{
    "uniprot_canonical_ac": "Q3TBV4-1",
    "uniprot_ac": "Q3TBV4",
    "uniprot_id": "Q3TBV4_MOUSE",
    "mass": {
        "chemical_mass": 33807.0,
        "monoisotopic_mass": 33807.0
    },
    "refseq": {
        "ac": "NP_001028697",
        "name": "potassium channel subfamily K member 6",
        "summary": "",
        "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697"
    },
    "gene_names": [
        {
            "name": "Kcnk6",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "https://www.uniprot.org/uniprot/Q3TBV4",
            "id": "Q3TBV4"
        },
        {
            "name": "D7Ertd764e; Toss; Twik2",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697",
            "id": "NP_001028697"
        },
        {
            "name": "Kcnk6",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697",
            "id": "NP_001028697"
        }
    ],
    "protein_names": [
        {
            "name": "Potassium channel subfamily K member",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "",
            "id": "Q3TBV4"
        },
        {
            "name": "TWIK-2 two-pore-domain K+ channel",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697",
            "id": "NP_001028697"
        },
        {
            "name": "potassium channel subfamily K member 6",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697",
            "id": "NP_001028697"
        }
    ],
    "function": [
        {
            "annotation": "Deletion of Kcnk6 (encoding two-pore domain K(+) channel TWIK2) prevents NLR Family, Pyrin Domain-Containing 3 Protein (NLRP3) inflammasome activation in macrophages and suppressed sepsis-induced lung inflammation. Adoptive transfer of Kcnk6(-/-) macrophages into mouse airways after macrophage depletion also prevents inflammatory lung injury.",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001028697",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697"
                },
                {
                    "database": "PubMed",
                    "id": "29958799",
                    "url": "https://glygen.org/publication/PubMed/29958799"
                }
            ]
        },
        {
            "annotation": "Endosomal trafficking of two-pore K[+] efflux channel TWIK2 to plasmalemma mediates NLRP3 inflammasome activation and inflammatory injury. Publication Status: Online-Only",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001028697",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697"
                },
                {
                    "database": "PubMed",
                    "id": "37158595",
                    "url": "https://glygen.org/publication/PubMed/37158595"
                }
            ]
        },
        {
            "annotation": "Mice deficient in the TWIK-2 channel develop pulmonary hypertension between 8 and 20 weeks of age through a mechanism involving Rho-kinase.",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001028697",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697"
                },
                {
                    "database": "PubMed",
                    "id": "25245387",
                    "url": "https://glygen.org/publication/PubMed/25245387"
                }
            ]
        },
        {
            "annotation": "Twik-2-/- mice have enhanced vasocontractility in the distal pulmonary vasculature.",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001028697",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697"
                },
                {
                    "database": "PubMed",
                    "id": "30632293",
                    "url": "https://glygen.org/publication/PubMed/30632293"
                }
            ]
        }
    ],
    "disease": [
        {
            "disease_id": "DOID:3454",
            "recommended_name": {
                "id": "DOID:3454",
                "resource": "Disease Ontology",
                "url": "http://disease-ontology.org/term/DOID:3454",
                "name": "brain infarction",
                "description": "A cerebrovascular disease that is characterized by tissue necrosis located_in the brain, resulting from inadequate blood flow through the brain."
