{
    "uniprot_canonical_ac": "P0DPD7-4",
    "uniprot_ac": "P0DPD7",
    "uniprot_id": "EFMT4_HUMAN",
    "mass": {
        "chemical_mass": 28306.0,
        "monoisotopic_mass": 28306.0
    },
    "refseq": {
        "ac": "NP_115707",
        "name": "EEF1A lysine methyltransferase 4",
        "summary": "This gene encodes a member of the lysine-specific methyltransferase (KMT) family. The encoded enzyme catalyzes the methylation of lysine-36 of the eukaryotic translation elongation factor 1 alpha. Methylation by this enzyme may affect endoplasmic reticulum-related processes. [provided by RefSeq, Jul 2017].",
        "url": "https://www.ncbi.nlm.nih.gov/protein/NP_115707"
    },
    "gene_names": [
        {
            "name": "EEF1AKMT4",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "https://www.uniprot.org/uniprot/P0DPD7",
            "id": "P0DPD7"
        },
        {
            "name": "EEF1AKMT4",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_115707",
            "id": "NP_115707"
        }
    ],
    "protein_names": [
        {
            "name": "EEF1A lysine methyltransferase 4",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "",
            "id": "P0DPD7"
        },
        {
            "name": "2.1.1.-",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "",
            "id": "P0DPD7"
        },
        {
            "name": "EEF1A lysine methyltransferase 4",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_115707",
            "id": "NP_115707"
        },
        {
            "name": "eukaryotic translation elongation factor 1 alpha lysine specific methyltransferase 4",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_115707",
            "id": "NP_115707"
        }
    ],
    "function": [
        {
            "annotation": "Methylation of lysine (K36) in eukaryotic elongation factor alpha (eEF1A) proteins is dependent on EEF1A lysine methyltransferase 4 (eEF1A-KMT4) in vivo.",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_115707",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_115707"
                },
                {
                    "database": "PubMed",
                    "id": "28520920",
                    "url": "https://glygen.org/publication/PubMed/28520920"
                }
            ]
        },
        {
            "annotation": "Protein-lysine methyltransferase that efficiently catalyzes three successive methylations on 'Lys-36' in eukaryotic translation elongation factor 1 alpha (EEF1A1 or EEF1A2).",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P0DPD7",
                    "url": "https://www.uniprot.org/uniprot/P0DPD7#function"
                },
                {
                    "database": "PubMed",
                    "id": "28520920",
                    "url": "https://glygen.org/publication/PubMed/28520920"
                }
            ]
        },
        {
            "annotation": "This gene encodes a member of the lysine-specific methyltransferase (KMT) family. The encoded enzyme catalyzes the methylation of lysine-36 of the eukaryotic translation elongation factor 1 alpha. Methylation by this enzyme may affect endoplasmic reticulum-related processes. [provided by RefSeq, Jul 2017].",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_115707",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_115707"
                }
            ]
        }
    ],
    "disease": [],
    "snv": [],
    "mutagenesis": [
        {
            "start_pos": 129,
            "end_pos": 129,
            "sequence_org": "E",
            "sequence_mut": "A",
            "comment": "Abolishes protein-lysine N-methyltransferase activity.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P0DPD7",
                    "url": "https://www.uniprot.org/uniprot/P0DPD7"
                },
                {
                    "database": "PubMed",
                    "id": "28520920",
                    "url": "https://glygen.org/publication/PubMed/28520920"
                }
            ]
        },
        {
            "start_pos": 130,
            "end_pos": 130,
            "sequence_org": "K",
            "sequence_mut": "A",
            "comment": "Abolishes protein-lysine N-methyltransferase activity.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P0DPD7",
                    "url": "https://www.uniprot.org/uniprot/P0DPD7"
                },
                {
                    "database": "PubMed",
                    "id": "28520920",
                    "url": "https://glygen.org/publication/PubMed/28520920"
                }
            ]
        },
        {
            "start_pos": 132,
            "end_pos": 132,
            "sequence_org": "T",
            "sequence_mut": "A",
            "comment": "Reduces protein-lysine N-methyltransferase activity.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P0DPD7",
                    "url": "https://www.uniprot.org/uniprot/P0DPD7"
                },
                {
                    "database": "PubMed",
                    "id": "28520920",
                    "url": "https://glygen.org/publication/PubMed/28520920"
                }
            ]
        },
        {
            "start_pos": 133,
            "end_pos": 133,
            "sequence_org": "L",
            "sequence_mut": "A",
            "comment": "Reduces protein-lysine N-methyltransferase activity.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P0DPD7",
                    "url": "https://www.uniprot.org/uniprot/P0DPD7"
                },
                {
                    "database": "PubMed",
                    "id": "28520920",
                    "url": "https://glygen.org/publication/PubMed/28520920"
                }
            ]
        },
        {
            "start_pos": 134,
            "end_pos": 134,
            "sequence_org": "D",
            "sequence_mut": "A",
            "comment": "Abolishes protein-lysine N-methyltransferase activity.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P0DPD7",
