{
    "title": "Systematic mutations of highly conserved His49 and carboxyl-terminal of recombinant porcine liver NADH-cytochrome b5 reductase solubilized domain.",
    "journal": "Biochimica et biophysica acta",
    "date": "1999-03-19",
    "authors": "Kimura S, Emi Y, Ikushiro S, Iyanagi T",
    "reference": {
        "type": "PubMed",
        "id": "10082957",
        "url": "https://pubmed.ncbi.nlm.nih.gov/10082957"
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    "referenced_glycans": [],
    "referenced_proteins": [
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            "uniprot_canonical_ac": "P83686-1",
            "protein_name": "NADH-cytochrome b5 reductase 3",
            "gene_name": "CYB5R3",
            "tax_name": "Pig"
        }
    ],
    "species": [
        {
            "name": "Sus scrofa",
            "taxid": 9823,
            "evidence": [
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                    "id": "P83686",
                    "url": "https://www.uniprot.org/uniprot/P83686"
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            "common_name": "Pig",
            "glygen_name": "Pig",
            "reference_species": "Sus scrofa [9823]"
        }
    ],
    "mutagenesis": [
        {
            "start_pos": 272,
            "end_pos": 272,
            "sequence_org": "F",
            "sequence_mut": "FG",
            "comment": "10% of wild-type kcat values.",
            "evidence": [
                {
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                    "id": "P83686",
                    "url": "https://www.uniprot.org/uniprot/P83686"
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        },
        {
            "start_pos": 272,
            "end_pos": 272,
            "sequence_org": "F",
            "sequence_mut": "",
            "comment": "54% of wild-type kcat values.",
            "evidence": [
                {
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                    "id": "P83686",
                    "url": "https://www.uniprot.org/uniprot/P83686"
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            "uniprot_canonical_ac": "P83686-1"
        },
        {
            "start_pos": 49,
            "end_pos": 49,
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        },
        {
            "start_pos": 49,
            "end_pos": 49,
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            "sequence_mut": "E",
            "comment": "Increase and decrease in Km values for Fe(II)-[cytochrome b5 and NADH respectively.",
            "evidence": [
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        },
        {
            "start_pos": 49,
            "end_pos": 49,
            "sequence_org": "H",
            "sequence_mut": "K",
            "comment": "Decrease and increase in Km values for Fe(II)-[cytochrome b5 and NADH respectively.",
            "evidence": [
                {
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                    "id": "P83686",
                    "url": "https://www.uniprot.org/uniprot/P83686"
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                    "id": "10082957",
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            "start_pos": 49,
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            "sequence_org": "H",
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            "comment": "Similar properties to wild-type.",
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    "abstract": "",
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    "snv": [],
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