{ "uniprot_canonical_ac": "O96612-1", "uniprot_ac": "O96612", "uniprot_id": "UBPB_DICDI", "mass": { "chemical_mass": 51134.0, "monoisotopic_mass": 51134.0 }, "refseq": { "ac": "XP_643807", "name": "deubiquinating enzyme", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/XP_643807" }, "gene_names": [ { "name": "ubpB", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/O96612", "id": "O96612" }, { "name": "DDB_G0275021", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/O96612", "id": "O96612" }, { "name": "ubpB", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/XP_643807", "id": "XP_643807" } ], "protein_names": [ { "name": "Ubiquitin hydrolase B", "resource": "UniProtKB", "type": "recommended", "url": "", "id": "O96612" }, { "name": "3.4.19.12", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "O96612" }, { "name": "deubiquinating enzyme", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/XP_643807", "id": "XP_643807" }, { "name": "peptidase C19 family protein; ubiquitin hydrolase B", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/XP_643807", "id": "XP_643807" } ], "function": [ { "annotation": "Required for proper prespore cell patterning. Plays a role in stabilizing mkkA by preventing it from being targeted for degradation. ubcB and ubpB differentially control ubiquitination/deubiquitination and degradation of mkkA in a cell-type-specific and temporally regulated manner.", "evidence": [ { "database": "UniProtKB", "id": "O96612", "url": "https://www.uniprot.org/uniprot/O96612#function" }, { "database": "PubMed", "id": "9832508", "url": "https://glygen.org/publication/PubMed/9832508" } ] } ], "disease": [], "snv": [], "mutagenesis": [], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Dictyostelium discoideum", "taxid": 44689, "evidence": [ { "database": "UniProtKB", "id": "O96612", "url": "https://www.uniprot.org/uniprot/O96612" } ], "common_name": "Cellular slime molds" } ], "gene": [ { "name": "ubpB", "url": "https://www.uniprot.org/uniprot/O96612", "locus": {} } ], "crossref": [ { "id": "cd02257", "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd02257", "database": "CDD", "categories": [ "Protein Classification" ] }, { "id": "DDB_G0275021", "url": "http://dictybase.org/gene/DDB_G0275021", "database": "dictyBase", "categories": [ "Organism-specific" ] }, { "id": "8619853", "url": "https://www.ncbi.nlm.nih.gov/gene/8619853", "database": "GeneID", "categories": [ "Genome Annotation" ] }, { "id": "IPR001394", "url": "https://www.ebi.ac.uk/interpro/entry/IPR001394", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR018200", "url": "https://www.ebi.ac.uk/interpro/entry/IPR018200", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR028889", "url": "https://www.ebi.ac.uk/interpro/entry/IPR028889", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR038765", "url": "https://www.ebi.ac.uk/interpro/entry/IPR038765", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "ddi:DDB_G0275021", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+ddi:DDB_G0275021", "database": "KEGG Pathway", "categories": [ "Enzyme and Pathway" ] }, { "id": "INTSNTC", "url": "https://omabrowser.org/oma/group/INTSNTC/", "database": "OMA", "categories": [ "Protein Classification" ] }, { "id": "PTHR24006", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR24006", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PTHR24006:SF687", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR24006:SF687", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PF00443", "url": "http://pfam.xfam.org/family/PF00443", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PR_O96612", "url": "http://purl.obolibrary.org/obo/PR_O96612", "database": "PRO", "categories": [ "Protein" ] }, { "id": "R-DDI-1358803", "url": "https://www.reactome.org/PathwayBrowser/#R-DDI-1358803", "database": "Reactome", "categories": [ "Protein Classification" ] }, { "id": "XP_643807", "url": "https://www.ncbi.nlm.nih.gov/protein/XP_643807", "database": "RefSeq", "categories": [ "Protein" ] }, { "id": "O96612", "url": "https://www.uniprot.org/uniprot/O96612", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "O96612-1", "url": "https://www.uniprot.org/uniprot/O96612-1", "database": "UniProtKB", "categories": [ "Protein" ] } ], "sequence": { "sequence": "MKNIEEMPSKSQLVAHRSRGLINTSNTCFMNVILQSLTGCQLFLRVIKNLSDLEDLGKYPTLHSFNQFYTEYFSNPTSNILNNNSNSTTTTSSSSTTATTTSTSNNNKSQTPTSPIQQHHQSQTNGLSNQPSVATQSQQQQQPQPPINPKHFNDLVKSFNSKVSPVPQTTVSPHSMVQVSKKKLKLQLYNNSLPQTICQQDAQEFLVFLLDLIHEEFLTLIKDIDIPKEDDKSTPTSTSIVDDNWEVVGKKGKTAIITNSQQELPKTPISQIFSGVLRSSFNRTGSKESITVEPFYCLHLDIRPEEINSLEDALKFFMKPEIIEGYTCSTKKIEISASKSWSFESLPRILIVHFKRFAFESDTSKKLDKLIRFPTQLSLSTASNHQTQKKYSLFSVVSHHGRGLSQGHYTCDIYQPQQAQWIRYDDSTFTEVKEQDVLNREAYLLLYQLVN", "length": 451, "header": "sp|O96612-1|UBPB_DICDI Isoform 1 of Ubiquitin hydrolase B OS=Dictyostelium discoideum OX=44689 GN=ubpB PE=1 SV=1 CANONICAL=O96612-1" }, "isoforms": [ { "isoform_ac": "O96612-1", "url": "", "sequence": { "sequence": "MKNIEEMPSKSQLVAHRSRGLINTSNTCFMNVILQSLTGCQLFLRVIKNLSDLEDLGKYPTLHSFNQFYTEYFSNPTSNILNNNSNSTTTTSSSSTTATTTSTSNNNKSQTPTSPIQQHHQSQTNGLSNQPSVATQSQQQQQPQPPINPKHFNDLVKSFNSKVSPVPQTTVSPHSMVQVSKKKLKLQLYNNSLPQTICQQDAQEFLVFLLDLIHEEFLTLIKDIDIPKEDDKSTPTSTSIVDDNWEVVGKKGKTAIITNSQQELPKTPISQIFSGVLRSSFNRTGSKESITVEPFYCLHLDIRPEEINSLEDALKFFMKPEIIEGYTCSTKKIEISASKSWSFESLPRILIVHFKRFAFESDTSKKLDKLIRFPTQLSLSTASNHQTQKKYSLFSVVSHHGRGLSQGHYTCDIYQPQQAQWIRYDDSTFTEVKEQDVLNREAYLLLYQLVN", "length": 451, "header": "sp|O96612-1|UBPB_DICDI Isoform 1 of Ubiquitin hydrolase B OS=Dictyostelium discoideum OX=44689 GN=ubpB PE=1 SV=1 CANONICAL=O96612-1" }, "locus": {} } ], "glycosylation": [], "phosphorylation": [], "glycation": [], "keywords": [ "enzyme" ], "publication": [ { "title": "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.", "journal": "Nature", "date": "2002", "authors": "Gl\u00f6ckner Gernot, Eichinger Ludwig, Szafranski Karol, Pachebat Justin A, Bankier Alan T, Dear Paul H, Lehmann R\u00fcdiger, Baumgart Cornelia, Parra Gen\u00eds, Abril Josep F, Guig\u00f3 Roderic, Kumpf Kai, Tunggal Budi, Cox Edward, Quail Michael A, Platzer Matthias, Rosenthal Andr\u00e9, Noegel Angelika A,", "evidence": [ { "database": "UniProtKB", "id": "O96612", "url": "https://www.uniprot.org/uniprot/O96612" } ], "reference": [ { "type": "PubMed", "id": "12097910", "url": "https://glygen.org/publication/PubMed/12097910" } ] }, { "title": "The genome of the social amoeba Dictyostelium discoideum.", "journal": "Nature", "date": "2005", "authors": "Eichinger L, Pachebat J A, Gl\u00f6ckner G, Rajandream M-A, Sucgang R, Berriman M, Song J, Olsen R, Szafranski K, Xu Q, Tunggal B, Kummerfeld S, Madera M, Konfortov B A, Rivero F, Bankier A T, Lehmann R, Hamlin N, Davies R, Gaudet P, Fey P, Pilcher K, Chen G, Saunders D, Sodergren E, Davis P, Kerhornou A, Nie X, Hall N, Anjard C, Hemphill L, Bason N, Farbrother P, Desany B, Just E, Morio T, Rost R, Churcher C, Cooper J, Haydock S, van Driessche N, Cronin A, Goodhead I, Muzny