                    "url": "https://www.uniprot.org/uniprot/P0DPD7"
                },
                {
                    "database": "PubMed",
                    "id": "28520920",
                    "url": "https://glygen.org/publication/PubMed/28520920"
                }
            ]
        },
        {
            "start_pos": 88,
            "end_pos": 88,
            "sequence_org": "D",
            "sequence_mut": "A",
            "comment": "Abolishes methyltransferase activity.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P0DPD7",
                    "url": "https://www.uniprot.org/uniprot/P0DPD7"
                },
                {
                    "database": "PubMed",
                    "id": "28520920",
                    "url": "https://glygen.org/publication/PubMed/28520920"
                }
            ]
        }
    ],
    "expression_tissue": [],
    "expression_disease": [],
    "species": [
        {
            "name": "Homo sapiens",
            "taxid": 9606,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P0DPD7",
                    "url": "https://www.uniprot.org/uniprot/P0DPD7"
                }
            ],
            "common_name": "Human"
        }
    ],
    "gene": [
        {
            "name": "EEF1AKMT4",
            "url": "https://www.uniprot.org/uniprot/P0DPD7",
            "locus": {
                "chromosome": "3",
                "start_pos": 184258759,
                "end_pos": 184249672,
                "strand": "-",
                "evidence": [
                    {
                        "database": "Ensembl Gene",
                        "id": "ENSG00000284753",
                        "url": "https://www.ensembl.org/id/ENSG00000284753"
                    }
                ]
            }
        }
    ],
    "crossref": [
        {
            "id": "ENSG00000284753",
            "url": "https://bgee.org/?page=gene&gene_id=ENSG00000284753",
            "database": "Bgee",
            "categories": [
                "Gene Expression"
            ]
        },
        {
            "id": "cd02440",
            "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd02440",
            "database": "CDD",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "EEF1AKMT4",
            "url": "http://www.genecards.org/cgi-bin/carddisp.pl?gene=EEF1AKMT4",
            "database": "GeneCards",
            "categories": [
                "Gene Annotation",
                "Organism-specific"
            ]
        },
        {
            "id": "110599564",
            "url": "https://www.ncbi.nlm.nih.gov/gene/110599564",
            "database": "GeneID",
            "categories": [
                "Genome Annotation"
            ]
        },
        {
            "id": "53611",
            "url": "https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=53611",
            "database": "HGNC",
            "categories": [
                "Organism-specific"
            ]
        },
        {
            "id": "IPR013216",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR013216",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR029063",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR029063",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "hsa:110599564",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+hsa:110599564",
            "database": "KEGG Pathway",
            "categories": [
                "Enzyme and Pathway"
            ]
        },
        {
            "id": "610145",
            "url": "http://www.omim.org/entry/610145",
            "database": "MIM",
            "categories": [
                "Other"
            ]
        },
        {
            "id": "NX_P0DPD7",
            "url": "https://www.nextprot.org/entry/NX_P0DPD7",
            "database": "neXtProt",
            "categories": [
                "Protein",
                "Organism-specific"
            ]
        },
        {
            "id": "P0DPD7",
            "url": "https://www.oglcnac.mcw.edu/search/?query_protein=P0DPD7",
            "database": "O-GlcNAc Database",
            "categories": [
                "Glycoproteomics"
            ]
        },
        {
            "id": "HYFLYVM",
            "url": "https://omabrowser.org/oma/group/HYFLYVM/",
            "database": "OMA",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "202716at2759",
            "url": "http://www.orthodb.org/?query=202716at2759",
            "database": "OrthoDB",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR12176",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR12176",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR12176:SF80",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR12176:SF80",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "2PXX",
            "url": "https://www.rcsb.org/structure/2PXX",
            "database": "PDB",
            "categories": [
                "3D Structure"
            ]
        },
        {
            "id": "PF08241",
            "url": "http://pfam.xfam.org/family/PF08241",
            "database": "Pfam",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PR_P0DPD7",
            "url": "http://purl.obolibrary.org/obo/PR_P0DPD7",
            "database": "PRO",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "NP_115707",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_115707",
            "database": "RefSeq",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "51736",
            "url": "https://www.rhea-db.org/rhea/51736",
            "database": "Rhea",
            "categories": [
                "Enzyme and Pathway"
            ]
        },
        {
            "id": "54196",
            "url": "https://www.rhea-db.org/rhea/54196",
            "database": "Rhea",
            "categories": [
                "Enzyme and Pathway"
            ]
        },
        {
            "id": "54200",
            "url": "https://www.rhea-db.org/rhea/54200",
            "database": "Rhea",
            "categories": [
