{ "uniprot_canonical_ac": "Q99M04-1", "uniprot_ac": "Q99M04", "uniprot_id": "LIAS_MOUSE", "mass": { "chemical_mass": 41879.0, "monoisotopic_mass": 41879.0 }, "refseq": { "ac": "NP_001297541", "name": "lipoyl synthase, mitochondrial isoform 2", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541" }, "gene_names": [ { "name": "Lias", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/Q99M04", "id": "Q99M04" }, { "name": "2900022L22Rik; 4933425M12Rik; 7a5ex; lip-syn; LS", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541", "id": "NP_001297541" }, { "name": "LIAS", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/Q99M04", "id": "Q99M04" }, { "name": "Lias", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541", "id": "NP_001297541" }, { "name": "Lip1", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/Q99M04", "id": "Q99M04" } ], "protein_names": [ { "name": "Lipoyl synthase, mitochondrial", "resource": "UniProtKB", "type": "recommended", "url": "", "id": "Q99M04" }, { "name": "2.8.1.8", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "Q99M04" }, { "name": "LS", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "Q99M04" }, { "name": "Lip-syn", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "Q99M04" }, { "name": "Lipoate synthase", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "Q99M04" }, { "name": "Lipoic acid synthase", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "Q99M04" }, { "name": "lipoyl synthase, mitochondrial isoform 2", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541", "id": "NP_001297541" }, { "name": "lipoyl synthase, mitochondrial; lipoate synthase; lipoic acid synthase", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541", "id": "NP_001297541" }, { "name": "mLIP1", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "Q99M04" } ], "function": [ { "annotation": "Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.", "evidence": [ { "database": "UniProtKB", "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04#function" }, { "database": "UniProtKB", "id": "A0A0M3HEP3", "url": "https://www.uniprot.org/uniprot/A0A0M3HEP3#function" }, { "database": "UniProtKB", "id": "MF_03123", "url": "https://www.uniprot.org/uniprot/MF_03123#function" } ] }, { "annotation": "Decreased endogenous lipoic acid gene expression plays a role in development of diabetic atherosclerosis in ApoE deficient mice.", "evidence": [ { "database": "RefSeq", "id": "NP_001297541", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541" }, { "database": "PubMed", "id": "22658261", "url": "https://glygen.org/publication/PubMed/22658261" } ] }, { "annotation": "Deficiency increases oxidative stress and accelerates the development of diabetic nephropathy", "evidence": [ { "database": "RefSeq", "id": "NP_001297541", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541" }, { "database": "PubMed", "id": "22021711", "url": "https://glygen.org/publication/PubMed/22021711" } ] }, { "annotation": "Development of homozygous embryos lacking Lias is retarded.", "evidence": [ { "database": "RefSeq", "id": "NP_001297541", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541" }, { "database": "PubMed", "id": "16135825", "url": "https://glygen.org/publication/PubMed/16135825" } ] }, { "annotation": "Enhanced neutrophil sequestration in the livers of Lias+/- mice was associated with increased hepatocyte injury and increased gene expression of growth-related oncogene, E-selectin, and VCAM-1 in the liver and/or lung.", "evidence": [ { "database": "RefSeq", "id": "NP_001297541", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541" }, { "database": "PubMed", "id": "18845616", "url": "https://glygen.org/publication/PubMed/18845616" } ] }, { "annotation": "Lias overexpression alleviates pulmonary injury induced by fine particulate matter in mice.", "evidence": [ { "database": "RefSeq", "id": "NP_001297541", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541" }, { "database": "PubMed", "id": "37341891", "url": "https://glygen.org/publication/PubMed/37341891" } ] }, { "annotation": "Overexpression of the Lias gene attenuates hepatic steatosis in Leprdb/db mice.", "evidence": [ { "database": "RefSeq", "id": "NP_001297541", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541" }, { "database": "PubMed", "id": "33263565", "url": "https://glygen.org/publication/PubMed/33263565" } ] } ], "disease": [ { "disease_id": "DOID:3649", "recommended_name": { "id": "DOID:3649", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:3649", "name": "pyruvate decarboxylase deficiency", "description": "A carbohydrate metabolic disorder characterized by the buildup of lactic acid in the body and a variety of neurological problems and caused by a deficiency of one of the three enzymes in the pyruvate dehydrogenase complex." }, "synonyms": [ { "id": "DOID:3649", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:3649", "name": "deficiency of pyruvic dehydrogenase", "description": "" }, { "id": "DOID:3649", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:3649", "name": "pyruvate dehydrogenase complex deficiency disease", "description": "" }, { "id": "DOID:3649", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:3649", "name": "pyruvate dehydrogenase deficiency", "description": "" }, { "id": "MONDO:0012120", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012120", "name": "pyruvate dehydrogenase phosphatase deficiency", "description": "Pyruvate dehydrogenase phosphatase deficiency is a very rare subtype of pyruvate dehydrogenase deficiency (PDHD) characterized by lactic acidemia in the neonatal period." }, { "id": "MONDO:0012120", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012120", "name": "PDH phosphatase deficiency", "description": "" }, { "id": "MONDO:0012120", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012120", "name": "pyruvate dehydrogenase phosphatase deficiency", "description": "" }, { "id": "MONDO:0012120", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012120", "name": "PDHPD", "description": "" }, { "id": "MONDO:0012120", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012120", "name": "lactic acidemia with pyruvate dehydrogenase phosphatase deficiency", "description": "" }, { "id": "MONDO:0013580", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0013580", "name": "pyruvate dehydrogenase E1-beta deficiency", "description": "Pyruvate dehydrogenase E1-beta deficiency is an extremely rare form of pyruvate dehydrogenase deficiency (PDHD) characterized by severe lactic acidosis, developmental delay and hypotonia." }, { "id": "MONDO:0013580", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0013580", "name": "PDHBD", "description": "" }, { "id": "MONDO:0013580", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0013580", "name": "pyruvate dehydrogenase E1-beta deficiency", "description": "" }, { "id": "MONDO:0013580", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0013580", "name": "pyruvate dehydrogenase complex E1 component subunit beta deficiency", "description": "" }, { "id": "MONDO:0013580", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0013580", "name": "pyruvate dehydrogenase E1-BETA deficiency", "description": "" }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "pyruvate dehydrogenase E1-alpha deficiency", "description": "Pyruvate dehydrogenase E1-alpha deficiency is the most frequent form of pyruvate dehydrogenase deficiency (PDHD) characterized by variable lactic acidosis, impaired psychomotor development, hypotonia and neurological dysfunction." }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "pyruvate decarboxylase deficiency", "description": "" }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "PDHAD", "description": "" }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "pyruvate dehydrogenase E1-alpha deficiency", "description": "" }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "pyruvate dehydrogenase complex E1 component subunit alpha deficiency", "description": "" }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "pyruvate dehydrogenase e1-alpha deficiency, X-linked dominant", "description": "" }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "PDH deficiency", "description": "" }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "ataxia with lactic acidosis 1", "description": "" }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "ataxia, intermittent, with abnormal pyruvate metabolism", "description": "" }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "ataxia, intermittent, with pyruvate dehydrogenase deficiency", "description": "" }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "ataxia, intermittent, with