{
    "uniprot_canonical_ac": "Q9QWV4-1",
    "uniprot_ac": "Q9QWV4",
    "uniprot_id": "MLF1_MOUSE",
    "mass": {
        "chemical_mass": 30432.0,
        "monoisotopic_mass": 30432.0
    },
    "refseq": {
        "ac": "NP_034931",
        "name": "myeloid leukemia factor 1 isoform b",
        "summary": "",
        "url": "https://www.ncbi.nlm.nih.gov/protein/NP_034931"
    },
    "gene_names": [
        {
            "name": "Mlf1",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "https://www.uniprot.org/uniprot/Q9QWV4",
            "id": "Q9QWV4"
        },
        {
            "name": "HLS7",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001034632",
            "id": "NP_001034632"
        },
        {
            "name": "Hls7",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "https://www.uniprot.org/uniprot/Q9QWV4",
            "id": "Q9QWV4"
        },
        {
            "name": "Mlf1",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001034632",
            "id": "NP_001034632"
        }
    ],
    "protein_names": [
        {
            "name": "Myeloid leukemia factor 1",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "",
            "id": "Q9QWV4"
        },
        {
            "name": "Hematopoietic lineage switch 7",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "",
            "id": "Q9QWV4"
        },
        {
            "name": "Myelodysplasia-myeloid leukemia factor 1",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "",
            "id": "Q9QWV4"
        },
        {
            "name": "hematopoietic lineage switch 7; myelodysplasia-myeloid leukemia factor 1",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_034931",
            "id": "NP_034931"
        },
        {
            "name": "myeloid leukemia factor 1 isoform a",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001034632",
            "id": "NP_001034632"
        },
        {
            "name": "myeloid leukemia factor 1 isoform b",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_034931",
            "id": "NP_034931"
        }
    ],
    "function": [
        {
            "annotation": "Ectopically expressed MLF1 could be responsible for the pathological apoptosis in early myelodysplastic syndrome (MDS) patients.",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_034931",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_034931"
                },
                {
                    "database": "PubMed",
                    "id": "26563351",
                    "url": "https://glygen.org/publication/PubMed/26563351"
                },
                {
                    "database": "RefSeq",
                    "id": "NP_001034632",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001034632"
                }
            ]
        },
        {
            "annotation": "Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4#function"
                },
                {
                    "database": "PubMed",
                    "id": "10523300",
                    "url": "https://glygen.org/publication/PubMed/10523300"
                },
                {
                    "database": "PubMed",
                    "id": "15122318",
                    "url": "https://glygen.org/publication/PubMed/15122318"
                }
            ]
        },
        {
            "annotation": "The data indicate that MLF1 serves as a proapoptotic antagonist that interacts with the HAX1/HtrA2-OMI/PARL (HOP) mitochondrial complex to modulate cell survival.",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_034931",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_034931"
                },
                {
                    "database": "PubMed",
                    "id": "28137643",
                    "url": "https://glygen.org/publication/PubMed/28137643"
                },
                {
                    "database": "RefSeq",
                    "id": "NP_001034632",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001034632"
                }
            ]
        }
    ],
    "disease": [],
    "snv": [],
    "mutagenesis": [
        {
            "start_pos": 149,
            "end_pos": 149,
            "sequence_org": "S",
            "sequence_mut": "A",
            "comment": "No effect on binding to YWHAZ.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4"
                },
                {
                    "database": "PubMed",
                    "id": "17008314",
                    "url": "https://glygen.org/publication/PubMed/17008314"
                }
            ]
        },
        {
            "start_pos": 34,
            "end_pos": 34,
            "sequence_org": "S",
            "sequence_mut": "A",
            "comment": "70% reduction in binding to YWHAZ and increased nuclear localization.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4"
                },
                {
                    "database": "PubMed",
                    "id": "17008314",
                    "url": "https://glygen.org/publication/PubMed/17008314"
