{ "uniprot_canonical_ac": "P0DTU4-1", "uniprot_ac": "P0DTU4", "uniprot_id": "TRBR2_HUMAN", "mass": { "chemical_mass": 35490.0, "monoisotopic_mass": 35490.0 }, "refseq": {}, "gene_names": [ { "name": "TRB", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/P0DTU4", "id": "P0DTU4" } ], "protein_names": [ { "name": "T cell receptor beta chain MC.7.G5", "resource": "UniProtKB", "type": "recommended", "url": "", "id": "P0DTU4" }, { "name": "MC.7.G5 TRB", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "P0DTU4" }, { "name": "TR beta chain TRBV25-1*01J2S3*01C2*01", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "P0DTU4" } ], "function": [ { "annotation": "The beta chain of TRAV38-2DV8*01J31*01C*01/TRBV25-1*01J2S3*01C2*01 alpha-beta T cell receptor (TR) clonotype that displays pan-cancer cell recognition via the invariant MR1 molecule. On CD8-positive T cell clone MC.7.G5, likely recognizes tumor-specific or -associated metabolite(s) essential for cancer cell survival, triggering killing of many cancer cell types including lung, melanoma, leukemia, colon, breast, prostate, bone and ovarian cancer cells. Mediates cancer cell cytotoxicity in an HLA-independent manner. Has no reactivity to healthy cells even stressed or infected by bacteria (PubMed:31959982). Antigen recognition initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell differentiation into effector/memory T cells (By similarity).", "evidence": [ { "database": "UniProtKB", "id": "P0DTU4", "url": "https://www.uniprot.org/uniprot/P0DTU4#function" }, { "database": "UniProtKB", "id": "P01848", "url": "https://www.uniprot.org/uniprot/P01848#function" }, { "database": "PubMed", "id": "31959982", "url": "https://glygen.org/publication/PubMed/31959982" } ] } ], "disease": [], "snv": [], "mutagenesis": [], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Homo sapiens", "taxid": 9606, "evidence": [ { "database": "UniProtKB", "id": "P0DTU4", "url": "https://www.uniprot.org/uniprot/P0DTU4" } ], "common_name": "Human" } ], "gene": [ { "name": "TRB", "url": "https://www.uniprot.org/uniprot/P0DTU4", "locus": {} } ], "crossref": [ { "id": "cd05769", "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd05769", "database": "CDD", "categories": [ "Protein Classification" ] }, { "id": "cd05899", "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd05899", "database": "CDD", "categories": [ "Protein Classification" ] }, { "id": "TRB", "url": "http://www.genecards.org/cgi-bin/carddisp.pl?gene=TRB", "database": "GeneCards", "categories": [ "Gene Annotation", "Organism-specific" ] }, { "id": "12155", "url": "https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=12155", "database": "HGNC", "categories": [ "Organism-specific" ] }, { "id": "IPR003597", "url": "https://www.ebi.ac.uk/interpro/entry/IPR003597", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR003599", "url": "https://www.ebi.ac.uk/interpro/entry/IPR003599", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR007110", "url": "https://www.ebi.ac.uk/interpro/entry/IPR007110", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR013106", "url": "https://www.ebi.ac.uk/interpro/entry/IPR013106", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR013783", "url": "https://www.ebi.ac.uk/interpro/entry/IPR013783", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR036179", "url": "https://www.ebi.ac.uk/interpro/entry/IPR036179", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "NX_P0DTU4", "url": "https://www.nextprot.org/entry/NX_P0DTU4", "database": "neXtProt", "categories": [ "Protein", "Organism-specific" ] }, { "id": "PTHR23268", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR23268", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PTHR23268:SF30", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR23268:SF30", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PF07654", "url": "http://pfam.xfam.org/family/PF07654", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PF07686", "url": "http://pfam.xfam.org/family/PF07686", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "P0DTU4", "url": "https://www.uniprot.org/uniprot/P0DTU4", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "P0DTU4-1", "url": "https://www.uniprot.org/uniprot/P0DTU4-1", "database": "UniProtKB", "categories": [ "Protein" ] } ], "sequence": { "sequence": "MTIRLLCYMGFYFLGAGLMEADIYQTPRYLVIGTGKKITLECSQTMGHDKMYWYQQDPGMELHLIHYSYGVNSTEKGDLSSESTVSRIRTEHFPLTLESARPSHTSQYLCASSEARGLAEFTDTQYFGPGTRLTVLEDLKNVFPPEVAVFEPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVSTDPQPLKEQPALNDSRYCLSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRADCGFTSESYQQGVLSATILYEILLGKATLYAVLVSALVLMAMVKRKDSRG", "length": 315, "header": "sp|P0DTU4-1|TRBR2_HUMAN Isoform 1 of T cell receptor beta chain MC.7.G5 OS=Homo sapiens OX=9606 GN=TRB PE=1 SV=1 CANONICAL=P0DTU4-1" }, "isoforms": [ { "isoform_ac": "P0DTU4-1", "url": "", "sequence": { "sequence": "MTIRLLCYMGFYFLGAGLMEADIYQTPRYLVIGTGKKITLECSQTMGHDKMYWYQQDPGMELHLIHYSYGVNSTEKGDLSSESTVSRIRTEHFPLTLESARPSHTSQYLCASSEARGLAEFTDTQYFGPGTRLTVLEDLKNVFPPEVAVFEPSEAEISHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVSTDPQPLKEQPALNDSRYCLSSRLRVSATFWQNPRNHFRCQVQFYGLSENDEWTQDRAKPVTQIVSAEAWGRADCGFTSESYQQGVLSATILYEILLGKATLYAVLVSALVLMAMVKRKDSRG", "length": 315, "header": "sp|P0DTU4-1|TRBR2_HUMAN Isoform 1 of T cell receptor beta chain MC.7.G5 OS=Homo sapiens OX=9606 GN=TRB PE=1 SV=1 CANONICAL=P0DTU4-1" }, "locus": {} } ], "glycosylation": [ { "glytoucan_ac": "", "type": "N-linked", "subtype": "", "site_category": "reported", "site_seq": "N", "relation": "attached", "comment": "N-linked (GlcNAc...) asparagine", "start_pos": 206, "start_aa": "Asn", "end_pos": 206, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn206", "evidence": [ { "id": "P0DTU4", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P0DTU4#ptm_processing" }, { "id": "PRU00498", "database": "PROSITE-PRORULE", "url": "https://prosite.expasy.org/rule/PRU00498" } ] }, { "glytoucan_ac": "", "type": "N-linked", "subtype": "", "site_category": "reported", "site_seq": "N", "relation": "attached", "comment": "N-linked (GlcNAc...) asparagine", "start_pos": 72, "start_aa": "Asn", "end_pos": 72, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn72", "evidence": [ { "id": "P0DTU4", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P0DTU4#ptm_processing" }, { "id": "PRU00498", "database": "PROSITE-PRORULE", "url": "https://prosite.expasy.org/rule/PRU00498" } ] } ], "phosphorylation": [], "glycation": [], "keywords": [ "glycoprotein", "glycoprotein_reported" ], "publication": [ { "title": "Protein displays of the human T cell receptor alpha, beta, gamma and delta variable and joining regions.", "journal": "Exp. Clin. Immunogenet.", "date": "2000", "authors": "Folch G, Scaviner D, Contet V, Lefranc M P", "evidence": [ { "database": "UniProtKB", "id": "P0DTU4", "url": "https://www.uniprot.org/uniprot/P0DTU4" } ], "reference": [ { "type": "PubMed", "id": "11096259", "url": "https://glygen.org/publication/PubMed/11096259" } ] }, { "title": "A clonotype nomenclature for T cell receptors.", "journal": "Immunogenetics", "date": "2009", "authors": "Yassai Maryam B, Naumov Yuri N, Naumova Elena N, Gorski Jack", "evidence": [ { "database": "UniProtKB", "id": "P0DTU4", "url": "https://www.uniprot.org/uniprot/P0DTU4" } ], "reference": [ { "type": "PubMed", "id": "19568742", "url": "https://glygen.org/publication/PubMed/19568742" } ] }, { "title": "Genome-wide CRISPR-Cas9 screening reveals ubiquitous T cell cancer targeting via the monomorphic MHC class I-related protein MR1.", "journal": "Nature immunology", "date": "2020", "authors": "Crowther MD, Dolton G, Legut M, Caillaud ME, Lloyd A, Attaf M, Galloway SAE, Rius C, Farrell CP, Szomolay B, Ager A, Parker AL, Fuller A, Donia M, McCluskey J, Rossjohn J, Svane IM, Phillips JD, Sewell AK", "evidence": [ { "database": "UniProtKB", "id": "P0DTU4", "url": "https://www.uniprot.org/uniprot/P0DTU4" } ], "reference": [ { "type": "PubMed", "id": "31959982", "url": "https://glygen.org/publication/PubMed/31959982" } ] }, { "title": "Sequence and evolution of the human T-cell antigen receptor beta-chain genes.", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "date": "1985", "authors": "Tunnacliffe A, Kefford R, Milstein C, Forster A, Rabbitts TH", "evidence": [ { "database": "UniProtKB", "id": "P0DTU4", "url": "https://www.uniprot.org/uniprot/P0DTU4" } ], "reference": [ { "type": "PubMed", "id": "3860845", "url": "https://glygen.org/publication/PubMed/3860845" } ] } ], "pathway": [], "orthologs": [], "go_annotation": { "categories": [ { "name": "Cellular Component", "total": 2, "go_terms": [ { "name": "plasma membrane", "id": "GO:0005886", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005886", "evidence": [ { "database": "UniProtKB", "id": "P0DTU4", "url": "https://www.uniprot.org/uniprot/P0DTU4" } ], "pmid": "" }, { "name": "alpha-beta T cell receptor complex", "id": "GO:0042105", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042105", "evidence": [ { "database": "UniProtKB", "id": "P0DTU4", "url": "https://www.uniprot.org/uniprot/P0DTU4" } ], "pmid": "" } ] }, { "name": "Biological Process", "total": 3, "go_terms": [ { "name": "cell surface receptor signaling pathway", "id": "GO:0007166", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0007166", "evidence": [ { "database": "UniProtKB", "id": "P0DTU4", "url": "https://www.uniprot.org/uniprot/P0DTU4" } ], "pmid": "" }, { "name": "detection of tumor cell", "id": "GO:0002355", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0002355", "evidence": [ { "database": "UniProtKB", "id": "P0DTU4", "url": "https://www.uniprot.org/uniprot/P0DTU4" } ], "pmid": "" }, { "name": "T cell mediated cytotoxicity directed against tumor cell target", "id": "GO:0002419", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0002419", "evidence": [ { "database": "UniProtKB", "id": "P0DTU4", "url": "https://www.uniprot.org/uniprot/P0DTU4" } ], "pmid": "" } ] }, { "name": "Molecular Function", "total": 1, "go_terms": [ { "name": "signaling receptor activity", "id": "GO:0038023", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0038023", "evidence": [ { "database": "UniProtKB", "id": "P0DTU4", "url": "https://www.uniprot.org/uniprot/P0DTU4" } ], "pmid": "" } ] } ] }, "pro_annotation": [], "site_annotation": [ { "start_pos": 206, "end_pos": 208, "annotation": "n_glycosylation_sequon" }, { "start_pos": 72, "end_pos": 74, "annotation": "n_glycosylation_sequon" } ], "ptm_annotation": [], "interactions": [], "reactions": [], "reaction_participants": [], "reaction_enzymes": [], "biomarkers": [], "synthesized_glycans": [], "cluster_types": [], "sequence_features": { "n_linked_sites": [ 72, 206 ], "o_linked_sites": [], "snv_sites": [], "glycation_sites": [], "phosphorylation_sites": [], "sequon_annotation_sites": [ { "start_pos": 206, "end_pos": 208 }, { "start_pos": 72, "end_pos": 74 } ] }, "section_stats": [ { "table_id": "glycosylation", "table_stats": [ { "field": "total", "count": 2 }, { "field": "total_sites", "count": 2 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 2 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [ "glytoucan_ac", "type", "subtype", "site_category", "site_seq", "relation", "comment", "start_pos", "start_aa", "end_pos", "end_aa", "residue", "site_lbl" ] }, { "table_id": "glycosylation_reported", "table_stats": [ { "field": "total", "count": 2 }, { "field": "total_sites", "count": 2 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 2 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [ "glytoucan_ac", "type", "subtype", "site_category", "site_seq", "relation", "comment", "start_pos", "start_aa", "end_pos", "end_aa", "residue", "site_lbl" ] }, { "table_id": "glycosylation_reported_with_glycan", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_predicted", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "glycosylation_automatic_literature_mining", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": "n_linked_glycan_sites", "count": 0 }, { "field": "o_linked_glycans", "count": 0 }, { "field": "o_linked_glycan_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "phosphorylation", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "snv", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "snv_disease", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "snv_non_disease", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 } ], "sort_fields": [] }, { "table_id": "publication", "table_stats": [ { "field": "total", "count": 4 } ], "sort_fields": [ "title", "journal", "date", "authors" ] } ] }