{
    "uniprot_canonical_ac": "Q9CQG1-1",
    "uniprot_ac": "Q9CQG1",
    "uniprot_id": "CHAC2_MOUSE",
    "mass": {
        "chemical_mass": 20153.0,
        "monoisotopic_mass": 20153.0
    },
    "refseq": {
        "ac": "NP_001277596",
        "name": "putative glutathione-specific gamma-glutamylcyclotransferase 2 isoform 2",
        "summary": "",
        "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277596"
    },
    "gene_names": [
        {
            "name": "Chac2",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "https://www.uniprot.org/uniprot/Q9CQG1",
            "id": "Q9CQG1"
        },
        {
            "name": "Chac2",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277596",
            "id": "NP_001277596"
        }
    ],
    "protein_names": [
        {
            "name": "Putative glutathione-specific gamma-glutamylcyclotransferase 2",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "",
            "id": "Q9CQG1"
        },
        {
            "name": "4.3.2.7",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "",
            "id": "Q9CQG1"
        },
        {
            "name": "Cation transport regulator-like protein 2",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "",
            "id": "Q9CQG1"
        },
        {
            "name": "Gamma-GCG 2",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "",
            "id": "Q9CQG1"
        },
        {
            "name": "cation transport regulator-like protein 2; ChaC, cation transport regulator homolog 2; gamma-GCG 2; putative glutathione-specific gamma-glutamylcyclotransferase 2",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277596",
            "id": "NP_001277596"
        },
        {
            "name": "putative glutathione-specific gamma-glutamylcyclotransferase 2 isoform 2",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277596",
            "id": "NP_001277596"
        }
    ],
    "function": [
        {
            "annotation": "Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide (PubMed:27913623). Acts specifically on glutathione, but not on other gamma-glutamyl peptides (By similarity).",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9CQG1",
                    "url": "https://www.uniprot.org/uniprot/Q9CQG1#function"
                },
                {
                    "database": "UniProtKB",
                    "id": "Q8WUX2",
                    "url": "https://www.uniprot.org/uniprot/Q8WUX2#function"
                },
                {
                    "database": "PubMed",
                    "id": "27913623",
                    "url": "https://glygen.org/publication/PubMed/27913623"
                }
            ]
        },
        {
            "annotation": "Human and mouse ChaC2 proteins purified in vitro show 10-20-fold lower catalytic efficiency than ChaC1, although they showed comparable Km values",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001277596",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277596"
                },
                {
                    "database": "PubMed",
                    "id": "27913623",
                    "url": "https://glygen.org/publication/PubMed/27913623"
                }
            ]
        }
    ],
    "disease": [],
    "snv": [],
    "mutagenesis": [],
    "expression_tissue": [],
    "expression_disease": [],
    "species": [
        {
            "name": "Mus musculus",
            "taxid": 10090,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9CQG1",
                    "url": "https://www.uniprot.org/uniprot/Q9CQG1"
                }
            ],
            "common_name": "Mouse"
        }
    ],
    "gene": [
        {
            "name": "Chac2",
            "url": "https://www.uniprot.org/uniprot/Q9CQG1",
            "locus": {
                "chromosome": "11",
                "start_pos": 30926707,
                "end_pos": 30936350,
                "strand": "+",
                "evidence": [
                    {
                        "database": "Ensembl Gene",
                        "id": "ENSMUSG00000020309",
                        "url": "https://www.ensembl.org/id/ENSMUSG00000020309"
                    }
                ]
            }
        }
    ],
    "crossref": [
        {
            "id": "ENSMUSG00000020309",
            "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000020309",
            "database": "Bgee",
            "categories": [
                "Gene Expression"
            ]
        },
        {
            "id": "4.3.2.7",
            "url": "https://www.brenda-enzymes.org/enzyme.php?ecno=4.3.2.7&UniProtAcc=Q9CQG1",
            "database": "BRENDA",
            "categories": [
                "Enzyme and Pathway"
            ]
        },
        {
            "id": "cd06661",
            "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd06661",
            "database": "CDD",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "68044",
            "url": "https://www.ncbi.nlm.nih.gov/gene/68044",
            "database": "GeneID",
            "categories": [
                "Genome Annotation"
            ]
        },
        {
