{ "uniprot_canonical_ac": "P0CD61-1", "uniprot_ac": "P0CD61", "uniprot_id": "GPT_DICDI", "mass": { "chemical_mass": 45452.0, "monoisotopic_mass": 45452.0 }, "refseq": {}, "gene_names": [ { "name": "alg7", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/P0CD61", "id": "P0CD61" }, { "name": "DDB_G0290751", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/P0CD61", "id": "P0CD61" } ], "protein_names": [ { "name": "UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase", "resource": "UniProtKB", "type": "recommended", "url": "", "id": "P0CD61" }, { "name": "2.7.8.15", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "P0CD61" }, { "name": "G1PT", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "P0CD61" }, { "name": "GPT", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "P0CD61" }, { "name": "GlcNAc-1-P transferase", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "P0CD61" }, { "name": "N-acetylglucosamine-1-phosphate transferase", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "P0CD61" } ], "function": [ { "annotation": "Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis in N-linked protein glycosylation pathway: transfers GlcNAc-1-P from UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol.", "evidence": [ { "database": "UniProtKB", "id": "P0CD61", "url": "https://www.uniprot.org/uniprot/P0CD61#function" }, { "database": "UniProtKB", "id": "Q9H3H5", "url": "https://www.uniprot.org/uniprot/Q9H3H5#function" } ] } ], "disease": [], "snv": [], "mutagenesis": [], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Dictyostelium discoideum", "taxid": 44689, "evidence": [ { "database": "UniProtKB", "id": "P0CD61", "url": "https://www.uniprot.org/uniprot/P0CD61" } ], "common_name": "Cellular slime molds" } ], "gene": [ { "name": "alg7", "url": "https://www.uniprot.org/uniprot/P0CD61", "locus": {} } ], "crossref": [ { "id": "cd06855", "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd06855", "database": "CDD", "categories": [ "Protein Classification" ] }, { "id": "DDB_G0290751", "url": "http://dictybase.org/gene/DDB_G0290751", "database": "dictyBase", "categories": [ "Organism-specific" ] }, { "id": "IPR000715", "url": "https://www.ebi.ac.uk/interpro/entry/IPR000715", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR033895", "url": "https://www.ebi.ac.uk/interpro/entry/IPR033895", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "LPHFNAR", "url": "https://omabrowser.org/oma/group/LPHFNAR/", "database": "OMA", "categories": [ "Protein Classification" ] }, { "id": "PTHR10571", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR10571", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PTHR10571:SF0", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR10571:SF0", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PF00953", "url": "http://pfam.xfam.org/family/PF00953", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PR_P0CD61", "url": "http://purl.obolibrary.org/obo/PR_P0CD61", "database": "PRO", "categories": [ "Protein" ] }, { "id": "R-DDI-446193", "url": "https://www.reactome.org/PathwayBrowser/#R-DDI-446193", "database": "Reactome", "categories": [ "Protein Classification" ] }, { "id": "13289", "url": "https://www.rhea-db.org/rhea/13289", "database": "Rhea", "categories": [ "Enzyme and Pathway" ] }, { "id": "P0CD61", "url": "https://www.uniprot.org/uniprot/P0CD61", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "P0CD61-1", "url": "https://www.uniprot.org/uniprot/P0CD61-1", "database": "UniProtKB", "categories": [ "Protein" ] } ], "sequence": { "sequence": "MIFDKSLLGFIGICCLPPILLLIYLPNVSLQWIIVQSGFFSFGAGLLTYKLIPSVADLTSQANLTGMDLNKKGDPKFSGKKIPESLGICVAVVYLVCVILFQTFQWFSFPETIQLSEYNAALTSICFMILLGFGDDVLNLRWRYKLILPMFASLPLLVAYAGGTTVVVPDINFPVPLREWLGVVFDLGIFYRIYLLMLAIFCTNSINILAGINGLEVGQSVVIATSIIIHNLIELTIASSVPSSSSFSSSALSSVSPHLFSLILMIPFLFTTISLLFYNWYPSRVFVGDTYTYFSGMCFAVVAILCHFSKTLLLFFIPQILNFLYSVPQLFGIIPCPRHRVPKFNPETGKMEAIPTNLTIINLLLMITGPLTERQLCVYLLIFQGLCSCVGFGIRYHHLHHNPTLIFI", "length": 408, "header": "sp|P0CD61-1|GPT_DICDI Isoform 1 of UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase OS=Dictyostelium discoideum OX=44689 GN=alg7 PE=3 SV=1 CANONICAL=P0CD61-1" }, "isoforms": [ { "isoform_ac": "P0CD61-1", "url": "", "sequence": { "sequence": "MIFDKSLLGFIGICCLPPILLLIYLPNVSLQWIIVQSGFFSFGAGLLTYKLIPSVADLTSQANLTGMDLNKKGDPKFSGKKIPESLGICVAVVYLVCVILFQTFQWFSFPETIQLSEYNAALTSICFMILLGFGDDVLNLRWRYKLILPMFASLPLLVAYAGGTTVVVPDINFPVPLREWLGVVFDLGIFYRIYLLMLAIFCTNSINILAGINGLEVGQSVVIATSIIIHNLIELTIASSVPSSSSFSSSALSSVSPHLFSLILMIPFLFTTISLLFYNWYPSRVFVGDTYTYFSGMCFAVVAILCHFSKTLLLFFIPQILNFLYSVPQLFGIIPCPRHRVPKFNPETGKMEAIPTNLTIINLLLMITGPLTERQLCVYLLIFQGLCSCVGFGIRYHHLHHNPTLIFI", "length": 408, "header": "sp|P0CD61-1|GPT_DICDI Isoform 1 of UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase OS=Dictyostelium discoideum OX=44689 GN=alg7 PE=3 SV=1 CANONICAL=P0CD61-1" }, "locus": {} } ], "glycosylation": [], "phosphorylation": [], "glycation": [], "keywords": [ "enzyme" ], "publication": [ { "title": "The genome of the social amoeba Dictyostelium discoideum.", "journal": "Nature", "date": "2005", "authors": "Eichinger L, Pachebat J A, Gl\u00f6ckner G, Rajandream M-A, Sucgang R, Berriman M, Song J, Olsen R, Szafranski K, Xu Q, Tunggal B, Kummerfeld S, Madera M, Konfortov B A, Rivero F, Bankier A T, Lehmann R, Hamlin N, Davies R, Gaudet P, Fey P, Pilcher K, Chen G, Saunders D, Sodergren E, Davis P, Kerhornou A, Nie X, Hall N, Anjard C, Hemphill L, Bason N, Farbrother P, Desany B, Just E, Morio T, Rost R, Churcher C, Cooper J, Haydock S, van Driessche N, Cronin A, Goodhead I, Muzny D, Mourier T, Pain A, Lu M, Harper D, Lindsay R, Hauser H, James K, Quiles M, Madan Babu M, Saito T, Buchrieser C, Wardroper A, Felder M, Thangavelu M, Johnson D, Knights A, Loulseged H, Mungall K, Oliver K, Price C, Quail M A, Urushihara H, Hernandez J, Rabbinowitsch E, Steffen D, Sanders M, Ma J, Kohara Y, Sharp S, Simmonds M, Spiegler S, Tivey A, Sugano S, White B, Walker D, Woodward J, Winckler T, Tanaka Y, Shaulsky G, Schleicher M, Weinstock G, Rosenthal A, Cox E C, Chisholm R L, Gibbs R, Loomis W F, Platzer M, Kay R R, Williams J, Dear P H, Noegel A A, Barrell B, Kuspa A", "evidence": [ { "database": "UniProtKB", "id": "P0CD61", "url": "https://www.uniprot.org/uniprot/P0CD61" } ], "reference": [ { "type": "PubMed", "id": "15875012", "url": "https://glygen.org/publication/PubMed/15875012" } ] } ], "pathway": [ { "id": "R-DDI-446193", "name": "Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein", "url": "https://www.reactome.org/PathwayBrowser/#R-DDI-446193", "resource": "Reactome", "description": "Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein" } ], "orthologs": [], "go_annotation": { "categories": [ { "name": "Molecular Function", "total": 3, "go_terms": [ { "name": "metal ion binding", "id": "GO:0046872", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046872", "evidence": [ { "database": "UniProtKB", "id": "P0CD61", "url": "https://www.uniprot.org/uniprot/P0CD61" } ], "pmid": "" }, { "name": "glycosyltransferase activity", "id": "GO:0016757", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016757", "evidence": [ { "database": "UniProtKB", "id": "P0CD61", "url": "https://www.uniprot.org/uniprot/P0CD61" } ], "pmid": "" }, { "name": "UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity", "id": "GO:0003975", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003975", "evidence": [ { "database": "UniProtKB", "id": "P0CD61", "url": "https://www.uniprot.org/uniprot/P0CD61" } ], "pmid": "" } ] }, { "name": "Cellular Component", "total": 1, "go_terms": [ { "name": "endoplasmic reticulum membrane", "id": "GO:0005789", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005789", "evidence": [ { "database": "UniProtKB", "id": "P0CD61", "url": "https://www.uniprot.org/uniprot/P0CD61" } ], "pmid": "" } ] }, { "name": "Biological Process", "total": 1, "go_terms": [ { "name": "dolichol-linked oligosaccharide biosynthetic process", "id": "GO:0006488", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006488", "evidence": [ { "database": "UniProtKB", "id": "P0CD61", "url": "https://www.uniprot.org/uniprot/P0CD61" } ], "pmid": "" } ] } ] }, "pro_annotation": [], "site_annotation": [ { "start_pos": 27, "end_pos": 29, "annotation": "n_glycosylation_sequon" }, { "start_pos": 357, "end_pos": 359, "annotation": "n_glycosylation_sequon" }, { "start_pos": 63, "end_pos": 65, "annotation": "n_glycosylation_sequon" } ], "ptm_annotation": [], "interactions": [], "reactions": [ { "id": "13289", "name": "", "equation": "a dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = N-acetyl-alpha-D-glucosaminyl-diphosphodolichol + UMP", "summary": "", "cellular_location": "", "input_participant_list": [], "output_participant_list": [], "enzyme_list": [ "F7EMK2-1", "Q9H3H5-1", "P42867-1", "P07286-1", "P0CD61-1", "Q9VK30-1" ], "evidence": [ { "id": "13289", "database": "Rhea", "url": "https://www.rhea-db.org/rhea/13289" } ] }, { "id": "R-DDI-446191", "name": "Addition of N-acetyl-D-glucosamine to Dolichyl phosphate", "equation": "", "summary": "This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp", "cellular_location": "cytosol, endoplasmic reticulum membrane", "input_participant_list": [], "output_participant_list": [ "R-ALL-449222" ], "enzyme_list": [ "P0CD61-1" ], "evidence": [ { "id": "R-DDI-446191", "database": "Reactome", "url": "https://www.reactome.org/PathwayBrowser/#R-DDI-446191" } ] } ], "reaction_participants": [ { "participant_id": "R-ALL-449222", "participant_name": "GlcNAcDOLDP [integral component of cytoplasmic side of endoplasmic reticulum membrane]", "crossref": [ { "id": "18278", "database": "ChEBI", "url": "" }, { "id": "000087493148", "database": "ZINC", "url": "" }, { "id": "000087493147", "database": "ZINC", "url": "" }, { "id": "18278", "database": "ChEBI", "url": "" }, { "id": "000087493148", "database": "ZINC", "url": "" }, { "id": "000087493147", "database": "ZINC", "url": "" }, { "id": "18278", "database": "ChEBI", "url": "" }, { "id": "000087493148", "database": "ZINC", "url": "" }, { "id": "000087493147", "database": "ZINC", "url": "" }, { "id": "18278", "database": "ChEBI", "url": "" }, { "id": "000087493148", "database": "ZINC", "url": "" }, { "id": "000087493147", "database": "ZINC", "url": "" }, { "id": "18278", "database": "ChEBI", "url": "" }, { "id": "000087493148", "database": "ZINC", "url": "" }, { "id": "000087493147", "database": "ZINC", "url": "" }, { "id": "18278", "database": "ChEBI", "url": "" }, { "id": "000087493148", "database": "ZINC", "url": "" }, { "id": "000087493147", "database": "ZINC", "url": "" }, { "id": "000087493148", "database": "ZINC", "url": "" }, { "id": "000087493147", "database": "ZINC", "url": "" }, { "id": "18278", "database": "ChEBI", "url": "" } ] } ], "reaction_enzymes": [ { "uniprot_ac": "F7EMK2", "gene_name": "Dpagt1", "activity": [ { "ec_number": "2.7.8.15", "activity": "a dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = N-acetyl-alpha-D-glucosaminyl-diphosphodolichol + UMP.", "evidence": [ { "id": "F7EMK2", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/F7EMK2" } ] }, { "ec_number": "2.7.8.15", "activity": "", "evidence": [ { "id": "F7EMK2", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/F7EMK2" } ] } ] }, { "uniprot_ac": "P07286", "gene_name": "ALG7", "activity": [ { "ec_number": "2.7.8.15", "activity": "a dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = N-acetyl-alpha-D-glucosaminyl-diphosphodolichol + UMP.", "evidence": [ { "id": "P07286", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P07286" } ] }, { "ec_number": "2.7.8.15", "activity": "", "evidence": [ { "id": "P07286", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P07286" } ] } ] }, { "uniprot_ac": "P0CD61", "gene_name": "alg7", "activity": [ { "ec_number": "2.7.8.15", "activity": "a dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = N-acetyl-alpha-D-glucosaminyl-diphosphodolichol + UMP.", "evidence": [ { "id": "P0CD61", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P0CD61" } ] }, { "ec_number": "2.7.8.15", "activity": "", "evidence": [ { "id": "P0CD61", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P0CD61" } ] } ] }, { "uniprot_ac": "P42867", "gene_name": "Dpagt1", "activity": [ { "ec_number": "2.7.8.15", "activity": "a dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = N-acetyl-alpha-D-glucosaminyl-diphosphodolichol + UMP.", "evidence": [ { "id": "P42867", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P42867" } ] }, { "ec_number": "2.7.8.15", "activity": "", "evidence": [ { "id": "P42867", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P42867" } ] } ] }, { "uniprot_ac": "Q9H3H5", "gene_name": "DPAGT1", "activity": [ { "ec_number": "2.7.8.15", "activity": "a dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = N-acetyl-alpha-D-glucosaminyl-diphosphodolichol + UMP.", "evidence": [ { "id": "Q9H3H5", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q9H3H5" } ] }, { "ec_number": "2.7.8.15", "activity": "", "evidence": [ { "id": "Q9H3H5", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q9H3H5" } ] } ] }, { "uniprot_ac": "Q9VK30", "gene_name": "Alg7", "activity": [ { "ec_number": "2.7.8.15", "activity": "a dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = N-acetyl-alpha-D-glucosaminyl-diphosphodolichol + UMP.", "evidence": [ { "id": "Q9VK30", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q9VK30" } ] }, { "ec_number": "2.7.8.15", "activity": "", "evidence": [ { "id": "Q9VK30", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q9VK30" } ] } ] } ], "biomarkers": [], "synthesized_glycans": [], "cluster_types": [], "sequence_features": { "n_linked_sites": [], "o_linked_sites": [], "snv_sites": [], "glycation_sites": [], "phosphorylation_sites": [], "sequon_annotation_sites": [ { "start_pos": 27, "end_pos": 29 }, { "start_pos": 357, "end_pos": 359 }, { "start_pos": 63, "end_pos": 65 } ] }, "section_stats": [ { "table_id": "glycosylation", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_reported", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_reported_with_glycan", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_predicted", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_automatic_literature_mining", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "phosphorylation", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "snv", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "snv_disease", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "snv_non_disease", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "publication", "table_stats": [ { "field": "total", "count": 1 } ], "sort_fields": [ "title", "journal", "date", "authors" ] } ] }