            },
            "synonyms": [],
            "evidence": [
                {
                    "id": "3454",
                    "database": "Alliance of Genome Resources",
                    "url": "https://www.alliancegenome.org/disease/DOID:3454"
                }
            ]
        }
    ],
    "snv": [],
    "mutagenesis": [],
    "expression_tissue": [],
    "expression_disease": [],
    "species": [
        {
            "name": "Mus musculus",
            "taxid": 10090,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q3TBV4",
                    "url": "https://www.uniprot.org/uniprot/Q3TBV4"
                }
            ],
            "common_name": "Mouse"
        }
    ],
    "gene": [
        {
            "name": "Kcnk6",
            "url": "https://www.uniprot.org/uniprot/Q3TBV4",
            "locus": {
                "chromosome": "7",
                "start_pos": 28921351,
                "end_pos": 28931940,
                "strand": "+",
                "evidence": [
                    {
                        "database": "Ensembl Gene",
                        "id": "ENSMUSG00000046410",
                        "url": "https://www.ensembl.org/id/ENSMUSG00000046410"
                    }
                ]
            }
        }
    ],
    "crossref": [
        {
            "id": "ENSMUSG00000046410",
            "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000046410",
            "database": "Bgee",
            "categories": [
                "Gene Expression"
            ]
        },
        {
            "id": "52150",
            "url": "https://www.ncbi.nlm.nih.gov/gene/52150",
            "database": "GeneID",
            "categories": [
                "Genome Annotation"
            ]
        },
        {
            "id": "IPR003092",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR003092",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR003280",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR003280",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR005408",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR005408",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR005409",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR005409",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR013099",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR013099",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "mmu:52150",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:52150",
            "database": "KEGG Pathway",
            "categories": [
                "Enzyme and Pathway"
            ]
        },
        {
            "id": "1891291",
            "url": "http://www.informatics.jax.org/marker/MGI:1891291",
            "database": "MGI",
            "categories": [
                "Organism-specific"
            ]
        },
        {
            "id": "IERPMED",
            "url": "https://omabrowser.org/oma/group/IERPMED/",
            "database": "OMA",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "600333at2759",
            "url": "http://www.orthodb.org/?query=600333at2759",
            "database": "OrthoDB",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR11003",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR11003",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR11003:SF28",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR11003:SF28",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PF07885",
            "url": "http://pfam.xfam.org/family/PF07885",
            "database": "Pfam",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "R-MMU-1299308",
            "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-1299308",
            "database": "Reactome",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "R-MMU-5576886",
            "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-5576886",
            "database": "Reactome",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "NP_001028697",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697",
            "database": "RefSeq",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "Q3TBV4",
            "url": "https://www.uniprot.org/uniprot/Q3TBV4",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "Q3TBV4-1",
            "url": "https://www.uniprot.org/uniprot/Q3TBV4-1",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        }
    ],
    "sequence": {
        "sequence": "MRRGALLASALAAYAGYLALGALLVARLERPHEARLRAELGTLREQLLQHSPCVAAHALDAFVERVLAAGRLGRAALANASGAANASDPAWDFASALFFASTLVTTVGYGYTTPLTDAGKAFSIVFALLGVPITMLLLTASAQRLSLLLTHAPLSWLSLHWGWPPQRAARWHLVALLMVIVAIFFLVPAAVFAYLEEAWSFLDAFYFCFISLSTIGLGDYVPGEAPGQPYRALYKVLVTAYLFLGLVAMVLVLQTFRRVSDLHGLTELILLPDPDPASLSQDEDDQVAVLDARTDLHQHLSAASHADYASIPR",
        "length": 313,
        "header": "tr|Q3TBV4-1|Q3TBV4_MOUSE Isoform 1 of Potassium channel subfamily K member  OS=Mus musculus OX=10090 GN=Kcnk6 PE=1 SV=1 CANONICAL=Q3TBV4-1"
    },
    "isoforms": [
        {
            "isoform_ac": "Q3TBV4-1",
            "url": "",
            "sequence": {
                "sequence": "MRRGALLASALAAYAGYLALGALLVARLERPHEARLRAELGTLREQLLQHSPCVAAHALDAFVERVLAAGRLGRAALANASGAANASDPAWDFASALFFASTLVTTVGYGYTTPLTDAGKAFSIVFALLGVPITMLLLTASAQRLSLLLTHAPLSWLSLHWGWPPQRAARWHLVALLMVIVAIFFLVPAAVFAYLEEAWSFLDAFYFCFISLSTIGLGDYVPGEAPGQPYRALYKVLVTAYLFLGLVAMVLVLQTFRRVSDLHGLTELILLPDPDPASLSQDEDDQVAVLDARTDLHQHLSAASHADYASIPR",
                "length": 313,
                "header": "tr|Q3TBV4-1|Q3TBV4_MOUSE Isoform 1 of Potassium channel subfamily K member  OS=Mus musculus OX=10090 GN=Kcnk6 PE=1 SV=1 CANONICAL=Q3TBV4-1"