                "Enzyme and Pathway"
            ]
        },
        {
            "id": "P0DPD7",
            "url": "https://www.uniprot.org/uniprot/P0DPD7",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "P0DPD7-4",
            "url": "https://www.uniprot.org/uniprot/P0DPD7-4",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        }
    ],
    "sequence": {
        "sequence": "MASPGAGRAPPELPERNCGYREVEYWDQRYQGAADSAPYDWFGDFSSFRALLEPELRPEDRILVLGCGNSALSYELFLGGFPNVTSVDYSSVVVAAMQARHAHVPQLRWETMDVRKLDFPSASFDVVLEKGTLDALLAGERDPWTVSSEGVHTVDQVLSEVSRVLVPGGRFISMTSAAPHFRTRHYAQAYYGWSLRHATYGSGFHFHLYLMHKGGKLSVAQLALGAQILSPPRPPTSPCFLQDSDHEDFLSAIQL",
        "length": 255,
        "header": "sp|P0DPD7-4|EFMT4_HUMAN Isoform 4 of EEF1A lysine methyltransferase 4  OS=Homo sapiens OX=9606 GN=EEF1AKMT4 PE=1 SV=1 CANONICAL=P0DPD7-4"
    },
    "isoforms": [
        {
            "isoform_ac": "P0DPD7-4",
            "url": "",
            "sequence": {
                "sequence": "MASPGAGRAPPELPERNCGYREVEYWDQRYQGAADSAPYDWFGDFSSFRALLEPELRPEDRILVLGCGNSALSYELFLGGFPNVTSVDYSSVVVAAMQARHAHVPQLRWETMDVRKLDFPSASFDVVLEKGTLDALLAGERDPWTVSSEGVHTVDQVLSEVSRVLVPGGRFISMTSAAPHFRTRHYAQAYYGWSLRHATYGSGFHFHLYLMHKGGKLSVAQLALGAQILSPPRPPTSPCFLQDSDHEDFLSAIQL",
                "length": 255,
                "header": "sp|P0DPD7-4|EFMT4_HUMAN Isoform 4 of EEF1A lysine methyltransferase 4  OS=Homo sapiens OX=9606 GN=EEF1AKMT4 PE=1 SV=1 CANONICAL=P0DPD7-4"
            },
            "locus": {
                "chromosome": "3",
                "start_pos": 184258572,
                "end_pos": 184249695,
                "strand": "-",
                "evidence": [
                    {
                        "database": "Ensembl Transcript",
                        "id": "ENST00000324557.9",
                        "url": "https://www.ensembl.org/id/ENST00000324557.9"
                    },
                    {
                        "database": "Ensembl Peptide",
                        "id": "ENSP00000314295.5",
                        "url": "https://www.ensembl.org/id/ENSP00000314295.5"
                    }
                ]
            }
        }
    ],
    "glycosylation": [
        {
            "glytoucan_ac": "G49108TO",
            "type": "O-linked",
            "subtype": "O-GlcNAcylation",
            "site_category": "reported_with_glycan",
            "site_seq": "",
            "relation": "attached",
            "comment": "O-GlcNAcylation",
            "evidence": [
                {
                    "id": "P0DPD7",
                    "database": "O-GlcNAc Database",
                    "url": "https://www.oglcnac.mcw.edu/search/?query_protein=P0DPD7"
                },
                {
                    "id": "28510447",
                    "database": "PubMed",
                    "url": "https://glygen.org/publication/PubMed/28510447"
                }
            ]
        }
    ],
    "phosphorylation": [
        {
            "start_pos": 39,
            "end_pos": 39,
            "kinase_uniprot_canonical_ac": "",
            "kinase_gene_name": "",
            "residue": "Tyr",
            "comment": "Phosphotyrosine",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P0DPD7",
                    "url": "https://www.uniprot.org/uniprot/P0DPD7"
                },
                {
                    "database": "PubMed",
                    "id": "15592455",
                    "url": "https://glygen.org/publication/PubMed/15592455"
                },
                {
                    "database": "iPTMnet",
                    "id": "P0DPD7",
                    "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P0DPD7"
                }
            ],
            "site_lbl": "Tyr39"
        }
    ],
    "glycation": [],
    "keywords": [
        "enzyme",
        "glycoprotein",
        "glycoprotein_reported_with_glycan"
    ],
    "publication": [
        {
            "title": "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.",
            "journal": "Nature biotechnology",
            "date": "2005",
            "authors": "Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P0DPD7",
                    "url": "https://www.uniprot.org/uniprot/P0DPD7"
                },
                {
                    "database": "iPTMnet",
                    "id": "P0DPD7-4",
                    "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P0DPD7-4"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "15592455",
                    "url": "https://glygen.org/publication/PubMed/15592455"
                }
            ]
        },
        {
            "title": "An intact SAM-dependent methyltransferase fold is encoded by the human endothelin-converting enzyme-2 gene.",
            "journal": "Proteins",
            "date": "2009",
            "authors": "Tempel W, Wu H, Dombrovsky L, Zeng H, Loppnau P, Zhu H, Plotnikov AN, Bochkarev A",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P0DPD7",
                    "url": "https://www.uniprot.org/uniprot/P0DPD7"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "19089949",
                    "url": "https://glygen.org/publication/PubMed/19089949"
                }
            ]
        },
        {
            "title": "Synthesis of a Highly Azide-Reactive and Thermosensitive Biofunctional Reagent for Efficient Enrichment and Large-Scale Identification of O-GlcNAc Proteins by Mass Spectrometry.",
            "journal": "Analytical chemistry",
            "date": "2017",
            "authors": "Zhang W, Liu T, Dong H, Bai H, Tian F, Shi Z, Chen M, Wang J, Qin W, Qian X",
            "evidence": [
                {
                    "database": "O-GlcNAc Database",
                    "id": "P0DPD7",
                    "url": "https://www.oglcnac.mcw.edu/search/?query_protein=P0DPD7"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "28510447",
                    "url": "https://glygen.org/publication/PubMed/28510447"
                }
            ]
        },
        {