pyruvate dehydrogenase, or decarboxylase, deficiency", "description": "" }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "lactic acidemia, thiamine-responsive", "description": "" }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "pyruvate dehydrogenase Complex deficiency", "description": "" }, { "id": "MONDO:0010717", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010717", "name": "pyruvate dehydrogenase E1-ALPHA deficiency", "description": "" }, { "id": "MONDO:0009502", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009502", "name": "pyruvate dehydrogenase E2 deficiency", "description": "Pyruvate dehydrogenase E2 deficiency is a very rare form of pyruvate dehydrogenase deficiency (PDHD) characterized by variable lactic acidosis and neurological dysfunction, mainly appearing during childhood." }, { "id": "MONDO:0009502", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009502", "name": "dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex deficiency", "description": "" }, { "id": "MONDO:0009502", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009502", "name": "dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex deficiency", "description": "" }, { "id": "MONDO:0009502", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009502", "name": "pyruvate dehydrogenase E2 deficiency", "description": "" }, { "id": "MONDO:0009502", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009502", "name": "pyruvate dehydrogenase complex component E2 deficiency", "description": "" }, { "id": "MONDO:0009502", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009502", "name": "PDHDD", "description": "" }, { "id": "MONDO:0009502", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009502", "name": "lactic acidemia due to defect of E2 lipoyl transacetylase of the pyruvate dehydrogenase Complex", "description": "" }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "pyruvate dehydrogenase E3-binding protein deficiency", "description": "Pyruvate dehydrogenase E3-binding protein deficiency is a rare mild form of pyruvate dehydrogenase deficiency (PDHD) characterized by variable lactic acidosis and neurological dysfunction." }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "2-oxoglutarate complex deficiency", "description": "" }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "Glycine cleavage system L protein deficiency", "description": "" }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "branched chain alpha-ketoacid dehydrogenase complex deficiency", "description": "" }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "diaphorase deficiency", "description": "" }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "dihydrolipoyl dehydrogenase deficiency", "description": "" }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "lacticacidemia due to PDX1 deficiency", "description": "" }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "lipoamide dehydrogenase deficiency", "description": "" }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "pyruvate dehydrogenase E3-binding protein deficiency", "description": "" }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "pyruvate dehydrogenase complex component E3 deficiency", "description": "" }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "pyruvate dehydrogenase protein X component deficiency", "description": "" }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "PDHXD", "description": "" }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "lactic acidemia due to defect in lipoyl-containing component 10 of the pyruvate dehydrogenase Complex", "description": "" }, { "id": "MONDO:0009503", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0009503", "name": "pyruvate dehydrogenase E3-binding PROTEIN deficiency", "description": "" }, { "id": "MIM:608782", "resource": "MIM", "url": "https://www.omim.org/entry/608782", "name": "Pyruvate dehydrogenase phosphatase deficiency", "description": "Pyruvate dehydrogenase phosphatase deficiency" }, { "id": "MIM:614111", "resource": "MIM", "url": "https://www.omim.org/entry/614111", "name": "Pyruvate dehydrogenase E1-beta deficiency", "description": "Pyruvate dehydrogenase E1-beta deficiency" }, { "id": "MIM:312170", "resource": "MIM", "url": "https://www.omim.org/entry/312170", "name": "Pyruvate dehydrogenase