{ "title": "Site-specific glycan analysis of the SARS-CoV-2 spike", "journal": "Science", "date": "2020", "authors": "Watanabe Y., Allen J.D., Wrapp D., McLellan J.S., Crispin M.", "reference": { "type": "DOI", "id": "10.1126/science.abb9983", "url": "https://doi.org/10.1126/science.abb9983" }, "referenced_glycans": [ { "glytoucan_ac": "G45504EY" }, { "glytoucan_ac": "G62765YT" }, { "glytoucan_ac": "G93526NJ" }, { "glytoucan_ac": "G61613II" }, { "glytoucan_ac": "G86752LQ" }, { "glytoucan_ac": "G39446WN" }, { "glytoucan_ac": "G02886BB" }, { "glytoucan_ac": "G37399XV" }, { "glytoucan_ac": "G34852SB" }, { "glytoucan_ac": "G20956ZV" }, { "glytoucan_ac": "G49906RN" }, { "glytoucan_ac": "G28681TP" }, { "glytoucan_ac": "G66933CM" }, { "glytoucan_ac": "G20698EO" }, { "glytoucan_ac": "G73686WG" }, { "glytoucan_ac": "G87051GH" }, { "glytoucan_ac": "G00912UN" }, { "glytoucan_ac": "G80475RE" }, { "glytoucan_ac": "G44753VC" }, { "glytoucan_ac": "G41044JW" }, { "glytoucan_ac": "G85740DB" }, { "glytoucan_ac": "G36670VW" }, { "glytoucan_ac": "G82364UA" }, { "glytoucan_ac": "G60923RB" }, { "glytoucan_ac": "G58802FE" }, { "glytoucan_ac": "G72787SB" }, { "glytoucan_ac": "G50073PQ" }, { "glytoucan_ac": "G95835XS" }, { "glytoucan_ac": "G04784US" }, { "glytoucan_ac": "G43669FQ" }, { "glytoucan_ac": "G32926LW" }, { "glytoucan_ac": "G94917XT" }, { "glytoucan_ac": "G01650EU" }, { "glytoucan_ac": "G70087PV" }, { "glytoucan_ac": "G37412TK" }, { "glytoucan_ac": "G54417MJ" }, { "glytoucan_ac": "G15486FH" }, { "glytoucan_ac": "G81128KB" }, { "glytoucan_ac": "G41247ZX" }, { "glytoucan_ac": "G10339FR" }, { "glytoucan_ac": "G80735OA" }, { "glytoucan_ac": "G15038BD" }, { "glytoucan_ac": "G61855PQ" }, { "glytoucan_ac": "G37818NZ" }, { "glytoucan_ac": "G68318VE" }, { "glytoucan_ac": "G29651HS" }, { "glytoucan_ac": "G08293MJ" }, { "glytoucan_ac": "G98596OT" }, { "glytoucan_ac": "G23505EP" }, { "glytoucan_ac": "G55382TU" }, { "glytoucan_ac": "G49955PK" }, { "glytoucan_ac": "G12580WI" }, { "glytoucan_ac": "G06853GH" }, { "glytoucan_ac": "G67367OT" }, { "glytoucan_ac": "G19517GM" }, { "glytoucan_ac": "G07799LX" }, { "glytoucan_ac": "G80920RR" }, { "glytoucan_ac": "G35029YA" }, { "glytoucan_ac": "G03596YS" }, { "glytoucan_ac": "G88725PI" }, { "glytoucan_ac": "G56610MH" }, { "glytoucan_ac": "G25987BV" }, { "glytoucan_ac": "G21976AG" }, { "glytoucan_ac": "G85144OK" }, { "glytoucan_ac": "G02815KT" }, { "glytoucan_ac": "G49739MP" }, { "glytoucan_ac": "G92050GC" }, { "glytoucan_ac": "G05049IC" }, { "glytoucan_ac": "G16175ZV" }, { "glytoucan_ac": "G31852PQ" }, { "glytoucan_ac": "G59937CP" }, { "glytoucan_ac": "G69107AL" }, { "glytoucan_ac": "G09197ZW" }, { "glytoucan_ac": "G72791KH" }, { "glytoucan_ac": "G62792OG" }, { "glytoucan_ac": "G10486CT" }, { "glytoucan_ac": "G28541PG" }, { "glytoucan_ac": "G33556XM" }, { "glytoucan_ac": "G83555HU" }, { "glytoucan_ac": "G27947YN" }, { "glytoucan_ac": "G34029GR" }, { "glytoucan_ac": "G44211QA" }, { "glytoucan_ac": "G54600FO" }, { "glytoucan_ac": "G19379ID" }, { "glytoucan_ac": "G11115RO" }, { "glytoucan_ac": "G43769HG" }, { "glytoucan_ac": "G69364JQ" }, { "glytoucan_ac": "G40926MX" }, { "glytoucan_ac": "G25079LO" }, { "glytoucan_ac": "G59540CB" }, { "glytoucan_ac": "G09528DL" }, { "glytoucan_ac": "G88374WZ" }, { "glytoucan_ac": "G47737VJ" }, { "glytoucan_ac": "G44953PJ" }, { "glytoucan_ac": "G03574QJ" }, { "glytoucan_ac": "G43638QT" }, { "glytoucan_ac": "G59626AS" } ], "referenced_proteins": [ { "uniprot_canonical_ac": "P0DTC2-1", "protein_name": "Spike glycoprotein", "gene_name": "S", "tax_name": "2019-nCoV" } ], "species": [ { "name": "Severe acute respiratory syndrome coronavirus 2", "taxid": 2697049, "evidence": [ { "database": "UniProtKB", "id": "P0DTC2", "url": "https://www.uniprot.org/uniprot/P0DTC2" } ], "common_name": "2019-nCoV", "glygen_name": "SARS-CoV-2", "reference_species": "Severe acute respiratory syndrome coronavirus 2 [2697049]" } ], "glycosylation": [ { "glytoucan_ac": "G01650EU", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. 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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100100000.00010000111110.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G02815KT", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32363391", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32363391" }, { "id": "10.1101/2020.04.01.020966", "database": "DOI", "url": "https://doi.org/10.1101/2020.04.01.020966" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.10010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G08293MJ", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32363391", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32363391" }, { "id": "10.1101/2020.04.01.020966", "database": "DOI", "url": "https://doi.org/10.1101/2020.04.01.020966" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0010000.1110100000.01010000111110.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G09528DL", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 2", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0010000.1110100000.01010000111110.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G10339FR", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010001111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G15486FH", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G16175ZV", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 2", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0010000.1110000000.00000000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G19379ID", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 2", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G25987BV", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010101111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G28541PG", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.00010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G28681TP", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-T-before-R1", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.1000000.1100000000.00010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G31852PQ", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32363391", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32363391" }, { "id": "10.1101/2020.04.01.020966", "database": "DOI", "url": "https://doi.org/10.1101/2020.04.01.020966" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.10010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G37399XV", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100100000.00010000110010.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G39446WN", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G49955PK", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1100000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G50073PQ", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 2", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.00000000110110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G54600FO", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 2", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32363391", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32363391" }, { "id": "10.1101/2020.04.01.020966", "database": "DOI", "url": "https://doi.org/10.1101/2020.04.01.020966" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110100000.01010000111110.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G59626AS", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100100000.00010000111110.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G59937CP", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32363391", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32363391" }, { "id": "10.1101/2020.04.01.020966", "database": "DOI", "url": "https://doi.org/10.1101/2020.04.01.020966" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G68318VE", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G72787SB", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 122, "start_aa": "Asn", "end_pos": 122, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn122", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1100000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G72791KH", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. 