            "id": "IPR006840",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR006840",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR013024",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR013024",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR036568",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR036568",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "mmu:68044",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:68044",
            "database": "KEGG Pathway",
            "categories": [
                "Enzyme and Pathway"
            ]
        },
        {
            "id": "1915294",
            "url": "http://www.informatics.jax.org/marker/MGI:1915294",
            "database": "MGI",
            "categories": [
                "Organism-specific"
            ]
        },
        {
            "id": "PGYINGY",
            "url": "https://omabrowser.org/oma/group/PGYINGY/",
            "database": "OMA",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "662654at2759",
            "url": "http://www.orthodb.org/?query=662654at2759",
            "database": "OrthoDB",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR12192",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR12192",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR12192:SF2",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR12192:SF2",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PF04752",
            "url": "http://pfam.xfam.org/family/PF04752",
            "database": "Pfam",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PR_Q9CQG1",
            "url": "http://purl.obolibrary.org/obo/PR_Q9CQG1",
            "database": "PRO",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "R-MMU-174403",
            "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-174403",
            "database": "Reactome",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "NP_001277596",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277596",
            "database": "RefSeq",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "47724",
            "url": "https://www.rhea-db.org/rhea/47724",
            "database": "Rhea",
            "categories": [
                "Enzyme and Pathway"
            ]
        },
        {
            "id": "Q9CQG1",
            "url": "https://www.uniprot.org/uniprot/Q9CQG1",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "Q9CQG1-1",
            "url": "https://www.uniprot.org/uniprot/Q9CQG1-1",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "Q9CQG1-2",
            "url": "https://www.uniprot.org/uniprot/Q9CQG1-2",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        }
    ],
    "sequence": {
        "sequence": "MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPGGSVWGVAYKLPVGKEEEVKTYLDFREKGGYRTTTVIFYPKDSTTKPFSVLLYIGTCDNPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSVRKLVPEDADEHLFSLEKLVKERLEGK",
        "length": 178,
        "header": "sp|Q9CQG1-1|CHAC2_MOUSE Isoform 1 of Putative glutathione-specific gamma-glutamylcyclotransferase 2  OS=Mus musculus OX=10090 GN=Chac2 PE=1 SV=1 CANONICAL=Q9CQG1-1"
    },
    "isoforms": [
        {
            "isoform_ac": "Q9CQG1-1",
            "url": "",
            "sequence": {
                "sequence": "MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPGGSVWGVAYKLPVGKEEEVKTYLDFREKGGYRTTTVIFYPKDSTTKPFSVLLYIGTCDNPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSVRKLVPEDADEHLFSLEKLVKERLEGK",
                "length": 178,
                "header": "sp|Q9CQG1-1|CHAC2_MOUSE Isoform 1 of Putative glutathione-specific gamma-glutamylcyclotransferase 2  OS=Mus musculus OX=10090 GN=Chac2 PE=1 SV=1 CANONICAL=Q9CQG1-1"
            },
            "locus": {
                "chromosome": "11",
                "start_pos": 30936246,
                "end_pos": 30927384,
                "strand": "+",
                "evidence": [
                    {
                        "database": "Ensembl Transcript",
                        "id": "ENSMUST00000101394",
                        "url": "https://www.ensembl.org/id/ENSMUST00000101394"
                    },
                    {
                        "database": "Ensembl Peptide",
                        "id": "ENSMUSP00000098942",
                        "url": "https://www.ensembl.org/id/ENSMUSP00000098942"
                    }
                ]
            }
        },
        {
            "isoform_ac": "Q9CQG1-2",
            "url": "",
            "sequence": {
                "sequence": "MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKGSVWGVAYKLPVGKEEEVKTYLDFREKGGYRTTTVIFYPKDSTTKPFSVLLYIGTCDNPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSVRKLVPEDADEHLFSLEKLVKERLEGK",
                "length": 166,
                "header": "sp|Q9CQG1-2|CHAC2_MOUSE Isoform 2 of Putative glutathione-specific gamma-glutamylcyclotransferase 2  OS=Mus musculus OX=10090 GN=Chac2 PE=1 SV=1 CANONICAL=Q9CQG1-1"
            },
            "locus": {
                "chromosome": "11",
                "start_pos": 30936246,
                "end_pos": 30927384,
                "strand": "+",
                "evidence": [
                    {
                        "database": "Ensembl Transcript",
                        "id": "ENSMUST00000020553",
                        "url": "https://www.ensembl.org/id/ENSMUST00000020553"
                    },
                    {
                        "database": "Ensembl Peptide",