            "title": "Methylation of human eukaryotic elongation factor alpha (eEF1A) by a member of a novel protein lysine methyltransferase family modulates mRNA translation.",
            "journal": "Nucleic acids research",
            "date": "2017",
            "authors": "Jakobsson ME, Malecki J, Nilges BS, Moen A, Leidel SA, Falnes PO",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "P0DPD7",
                    "url": "https://www.uniprot.org/uniprot/P0DPD7"
                },
                {
                    "database": "RefSeq",
                    "id": "NP_115707",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_115707"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "28520920",
                    "url": "https://glygen.org/publication/PubMed/28520920"
                }
            ]
        }
    ],
    "pathway": [
        {
            "id": "hsa:110599564",
            "name": "",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+hsa:110599564",
            "resource": "KEGG Pathway"
        }
    ],
    "orthologs": [
        {
            "uniprot_canonical_ac": "D4ADU7-1",
            "protein_name": "EEF1A lysine methyltransferase 4",
            "gene_name": "Eef1akmt4",
            "tax_id": 10116,
            "organism": "Rattus norvegicus",
            "sequence": {
                "sequence": "MASPRTPVSPPELPERNLQYRQVQYWDQRYKDAADSGPYEWFGDFASFRALLEPELCPEDRILVLGCGNSALSYELFLGGFPNVTSVDYSPVVVAAMQVRYAHVPSLRWETMDIRALDFPSGSFDVVLEKGTLDALLAGEPDPWNVSSEGVHTVDQVLSEVSRVLVPGGRFISMTPAGPHFRIRHYAQPCYDWSLRHTTYSSGFHFHFYVMHKGRELTVSQLALGAQILSPPSPPAPPCFLQDSDNEDFLSAIQL",
                "length": 255
            },
            "evidence": [
                {
                    "id": "P0DPD7",
                    "url": "https://omabrowser.org/oma/group/P0DPD7/",
                    "database": "OMA"
                }
            ],
            "common_name": "Rat"
        },
        {
            "uniprot_canonical_ac": "P0DPE0-1",
            "protein_name": "EEF1A lysine methyltransferase 4",
            "gene_name": "Eef1akmt4",
            "tax_id": 10090,
            "organism": "Mus musculus",
            "sequence": {
                "sequence": "MASPRTPVSPPELPEKNFQYRQVQYWDQRYKDAADSGPYEWFGDFASFRALLEPELCPEDRILVLGCGNSALSYELFLGGFPNVTSVDYSPVVVAAMQVRYAHVPSLRWETMDVRALDFPSGSFDVVLEKGTLDAMLAGEPDPWNVSSEGVHTVDQVLSEVSRLLVPGGRFISMTSAGPHFRIRHYAQSRYDWSLRHTTYSSGFHFHFYIMHKGRALSVSQLALGAQILSSPSPPASPCFLQDSDNEDFLSAIQL",
                "length": 255
            },
            "evidence": [
                {
                    "id": "45927043",
                    "url": "http://www.informatics.jax.org/homology/45927043",
                    "database": "MGI"
                },
                {
                    "id": "P0DPD7",
                    "url": "https://omabrowser.org/oma/group/P0DPD7/",
                    "database": "OMA"
                }
            ],
            "common_name": "Mouse"
        }
    ],
    "go_annotation": {
        "categories": [
            {
                "name": "Molecular Function",
                "total": 2,
                "go_terms": [
                    {
                        "name": "methyltransferase activity",
                        "id": "GO:0008168",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0008168",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "P0DPD7",
                                "url": "https://www.uniprot.org/uniprot/P0DPD7"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "protein-lysine N-methyltransferase activity",
                        "id": "GO:0016279",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016279",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "P0DPD7",
                                "url": "https://www.uniprot.org/uniprot/P0DPD7"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            },
            {
                "name": "Biological Process",
                "total": 1,
                "go_terms": [
                    {
                        "name": "methylation",
                        "id": "GO:0032259",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0032259",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "P0DPD7",
                                "url": "https://www.uniprot.org/uniprot/P0DPD7"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            }
        ]
    },
    "pro_annotation": [],
    "site_annotation": [
        {
            "start_pos": 83,
            "end_pos": 85,
            "annotation": "n_glycosylation_sequon"
        }
    ],
    "ptm_annotation": [],
    "interactions": [],
    "reactions": [
        {
            "id": "51736",
            "name": "",
            "equation": "L-lysyl-[protein] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine",
            "summary": "",
            "cellular_location": "",
            "input_participant_list": [],
            "output_participant_list": [],
            "enzyme_list": [
                "P0C6Y7-1",
                "D3ZSK5-1",
                "Q7M6Z3-1",
                "A0A0G2JTW4-1",
                "A0A0G2JW37-1",
                "D3ZEB9-1",
                "M0R767-1",
                "D4ADE5-1",
                "A0A8I6AL52-1",
                "Q8N6R0-5",
                "Q9NQV7-1",
                "Q9BYW2-1",
                "P0DPD7-4",
                "Q9NQR1-1",
                "Q9NRG4-1",
                "Q96AZ1-1",
                "Q8TBK2-1",
                "Q8WTS6-1",
                "Q5VZV1-1",
                "Q91YR5-3",
                "Q96EQ9-1",
                "E9Q5F9-1",
                "P0DPE0-1",
                "D3YWP0-1",
                "Q9CWY3-1",
                "Q2YDW7-1",
                "Q8R5A0-1",
                "Q8VHL1-1",
                "Q8BLU2-1",
                "Q54J80-1",
                "Q9VIK9-1"
            ],
            "evidence": [
                {
                    "id": "51736",
                    "database": "Rhea",
                    "url": "https://www.rhea-db.org/rhea/51736"