                }
            ]
        }
    ],
    "expression_tissue": [],
    "expression_disease": [],
    "species": [
        {
            "name": "Mus musculus",
            "taxid": 10090,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4"
                }
            ],
            "common_name": "Mouse"
        }
    ],
    "gene": [
        {
            "name": "Mlf1",
            "url": "https://www.uniprot.org/uniprot/Q9QWV4",
            "locus": {
                "chromosome": "3",
                "start_pos": 67307336,
                "end_pos": 67281430,
                "strand": "-",
                "evidence": [
                    {
                        "database": "Ensembl Gene",
                        "id": "ENSMUSG00000048416",
                        "url": "https://www.ensembl.org/id/ENSMUSG00000048416"
                    }
                ]
            }
        }
    ],
    "crossref": [
        {
            "id": "ENSMUSG00000048416",
            "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000048416",
            "database": "Bgee",
            "categories": [
                "Gene Expression"
            ]
        },
        {
            "id": "17349",
            "url": "https://www.ncbi.nlm.nih.gov/gene/17349",
            "database": "GeneID",
            "categories": [
                "Genome Annotation"
            ]
        },
        {
            "id": "Q9QWV4",
            "url": "https://www.ebi.ac.uk/intact/interactors/id:Q9QWV4*",
            "database": "IntAct",
            "categories": [
                "Other"
            ]
        },
        {
            "id": "IPR019376",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR019376",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "mmu:17349",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:17349",
            "database": "KEGG Pathway",
            "categories": [
                "Enzyme and Pathway"
            ]
        },
        {
            "id": "1341819",
            "url": "http://www.informatics.jax.org/marker/MGI:1341819",
            "database": "MGI",
            "categories": [
                "Organism-specific"
            ]
        },
        {
            "id": "FINMNET",
            "url": "https://omabrowser.org/oma/group/FINMNET/",
            "database": "OMA",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "3678235at2759",
            "url": "http://www.orthodb.org/?query=3678235at2759",
            "database": "OrthoDB",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR13105",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR13105",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR13105:SF7",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR13105:SF7",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PF10248",
            "url": "http://pfam.xfam.org/family/PF10248",
            "database": "Pfam",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PR_Q9QWV4",
            "url": "http://purl.obolibrary.org/obo/PR_Q9QWV4",
            "database": "PRO",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "NP_034931",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_034931",
            "database": "RefSeq",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "A0A0A6YVP7",
            "url": "https://www.uniprot.org/uniprot/A0A0A6YVP7",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "A0A0A6YVP7-1",
            "url": "https://www.uniprot.org/uniprot/A0A0A6YVP7-1",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "Q8JZS2",
            "url": "https://www.uniprot.org/uniprot/Q8JZS2",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "Q8JZS2-1",
            "url": "https://www.uniprot.org/uniprot/Q8JZS2-1",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "Q9QWV4",
            "url": "https://www.uniprot.org/uniprot/Q9QWV4",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "Q9QWV4-1",
            "url": "https://www.uniprot.org/uniprot/Q9QWV4-1",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        }
    ],
    "sequence": {
        "sequence": "MFRMLSSSFEDDPFFADSFLAHRESMRNMMRSFSEPLGRDLLSISDGRGRTHNRRERDDGEDSLTHADVNPFQTMDRMMANMRSGIQELQRNFGQLSMDPNGHSFCSSSVMTYSKVGDEPPKVFQASTQTRRAPGGVKETRKAMRDSDSGLERMAVGHHIHDRGHVIRKSKNNKTGDEEVNQEFINMNESDAHAFDDEWQNEVLKYKSIGRSGNTGMRSVGHEHPGSRELKRREKIHRNSAIESGRRSNVFVDKLNVKGSPVKITKK",
        "length": 267,
        "header": "sp|Q9QWV4-1|MLF1_MOUSE Isoform 1 of Myeloid leukemia factor 1  OS=Mus musculus OX=10090 GN=Mlf1 PE=1 SV=1 CANONICAL=Q9QWV4-1"
    },
    "isoforms": [
        {
            "isoform_ac": "A0A0A6YVP7-1",
            "url": "",
            "sequence": {
                "sequence": "MFRMLSSSFEDDPFFADSFLAHRESMRNMMRSFSEPLGRDLLSISDGRGRTHNRRERDDGEDSLTKTYLHL",
                "length": 71,
                "header": "tr|A0A0A6YVP7-1|A0A0A6YVP7_MOUSE Computationally mapped isoform of Myeloid leukemia factor 1  OS=Mus musculus OX=10090 GN=Mlf1 PE=1 SV=1 CANONICAL=Q9QWV4-1"
            },
            "locus": {
                "chromosome": "3",