                        "id": "ENSMUSP00000020553",
                        "url": "https://www.ensembl.org/id/ENSMUSP00000020553"
                    }
                ]
            }
        }
    ],
    "glycosylation": [],
    "phosphorylation": [],
    "glycation": [],
    "keywords": [
        "enzyme"
    ],
    "publication": [
        {
            "title": "ChaC2, an enzyme for slow turnover of cytosolic glutathione.",
            "journal": "J. Biol. Chem.",
            "date": "2017",
            "authors": "Kaur A, Gautam R, Srivastava R, Chandel A, Kumar A, Karthikeyan S, Bachhawat AK",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q9CQG1",
                    "url": "https://www.uniprot.org/uniprot/Q9CQG1"
                },
                {
                    "database": "RefSeq",
                    "id": "NP_001277596",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001277596"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "27913623",
                    "url": "https://glygen.org/publication/PubMed/27913623"
                }
            ]
        }
    ],
    "pathway": [
        {
            "id": "mmu:68044",
            "name": "",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:68044",
            "resource": "KEGG Pathway"
        },
        {
            "id": "R-MMU-174403",
            "name": "Glutathione synthesis and recycling",
            "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-174403",
            "resource": "Reactome",
            "description": "Glutathione synthesis and recycling"
        }
    ],
    "orthologs": [
        {
            "uniprot_canonical_ac": "P32656-1",
            "protein_name": "Glutathione-specific gamma-glutamylcyclotransferase",
            "gene_name": "GCG1",
            "tax_id": 559292,
            "organism": "Saccharomyces cerevisiae S288C",
            "sequence": {
                "sequence": "MTNDNSGIWVLGYGSLIYKPPSHYTHRIPAIIHGFARRFWQSSTDHRGTPANPGRVATLIPYEDIIRQTAFLKNVNLYSESAPIQDPDDLVTIGVVYYIPPEHAQEVREYLNVREQNGYTLHEVEVHLETNREHEAELGEALEQLPRHNKSGKRVLLTSVYIGTIDNEAFVGPETVDETAKVIAVSHGPSGSNYEYLAKLEQALAQMPIMKERGRITDHYLTALLETVNKYR",
                "length": 232
            },
            "evidence": [
                {
                    "id": "Q9CQG1",
                    "url": "https://omabrowser.org/oma/group/Q9CQG1/",
                    "database": "OMA"
                }
            ],
            "common_name": "Yeast"
        },
        {
            "uniprot_canonical_ac": "Q641Z5-1",
            "protein_name": "Putative glutathione-specific gamma-glutamylcyclotransferase 2",
            "gene_name": "Chac2",
            "tax_id": 10116,
            "organism": "Rattus norvegicus",
            "sequence": {
                "sequence": "MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPGGSVWGVAYKLPVGKEEEVKTYLDFREKGGYRTTTVIFYPKDSTTKPFSVLLYIGTCDNPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSIRKLVPEDADEHLFSLEKLVKERLEGK",
                "length": 178
            },
            "evidence": [
                {
                    "id": "45233846",
                    "url": "http://www.informatics.jax.org/homology/45233846",
                    "database": "MGI"
                },
                {
                    "id": "Q9CQG1",
                    "url": "https://omabrowser.org/oma/group/Q9CQG1/",
                    "database": "OMA"
                }
            ],
            "common_name": "Rat"
        },
        {
            "uniprot_canonical_ac": "Q8WUX2-1",
            "protein_name": "Glutathione-specific gamma-glutamylcyclotransferase 2",
            "gene_name": "CHAC2",
            "tax_id": 9606,
            "organism": "Homo sapiens",
            "sequence": {
                "sequence": "MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPAGCVWGVAYRLPVGKEEEVKAYLDFREKGGYRTTTVIFYPKDPTTKPFSVLLYIGTCDNPDYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELANSIRNLVPEEADEHLFALEKLVKERLEGKQNLNCI",
                "length": 184
            },
            "evidence": [
                {
                    "id": "45233846",
                    "url": "http://www.informatics.jax.org/homology/45233846",
                    "database": "MGI"
                },
                {
                    "id": "Q9CQG1",
                    "url": "https://omabrowser.org/oma/group/Q9CQG1/",
                    "database": "OMA"
                }
            ],
            "common_name": "Human"
        }
    ],
    "go_annotation": {
        "categories": [
            {
                "name": "Molecular Function",
                "total": 2,
                "go_terms": [
                    {
                        "name": "gamma-glutamylcyclotransferase activity",
                        "id": "GO:0003839",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003839",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "Q9CQG1",
                                "url": "https://www.uniprot.org/uniprot/Q9CQG1"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "glutathione specific gamma-glutamylcyclotransferase activity",
                        "id": "GO:0061928",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0061928",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "Q9CQG1",
                                "url": "https://www.uniprot.org/uniprot/Q9CQG1"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            },