                }
            ]
        },
        {
            "id": "54196",
            "name": "",
            "equation": "N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-methionine = H(+) + N(6),N(6)-dimethyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine",
            "summary": "",
            "cellular_location": "",
            "input_participant_list": [],
            "output_participant_list": [],
            "enzyme_list": [
                "P0C6Y7-1",
                "Q8N6R0-5",
                "Q9NQV7-1",
                "P0DPD7-4",
                "Q96AZ1-1",
                "Q5VZV1-1",
                "Q91YR5-3",
                "P0DPE0-1",
                "D3YWP0-1",
                "Q8BLU2-1",
                "Q9VIK9-1"
            ],
            "evidence": [
                {
                    "id": "54196",
                    "database": "Rhea",
                    "url": "https://www.rhea-db.org/rhea/54196"
                }
            ]
        },
        {
            "id": "54200",
            "name": "",
            "equation": "N(6),N(6)-dimethyl-L-lysyl-[protein] + S-adenosyl-L-methionine = H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine",
            "summary": "",
            "cellular_location": "",
            "input_participant_list": [],
            "output_participant_list": [],
            "enzyme_list": [
                "P0DPD7-4",
                "Q96AZ1-1",
                "Q5VZV1-1",
                "Q96EQ9-1",
                "P0DPE0-1",
                "D3YWP0-1",
                "Q8BLU2-1"
            ],
            "evidence": [
                {
                    "id": "54200",
                    "database": "Rhea",
                    "url": "https://www.rhea-db.org/rhea/54200"
                }
            ]
        }
    ],
    "reaction_participants": [],
    "reaction_enzymes": [
        {
            "uniprot_ac": "A0A0G2JTW4",
            "gene_name": "AABR07010705.1",
            "activity": [
                {
                    "ec_number": "2.1.1.361",
                    "activity": "L-lysyl(20)-[histone H4] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "A0A0G2JTW4",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/A0A0G2JTW4"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.361",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "A0A0G2JTW4",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/A0A0G2JTW4"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "A0A0G2JW37",
            "gene_name": "RGD1561318",
            "activity": [
                {
                    "ec_number": "2.1.1.361",
                    "activity": "L-lysyl(20)-[histone H4] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "A0A0G2JW37",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/A0A0G2JW37"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.361",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "A0A0G2JW37",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/A0A0G2JW37"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "A0A8I6AL52",
            "gene_name": "ENSRNOG00000067141",
            "activity": [
                {
                    "ec_number": "2.1.1.361",
                    "activity": "L-lysyl(20)-[histone H4] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "A0A8I6AL52",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/A0A8I6AL52"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.361",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "A0A8I6AL52",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/A0A8I6AL52"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "D3YWP0",
            "gene_name": "Eef1akmt3",
            "activity": [
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "D3YWP0",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/D3YWP0"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "D3ZEB9",
            "gene_name": "Kmt5a",
            "activity": [
                {
                    "ec_number": "2.1.1.361",
                    "activity": "L-lysyl(20)-[histone H4] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "D3ZEB9",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/D3ZEB9"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.361",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "D3ZEB9",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/D3ZEB9"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "D3ZSK5",
            "gene_name": "Setd6",
            "activity": [
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "D3ZSK5",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/D3ZSK5"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "D4ADE5",
            "gene_name": "Setd7",
            "activity": [
                {
                    "ec_number": "2.1.1.364",
                    "activity": "L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "D4ADE5",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/D4ADE5"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.364",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "D4ADE5",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/D4ADE5"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "D4ADE5",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/D4ADE5"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "E9Q5F9",
            "gene_name": "Setd2",
            "activity": [
                {
                    "ec_number": "2.1.1.359",
                    "activity": "L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "E9Q5F9",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/E9Q5F9"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "E9Q5F9",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/E9Q5F9"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.359",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "E9Q5F9",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/E9Q5F9"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "M0R767",
            "gene_name": "AABR07033047.1",
