{ "uniprot_canonical_ac": "O96759-1", "uniprot_ac": "O96759", "uniprot_id": "ADAS_DICDI", "mass": { "chemical_mass": 69408.0, "monoisotopic_mass": 69408.0 }, "refseq": { "ac": "XP_637836", "name": "alkyl-dihydroxyacetonephosphate synthase", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/XP_637836" }, "gene_names": [ { "name": "eapA", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/O96759", "id": "O96759" }, { "name": "DDB_G0286183", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/O96759", "id": "O96759" }, { "name": "agps", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/XP_637836", "id": "XP_637836" } ], "protein_names": [ { "name": "Alkyldihydroxyacetonephosphate synthase", "resource": "UniProtKB", "type": "recommended", "url": "", "id": "O96759" }, { "name": "2.5.1.26", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "O96759" }, { "name": "Alkyl-DHAP synthase", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "O96759" }, { "name": "Alkylglycerone-phosphate synthase", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "O96759" }, { "name": "alkyl-dihydroxyacetonephosphate synthase", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/XP_637836", "id": "XP_637836" }, { "name": "alkyldihydroxyacetonephosphate synthase", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/XP_637836", "id": "XP_637836" } ], "function": [ { "annotation": "Catalyzes the exchange of an acyl for a long-chain alkyl group and the formation of the ether bond in the biosynthesis of ether phospholipids.", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759#function" }, { "database": "PubMed", "id": "17562315", "url": "https://glygen.org/publication/PubMed/17562315" } ] } ], "disease": [], "snv": [], "mutagenesis": [ { "start_pos": 352, "end_pos": 352, "sequence_org": "R", "sequence_mut": "H", "comment": "30-fold reduction in activity.", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" }, { "database": "PubMed", "id": "17562315", "url": "https://glygen.org/publication/PubMed/17562315" } ] }, { "start_pos": 447, "end_pos": 447, "sequence_org": "R", "sequence_mut": "L", "comment": "Loss of activity. The FAD binds poorly to the mutant enzyme.", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" }, { "database": "PubMed", "id": "17562315", "url": "https://glygen.org/publication/PubMed/17562315" } ] }, { "start_pos": 508, "end_pos": 508, "sequence_org": "Y", "sequence_mut": "F", "comment": "Loss of activity.", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" }, { "database": "PubMed", "id": "17562315", "url": "https://glygen.org/publication/PubMed/17562315" } ] }, { "start_pos": 544, "end_pos": 544, "sequence_org": "H", "sequence_mut": "I", "comment": "Loss of activity.", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" }, { "database": "PubMed", "id": "17562315", "url": "https://glygen.org/publication/PubMed/17562315" } ] }, { "start_pos": 545, "end_pos": 545, "sequence_org": "H", "sequence_mut": "I", "comment": "Loss of activity.", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" }, { "database": "PubMed", "id": "17562315", "url": "https://glygen.org/publication/PubMed/17562315" } ] }, { "start_pos": 546, "end_pos": 546, "sequence_org": "H", "sequence_mut": "I", "comment": "Loss of activity.", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" }, { "database": "PubMed", "id": "17562315", "url": "https://glygen.org/publication/PubMed/17562315" } ] } ], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Dictyostelium discoideum", "taxid": 44689, "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" } ], "common_name": "Cellular slime molds" } ], "gene": [ { "name": "eapA", "url": "https://www.uniprot.org/uniprot/O96759", "locus": {} } ], "crossref": [ { "id": "2.5.1.26", "url": 