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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 603, "start_aa": "Asn", "end_pos": 603, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn603", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.10010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G08293MJ", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 1", "start_pos": 603, "start_aa": "Asn", "end_pos": 603, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn603", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0010000.1110100000.01010000111110.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G10339FR", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 603, "start_aa": "Asn", "end_pos": 603, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn603", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010001111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G15486FH", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 603, "start_aa": "Asn", "end_pos": 603, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn603", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G25987BV", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 603, "start_aa": "Asn", "end_pos": 603, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn603", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010101111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G28541PG", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 603, "start_aa": "Asn", "end_pos": 603, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn603", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.00010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G39446WN", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 603, "start_aa": "Asn", "end_pos": 603, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn603", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G41247ZX", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 603, "start_aa": "Asn", "end_pos": 603, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn603", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.10010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G59937CP", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 603, "start_aa": "Asn", "end_pos": 603, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn603", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": 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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 616, "start_aa": "Asn", "end_pos": 616, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn616", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010001111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G10486CT", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. 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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 61, "start_aa": "Asn", "end_pos": 61, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn61", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.00010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G15486FH", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 61, "start_aa": "Asn", "end_pos": 61, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn61", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G25987BV", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides before enrichment (trypsin digestion)", "start_pos": 61, "start_aa": "Asn", "end_pos": 61, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn61", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010101111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G28541PG", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 61, "start_aa": "Asn", "end_pos": 61, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn61", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.00010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G29651HS", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 2", "start_pos": 61, "start_aa": "Asn", "end_pos": 61, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn61", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0010000.1110000000.00010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G31852PQ", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-T-after-R1", "start_pos": 61, "start_aa": "Asn", "end_pos": 61, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn61", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.10010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G35029YA", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 61, "start_aa": "Asn", "end_pos": 61, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn61", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1100000000.01010001111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G37399XV", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 61, "start_aa": "Asn", "end_pos": 61, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn61", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100100000.00010000110010.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G39446WN", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 61, "start_aa": "Asn", "end_pos": 61, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn61", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://doi.org/10.1101/2020.03.28.013276" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G41247ZX", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. 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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G62765YT", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.10010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G88725PI", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G92050GC", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-G-after-R1", "start_pos": 709, "start_aa": "Asn", "end_pos": 709, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.00010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G01650EU", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100100000.00010000111110.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G02815KT", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.10010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G03574QJ", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 1", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1110000000.01010000110110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G08293MJ", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 3", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0010000.1110100000.01010000111110.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G09528DL", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 1", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0010000.1110100000.01010000111110.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G10339FR", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010001111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G10486CT", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.00010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G15486FH", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G28541PG", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.00010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G31852PQ", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.10010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G34029GR", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 3", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0010000.1100000000.01010000110010.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G35029YA", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 1", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1100000000.01010001111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G37399XV", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100100000.00010000110010.