{ "uniprot_canonical_ac": "Q08484-1", "uniprot_ac": "Q08484", "uniprot_id": "GYP1_YEAST", "mass": { "chemical_mass": 73289.0, "monoisotopic_mass": 73289.0 }, "refseq": { "ac": "NP_014713", "name": "GTPase-activating protein GYP1", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_014713" }, "gene_names": [ { "name": "GYP1", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/Q08484", "id": "Q08484" }, { "name": "GYP1", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_014713", "id": "NP_014713" }, { "name": "YOR29-21", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_014713", "id": "NP_014713" }, { "name": "YOR29-21", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/Q08484", "id": "Q08484" } ], "protein_names": [ { "name": "GTPase-activating protein GYP1", "resource": "UniProtKB", "type": "recommended", "url": "", "id": "Q08484" }, { "name": "GAP for YPT1", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "Q08484" }, { "name": "GTPase-activating protein GYP1", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_014713", "id": "NP_014713" } ], "function": [ { "annotation": "GTPase-activating protein (GAP) that stimulates specifically the intrinsic GTPase activity of Ypt/Rab-type GTPases YPT1 and YPT7 (PubMed:11359917, PubMed:19386605). Functions on the Golgi as a negative regulator of YPT1 (PubMed:11359917). Functions on the vacuole as a negative regulator of YPT7 (PubMed:11118206, PubMed:19386605). It is also active on SEC4 and YPT51 (PubMed:10508155). Provides a catalytic arginine (arginine finger) and glutamine (glutamine finger) in trans to accelerate the GTP hydrolysis rate of the substrate GTPase (Probable) (PubMed:16855591).", "evidence": [ { "database": "UniProtKB", "id": "Q08484", "url": "https://www.uniprot.org/uniprot/Q08484#function" }, { "database": "PubMed", "id": "19386605", "url": "https://glygen.org/publication/PubMed/19386605" }, { "database": "PubMed", "id": "11359917", "url": "https://glygen.org/publication/PubMed/11359917" }, { "database": "PubMed", "id": "11118206", "url": "https://glygen.org/publication/PubMed/11118206" }, { "database": "PubMed", "id": "16855591", "url": "https://glygen.org/publication/PubMed/16855591" }, { "database": "PubMed", "id": "10508155", "url": "https://glygen.org/publication/PubMed/10508155" } ] } ], "disease": [], "snv": [], "mutagenesis": [ { "start_pos": 343, "end_pos": 343, "sequence_org": "R", "sequence_mut": "A", "comment": "Completely abolishes catalytic activity.", "evidence": [ { "database": "UniProtKB", "id": "Q08484", "url": "https://www.uniprot.org/uniprot/Q08484" }, { "database": "PubMed", "id": "16855591", "url": "https://glygen.org/publication/PubMed/16855591" }, { "database": "PubMed", "id": "10508155", "url": "https://glygen.org/publication/PubMed/10508155" } ] }, { "start_pos": 343, "end_pos": 343, "sequence_org": "R", "sequence_mut": "K", "comment": "Completely abolishes catalytic activity.", "evidence": [ { "database": "UniProtKB", "id": "Q08484", "url": "https://www.uniprot.org/uniprot/Q08484" }, { "database": "PubMed", "id": "16855591", "url": "https://glygen.org/publication/PubMed/16855591" }, { "database": "PubMed", "id": "10508155", "url": "https://glygen.org/publication/PubMed/10508155" } ] }, { "start_pos": 378, "end_pos": 