            {
                "name": "Biological Process",
                "total": 1,
                "go_terms": [
                    {
                        "name": "glutathione catabolic process",
                        "id": "GO:0006751",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006751",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "Q9CQG1",
                                "url": "https://www.uniprot.org/uniprot/Q9CQG1"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            },
            {
                "name": "Cellular Component",
                "total": 2,
                "go_terms": [
                    {
                        "name": "cytoplasm",
                        "id": "GO:0005737",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005737",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "Q9CQG1",
                                "url": "https://www.uniprot.org/uniprot/Q9CQG1"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "cytosol",
                        "id": "GO:0005829",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005829",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "Q9CQG1",
                                "url": "https://www.uniprot.org/uniprot/Q9CQG1"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            }
        ]
    },
    "pro_annotation": [],
    "site_annotation": [
        {
            "start_pos": 27,
            "end_pos": 29,
            "annotation": "n_glycosylation_sequon"
        }
    ],
    "ptm_annotation": [],
    "interactions": [],
    "reactions": [
        {
            "id": "47724",
            "name": "",
            "equation": "glutathione = 5-oxo-L-proline + L-cysteinylglycine",
            "summary": "",
            "cellular_location": "",
            "input_participant_list": [],
            "output_participant_list": [],
            "enzyme_list": [
                "B3STU3-1",
                "Q641Z5-1",
                "Q8WUX2-1",
                "Q9BUX1-1",
                "Q8R3J5-1",
                "Q9CQG1-1",
                "P32656-1",
                "Q54LB6-1",
                "Q9VYK7-1",
                "Q9VCI9-1"
            ],
            "evidence": [
                {
                    "id": "47724",
                    "database": "Rhea",
                    "url": "https://www.rhea-db.org/rhea/47724"
                }
            ]
        },
        {
            "id": "R-MMU-6785928",
            "name": "CHAC1,2 cleaves GSH to OPRO and CysGly",
            "equation": "",
            "summary": "This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>",
            "cellular_location": "cytosol",
            "input_participant_list": [],
            "output_participant_list": [
                "R-ALL-1247904"
            ],
            "enzyme_list": [
                "Q9CQG1-1",
                "Q8R3J5-1"
            ],
            "evidence": [
                {
                    "id": "R-MMU-6785928",
                    "database": "Reactome",
                    "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-6785928"
                }
            ]
        }
    ],
    "reaction_participants": [
        {
            "participant_id": "R-ALL-1247904",
            "participant_name": "CysGly [cytosol]",
            "crossref": [
                {
                    "id": "000004095652",
                    "database": "ZINC",
                    "url": ""
                },
                {
                    "id": "61694",
                    "database": "ChEBI",
                    "url": ""
                },
                {
                    "id": "61694",
                    "database": "ChEBI",
                    "url": ""
                },
                {
                    "id": "000004095652",
                    "database": "ZINC",
                    "url": ""
                },
                {
                    "id": "61694",
                    "database": "ChEBI",
                    "url": ""
                },
                {
                    "id": "000004095652",
                    "database": "ZINC",
                    "url": ""
                },
                {
                    "id": "61694",
                    "database": "ChEBI",
                    "url": ""
                },
                {
                    "id": "000004095652",
                    "database": "ZINC",
                    "url": ""
                },
                {
                    "id": "61694",
                    "database": "ChEBI",
                    "url": ""
                },
                {
                    "id": "000004095652",
                    "database": "ZINC",
                    "url": ""
                },
                {
                    "id": "61694",
                    "database": "ChEBI",
                    "url": ""
                },
                {
                    "id": "000004095652",
                    "database": "ZINC",
                    "url": ""
                }
            ]
        }
    ],
    "reaction_enzymes": [
        {
            "uniprot_ac": "B3STU3",
            "gene_name": "Chac1",
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