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G39446WN", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G41247ZX", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.10010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G50073PQ", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 3", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.00000000110110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G54600FO", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 1", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110100000.01010000111110.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G58802FE", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 3", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0010000.1110000000.00000000110010.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G59937CP", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G80475RE", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 1", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01000101111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G80920RR", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 1", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32518941" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.10010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G83555HU", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G92050GC", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 717, "start_aa": "Asn", "end_pos": 717, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32518941", "database": 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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.10010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G08293MJ", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32363391", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32363391" }, { "id": "10.1101/2020.04.01.020966", "database": "DOI", "url": "https://doi.org/10.1101/2020.04.01.020966" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0010000.1110100000.01010000111110.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G10486CT", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.00010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G15486FH", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G25987BV", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32363391", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32363391" }, { "id": "10.1101/2020.04.01.020966", "database": "DOI", "url": "https://doi.org/10.1101/2020.04.01.020966" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010101111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G28541PG", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.00010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G28681TP", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.1000000.1100000000.00010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G31852PQ", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.10010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G35029YA", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1100000000.01010001111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G37399XV", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100100000.00010000110010.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G39446WN", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G41247ZX", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.10010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G45504EY", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1100100000.00010000110110.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G50073PQ", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 1", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.00000000110110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G54600FO", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 2", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32363391", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32363391" }, { "id": "10.1101/2020.04.01.020966", "database": "DOI", "url": "https://doi.org/10.1101/2020.04.01.020966" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110100000.01010000111110.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G59937CP", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "32363391", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32363391" }, { "id": "10.1101/2020.04.01.020966", "database": "DOI", "url": "https://doi.org/10.1101/2020.04.01.020966" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G72787SB", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1100000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G72791KH", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 3", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1110100000.01010000111010.00100.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G80475RE", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-T-before-R1", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01000101111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G88725PI", "type": "N-linked;o-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00000000001000.0100000.1110000000.01010000111110.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" }, { "glytoucan_ac": "G92050GC", "type": "N-linked", "subtype": "other", "prediction_tool": "", "mining_tool": "", "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "start_pos": 801, "start_aa": "Asn", "end_pos": 801, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://doi.org/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://doi.org/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://doi.org/10.1126/science.abb9983" }, { "id": "33064451", "database": "PubMed", "url": "https://pubmed.ncbi.nlm.nih.gov/33064451" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://doi.org/10.1021/acs.analchem.0c03301" } ], "filter_code": "00100000000000000000.0000000.00010000000000.0100000.1100000000.00010101111111.00010.01000", "uniprot_canonical_ac": "P0DTC2-1" } ], "record_id": "doi.10.1126/science.abb9983", "abstract": "", "mutagenesis": [], "snv": [], "phosphorylation": [], "glycation": [], "section_stats": [ { "table_id": "referenced_proteins", "table_stats": [ { "field": "total", "count": 1 } ], "sort_fields": [ "uniprot_canonical_ac", "protein_name", "gene_name", "tax_name" ] }, { "table_id": "referenced_glycans", "table_stats": [ { "field": "total", "count": 97 } ], "sort_fields": [ "glytoucan_ac" ] }, { "table_id": "glycosylation", "table_stats": [ { "field": "total", "count": 561 }, { "field": "total_sites", "count": 22 }, { "field": "n_linked_glycans", "count": 71 }, { "field": "n_linked_glycan_sites", "count": 24 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [ "glytoucan_ac", "type", "subtype", "prediction_tool", "mining_tool", "site_category", "site_seq", "relation", "comment", "start_pos", "start_aa", "end_pos", "end_aa", "residue", "site_lbl", "filter_code", "uniprot_canonical_ac" ] }, { "table_id": "phosphorylation", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_reported", "table_stats": [ { "field": "total", "count": 3 }, { "field": "total_sites", "count": 3 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 3 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [ "glytoucan_ac", "type", "subtype", "prediction_tool", "mining_tool", "site_category", "site_seq", "relation", "comment", "start_pos", "start_aa", "end_pos", "end_aa", "residue", "site_lbl", "filter_code", "uniprot_canonical_ac" ] }, { "table_id": "glycosylation_reported_with_glycan", "table_stats": [ { "field": "total", "count": 558 }, { "field": "total_sites", "count": 22 }, { "field": "n_linked_glycans", "count": 71 }, { "field": "n_linked_glycan_sites", "count": 21 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [ "glytoucan_ac", "type", "subtype", "prediction_tool", "mining_tool", "site_category", "site_seq", "relation", "comment", "start_pos", "start_aa", "end_pos", "end_aa", "residue", "site_lbl", "filter_code", "uniprot_canonical_ac" ] }, { "table_id": "glycosylation_predicted", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_automatic_literature_mining", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [] } ] }