E1-alpha deficiency", "description": "Pyruvate dehydrogenase E1-alpha deficiency" }, { "id": "MIM:245348", "resource": "MIM", "url": "https://www.omim.org/entry/245348", "name": "Pyruvate dehydrogenase E2 deficiency", "description": "Pyruvate dehydrogenase E2 deficiency" }, { "id": "MIM:245349", "resource": "MIM", "url": "https://www.omim.org/entry/245349", "name": "Pyruvate dehydrogenase E3-binding protein deficiency", "description": "Pyruvate dehydrogenase E3-binding protein deficiency" } ], "evidence": [ { "id": "3649", "database": "Alliance of Genome Resources", "url": "https://www.alliancegenome.org/disease/DOID:3649" } ] } ], "snv": [], "mutagenesis": [], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Mus musculus", "taxid": 10090, "evidence": [ { "database": "UniProtKB", "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04" } ], "common_name": "Mouse" } ], "gene": [ { "name": "Lias", "url": "https://www.uniprot.org/uniprot/Q99M04", "locus": { "chromosome": "5", "start_pos": 65568036, "end_pos": 65548840, "strand": "-", "evidence": [ { "database": "Ensembl Gene", "id": "ENSMUSG00000029199", "url": "https://www.ensembl.org/id/ENSMUSG00000029199" } ] } } ], "crossref": [ { "id": "ENSMUSG00000029199", "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000029199", "database": "Bgee", "categories": [ "Gene Expression" ] }, { "id": "cd01335", "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd01335", "database": "CDD", "categories": [ "Protein Classification" ] }, { "id": "79464", "url": "https://www.ncbi.nlm.nih.gov/gene/79464", "database": "GeneID", "categories": [ "Genome Annotation" ] }, { "id": "IPR003698", "url": "https://www.ebi.ac.uk/interpro/entry/IPR003698", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR006638", "url": "https://www.ebi.ac.uk/interpro/entry/IPR006638", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR007197", "url": "https://www.ebi.ac.uk/interpro/entry/IPR007197", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR013785", "url": "https://www.ebi.ac.uk/interpro/entry/IPR013785", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR031691", "url": "https://www.ebi.ac.uk/interpro/entry/IPR031691", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "mmu:79464", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:79464", "database": "KEGG Pathway", "categories": [ "Enzyme and Pathway" ] }, { "id": "1934604", "url": "http://www.informatics.jax.org/marker/MGI:1934604", "database": "MGI", "categories": [ "Organism-specific" ] }, { "id": "PYCDIDF", "url": "https://omabrowser.org/oma/group/PYCDIDF/", "database": "OMA", "categories": [ "Protein Classification" ] }, { "id": "575at2759", "url": "http://www.orthodb.org/?query=575at2759", "database": "OrthoDB", "categories": [ "Protein Classification" ] }, { "id": "PTHR10949", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR10949", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PTHR10949:SF0", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR10949:SF0", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PF04055", "url": "http://pfam.xfam.org/family/PF04055", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PF16881", "url": "http://pfam.xfam.org/family/PF16881", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PR_Q99M04", "url": "http://purl.obolibrary.org/obo/PR_Q99M04", "database": "PRO", "categories": [ "Protein" ] }, { "id": "R-MMU-389661", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-389661", "database": "Reactome", "categories": [ "Protein Classification" ] }, { "id": "NP_001297541", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541", "database": "RefSeq", "categories": [ "Protein" ] }, { "id": "16585", "url": "https://www.rhea-db.org/rhea/16585", "database": "Rhea", "categories": [ "Enzyme and Pathway" ] }, { "id": "A0A0G2JFF7", "url": "https://www.uniprot.org/uniprot/A0A0G2JFF7", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "A0A0G2JFF7-1", "url": "https://www.uniprot.org/uniprot/A0A0G2JFF7-1", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "A0A0M3HEP3", "url": "https://www.uniprot.org/uniprot/A0A0M3HEP3", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "A0A0M3HEP3-1", "url": "https://www.uniprot.org/uniprot/A0A0M3HEP3-1", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "Q99M04-1", "url": "https://www.uniprot.org/uniprot/Q99M04-1", "database": "UniProtKB", "categories": [ "Protein" ] } ], "sequence": { "sequence": "MALRCWDTARSLGSRIFGRYAFTVRALSSLPDKKKEFLHNGPDLQDFVSGDLADKSTWDEYKGNLKRQKGERLRLPPWLKTKIPMGKNYNKLKNTLRNLSLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDANEPDNTAKAIAEWGLDYVVLTSVDRDDVADGGAEHIAKTVSCLKERNPKILVECLTPDFRGDLRAVEKVALSGLDVYAHNVETVPELQRKVRDPRANFDQSLRVLRHAKEVQPDVVSKTSIMLGLGETDEQVYATLKALRAADVDCLTLGQYMQPTKRHLKVEEYVTPEKFKYWEKVGNELGFLYTASGPLVRSSYKAGEFFLKNLVARRKTKASKV", "length": 373, "header": "sp|Q99M04-1|LIAS_MOUSE Isoform 1 of Lipoyl synthase, mitochondrial OS=Mus musculus OX=10090 GN=Lias PE=1 SV=1 CANONICAL=Q99M04-1" }, "isoforms": [ { "isoform_ac": "A0A0G2JFF7-1", "url": "", "sequence": { "sequence": "MGKNYNKLKNTLRNLSLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDANE", "length": 74, "header": "tr|A0A0G2JFF7-1|A0A0G2JFF7_MOUSE Computationally mapped isoform of Lipoic acid synthetase OS=Mus musculus OX=10090 GN=Lias PE=1 SV=1 CANONICAL=Q99M04-1" }, "locus": { "chromosome": "5", "start_pos": 65555029, "end_pos": 65551321, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000200374.2", "url": "https://www.ensembl.org/id/ENSMUST00000200374.2" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000143152.2", "url": "https://www.ensembl.org/id/ENSMUSP00000143152.2" } ] } }, { "isoform_ac": "A0A0M3HEP3-1", "url": "", "sequence": { "sequence": "MGKNYNKLKNTLRNLSLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDANEPDNTAKAIAEWGLDYVVLTSVDRDDVADGGAEHIAKTVSCLKERNPKILVECLTPDFRGDLRAVEKVALSGLDVYAHNVETVPELQRKVRDPRANFDQSLRVLRHAKEVQPDVVSKTSIMLGLGETDEQVYATLKALRAADVDCLTLGQYMQPTKRHLKVEEYVTPEKFKYWEKVGNELGFLYTASGPLVRSSYKAGEFFLKNLVARRKTKASKV", "length": 289, "header": "tr|A0A0M3HEP3-1|A0A0M3HEP3_MOUSE Computationally mapped isoform of Lipoyl synthase, mitochondrial OS=Mus musculus OX=10090 GN=Lias PE=1 SV=1 CANONICAL=Q99M04-1" }, "locus": { "chromosome": "5", "start_pos": 65566133, "end_pos": 65551321, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000031101.10", "url": "https://www.ensembl.org/id/ENSMUST00000031101.10" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000031101.5", "url": "https://www.ensembl.org/id/ENSMUSP00000031101.5" } ] } }, { "isoform_ac": "Q99M04-1", "url": "", "sequence": { "sequence": "MALRCWDTARSLGSRIFGRYAFTVRALSSLPDKKKEFLHNGPDLQDFVSGDLADKSTWDEYKGNLKRQKGERLRLPPWLKTKIPMGKNYNKLKNTLRNLSLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDANEPDNTAKAIAEWGLDYVVLTSVDRDDVADGGAEHIAKTVSCLKERNPKILVECLTPDFRGDLRAVEKVALSGLDVYAHNVETVPELQRKVRDPRANFDQSLRVLRHAKEVQPDVVSKTSIMLGLGETDEQVYATLKALRAADVDCLTLGQYMQPTKRHLKVEEYVTPEKFKYWEKVGNELGFLYTASGPLVRSSYKAGEFFLKNLVARRKTKASKV", "length": 373, "header": "sp|Q99M04-1|LIAS_MOUSE Isoform 1 of Lipoyl synthase, mitochondrial OS=Mus musculus OX=10090 GN=Lias PE=1 SV=1 CANONICAL=Q99M04-1" }, "locus": { "chromosome": "5", "start_pos": 65566133, "end_pos": 65548972, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000122026", "url": "https://www.ensembl.org/id/ENSMUST00000122026" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000113228", "url": "https://www.ensembl.org/id/ENSMUSP00000113228" } ] } } ], "glycosylation": [], "phosphorylation": [], "glycation": [], "keywords": [ "enzyme" ], "publication": [ { "title": "Do mammalian cells synthesize lipoic acid? Identification of a mouse cDNA encoding a lipoic acid synthase located in mitochondria.", "journal": "FEBS Lett.", "date": "2001", "authors": "Morikawa T, Yasuno R, Wada H", "evidence": [ { "database": "UniProtKB", "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04" } ], "reference": [ { "type": "PubMed", "id": "11389890", "url": "https://glygen.org/publication/PubMed/11389890" } ] }, { "title": "Endogenous production of lipoic acid is essential for mouse development.", "journal": "Molecular and cellular biology", "date": "2005", "authors": "Yi X, Maeda N", "evidence": [ { "database": "RefSeq", "id": "NP_001297541", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541" } ], "reference": [ { "type": "PubMed", "id": "16135825", "url": "https://glygen.org/publication/PubMed/16135825" } ] }, { "title": "Mice with heterozygous deficiency of lipoic acid synthase have an increased sensitivity to lipopolysaccharide-induced tissue injury.", "journal": "Journal of leukocyte biology", "date": "2009", "authors": "Yi X, Kim K, Yuan W, Xu L, Kim