            },
            "locus": {
                "chromosome": "7",
                "start_pos": 28931908,
                "end_pos": 28924464,
                "strand": "+",
                "evidence": [
                    {
                        "database": "Ensembl Transcript",
                        "id": "ENSMUST00000085818.6",
                        "url": "https://www.ensembl.org/id/ENSMUST00000085818.6"
                    },
                    {
                        "database": "Ensembl Peptide",
                        "id": "ENSMUSP00000082975.5",
                        "url": "https://www.ensembl.org/id/ENSMUSP00000082975.5"
                    }
                ]
            }
        }
    ],
    "glycosylation": [
        {
            "glytoucan_ac": "",
            "type": "N-linked",
            "subtype": "",
            "site_category": "reported",
            "site_seq": "N",
            "relation": "attached",
            "comment": "N-linked (GlcNAc...) asparagine",
            "start_pos": 79,
            "start_aa": "Asn",
            "end_pos": 79,
            "end_aa": "Asn",
            "residue": "Asn",
            "site_lbl": "Asn79",
            "evidence": [
                {
                    "id": "Q3TBV4",
                    "database": "UniProtKB",
                    "url": "https://www.uniprot.org/uniprot/Q3TBV4#ptm_processing"
                },
                {
                    "id": "PIRSR038061-1",
                    "database": "PIR Site Rule",
                    "url": "https://proteininformationresource.org/cgi-bin/pirrule?id=PIRSR038061-1"
                }
            ]
        }
    ],
    "phosphorylation": [],
    "glycation": [],
    "keywords": [
        "glycoprotein",
        "glycoprotein_reported"
    ],
    "publication": [
        {
            "title": "Zinc and mercuric ions distinguish TRESK from the other two-pore-domain K+ channels.",
            "journal": "Mol. Pharmacol.",
            "date": "2006",
            "authors": "Czirjak G, Enyedi P",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q3TBV4",
                    "url": "https://www.uniprot.org/uniprot/Q3TBV4"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "16354767",
                    "url": "https://glygen.org/publication/PubMed/16354767"
                }
            ]
        },
        {
            "title": "The phagosomal proteome in interferon-gamma-activated macrophages.",
            "journal": "Immunity",
            "date": "2009",
            "authors": "Trost M, English L, Lemieux S, Courcelles M, Desjardins M, Thibault P",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "Q3TBV4",
                    "url": "https://www.uniprot.org/uniprot/Q3TBV4"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "19144319",
                    "url": "https://glygen.org/publication/PubMed/19144319"
                }
            ]
        },
        {
            "title": "TWIK-2 channel deficiency leads to pulmonary hypertension through a rho-kinase-mediated process.",
            "journal": "Hypertension (Dallas, Tex. : 1979)",
            "date": "2014",
            "authors": "Pandit LM, Lloyd EE, Reynolds JO, Lawrence WS, Reynolds C, Wehrens XH, Bryan RM",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001028697",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "25245387",
                    "url": "https://glygen.org/publication/PubMed/25245387"
                }
            ]
        },
        {
            "title": "The TWIK2 Potassium Efflux Channel in Macrophages Mediates NLRP3 Inflammasome-Induced Inflammation.",
            "journal": "Immunity",
            "date": "2018",
            "authors": "Di A, Xiong S, Ye Z, Malireddi RKS, Kometani S, Zhong M, Mittal M, Hong Z, Kanneganti TD, Rehman J, Malik AB",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001028697",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "29958799",
                    "url": "https://glygen.org/publication/PubMed/29958799"
                }
            ]
        },
        {
            "title": "Twik-2(-/-) mouse demonstrates pulmonary vascular heterogeneity in intracellular pathways for vasocontractility.",
            "journal": "Physiological reports",
            "date": "2019",
            "authors": "Kitagawa MG, Reynolds JO, Durgan D, Rodney G, Karmouty-Quintana H, Bryan R, Pandit LM",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001028697",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "30632293",
                    "url": "https://glygen.org/publication/PubMed/30632293"
                }
            ]
        },
        {
            "title": "Endosomal trafficking of two pore K+ efflux channel TWIK2 to plasmalemma mediates NLRP3 inflammasome activation and inflammatory injury.",
            "journal": "eLife",
            "date": "2023-05-09",
            "authors": "Huang Long Shuang, Anas Mohammad, Xu Jingsong, Zhou Bisheng, Toth Peter T, Krishnan Yamuna, Di Anke, Malik Asrar B",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001028697",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028697"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "37158595",
                    "url": "https://glygen.org/publication/PubMed/37158595"
                }
            ]
        }
    ],
    "pathway": [
        {
            "id": "mmu:52150",