                "start_pos": 67300190,
                "end_pos": 67281549,
                "strand": "-",
                "evidence": [
                    {
                        "database": "Ensembl Transcript",
                        "id": "ENSMUST00000126628.7",
                        "url": "https://www.ensembl.org/id/ENSMUST00000126628.7"
                    },
                    {
                        "database": "Ensembl Peptide",
                        "id": "ENSMUSP00000141208.2",
                        "url": "https://www.ensembl.org/id/ENSMUSP00000141208.2"
                    }
                ]
            }
        },
        {
            "isoform_ac": "Q8JZS2-1",
            "url": "",
            "sequence": {
                "sequence": "MFRMLSSSFEDDPFFADSFLAHRESMRNMMRSFSEPLGRDLLSISDGRGRTHNRRERDDGEDSLTATSFSLVPFGRFGGMHADVNPFQTMDRMMANMRSGIQELQRNFGQLSMDPNGHSFCSSSVMTYSKVGDEPPKVFQASTQTRRAPGGVKETRKAMRDSDSGLERMAVGHHIHDRGHVIRKSKNNKTGDEEVNQEFINMNESDAHAFDDEWQNEVLKYKSIGRSGNTGMRSVGHEHPGSRELKRREKIHRNSAIESGRRSNVFVDKLNVKGSPVKITKK",
                "length": 282,
                "header": "tr|Q8JZS2-1|Q8JZS2_MOUSE Computationally mapped isoform of Myeloid leukemia factor 1  OS=Mus musculus OX=10090 GN=Mlf1 PE=1 SV=1 CANONICAL=Q9QWV4-1"
            },
            "locus": {
                "chromosome": "3",
                "start_pos": 67307137,
                "end_pos": 67281549,
                "strand": "-",
                "evidence": [
                    {
                        "database": "Ensembl Transcript",
                        "id": "ENSMUST00000061322.10",
                        "url": "https://www.ensembl.org/id/ENSMUST00000061322.10"
                    },
                    {
                        "database": "Ensembl Peptide",
                        "id": "ENSMUSP00000058596.9",
                        "url": "https://www.ensembl.org/id/ENSMUSP00000058596.9"
                    }
                ]
            }
        },
        {
            "isoform_ac": "Q9QWV4-1",
            "url": "",
            "sequence": {
                "sequence": "MFRMLSSSFEDDPFFADSFLAHRESMRNMMRSFSEPLGRDLLSISDGRGRTHNRRERDDGEDSLTHADVNPFQTMDRMMANMRSGIQELQRNFGQLSMDPNGHSFCSSSVMTYSKVGDEPPKVFQASTQTRRAPGGVKETRKAMRDSDSGLERMAVGHHIHDRGHVIRKSKNNKTGDEEVNQEFINMNESDAHAFDDEWQNEVLKYKSIGRSGNTGMRSVGHEHPGSRELKRREKIHRNSAIESGRRSNVFVDKLNVKGSPVKITKK",
                "length": 267,
                "header": "sp|Q9QWV4-1|MLF1_MOUSE Isoform 1 of Myeloid leukemia factor 1  OS=Mus musculus OX=10090 GN=Mlf1 PE=1 SV=1 CANONICAL=Q9QWV4-1"
            },
            "locus": {
                "chromosome": "3",
                "start_pos": 67307137,
                "end_pos": 67281549,
                "strand": "-",
                "evidence": [
                    {
                        "database": "Ensembl Transcript",
                        "id": "ENSMUST00000077916",
                        "url": "https://www.ensembl.org/id/ENSMUST00000077916"
                    },
                    {
                        "database": "Ensembl Peptide",
                        "id": "ENSMUSP00000077072",
                        "url": "https://www.ensembl.org/id/ENSMUSP00000077072"
                    }
                ]
            }
        }
    ],
    "glycosylation": [],
    "phosphorylation": [
        {
            "start_pos": 32,
            "end_pos": 32,
            "kinase_uniprot_canonical_ac": "",
            "kinase_gene_name": "",
            "residue": "Ser",
            "comment": "Phosphoserine",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4"
                }
            ],
            "site_lbl": "Ser32"
        },
        {
            "start_pos": 34,
            "end_pos": 34,
            "kinase_uniprot_canonical_ac": "",
            "kinase_gene_name": "",
            "residue": "Ser",
            "comment": "Phosphoserine",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4"
                }
            ],
            "site_lbl": "Ser34"
        },
        {
            "start_pos": 6,
            "end_pos": 6,
            "kinase_uniprot_canonical_ac": "",
            "kinase_gene_name": "",
            "residue": "Ser",
            "comment": "Phosphoserine",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4"
                },
                {
                    "database": "PubMed",
                    "id": "21183079",
                    "url": "https://glygen.org/publication/PubMed/21183079"
                },
                {
                    "database": "iPTMnet",
                    "id": "Q9QWV4",
                    "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q9QWV4"
                }
            ],
            "site_lbl": "Ser6"
        },
        {
            "start_pos": 8,
            "end_pos": 8,
            "kinase_uniprot_canonical_ac": "",
            "kinase_gene_name": "",
            "residue": "Ser",
            "comment": "Phosphoserine",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4"
                }
            ],
            "site_lbl": "Ser8"