HS, Homeister JW, Key NS, Maeda N", "evidence": [ { "database": "RefSeq", "id": "NP_001297541", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541" } ], "reference": [ { "type": "PubMed", "id": "18845616", "url": "https://glygen.org/publication/PubMed/18845616" } ] }, { "title": "Reduced expression of lipoic acid synthase accelerates diabetic nephropathy.", "journal": "Journal of the American Society of Nephrology : JASN", "date": "2012", "authors": "Yi X, Xu L, Hiller S, Kim HS, Nickeleit V, James LR, Maeda N", "evidence": [ { "database": "RefSeq", "id": "NP_001297541", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541" } ], "reference": [ { "type": "PubMed", "id": "22021711", "url": "https://glygen.org/publication/PubMed/22021711" } ] }, { "title": "Reduced alpha-lipoic acid synthase gene expression exacerbates atherosclerosis in diabetic apolipoprotein E-deficient mice.", "journal": "Atherosclerosis", "date": "2012", "authors": "Yi X, Xu L, Hiller S, Kim HS, Maeda N", "evidence": [ { "database": "RefSeq", "id": "NP_001297541", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541" } ], "reference": [ { "type": "PubMed", "id": "22658261", "url": "https://glygen.org/publication/PubMed/22658261" } ] }, { "title": "Overexpression of the Lias gene attenuates hepatic steatosis in Leprdb/db mice.", "journal": "The Journal of endocrinology", "date": "2021", "authors": "Xu G, Yan T, Peng Q, Li H, Wu W, Yi X, Zhao Y", "evidence": [ { "database": "RefSeq", "id": "NP_001297541", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541" } ], "reference": [ { "type": "PubMed", "id": "33263565", "url": "https://glygen.org/publication/PubMed/33263565" } ] }, { "title": "Lias overexpression alleviates pulmonary injury induced by fine particulate matter in mice.", "journal": "Environmental geochemistry and health", "date": "2023-08", "authors": "Xu Guangcui, Zhao Yingzheng, Tao Yingjun, Xiong Cheng, Lv Mengdi, Gao Qiyu, Zhang Fengquan, An Zhen, Wu Weidong", "evidence": [ { "database": "RefSeq", "id": "NP_001297541", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001297541" } ], "reference": [ { "type": "PubMed", "id": "37341891", "url": "https://glygen.org/publication/PubMed/37341891" } ] } ], "pathway": [ { "id": "mmu:79464", "name": "", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:79464", "resource": "KEGG Pathway" }, { "id": "R-MMU-389661", "name": "Glyoxylate metabolism and glycine degradation", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-389661", "resource": "Reactome", "description": "Glyoxylate metabolism and glycine degradation" } ], "orthologs": [ { "uniprot_canonical_ac": "O43766-1", "protein_name": "Lipoyl synthase, mitochondrial", "gene_name": "LIAS", "tax_id": 9606, "organism": "Homo sapiens", "sequence": { "sequence": "MSLRCGDAARTLGPRVFGRYFCSPVRPLSSLPDKKKELLQNGPDLQDFVSGDLADRSTWDEYKGNLKRQKGERLRLPPWLKTEIPMGKNYNKLKNTLRNLNLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDASEPYNTAKAIAEWGLDYVVLTSVDRDDMPDGGAEHIAKTVSYLKERNPKILVECLTPDFRGDLKAIEKVALSGLDVYAHNVETVPELQSKVRDPRANFDQSLRVLKHAKKVQPDVISKTSIMLGLGENDEQVYATMKALREADVDCLTLGQYMQPTRRHLKVEEYITPEKFKYWEKVGNELGFHYTASGPLVRSSYKAGEFFLKNLVAKRKTKDL", "length": 372 }, "evidence": [ { "id": "45237790", "url": "http://www.informatics.jax.org/homology/45237790", "database": "MGI" }, { "id": "Q99M04", "url": "https://omabrowser.org/oma/group/Q99M04/", "database": "OMA" } ], "common_name": "Human" }, { "uniprot_canonical_ac": "P32875-1", "protein_name": "Lipoyl synthase, mitochondrial", "gene_name": "LIP5", "tax_id": 559292, "organism": "Saccharomyces cerevisiae S288C", "sequence": { "sequence": "MYRRSVGVLFVGRNTRWISSTIRCGTSATRPIRSNALNTDSDNASVRVPVGNSTEVENATSQLTGTSGKRRKGNRKRITEFKDALNLGPSFADFVSGKASKMILDPLEKARQNTEEAKKLPRWLKVPIPKGTNYHKLKGDVKELGLSTVCEEARCPNIGECWGGKDKSKATATIMLLGDTCTRGCRFCSVKTNRTPSKPDPMEPENTAEAIKRWGLGYVVLTTVDRDDLVDGGANHLAETVRKIKQKAPNTLVETLSGDFRGDLKMVDIMAQCGLDVYAHNLETVESLTPHVRDRRATYRQSLSVLERAKATVPSLITKTSIMLGLGETDEQITQTLKDLRNIQCDVVTFGQYMRPTKRHMKVVEYVKPEKFDYWKERALEMGFLYCASGPLVRSSYKAGEAFIENVLKKRNMK", "length": 414 }, "evidence": [ { "id": "Q99M04", "url": "https://omabrowser.org/oma/group/Q99M04/", "database": "OMA" } ], "common_name": "Yeast" }, { "uniprot_canonical_ac": "Q5XIH4-1", "protein_name": "Lipoyl synthase, mitochondrial", "gene_name": "Lias", "tax_id": 10116, "organism": "Rattus