "No effect on autophosphorylation.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q63572",
                    "url": "https://www.uniprot.org/uniprot/Q63572"
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            "uniprot_canonical_ac": "Q63572-1"
        },
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            "start_pos": 215,
            "end_pos": 215,
            "sequence_org": "S",
            "sequence_mut": "A",
            "comment": "Loss of autophosphorylation site, loss of kinase activity.",
            "evidence": [
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                    "id": "Q63572",
                    "url": "https://www.uniprot.org/uniprot/Q63572"
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        },
        {
            "start_pos": 215,
            "end_pos": 215,
            "sequence_org": "S",
            "sequence_mut": "E",
            "comment": "Loss of autophosphorylation site, no effect on kinase activity.",
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                    "database": "UniProtKB",
                    "id": "Q63572",
                    "url": "https://www.uniprot.org/uniprot/Q63572"
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            "start_pos": 217,
            "end_pos": 217,
            "sequence_org": "Y",
            "sequence_mut": "A",
            "comment": "No effect on autophosphorylation.",
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    "abstract": "",
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    "snv": [],
    "glycation": [],
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