            "name": "",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:52150",
            "resource": "KEGG Pathway"
        },
        {
            "id": "R-MMU-1299308",
            "name": "Tandem of pore domain in a weak inwardly rectifying K+ channels (TWIK)",
            "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-1299308",
            "resource": "Reactome",
            "description": "Tandem of pore domain in a weak inwardly rectifying K+ channels (TWIK)"
        },
        {
            "id": "R-MMU-5576886",
            "name": "Phase 4 - resting membrane potential",
            "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-5576886",
            "resource": "Reactome",
            "description": "Phase 4 - resting membrane potential"
        }
    ],
    "orthologs": [
        {
            "uniprot_canonical_ac": "G3V8R8-1",
            "protein_name": "Potassium channel subfamily K member",
            "gene_name": "Kcnk6",
            "tax_id": 10116,
            "organism": "Rattus norvegicus",
            "sequence": {
                "sequence": "MRRGALLASALVAYAGYLALGALLVARLERPHEARLRAELGTLREQLLRHSPCVAAHALDAFVERVLAAGRLGRAVLANASGPANASDPAWDFASALFFASTLVTTVGYGYTTPLTDAGKAFSIVFALLGVPITMLLLTASAQRLSLLLTHAPLSWLSLRWGWHPQRAARWHLVALLMVIVAIFFLIPAAVFAYLEEAWSFLDAFYFCFISLSTIGLGDYVPGEAPGQPYRSLYKVLVTAYLFLGLVAMVLVLQTFRRVSDLHGLTELILLPDPDPARLHQDEDDQVDILDARTDLHQHLSAASHANYASIPR",
                "length": 313
            },
            "evidence": [
                {
                    "id": "45235275",
                    "url": "http://www.informatics.jax.org/homology/45235275",
                    "database": "MGI"
                }
            ],
            "common_name": "Rat"
        },
        {
            "uniprot_canonical_ac": "Q9Y257-1",
            "protein_name": "Potassium channel subfamily K member 6",
            "gene_name": "KCNK6",
            "tax_id": 9606,
            "organism": "Homo sapiens",
            "sequence": {
                "sequence": "MRRGALLAGALAAYAAYLVLGALLVARLEGPHEARLRAELETLRAQLLQRSPCVAAPALDAFVERVLAAGRLGRVVLANASGSANASDPAWDFASALFFASTLITTVGYGYTTPLTDAGKAFSIAFALLGVPTTMLLLTASAQRLSLLLTHVPLSWLSMRWGWDPRRAACWHLVALLGVVVTVCFLVPAVIFAHLEEAWSFLDAFYFCFISLSTIGLGDYVPGEAPGQPYRALYKVLVTVYLFLGLVAMVLVLQTFRHVSDLHGLTELILLPPPCPASFNADEDDRVDILGPQPESHQQLSASSHTDYASIPR",
                "length": 313
            },
            "evidence": [
                {
                    "id": "45235275",
                    "url": "http://www.informatics.jax.org/homology/45235275",
                    "database": "MGI"
                }
            ],
            "common_name": "Human"
        }
    ],
    "go_annotation": {
        "categories": [
            {
                "name": "Molecular Function",
                "total": 3,
                "go_terms": [
                    {
                        "name": "outward rectifier potassium channel activity",
                        "id": "GO:0015271",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0015271",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "Q3TBV4",
                                "url": "https://www.uniprot.org/uniprot/Q3TBV4"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "potassium ion leak channel activity",
                        "id": "GO:0022841",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0022841",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "Q3TBV4",
                                "url": "https://www.uniprot.org/uniprot/Q3TBV4"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "potassium channel activity",
                        "id": "GO:0005267",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005267",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "Q3TBV4",
                                "url": "https://www.uniprot.org/uniprot/Q3TBV4"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            },
            {
                "name": "Biological Process",
                "total": 5,
                "go_terms": [
                    {
                        "name": "stabilization of membrane potential",
                        "id": "GO:0030322",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030322",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "Q3TBV4",
                                "url": "https://www.uniprot.org/uniprot/Q3TBV4"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "negative regulation of systemic arterial blood pressure",
                        "id": "GO:0003085",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003085",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "Q3TBV4",
                                "url": "https://www.uniprot.org/uniprot/Q3TBV4"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "regulation of systemic arterial blood pressure",
                        "id": "GO:0003073",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003073",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "Q3TBV4",
                                "url": "https://www.uniprot.org/uniprot/Q3TBV4"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "regulation of resting membrane potential",
                        "id": "GO:0060075",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0060075",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "Q3TBV4",
                                "url": "https://www.uniprot.org/uniprot/Q3TBV4"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "potassium ion transmembrane transport",
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