{
    "title": "Conserved bipartite motifs in yeast eIF5 and eIF2Bepsilon, GTPase-activating and GDP-GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2.",
    "journal": "The EMBO journal",
    "date": "1999-03-15",
    "authors": "Asano K, Krishnamoorthy T, Phan L, Pavitt G D, Hinnebusch A G",
    "reference": {
        "type": "PubMed",
        "id": "10075937",
        "url": "https://pubmed.ncbi.nlm.nih.gov/10075937"
    },
    "referenced_glycans": [],
    "referenced_proteins": [
        {
            "uniprot_canonical_ac": "P38431-1",
            "protein_name": "Eukaryotic translation initiation factor 5",
            "gene_name": "TIF5",
            "tax_name": "Baker's yeast"
        },
        {
            "uniprot_canonical_ac": "P32497-1",
            "protein_name": "Eukaryotic translation initiation factor 3 subunit C",
            "gene_name": "NIP1",
            "tax_name": "Baker's yeast"
        },
        {
            "uniprot_canonical_ac": "P32501-1",
            "protein_name": "Translation initiation factor eIF2B subunit epsilon",
            "gene_name": "GCD6",
            "tax_name": "Baker's yeast"
        },
        {
            "uniprot_canonical_ac": "P09064-1",
            "protein_name": "Eukaryotic translation initiation factor 2 subunit beta",
            "gene_name": "SUI3",
            "tax_name": "Baker's yeast"
        }
    ],
    "species": [
        {
            "name": "Saccharomyces cerevisiae S288C",
            "taxid": 559292,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P38431",
                    "url": "https://www.uniprot.org/uniprot/P38431"
                }
            ],
            "common_name": "Baker's yeast",
            "glygen_name": "Yeast",
            "reference_species": "Saccharomyces cerevisiae S288C [559292]"
        }
    ],
    "mutagenesis": [
        {
            "start_pos": 388,
            "end_pos": 398,
            "sequence_org": "FITWLETAESD",
            "sequence_mut": "AATAAETAAAA",
            "comment": "In TIF5-7A; Abolishes binding to SUI3 and NIP1.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P38431",
                    "url": "https://www.uniprot.org/uniprot/P38431"
                },
                {
                    "database": "PubMed",
                    "id": "10075937",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10075937"
                }
            ],
            "uniprot_canonical_ac": "P38431-1"
        },
        {
            "start_pos": 655,
            "end_pos": 677,
            "sequence_org": "LFSALVSLYDNDIIEEDVIYKWW",
            "sequence_mut": "AFSAAVSAADNDAAEAAVAAKWA",
            "comment": "Abolishes binding to SUI3.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P32501",
                    "url": "https://www.uniprot.org/uniprot/P32501"
                },
                {
                    "database": "PubMed",
                    "id": "10075937",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10075937"
                }
            ],
            "uniprot_canonical_ac": "P32501-1"
        },
        {
            "start_pos": 696,
            "end_pos": 706,
            "sequence_org": "WVEWLQNADEE",
            "sequence_mut": "AAEAAQNAAAA",
            "comment": "Abolishes binding to SUI3; probably impairs the conversion of eIF-2-GDP to eIF-2-GTP.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P32501",
                    "url": "https://www.uniprot.org/uniprot/P32501"
                },
                {
                    "database": "PubMed",
                    "id": "10075937",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10075937"
                }
            ],
            "uniprot_canonical_ac": "P32501-1"
        },
        {
            "start_pos": 16,
            "end_pos": 23,
            "sequence_org": "KKKKKTKK",
            "sequence_mut": "AAAAATAA",
            "comment": "Abolishes interaction with TIF5; when associated with 49-K--K-56 and 82-K--K-89.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P09064",
                    "url": "https://www.uniprot.org/uniprot/P09064"
                },
                {
                    "database": "PubMed",
                    "id": "10075937",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10075937"
                }
            ],
            "uniprot_canonical_ac": "P09064-1"
        },
        {
            "start_pos": 49,
            "end_pos": 56,
            "sequence_org": "KKKKKKSK",
            "sequence_mut": "AAAAAASA",
            "comment": "Abolishes interaction with TIF5; when associated with 16-K--K-23 and 82-K--K-89.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P09064",
                    "url": "https://www.uniprot.org/uniprot/P09064"
                },
                {
                    "database": "PubMed",
                    "id": "10075937",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10075937"
                }
            ],
            "uniprot_canonical_ac": "P09064-1"
        },
        {
            "start_pos": 82,
            "end_pos": 89,
            "sequence_org": "KKKKKKTK",
            "sequence_mut": "AAAAAATA",
            "comment": "Abolishes interaction with TIF5; when associated with 16-K--K-23 and 49-K--K-56.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P09064",
                    "url": "https://www.uniprot.org/uniprot/P09064"
                },
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                    "database": "PubMed",
                    "id": "10075937",
                    "url": "https://pubmed.ncbi.nlm.nih.gov/10075937"
                }
            ],
            "uniprot_canonical_ac": "P09064-1"
        }
    ],
    "record_id": "pubmed.10075937",
    "abstract": "",
    "glycosylation": [],
    "snv": [],
    "phosphorylation": [],
    "glycation": [],
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