            "activity": [
                {
                    "ec_number": "2.1.1.361",
                    "activity": "L-lysyl(20)-[histone H4] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "M0R767",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/M0R767"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.361",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "M0R767",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/M0R767"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "P0C6Y7",
            "gene_name": "Prdm9",
            "activity": [
                {
                    "ec_number": "2.1.1.359",
                    "activity": "L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "P0C6Y7",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/P0C6Y7"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "P0C6Y7",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/P0C6Y7"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.355",
                    "activity": "L-lysyl(9)-[histone H3] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 3 S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "P0C6Y7",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/P0C6Y7"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.354",
                    "activity": "L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "P0C6Y7",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/P0C6Y7"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.362",
                    "activity": "N(6)-methyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-methionine = H(+) + N(6),N(6)-dimethyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "P0C6Y7",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/P0C6Y7"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.361",
                    "activity": "L-lysyl(20)-[histone H4] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "P0C6Y7",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/P0C6Y7"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "P0DPD7",
            "gene_name": "EEF1AKMT4",
            "activity": [
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "P0DPD7",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/P0DPD7"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "P0DPE0",
            "gene_name": "Eef1akmt4",
            "activity": [
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "P0DPE0",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/P0DPE0"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q2YDW7",
            "gene_name": "Kmt5a",
            "activity": [
                {
                    "ec_number": "2.1.1.361",
                    "activity": "L-lysyl(20)-[histone H4] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "Q2YDW7",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q2YDW7"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.361",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q2YDW7",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q2YDW7"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q2YDW7",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q2YDW7"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q54J80",
            "gene_name": "",
            "activity": [
                {
                    "ec_number": "2.1.1.364",
                    "activity": "L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "Q54J80",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q54J80"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.364",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q54J80",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q54J80"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q5VZV1",
            "gene_name": "METTL21C",
            "activity": [
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q5VZV1",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q5VZV1"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q7M6Z3",
            "gene_name": "Smyd2",
            "activity": [
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q7M6Z3",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q7M6Z3"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.354",
                    "activity": "L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "Q7M6Z3",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q7M6Z3"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q8BLU2",
            "gene_name": "Mettl21c",
            "activity": [
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q8BLU2",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q8BLU2"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q8N6R0",
            "gene_name": "METTL13",
            "activity": [
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q8N6R0",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q8N6R0"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q8R5A0",
            "gene_name": "Smyd2",
            "activity": [
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q8R5A0",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q8R5A0"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.354",
                    "activity": "L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "Q8R5A0",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q8R5A0"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q8TBK2",
            "gene_name": "SETD6",
            "activity": [
                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q8TBK2",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q8TBK2"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q8VHL1",
            "gene_name": "Setd7",
            "activity": [
                {
                    "ec_number": "2.1.1.364",
                    "activity": "L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "Q8VHL1",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q8VHL1"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.364",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q8VHL1",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q8VHL1"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q8WTS6",