        }
    ],
    "glycation": [],
    "keywords": [],
    "publication": [
        {
            "title": "cDNA cloning, expression pattern, and chromosomal localization of Mlf1, murine homologue of a gene involved in myelodysplasia and acute myeloid leukemia.",
            "journal": "Am. J. Pathol.",
            "date": "1999",
            "authors": "Copeland N.G., Gilbert D.J., Jenkins N.A., Look A.T., Morris S.W., Naeve C.W., Witte D.P., Hitzler J.K.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "10393836",
                    "url": "https://glygen.org/publication/PubMed/10393836"
                }
            ]
        },
        {
            "title": "HLS7, a hemopoietic lineage switch gene homologous to the leukemia-inducing gene MLF1.",
            "journal": "EMBO J.",
            "date": "1999",
            "authors": "Williams JH, Daly LN, Ingley E, Beaumont JG, Tilbrook PA, Lalonde JP, Stillitano JP, Klinken SP",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "10523300",
                    "url": "https://glygen.org/publication/PubMed/10523300"
                }
            ]
        },
        {
            "title": "MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1.",
            "journal": "J. Biol. Chem.",
            "date": "2002",
            "authors": "Lim R, Winteringham LN, Williams JH, McCulloch RK, Ingley E, Tiao JY, Lalonde JP, Tsai S, Tilbrook PA, Sun Y, Wu X, Morris SW, Klinken SP",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "12176995",
                    "url": "https://glygen.org/publication/PubMed/12176995"
                }
            ]
        },
        {
            "title": "Myeloid leukemia factor 1 inhibits erythropoietin-induced differentiation, cell cycle exit and p27Kip1 accumulation.",
            "journal": "Oncogene",
            "date": "2004",
            "authors": "Ingley E., Williams J.H., Klinken S.P., Winteringham L.N., Kobelke S.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "15122318",
                    "url": "https://glygen.org/publication/PubMed/15122318"
                }
            ]
        },
        {
            "title": "Myeloid leukemia factor 1 associates with a novel heterogeneous nuclear ribonucleoprotein U-like molecule.",
            "journal": "J. Biol. Chem.",
            "date": "2006",
            "authors": "Winteringham LN, Endersby R, Kobelke S, McCulloch RK, Williams JH, Stillitano J, Cornwall SM, Ingley E, Klinken SP",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "17008314",
                    "url": "https://glygen.org/publication/PubMed/17008314"
                }
            ]
        },
        {
            "title": "A tissue-specific atlas of mouse protein phosphorylation and expression.",
            "journal": "Cell",
            "date": "2010",
            "authors": "Huttlin EL, Jedrychowski MP, Elias JE, Goswami T, Rad R, Beausoleil SA, Villen J, Haas W, Sowa ME, Gygi SP",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9QWV4",
                    "url": "https://www.uniprot.org/uniprot/Q9QWV4"
                },
                {
                    "database": "iPTMnet",
                    "id": "Q9QWV4",
                    "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q9QWV4"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "21183079",
                    "url": "https://glygen.org/publication/PubMed/21183079"
                }
            ]
        },
        {
            "title": "Myeloid leukemia factor 1 interfered with Bcl-XL to promote apoptosis and its function was regulated by 14-3-3.",
            "journal": "Journal of physiology and biochemistry",
            "date": "2015",
            "authors": "Sun Y, Fu A, Xu W, Chao JR, Moshiach S, Morris SW",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001034632",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001034632"
                },
                {
                    "database": "RefSeq",
                    "id": "NP_034931",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_034931"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
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        {
            "title": "Identification of FOXJ1 effectors during ciliogenesis in the foetal respiratory epithelium and embryonic left-right organiser of the mouse.",
            "journal": "Dev. Biol.",
            "date": "2017",
            "authors": "Beckers A., Mai M., Gossler A., Kracht M., Stauber M., Weidemann M., Alten L., Dittrich-Breiholz O., Lobschat K.",
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                    "type": "PubMed",
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            "title": "MLF1 is a proapoptotic antagonist of HOP complex-mediated survival.",
            "journal": "Biochimica et biophysica acta. Molecular cell research",
            "date": "2017",
            "authors": "Sun Y, Chao JR, Xu W, Pourpak A, Boyd K, Moshiach S, Qi GY, Fu A, Shao HR, Pounds S, Morris SW",
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