{
    "uniprot_canonical_ac": "B0G132-1",
    "uniprot_ac": "B0G132",
    "uniprot_id": "DPH2_DICDI",
    "mass": {
        "chemical_mass": 67193.0,
        "monoisotopic_mass": 67193.0
    },
    "refseq": {
        "ac": "XP_001732995",
        "name": "diphthamide biosynthesis protein 2",
        "summary": "",
        "url": "https://www.ncbi.nlm.nih.gov/protein/XP_001732995"
    },
    "gene_names": [
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            "name": "dph2",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "https://www.uniprot.org/uniprot/B0G132",
            "id": "B0G132"
        },
        {
            "name": "DDB_G0295687",
            "resource": "UniProtKB",
            "type": "synonym",
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            "id": "B0G132"
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        {
            "name": "dph2",
            "resource": "RefSeq",
            "type": "synonym",
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            "id": "XP_001732995"
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    ],
    "protein_names": [
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            "name": "2-(3-amino-3-carboxypropyl)histidine synthase subunit 2",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "",
            "id": "B0G132"
        },
        {
            "name": "Diphthamide biosynthesis protein 2",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "",
            "id": "B0G132"
        },
        {
            "name": "Diphtheria toxin resistance protein 2",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "",
            "id": "B0G132"
        },
        {
            "name": "S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 2",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "",
            "id": "B0G132"
        },
        {
            "name": "diphthamide biosynthesis protein 2",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/XP_001732995",
            "id": "XP_001732995"
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    ],
    "function": [
        {
            "annotation": "Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2 (By similarity). Dph1 and dph2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising dph3 and a NADH-dependent reductase (By similarity). Facilitates the reduction of the catalytic iron-sulfur cluster found in the dph1 subunit (By similarity).",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "B0G132",
                    "url": "https://www.uniprot.org/uniprot/B0G132#function"
                },
                {
                    "database": "UniProtKB",
                    "id": "P32461",
                    "url": "https://www.uniprot.org/uniprot/P32461#function"
                }
            ]
        }
    ],
    "disease": [],
    "snv": [],
    "mutagenesis": [],
    "expression_tissue": [],
    "expression_disease": [],
    "species": [
        {
            "name": "Dictyostelium discoideum",
            "taxid": 44689,
            "evidence": [
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                    "database": "UniProtKB",
                    "id": "B0G132",
                    "url": "https://www.uniprot.org/uniprot/B0G132"
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            "common_name": "Cellular slime molds"
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            "name": "dph2",
            "url": "https://www.uniprot.org/uniprot/B0G132",
            "locus": {}
        }
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    "crossref": [
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            "id": "DDB_G0295687",
            "url": "http://dictybase.org/gene/DDB_G0295687",
            "database": "dictyBase",
            "categories": [
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        },
        {
            "id": "8622261",
            "url": "https://www.ncbi.nlm.nih.gov/gene/8622261",
            "database": "GeneID",
            "categories": [
                "Genome Annotation"
            ]
        },
        {
            "id": "IPR010014",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR010014",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR016435",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR016435",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR042263",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR042263",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR042265",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR042265",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "ddi:DDB_G0295687",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+ddi:DDB_G0295687",
            "database": "KEGG Pathway",
            "categories": [
                "Enzyme and Pathway"
            ]
        },
        {
            "id": "IEGWVVV",
            "url": "https://omabrowser.org/oma/group/IEGWVVV/",
            "database": "OMA",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR10762",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR10762",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR10762:SF2",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR10762:SF2",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PF01866",
            "url": "http://pfam.xfam.org/family/PF01866",
            "database": "Pfam",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PR_B0G132",
            "url": "http://purl.obolibrary.org/obo/PR_B0G132",
            "database": "PRO",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "R-DDI-5358493",
            "url": "https://www.reactome.org/PathwayBrowser/#R-DDI-5358493",
            "database": "Reactome",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "XP_001732995",
            "url": "https://www.ncbi.nlm.nih.gov/protein/XP_001732995",
            "database": "RefSeq",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "B0G132",
            "url": "https://www.uniprot.org/uniprot/B0G132",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "B0G132-1",
            "url": "https://www.uniprot.org/uniprot/B0G132-1",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        }
    ],
    "sequence": {