            "gene_name": "SETD7",
            "activity": [
                {
                    "ec_number": "2.1.1.364",
                    "activity": "L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "Q8WTS6",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q8WTS6"
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                    ]
                },
                {
                    "ec_number": "2.1.1.364",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q8WTS6",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q8WTS6"
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                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q91YR5",
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                {
                    "ec_number": "2.1.1.-",
                    "activity": "",
                    "evidence": [
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                            "id": "Q91YR5",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q91YR5"
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                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q96AZ1",
            "gene_name": "EEF1AKMT3",
            "activity": [
                {
                    "ec_number": "2.1.1.-",
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                    "evidence": [
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                            "id": "Q96AZ1",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q96AZ1"
                        }
                    ]
                }
            ]
        },
        {
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            "activity": [
                {
                    "ec_number": "2.1.1.43",
                    "activity": "",
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                        {
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                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q96EQ9"
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                    ]
                },
                {
                    "ec_number": "2.1.1.359",
                    "activity": "L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
                            "id": "Q96EQ9",
                            "database": "UniProtKB",
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                    ]
                },
                {
                    "ec_number": "2.1.1.-",
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                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q96EQ9"
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                    ]
                },
                {
                    "ec_number": "2.1.1.355",
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                    "evidence": [
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                    ]
                },
                {
                    "ec_number": "2.1.1.354",
                    "activity": "L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine.",
                    "evidence": [
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                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q96EQ9"
                        }
                    ]
                },
                {
                    "ec_number": "2.1.1.362",
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                    "evidence": [
                        {
                            "id": "Q96EQ9",
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                            "url": "https://www.uniprot.org/uniprot/Q96EQ9"
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                    ]
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                {
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                    ]
                }
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        {
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                {
                    "ec_number": "2.1.1.359",
                    "activity": "L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine.",
                    "evidence": [
                        {
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                            "database": "UniProtKB",
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                {
                    "ec_number": "2.1.1.359",
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                    ]
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                {
                    "ec_number": "2.1.1.-",
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                            "database": "UniProtKB",
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                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q9CWY3",
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                {
                    "ec_number": "2.1.1.-",
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        {
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                {
                    "ec_number": "2.1.1.-",
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                {
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                {
                    "ec_number": "2.1.1.359",
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    "biomarkers": [],
    "synthesized_glycans": [],
    "cluster_types": [
        {
            "name": "homologset.oma",
            "label": "OMA Homologs"
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        {
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    "sequence_features": {
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        "glycation_sites": [],
        "phosphorylation_sites": [
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