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] }, { "id": "IPR016167", "url": "https://www.ebi.ac.uk/interpro/entry/IPR016167", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR016171", "url": "https://www.ebi.ac.uk/interpro/entry/IPR016171", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR025650", "url": "https://www.ebi.ac.uk/interpro/entry/IPR025650", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR036318", "url": "https://www.ebi.ac.uk/interpro/entry/IPR036318", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "ddi:DDB_G0286183", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+ddi:DDB_G0286183", "database": "KEGG Pathway", "categories": [ "Enzyme and Pathway" ] }, { "id": "GTISHQH", "url": "https://omabrowser.org/oma/group/GTISHQH/", "database": "OMA", "categories": [ "Protein Classification" ] }, { "id": "PTHR46568", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR46568", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PTHR46568:SF1", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR46568:SF1", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "2UUU", "url": "https://www.rcsb.org/structure/2UUU", "database": "PDB", "categories": [ "3D Structure" ] }, { "id": "2UUV", "url": "https://www.rcsb.org/structure/2UUV", "database": "PDB", "categories": [ "3D Structure" ] }, { "id": "PF01565", "url": "http://pfam.xfam.org/family/PF01565", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PF02913", "url": "http://pfam.xfam.org/family/PF02913", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PR_O96759", "url": "http://purl.obolibrary.org/obo/PR_O96759", "database": "PRO", "categories": [ "Protein" ] }, { "id": "XP_637836", "url": "https://www.ncbi.nlm.nih.gov/protein/XP_637836", "database": "RefSeq", "categories": [ "Protein" ] }, { "id": "36171", "url": "https://www.rhea-db.org/rhea/36171", "database": "Rhea", "categories": [ "Enzyme and Pathway" ] }, { "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "O96759-1", "url": "https://www.uniprot.org/uniprot/O96759-1", "database": "UniProtKB", "categories": [ "Protein" ] } ], "sequence": { "sequence": "MSGEKKEYPKEHIDLYQQIKWNGWGDTRKFLHQLKPSGTIAMTTPEVSSVPLPSLRGFIKKELTLPGEEDKPFVLDETPALQIENIHVDPPKQYPEFVRELKAFFLPDQLKDDKLARITHTFGKSLRDLIRVRIGQVKNAPDLIVLPHSHEEVERLVQLAHKYNVVIIPMGGGSNIVGAIEPVSNERFTVSIDMRRMNKVLWVDRREMTACIQVGIMGPELEKQLHKQGVSLGHDPDSFEFSTLGGWLATCSSGHQSDKYGDIEDMAVSFRTVTPTGTLELRNGARSGAGINYKHIILGSEGTLGIITEAVMKVHAVPQAVEYYGFLFPTFAHAVSALQQIRSSEVIPTMIRVYDPEETQLSFAWKPSKGAVSEFTSAMVKKYLHYIRSFDFKNVCLSIIGFEGPKKVVDFHRTSVFDILSKNAAFGLGSAPGKTWAEKRYDLPYIRDFLLDHNMWVDVAETTVSYANLQTLWKDAKQTFVKHFKDQGIPAWICAHISHTYTNGVCLYFIFASKQNENKDMAQYIEAKKLMTDIIFKYGGSLSHHHGVGYEHVPWMTRYATRGWINVYRSLKETIDPKDICNPRKLIPTIKEENNKEPFLFDVVNVKYPKL", "length": 611, "header": "sp|O96759-1|ADAS_DICDI Isoform 1 of Alkyldihydroxyacetonephosphate synthase OS=Dictyostelium discoideum OX=44689 GN=eapA PE=1 SV=1 CANONICAL=O96759-1" }, "isoforms": [ { "isoform_ac": "O96759-1", "url": "", "sequence": { "sequence": "MSGEKKEYPKEHIDLYQQIKWNGWGDTRKFLHQLKPSGTIAMTTPEVSSVPLPSLRGFIKKELTLPGEEDKPFVLDETPALQIENIHVDPPKQYPEFVRELKAFFLPDQLKDDKLARITHTFGKSLRDLIRVRIGQVKNAPDLIVLPHSHEEVERLVQLAHKYNVVIIPMGGGSNIVGAIEPVSNERFTVSIDMRRMNKVLWVDRREMTACIQVGIMGPELEKQLHKQGVSLGHDPDSFEFSTLGGWLATCSSGHQSDKYGDIEDMAVSFRTVTPTGTLELRNGARSGAGINYKHIILGSEGTLGIITEAVMKVHAVPQAVEYYGFLFPTFAHAVSALQQIRSSEVIPTMIRVYDPEETQLSFAWKPSKGAVSEFTSAMVKKYLHYIRSFDFKNVCLSIIGFEGPKKVVDFHRTSVFDILSKNAAFGLGSAPGKTWAEKRYDLPYIRDFLLDHNMWVDVAETTVSYANLQTLWKDAKQTFVKHFKDQGIPAWICAHISHTYTNGVCLYFIFASKQNENKDMAQYIEAKKLMTDIIFKYGGSLSHHHGVGYEHVPWMTRYATRGWINVYRSLKETIDPKDICNPRKLIPTIKEENNKEPFLFDVVNVKYPKL", "length": 611, "header": "sp|O96759-1|ADAS_DICDI Isoform 1 of Alkyldihydroxyacetonephosphate synthase OS=Dictyostelium discoideum OX=44689 GN=eapA PE=1 SV=1 CANONICAL=O96759-1" }, "locus": {} } ], "glycosylation": [], "phosphorylation": [], "glycation": [], "keywords": [ "enzyme" ], "publication": [ { "title": "The genome of the social amoeba Dictyostelium discoideum.", "journal": 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Chisholm R L, Gibbs R, Loomis W F, Platzer M, Kay R R, Williams J, Dear P H, Noegel A A, Barrell B, Kuspa A", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" } ], "reference": [ { "type": "PubMed", "id": "15875012", "url": "https://glygen.org/publication/PubMed/15875012" } ] }, { "title": "The crucial step in ether phospholipid biosynthesis: structural basis of a noncanonical reaction associated with a peroxisomal disorder.", "journal": "Structure (London, England : 1993)", "date": "2007-06", "authors": "Razeto Adelia, Mattiroli Francesca, Carpanelli Elena, Aliverti Alessandro, Pandini Vittorio, Coda Alessandro, Mattevi Andrea", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" } ], "reference": [ { "type": "PubMed", "id": "17562315", "url": "https://glygen.org/publication/PubMed/17562315" } ] }, { "title": "Nucleotide sequence of alkyl-dihydroxyacetonephosphate synthase cDNA from Dictyostelium discoideum.", "journal": "Biochem. Biophys. Res. Commun.", "date": "1998", "authors": "de Vet E C, van den Bosch H", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" } ], "reference": [ { "type": "PubMed", "id": "9837757", "url": "https://glygen.org/publication/PubMed/9837757" } ] } ], "pathway": [ { "id": "ddi:DDB_G0286183", "name": "", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+ddi:DDB_G0286183", "resource": "KEGG Pathway" } ], "orthologs": [], "go_annotation": { "categories": [ { "name": "Cellular Component", "total": 1, "go_terms": [ { "name": "peroxisome", "id": "GO:0005777", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005777", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" } ], "pmid": "" } ] }, { "name": "Molecular Function", "total": 5, "go_terms": [ { "name": "alkylglycerone-phosphate synthase activity", "id": "GO:0008609", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0008609", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" } ], "pmid": "" }, { "name": "identical protein binding", "id": "GO:0042802", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042802", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" } ], "pmid": "" }, { "name": "flavin adenine dinucleotide binding", "id": "GO:0050660", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050660", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" } ], "pmid": "" }, { "name": "FAD binding", "id": "GO:0071949", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0071949", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" } ], "pmid": "" }, { "name": "alcohol binding", "id": "GO:0043178", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043178", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" } ], "pmid": "" } ] }, { "name": "Biological Process", "total": 1, "go_terms": [ { "name": "ether lipid biosynthetic process", "id": "GO:0008611", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0008611", "evidence": [ { "database": "UniProtKB", "id": "O96759", "url": "https://www.uniprot.org/uniprot/O96759" } ], "pmid": "" } ] } ] }, "pro_annotation": [], "site_annotation": [], "ptm_annotation": [], "interactions": [], "reactions": [ { "id": "36171", "name": "", "equation": "a 1-acylglycerone 3-phosphate + a long chain fatty alcohol = 1-O-alkylglycerone 3-phosphate + a long-chain fatty acid + H(+)", "summary": "", "cellular_location": "", "input_participant_list": [], "output_participant_list": [], "enzyme_list": [ "Q9EQR2-1", "O00116-1", "Q8C0I1-1", "O96759-1", "Q9V778-1" ], "evidence": [ { "id": "36171", "database": "Rhea", "url": "https://www.rhea-db.org/rhea/36171" } ] } ], "reaction_participants": [], "reaction_enzymes": [ { "uniprot_ac": 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