norvegicus", "sequence": { "sequence": "MALRCWDAARSLGSRIFGRYACSVRALSSLPDEKKKFLHNGPDLQDFVSGDLADKSTWDDYKGNLKRQKGERLRLPPWLKTKIPMGKNYNKLKNTLRNLNLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDPSEPDNTARAIAEWGLDYVVLTSVDRDDVVDGGAEHIAKTVSCLKERNPKILVECLTPDFRGDLRAVEKVALSGLDVYAHNVETVPELQRKVRDPRANFDQSLRVLKHAKEVQPDVVSKTSIMLGLGETDEQVYATMKALRAADVDCLTLGQYMQPTKRHLKVEEYVTPEKFKYWEEVGNELGFHYTASGPLVRSSYKAGEFFLKNLVAKRKTKVSKV", "length": 373 }, "evidence": [ { "id": "45237790", "url": "http://www.informatics.jax.org/homology/45237790", "database": "MGI" }, { "id": "Q99M04", "url": "https://omabrowser.org/oma/group/Q99M04/", "database": "OMA" } ], "common_name": "Rat" }, { "uniprot_canonical_ac": "Q7JQW6-1", "protein_name": "Lipoyl synthase, mitochondrial", "gene_name": "Las", "tax_id": 7227, "organism": "Drosophila melanogaster", "sequence": { "sequence": "MLRALKTHAEAPIVVATRAASTNAEKLEEIRERLAKGPNFHDFVQNPDNTRNEWEQYDGKLRREKGEEQRLRLPPWLKTTIPVGKNYAKIKAQMRELKLSTVCEEARCPNIGECWGGGEHGTQTATIMLMGDTCTRGCRFCSVKTARKPPPLDVNEPVNTATAIASWGLDYIVLTSVDRDDLPDGGSEHIAETVREIKARNSNIFVECLVPDFRGNLECVKTIANSGLDVYAHNIETVEKLTPYVRDRRAHYRQTLQVLTEAKRFNPNLITKSSIMLGLGETDEEIENTLKDLREAGVDCVTLGQYMQPTNKHLKVIEYVTPEKFKHWEERGNELGFLYTASGPLVRSSYKAGEFFITSILENRKKRQNDTEVPKKQ", "length": 377 }, "evidence": [ { "id": "Q99M04", "url": "https://omabrowser.org/oma/group/Q99M04/", "database": "OMA" } ], "common_name": "Fruit fly" } ], "go_annotation": { "categories": [ { "name": "Molecular Function", "total": 3, "go_terms": [ { "name": "4 iron, 4 sulfur cluster binding", "id": "GO:0051539", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0051539", "evidence": [ { "database": "UniProtKB", "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04" } ], "pmid": "" }, { "name": "lipoate synthase activity", "id": "GO:0016992", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016992", "evidence": [ { "database": "UniProtKB", "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04" } ], "pmid": "" }, { "name": "metal ion binding", "id": "GO:0046872", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046872", "evidence": [ { "database": "UniProtKB", "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04" } ], "pmid": "" } ] }, { "name": "Cellular Component", "total": 1, "go_terms": [ { "name": "mitochondrion", "id": "GO:0005739", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005739", "evidence": [ { "database": "UniProtKB", "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04" } ], "pmid": "" } ] }, { "name": "Biological Process", "total": 6, "go_terms": [ { "name": "inflammatory response", "id": "GO:0006954", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006954", "evidence": [ { "database": "UniProtKB", "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04" } ], "pmid": "" }, { "name": "response to oxidative stress", "id": "GO:0006979", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006979", "evidence": [ { "database": "UniProtKB", "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04" } ], "pmid": "" }, { "name": "response to lipopolysaccharide", "id": "GO:0032496", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0032496", "evidence": [ { "database": "UniProtKB", "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04" } ], "pmid": "" }, { "name": "neural tube closure", "id": "GO:0001843", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0001843", "evidence": [ { "database": "UniProtKB", "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04" } ], "pmid": "" }, { "name": "protein lipoylation", "id": "GO:0009249", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0009249", "evidence": [ { "database": "UniProtKB", "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04" } ], "pmid": "" }, { "name": "lipoate biosynthetic process", "id": "GO:0009107", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0009107", "evidence": [ { "database": "UniProtKB", "id": "Q99M04", "url": "https://www.uniprot.org/uniprot/Q99M04" } ], "pmid": "" } ] } ] }, "pro_annotation": [], "site_annotation": [ { "start_pos": 98, "end_pos": 100, "annotation": "n_glycosylation_sequon" } ], "ptm_annotation": [], "interactions": [], "reactions": [ { "id": "16585", "name": "", "equation": "[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]", "summary": "", "cellular_location": "", "input_participant_list": [], "output_participant_list": [], "enzyme_list": [ "Q5XIH4-1", "O43766-1", "Q99M04-1", "P32875-1", "Q7JQW6-1" ], "evidence": [ { "id": "16585", "database": "Rhea", "url": "https://www.rhea-db.org/rhea/16585" } ] }, { "id": "R-MMU-6793591", "name": "LIAS:2(4Fe-4S) transforms octanoyl-K107-GCSH to lipoyl-K107-GCSH", "equation": "", "summary": "This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp", "cellular_location": "mitochondrial matrix", "input_participant_list": [ "R-ALL-6793610" ], "output_participant_list": [ "R-ALL-379705", "R-ALL-6793613" ], "enzyme_list": [ "Q99M04-1" ], "evidence": [ { "id": "R-MMU-6793591", "database": "Reactome", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-6793591" } ] } ], "reaction_participants": [ { "participant_id": "R-ALL-379705", "participant_name": "L-Met [mitochondrial matrix]", "crossref": [ { "id": "57844", "database": "ChEBI", "url": "" }, { "id": "000001532529", "database": "ZINC", "url": "" }, { "id": "57844", "database": "ChEBI", "url": "" }, { "id": "000001532529", "database": "ZINC", "url": "" }, { "id": "57844", "database": "ChEBI", "url": "" }, { "id": "000001532529", "database": "ZINC", "url": "" }, { "id": "57844", "database": "ChEBI", "url": "" }, { "id": "000001532529", "database": "ZINC", "url": "" }, { "id": "57844", "database": "ChEBI", "url": "" }, { "id": "000001532529", "database": "ZINC", "url": "" } ] }, { "participant_id": "R-ALL-6793610", "participant_name": "S [mitochondrial matrix]", "crossref": [ { "id": "26833", "database": "ChEBI", "url": "" }, { "id": "26833", "database": "ChEBI", "url": "" }, { "id": "26833", "database": "ChEBI", "url": "" }, { "id": "26833", "database": "ChEBI", "url": "" }, { "id": "26833", "database": "ChEBI", "url": "" } ] }, { "participant_id": "R-ALL-6793613", "participant_name": "5dAde [mitochondrial matrix]", "crossref": [ { "id": "17319", "database": "ChEBI", "url": "" }, { "id": "000001999286", "database": "ZINC", "url": "" }, { "id": "C05198", "database": "COMPOUND", "url": "" }, { "id": "HMDB0001983", "database": "HMDB Metabolite", "url": "" }, { "id": "17319", "database": "ChEBI", "url": "" }, { "id": "000001999286", "database": "ZINC", "url": "" }, { "id": "C05198", "database": "COMPOUND", "url": "" }, { "id": "HMDB0001983", "database": "HMDB Metabolite", "url": "" }, { "id": "17319", "database": "ChEBI", "url": "" }, { "id": "000001999286", "database": "ZINC", "url": "" }, { "id": "C05198", "database": "COMPOUND", "url": "" }, { "id": "HMDB0001983", "database": "HMDB Metabolite", "url": "" }, { "id": "C05198", "database": "COMPOUND", "url": "" }, { "id": "17319", "database": "ChEBI", "url": "" }, { "id": "HMDB0001983", "database": "HMDB Metabolite", "url": "" }, { "id": "000001999286", "database": "ZINC", "url": "" }, { "id": "17319", "database": "ChEBI", "url": "" }, { "id": "000001999286", "database": "ZINC", "url": "" }, { "id": "C05198", "database": "COMPOUND", "url": "" }, { "id": "HMDB0001983", "database": "HMDB Metabolite", "url": "" } ] } ], "reaction_enzymes": [ { "uniprot_ac": "O43766", "gene_name": "LIAS", "activity": [ { "ec_number": "2.8.1.8", "activity": "[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin].", "evidence": [ { "id": "O43766", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/O43766" } ] }, { "ec_number": "2.8.1.8", "activity": "", "evidence": [ { "id": "O43766", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/O43766" } ] } ] }, { "uniprot_ac": "P32875", "gene_name": "LIP5", "activity": [ { "ec_number": "2.8.1.8", "activity": "[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin].", "evidence": [ { "id": "P32875", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P32875" } ] }, { "ec_number": "2.8.1.8", "activity": "", "evidence": [ { "id": "P32875", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P32875" } ] } ] }, { "uniprot_ac": "Q5XIH4", "gene_name": "Lias", "activity": [ { "ec_number": "2.8.1.8", "activity": "[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin].", "evidence": [ { "id": "Q5XIH4", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q5XIH4" } ] }, { "ec_number": "2.8.1.8", "activity": "", "evidence": [ { "id": "Q5XIH4", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q5XIH4" } ] } ] }, { "uniprot_ac": "Q7JQW6", "gene_name": "Las", "activity": 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