",
"cellular_location": "nucleoplasm",
"input_participant_list": [],
"output_participant_list": [],
"enzyme_list": [
"P18074-1",
"P24928-1",
"P30876-1",
"P50613-1",
"P19447-1",
"O43148-1"
],
"evidence": [
{
"id": "R-HSA-77078",
"database": "Reactome",
"url": "https://www.reactome.org/PathwayBrowser/#R-HSA-77078"
}
]
},
{
"id": "R-HSA-77081",
"name": "Formation of the CE:GMP intermediate complex",
"equation": "",
"summary": "A highly conserved lysine within the guanylyltransferase (GT) site of the mRNA capping enzyme attacks the alpha-phosphate of GTP. An enzyme-GMP covalent intermediate is formed.
",
"cellular_location": "nucleoplasm",
"input_participant_list": [],
"output_participant_list": [],
"enzyme_list": [
"P18074-1",
"P24928-1",
"P30876-1",
"P50613-1",
"P19447-1",
"O43148-1"
],
"evidence": [
{
"id": "R-HSA-77081",
"database": "Reactome",
"url": "https://www.reactome.org/PathwayBrowser/#R-HSA-77081"
}
]
},
{
"id": "R-HSA-77083",
"name": "Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA",
"equation": "",
"summary": "The diphosphate 5'-end of the mRNA is joined to the GMP, releasing it from the enzyme. At this time, it is unclear how the RNA diphosphate end is transferred from the active site of the triphosphatase to the guanylyltransferase site. The covalent enzyme-GMP complex can form in the absence of RNA.
Guanylyltransferase (GT) catalyzed second reaction can be represented as:ppN(pN)n + GTP -> GpppN(pN)n + PPi
; (Yamada-Okabe et al. 1998).",
"cellular_location": "nucleoplasm",
"input_participant_list": [],
"output_participant_list": [],
"enzyme_list": [
"P18074-1",
"P24928-1",
"P30876-1",
"P50613-1",
"P19447-1",
"O43148-1"
],
"evidence": [
{
"id": "R-HSA-77083",
"database": "Reactome",
"url": "https://www.reactome.org/PathwayBrowser/#R-HSA-77083"
}
]
},
{
"id": "R-HSA-77085",
"name": "Dissociation of transcript with 5'-GMP from GT",
"equation": "",
"summary": "GMP capped mRNA transcript dissociates from GT for further modification (Yamada-Okabe et al. 1998).",
"cellular_location": "nucleoplasm",
"input_participant_list": [],
"output_participant_list": [],
"enzyme_list": [
"P18074-1",
"P24928-1",
"P30876-1",
"P50613-1",
"P19447-1",
"O43148-1"
],
"evidence": [
{
"id": "R-HSA-77085",
"database": "Reactome",
"url": "https://www.reactome.org/PathwayBrowser/#R-HSA-77085"
}
]
},
{
"id": "R-HSA-77090",
"name": "Methylation of GMP-cap by RNA Methyltransferase",
"equation": "",
"summary": "In the final step of the capping reaction, the methyltransferase takes a methyl group from S-adenosyl-methionine to the N7 position of the cap guanine. N7G-methyltransferase (MT) mediated reaction can be represented as:
GpppN(pN)n + S-adenosylmethionine (Adomet) ->m7GpppN(pN)n + S-adenosylhomocysteine (Adohcy).
", "cellular_location": "nucleoplasm", "input_participant_list": [], "output_participant_list": [], "enzyme_list": [ "P18074-1", "P24928-1", "P30876-1", "P50613-1", "P19447-1", "O43148-1" ], "evidence": [ { "id": "R-HSA-77090", "database": "Reactome", "url": "https://www.reactome.org/PathwayBrowser/#R-HSA-77090" } ] } ], "reaction_participants": [], "reaction_enzymes": [ { "uniprot_ac": "O43148", "gene_name": "RNMT", "activity": [ { "ec_number": "2.1.1.56", "activity": "a 5'-end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine.", "evidence": [ { "id": "O43148", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/O43148" } ] }, { "ec_number": "2.1.1.56", "activity": "", "evidence": [ { "id": "O43148", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/O43148" } ] } ] }, { "uniprot_ac": "P18074", "gene_name": "ERCC2", "activity": [ { "ec_number": "3.6.4.12", "activity": "ATP + H2O = ADP + H(+) + phosphate.", "evidence": [ { "id": "P18074", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P18074" } ] }, { "ec_number": "3.6.4.12", "activity": "", "evidence": [ { "id": "P18074", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P18074" } ] } ] }, { "uniprot_ac": "P19447", "gene_name": "ERCC3", "activity": [ { "ec_number": "3.6.4.12", "activity": "ATP + H2O = ADP + H(+) + phosphate.", "evidence": [ { "id": "P19447", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P19447" } ] }, { "ec_number": "3.6.4.12", "activity": "", "evidence": [ { "id": "P19447", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P19447" } ] } ] }, { "uniprot_ac": "P24928", "gene_name": "POLR2A", "activity": [ { "ec_number": "2.7.7.6", "activity": "a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1).", "evidence": [ { "id": "P24928", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P24928" } ] }, { "ec_number": "2.7.7.6", "activity": "", "evidence": [ { "id": "P24928", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P24928" } ] }, { "ec_number": "2.7.7.48", "activity": "a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1).", "evidence": [ { "id": "P24928", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P24928" } ] } ] }, { "uniprot_ac": "P30876", "gene_name": "POLR2B", "activity": [ { "ec_number": "2.7.7.6", "activity": "a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1).", "evidence": [ { "id": "P30876", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P30876" } ] }, { "ec_number": "2.7.7.6", "activity": "", "evidence": [ { "id": "P30876", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P30876" } ] } ] }, { "uniprot_ac": "P32783", "gene_name": "ABD1", "activity": [ { "ec_number": "2.1.1.56", "activity": "a 5'-end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine.", "evidence": [ { "id": "P32783", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P32783" } ] }, { "ec_number": "2.1.1.56", "activity": "", "evidence": [ { "id": "P32783", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P32783" } ] } ] }, { "uniprot_ac": "P50613", "gene_name": "CDK7", "activity": [ { "ec_number": "2.7.11.23", "activity": "[DNA-directed RNA polymerase] + ATP = ADP + H(+) + phospho-[DNA-directed RNA polymerase].", "evidence": [ { "id": "P50613", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P50613" } ] }, { "ec_number": "2.7.11.23", "activity": "", "evidence": [ { "id": "P50613", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P50613" } ] }, { "ec_number": "2.7.11.22", "activity": "ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein].", "evidence": [ { "id": "P50613", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P50613" } ] }, { "ec_number": "2.7.11.22", "activity": "", "evidence": [ { "id": "P50613", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P50613" } ] } ] }, { "uniprot_ac": "Q54IL6", "gene_name": "", "activity": [ { "ec_number": "2.1.1.56", "activity": "a 5'-end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine.", "evidence": [ { "id": "Q54IL6", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q54IL6" } ] }, { "ec_number": "2.1.1.56", "activity": "", "evidence": [ { "id": "Q54IL6", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q54IL6" } ] } ] }, { "uniprot_ac": "Q54Y13", "gene_name": "", "activity": [ { "ec_number": "2.1.1.56", "activity": "a 5'-end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine.", "evidence": [ { "id": "Q54Y13", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q54Y13" } ] }, { "ec_number": "2.1.1.56", "activity": "", "evidence": [ { "id": "Q54Y13", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q54Y13" } ] } ] }, { "uniprot_ac": "Q5U2U7", "gene_name": "Rnmt", "activity": [ { "ec_number": "2.1.1.56", "activity": "a 5'-end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine.", "evidence": [ { "id": "Q5U2U7", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q5U2U7" } ] }, { "ec_number": "2.1.1.56", "activity": "", "evidence": [ { "id": "Q5U2U7", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q5U2U7" } ] } ] }, { "uniprot_ac": "Q9D0L8", "gene_name": "Rnmt", "activity": [ { "ec_number": "2.1.1.56", "activity": "a 5'-end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine.", "evidence": [ { "id": "Q9D0L8", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q9D0L8" } ] }, { "ec_number": "2.1.1.56", "activity": "", "evidence": [ { "id": "Q9D0L8", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q9D0L8" } ] } ] }, { "uniprot_ac": "Q9VJQ4", "gene_name": "Rnmt", "activity": [ { "ec_number": "2.1.1.56", "activity": "a 5'-end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine.", "evidence": [ { "id": "Q9VJQ4", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q9VJQ4" } ] }, { "ec_number": "2.1.1.56", "activity": "", "evidence": [ { "id": "Q9VJQ4", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q9VJQ4" } ] } ] } ], "biomarkers": [], "synthesized_glycans": [], "cluster_types": [ { "name": "isoformset.uniprotkb", "label": "Isoforms" }, { "name": "homologset.mgi", "label": "MGI Homologs" }, { "name": "homologset.oma", "label": "OMA Homologs" } ], "sequence_features": { "n_linked_sites": [], "o_linked_sites": [], "snv_sites": [ 272 ], "glycation_sites": [], "phosphorylation_sites": [ 10, 24, 28, 29, 77, 79, 118, 232 ], "sequon_annotation_sites": [ { "start_pos": 35, "end_pos": 37 }, { "start_pos": 424, "end_pos": 426 } ] }, "section_stats": [ { "table_id": "glycosylation", "table_stats": [ { "field": "total", "count": 1 }, { "field": "total_sites", "count": 1 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycans", "count": 1 }, { "field": "o_linked_glycan_sites", "count": 1 } ], "sort_fields": [ 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"count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_automatic_literature_mining", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "phosphorylation", "table_stats": [ { "field": "total", "count": 9 }, { "field": "total_sites", "count": 8 } ], "sort_fields": [ "start_pos", "end_pos", "kinase_uniprot_canonical_ac", "kinase_gene_name", "residue", "comment", "site_lbl" ] }, { "table_id": "snv", "table_stats": [ { "field": "total", "count": 1 }, { "field": "total_sites", "count": 1 } ], "sort_fields": [ "start_pos", "end_pos", "subjects_tested", "subjects_positive", "frequency", "sequence_org", "sequence_mut", "comment", "chr_id", "chr_pos", "ref_nt", "alt_nt", "minor_allelic_frequency", "site_lbl" ] }, { "table_id": "snv_disease", "table_stats": [ { "field": "total", "count": 1 }, { "field": "total_sites", "count": 1 } ], "sort_fields": [ "start_pos", "end_pos", "subjects_tested", "subjects_positive", "frequency", "sequence_org", "sequence_mut", "comment", "chr_id", "chr_pos", "ref_nt", "alt_nt", "minor_allelic_frequency", "site_lbl" ] }, { "table_id": "snv_non_disease", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "publication", "table_stats": [ { "field": "total", "count": 28 } ], "sort_fields": [ "title", "journal", "date", "authors" ] } ] }