D, Mourier T, Pain A, Lu M, Harper D, Lindsay R, Hauser H, James K, Quiles M, Madan Babu M, Saito T, Buchrieser C, Wardroper A, Felder M, Thangavelu M, Johnson D, Knights A, Loulseged H, Mungall K, Oliver K, Price C, Quail M A, Urushihara H, Hernandez J, Rabbinowitsch E, Steffen D, Sanders M, Ma J, Kohara Y, Sharp S, Simmonds M, Spiegler S, Tivey A, Sugano S, White B, Walker D, Woodward J, Winckler T, Tanaka Y, Shaulsky G, Schleicher M, Weinstock G, Rosenthal A, Cox E C, Chisholm R L, Gibbs R, Loomis W F, Platzer M, Kay R R, Williams J, Dear P H, Noegel A A, Barrell B, Kuspa A", "evidence": [ { "database": "UniProtKB", "id": "O96612", "url": "https://www.uniprot.org/uniprot/O96612" } ], "reference": [ { "type": "PubMed", "id": "15875012", "url": "https://glygen.org/publication/PubMed/15875012" } ] }, { "title": "A novel, putative MEK kinase controls developmental timing and spatial patterning in Dictyostelium and is regulated by ubiquitin-mediated protein degradation.", "journal": "Genes & development", "date": "1998-11-15", "authors": "Chung C Y, Reddy T B, Zhou K, Firtel R A", "evidence": [ { "database": "UniProtKB", "id": "O96612", "url": "https://www.uniprot.org/uniprot/O96612" } ], "reference": [ { "type": "PubMed", "id": "9832508", "url": "https://glygen.org/publication/PubMed/9832508" } ] } ], "pathway": [ { "id": "ddi:DDB_G0275021", "name": "", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+ddi:DDB_G0275021", "resource": "KEGG Pathway" }, { "id": "R-DDI-1358803", "name": "Downregulation of ERBB2:ERBB3 signaling", "url": "https://www.reactome.org/PathwayBrowser/#R-DDI-1358803", "resource": "Reactome", "description": "Downregulation of ERBB2:ERBB3 signaling" } ], "orthologs": [], "go_annotation": { "categories": [ { "name": "Biological Process", "total": 2, "go_terms": [ { "name": "proteolysis", "id": "GO:0006508", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006508", "evidence": [ { "database": "UniProtKB", "id": "O96612", "url": "https://www.uniprot.org/uniprot/O96612" } ], "pmid": "" }, { "name": "protein deubiquitination", "id": "GO:0016579", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016579", "evidence": [ { "database": "UniProtKB", "id": "O96612", "url": "https://www.uniprot.org/uniprot/O96612" } ], "pmid": "" } ] }, { "name": "Molecular Function", "total": 1, "go_terms": [ { "name": "cysteine-type deubiquitinase activity", "id": "GO:0004843", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0004843", "evidence": [ { "database": "UniProtKB", "id": "O96612", "url": "https://www.uniprot.org/uniprot/O96612" } ], "pmid": "" } ] } ] }, "pro_annotation": [], "site_annotation": [ { "start_pos": 107, "end_pos": 109, "annotation": "n_glycosylation_sequon" }, { "start_pos": 190, "end_pos": 192, "annotation": "n_glycosylation_sequon" }, { "start_pos": 23, "end_pos": 25, "annotation": "n_glycosylation_sequon" }, { "start_pos": 282, "end_pos": 284, "annotation": "n_glycosylation_sequon" }, { "start_pos": 49, "end_pos": 51, "annotation": "n_glycosylation_sequon" }, { "start_pos": 83, "end_pos": 85, "annotation": "n_glycosylation_sequon" }, { "start_pos": 86, "end_pos": 88, "annotation": "n_glycosylation_sequon" } ], "ptm_annotation": [], "interactions": [], "reactions": [ { "id": "R-DDI-1358791", "name": "Phosphorylation of USP8 by P-AKT", "equation": "", "summary": "This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp", "cellular_location": "cytosol", "input_participant_list": [ "R-DDI-917720" ], "output_participant_list": [ "R-DDI-1358714" ], "enzyme_list": [ "Q54IF2-1", "O96612-1", "P54644-1" ], "evidence": [ { "id": "R-DDI-1358791", "database": "Reactome", "url": "https://www.reactome.org/PathwayBrowser/#R-DDI-1358791" } ] }, { "id": "R-DDI-1358795", "name": "Deubiquitination of RNF41 by P-USP8", "equation": "", "summary": "This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp", "cellular_location": "cytosol", "input_participant_list": [], "output_participant_list": [], "enzyme_list": [ "O96612-1" ], "evidence": [ { "id": "R-DDI-1358795", "database": "Reactome", "url": "https://www.reactome.org/PathwayBrowser/#R-DDI-1358795" } ] }, { "id": "R-DDI-1358797", "name": "Ubiquitinated RNF41 binds P-USP8", "equation": "", "summary": "This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp", "cellular_location": "cytosol", "input_participant_list": [], "output_participant_list": [], "enzyme_list": [ "O96612-1" ], "evidence": [ { "id": "R-DDI-1358797", "database": "Reactome", "url": "https://www.reactome.org/PathwayBrowser/#R-DDI-1358797" } ] } ], "reaction_participants": [ { "participant_id": "R-DDI-1358714", "participant_name": "phospho-ubpB [cytosol]", "crossref": [ { "id": "O96612", "database": "UniProt", "url": "" }, { "id": "XP_643807.1", "database": "RefSeq", "url": "" }, { "id": "O96612", "database": "PRO", "url": "" } ] }, { "participant_id": "R-DDI-917720", "participant_name": "ubpB [cytosol]", "crossref": [ { "id": "O96612", "database": "PRO", "url": "" }, { "id": "O96612", "database": "UniProt", "url": "" }, { "id": "XP_643807.1", "database": "RefSeq", "url": "" } ] } ], "reaction_enzymes": [ { "uniprot_ac": "O96612", "gene_name": "ubpB", "activity": [ { "ec_number": "3.4.19.12", "activity": "Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).", "evidence": [ { "id": "O96612", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/O96612" } ] }, { "ec_number": "3.4.19.12", "activity": "", "evidence": [ { "id": "O96612", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/O96612" } ] } ] }, { "uniprot_ac": "P54644", "gene_name": "pkbA", "activity": [ { "ec_number": "2.7.11.1", "activity": "ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein].", "evidence": [ { "id": "P54644", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P54644" } ] }, { "ec_number": "2.7.11.1", "activity": "", "evidence": [ { "id": "P54644", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P54644" } ] } ] }, { "uniprot_ac": "Q54IF2", "gene_name": "", "activity": [ { "ec_number": "2.7.11.1", "activity": "ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein].", "evidence": [ { "id": "Q54IF2", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q54IF2" } ] }, { "ec_number": "2.7.11.1", "activity": "", "evidence": [ { "id": "Q54IF2", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q54IF2" } ] } ] } ], "biomarkers": [], "synthesized_glycans": [], "cluster_types": [], "sequence_features": { "n_linked_sites": [], "o_linked_sites": [], "snv_sites": [], "glycation_sites": [], "phosphorylation_sites": [], "sequon_annotation_sites": [ { "start_pos": 107, "end_pos": 109 }, { "start_pos": 190, "end_pos": 192 }, { "start_pos": 23, "end_pos": 25 }, { "start_pos": 282, "end_pos": 284 }, { "start_pos": 49, "end_pos": 51 }, { "start_pos": 83, "end_pos": 85 }, { "start_pos": 86, "end_pos": 88 } ] }, "section_stats": [ { "table_id": "glycosylation", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 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