        "sequence": "MSAAPPPSIYTESFSTSSNNTTESESINVEETRENILSGFSFEEYFQVSESISVIIKNNFKKIALQLPDYLLWASSELSNKIQNSVPLEYGIKVFILGDTSYGSCCVDEVTSSHLKSDFIIHYGHSCLSPSSKIPVQYVFGKKKNFNVDKFSIELNKLVESKKEENFVIFYDLLYHHNRDSIENLYKNNDNIIVTNINNFNNIYLNNSFKNNKLNETQNCNNNYNNDCCGSDNNNNNNDSGCCGGNNNNNNNNSGCCQSQSQQQQQQNDCCQQKLNNEINNENDNNNEELISGRKIEFKNEKDIKKFNFIWIGEESLTLTNLLMNFNQQMVYRYYIDDNKLLQETLPRNKSLMRRYHISEKCKDASIIGIVVSTLSVQKYSETIDGLKKLIIASGKKPYVFVVGRLNVPKLANFSEIDIYVIVACHENTMVDSKDFYKPIATPFELNLSLRNEKWTGEYITDFGRVFPKLIKDLSFDEDDENKCEIPEDQVEDDQGNIHHFSLVTGRIVIQNKNQSTLQQQQQQQQTQQSGDLISVNNQFKQVSLVGEHLSNKTYKGLDMRIGETPVTTAIEGMSGIPKNYNTDKNNL",
        "length": 588,
        "header": "sp|B0G132-1|DPH2_DICDI Isoform 1 of 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2  OS=Dictyostelium discoideum OX=44689 GN=dph2 PE=3 SV=1 CANONICAL=B0G132-1"
    },
    "isoforms": [
        {
            "isoform_ac": "B0G132-1",
            "url": "",
            "sequence": {
                "sequence": "MSAAPPPSIYTESFSTSSNNTTESESINVEETRENILSGFSFEEYFQVSESISVIIKNNFKKIALQLPDYLLWASSELSNKIQNSVPLEYGIKVFILGDTSYGSCCVDEVTSSHLKSDFIIHYGHSCLSPSSKIPVQYVFGKKKNFNVDKFSIELNKLVESKKEENFVIFYDLLYHHNRDSIENLYKNNDNIIVTNINNFNNIYLNNSFKNNKLNETQNCNNNYNNDCCGSDNNNNNNDSGCCGGNNNNNNNNSGCCQSQSQQQQQQNDCCQQKLNNEINNENDNNNEELISGRKIEFKNEKDIKKFNFIWIGEESLTLTNLLMNFNQQMVYRYYIDDNKLLQETLPRNKSLMRRYHISEKCKDASIIGIVVSTLSVQKYSETIDGLKKLIIASGKKPYVFVVGRLNVPKLANFSEIDIYVIVACHENTMVDSKDFYKPIATPFELNLSLRNEKWTGEYITDFGRVFPKLIKDLSFDEDDENKCEIPEDQVEDDQGNIHHFSLVTGRIVIQNKNQSTLQQQQQQQQTQQSGDLISVNNQFKQVSLVGEHLSNKTYKGLDMRIGETPVTTAIEGMSGIPKNYNTDKNNL",
                "length": 588,
                "header": "sp|B0G132-1|DPH2_DICDI Isoform 1 of 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2  OS=Dictyostelium discoideum OX=44689 GN=dph2 PE=3 SV=1 CANONICAL=B0G132-1"
            },
            "locus": {}
        }
    ],
    "glycosylation": [],
    "phosphorylation": [],
    "glycation": [],
    "keywords": [],
    "publication": [
        {
            "title": "The genome of the social amoeba Dictyostelium discoideum.",
            "journal": "Nature",
            "date": "2005",
            "authors": "Eichinger L, Pachebat J A, Gl\u00f6ckner G, Rajandream M-A, Sucgang R, Berriman M, Song J, Olsen R, Szafranski K, Xu Q, Tunggal B, Kummerfeld S, Madera M, Konfortov B A, Rivero F, Bankier A T, Lehmann R, Hamlin N, Davies R, Gaudet P, Fey P, Pilcher K, Chen G, Saunders D, Sodergren E, Davis P, Kerhornou A, Nie X, Hall N, Anjard C, Hemphill L, Bason N, Farbrother P, Desany B, Just E, Morio T, Rost R, Churcher C, Cooper J, Haydock S, van Driessche N, Cronin A, Goodhead I, Muzny D, Mourier T, Pain A, Lu M, Harper D, Lindsay R, Hauser H, James K, Quiles M, Madan Babu M, Saito T, Buchrieser C, Wardroper A, Felder M, Thangavelu M, Johnson D, Knights A, Loulseged H, Mungall K, Oliver K, Price C, Quail M A, Urushihara H, Hernandez J, Rabbinowitsch E, Steffen D, Sanders M, Ma J, Kohara Y, Sharp S, Simmonds M, Spiegler S, Tivey A, Sugano S, White B, Walker D, Woodward J, Winckler T, Tanaka Y, Shaulsky G, Schleicher M, Weinstock G, Rosenthal A, Cox E C, Chisholm R L, Gibbs R, Loomis W F, Platzer M, Kay R R, Williams J, Dear P H, Noegel A A, Barrell B, Kuspa A",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "B0G132",
                    "url": "https://www.uniprot.org/uniprot/B0G132"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "15875012",
                    "url": "https://glygen.org/publication/PubMed/15875012"
                }
            ]
        }
    ],
    "pathway": [
        {
            "id": "ddi:DDB_G0295687",
            "name": "",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+ddi:DDB_G0295687",
            "resource": "KEGG Pathway"
        },
        {
            "id": "R-DDI-5358493",
            "name": "Synthesis of diphthamide-EEF2",
            "url": "https://www.reactome.org/PathwayBrowser/#R-DDI-5358493",
            "resource": "Reactome",
            "description": "Synthesis of diphthamide-EEF2"
        }
    ],
    "orthologs": [],
    "go_annotation": {
        "categories": [
            {
                "name": "Molecular Function",
                "total": 3,
                "go_terms": [
                    {
                        "name": "metal ion binding",
                        "id": "GO:0046872",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046872",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "B0G132",
                                "url": "https://www.uniprot.org/uniprot/B0G132"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "4 iron, 4 sulfur cluster binding",
                        "id": "GO:0051539",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0051539",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "B0G132",
                                "url": "https://www.uniprot.org/uniprot/B0G132"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "2-(3-amino-3-carboxypropyl)histidine synthase activity",
                        "id": "GO:0090560",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0090560",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "B0G132",
                                "url": "https://www.uniprot.org/uniprot/B0G132"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            },
            {
                "name": "Cellular Component",
                "total": 1,
                "go_terms": [
                    {
                        "name": "protein-containing complex",
                        "id": "GO:0032991",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0032991",
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                            {
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                                "id": "B0G132",
                                "url": "https://www.uniprot.org/uniprot/B0G132"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            },
            {
                "name": "Biological Process",
                "total": 1,
                "go_terms": [
                    {
                        "name": "protein histidyl modification to diphthamide",
                        "id": "GO:0017183",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0017183",
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    "reaction_enzymes": [],
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    "synthesized_glycans": [],
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