378, "sequence_org": "Q", "sequence_mut": "A", "comment": "Completely abolishes catalytic activity.", "evidence": [ { "database": "UniProtKB", "id": "Q08484", "url": "https://www.uniprot.org/uniprot/Q08484" }, { "database": "PubMed", "id": "16855591", "url": "https://glygen.org/publication/PubMed/16855591" } ] } ], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Saccharomyces cerevisiae S288C", "taxid": 559292, "evidence": [ { "database": "UniProtKB", "id": "Q08484", "url": "https://www.uniprot.org/uniprot/Q08484" } ], "common_name": "Yeast" } ], "gene": [ { "name": "GYP1", "url": "https://www.uniprot.org/uniprot/Q08484", "locus": { "chromosome": "XV", "start_pos": 455907, "end_pos": 457820, "strand": "+", "evidence": [ { "database": "Ensembl Gene", "id": "YOR070C", "url": "https://www.ensembl.org/id/YOR070C" } ] } } ], "crossref": [ { "id": "854236", "url": "https://www.ncbi.nlm.nih.gov/gene/854236", "database": "GeneID", "categories": [ "Genome Annotation" ] }, { "id": "Q08484", "url": "https://www.ebi.ac.uk/intact/interactors/id:Q08484*", "database": "IntAct", "categories": [ "Other" ] }, { "id": "IPR000195", "url": "https://www.ebi.ac.uk/interpro/entry/IPR000195", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR035969", "url": "https://www.ebi.ac.uk/interpro/entry/IPR035969", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "sce:YOR070C", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+sce:YOR070C", "database": "KEGG Pathway", "categories": [ "Enzyme and Pathway" ] }, { "id": "Q08484", "url": "https://oglcnac.org/atlas/detail/Q08484", "database": "O-GlcNAc Atlas", "categories": [ "Glycoproteomics" ] }, { "id": "Q08484", "url": "https://www.oglcnac.mcw.edu/search/?query_protein=Q08484", "database": "O-GlcNAc Database", "categories": [ "Glycoproteomics" ] }, { "id": "FHVFVCA", "url": "https://omabrowser.org/oma/group/FHVFVCA/", "database": "OMA", "categories": [ "Protein Classification" ] }, { "id": "22307at2759", "url": "http://www.orthodb.org/?query=22307at2759", "database": "OrthoDB", "categories": [ "Protein Classification" ] }, { "id": "PTHR22957", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR22957", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PTHR22957:SF26", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR22957:SF26", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "1FKM", "url": "https://www.rcsb.org/structure/1FKM", "database": "PDB", "categories": [ "3D Structure" ] }, { "id": "2G77", "url": "https://www.rcsb.org/structure/2G77", "database": "PDB", "categories": [ "3D Structure" ] }, { "id": "PF00566", "url": "http://pfam.xfam.org/family/PF00566", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PR_Q08484", "url": "http://purl.obolibrary.org/obo/PR_Q08484", "database": "PRO", "categories": [ "Protein" ] }, { "id": "NP_014713", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_014713", "database": "RefSeq", "categories": [ "Protein" ] }, { "id": "S000005596", "url": "https://www.yeastgenome.org/locus/S000005596", "database": "SGD", "categories": [ "Organism-specific" ] }, { "id": "Q08484", "url": "https://www.uniprot.org/uniprot/Q08484", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "Q08484-1", "url": "https://www.uniprot.org/uniprot/Q08484-1", "database": "UniProtKB", "categories": [ "Protein" ] } ], "sequence": { "sequence": "MGVRSAAKEMHERDHNSDSSSLVTSLMKSWRISSASSSKKPSLYKMNTTESTSLPSGYASSADRDRRTSDGNFEAMAKQQASTRRTSNSYSPLRYVNPTLSTASNESPRPALLLRQHHQRHHHHQQPRHSSSGSVGNNCSNSTEPNKKGDRYFKDLDEDWSAVIDDYNMPIPILTNGGFGTPVAPTRTLSRKSTSSSINSISNMGTSAVRNSSSSFTYPQLPQLQKEKTNDSKKTQLEIENERDVQELNSIIQRISKFDNILKDKTIINQQDLRQISWNGIPKIHRPVVWKLLIGYLPVNTKRQEGFLQRKRKEYRDSLKHTFSDQHSRDIPTWHQIEIDIPRTNPHIPLYQFKSVQNSLQRILYLWAIRHPASGYVQGINDLVTPFFETFLTEYLPPSQIDDVEIKDPSTYMVDEQITDLEADTFWCLTKLLEQITDNYIHGQPGILRQVKNLSQLVKRIDADLYNHFQNEHVEFIQFAFRWMNCLLMREFQMGTVIRMWDTYLSETSQEVTSSYSMSSNDIKPPVTPTEPRVASFVTPTKDFQSPTTALSNMTPNNAVEDSGKMRQSSLNEFHVFVCAAFLIKWSDQLMEMDFQETITFLQNPPTKDWTETDIEMLLSEAFIWQSLYKDATSHWL", "length": 637, "header": "sp|Q08484-1|GYP1_YEAST Isoform 1 of GTPase-activating protein GYP1 OS=Saccharomyces cerevisiae S288C OX=559292 GN=GYP1 PE=1 SV=1 CANONICAL=Q08484-1" }, "isoforms": [ { "isoform_ac": "Q08484-1", "url": "", "sequence": { "sequence": "MGVRSAAKEMHERDHNSDSSSLVTSLMKSWRISSASSSKKPSLYKMNTTESTSLPSGYASSADRDRRTSDGNFEAMAKQQASTRRTSNSYSPLRYVNPTLSTASNESPRPALLLRQHHQRHHHHQQPRHSSSGSVGNNCSNSTEPNKKGDRYFKDLDEDWSAVIDDYNMPIPILTNGGFGTPVAPTRTLSRKSTSSSINSISNMGTSAVRNSSSSFTYPQLPQLQKEKTNDSKKTQLEIENERDVQELNSIIQRISKFDNILKDKTIINQQDLRQISWNGIPKIHRPVVWKLLIGYLPVNTKRQEGFLQRKRKEYRDSLKHTFSDQHSRDIPTWHQIEIDIPRTNPHIPLYQFKSVQNSLQRILYLWAIRHPASGYVQGINDLVTPFFETFLTEYLPPSQIDDVEIKDPSTYMVDEQITDLEADTFWCLTKLLEQITDNYIHGQPGILRQVKNLSQLVKRIDADLYNHFQNEHVEFIQFAFRWMNCLLMREFQMGTVIRMWDTYLSETSQEVTSSYSMSSNDIKPPVTPTEPRVASFVTPTKDFQSPTTALSNMTPNNAVEDSGKMRQSSLNEFHVFVCAAFLIKWSDQLMEMDFQETITFLQNPPTKDWTETDIEMLLSEAFIWQSLYKDATSHWL", "length": 637, "header": "sp|Q08484-1|GYP1_YEAST Isoform 1 of GTPase-activating protein GYP1 OS=Saccharomyces cerevisiae S288C OX=559292 GN=GYP1 PE=1 SV=1 CANONICAL=Q08484-1" }, "locus": { "chromosome": "XV", "start_pos": 457820, "end_pos": 455910, "strand": "+", "evidence": [ { "database": "Ensembl Transcript", "id": "YOR070C_mRNA", "url": "https://www.ensembl.org/id/YOR070C_mRNA" }, { "database": "Ensembl Peptide", "id": "YOR070C", "url": "https://www.ensembl.org/id/YOR070C" } ] } } ], "glycosylation": [ { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 101, "start_aa": "Ser", "end_pos": 101, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser101", "evidence": [ { "id": "Q08484", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/Q08484" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 104, "start_aa": "Ser", "end_pos": 104, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser104", "evidence": [ { "id": "Q08484", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/Q08484" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 107, "start_aa": "Ser", "end_pos": 107, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser107", "evidence": [ { "id": "Q08484", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/Q08484" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "", "relation": "attached", "comment": "O-GlcNAcylation", "evidence": [ { "id": "Q08484", "database": "O-GlcNAc Database", "url": "https://www.oglcnac.mcw.edu/search/?query_protein=Q08484" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] } ], "phosphorylation": [ { "start_pos": 104, "end_pos": 104, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "SGD", "id": "Q08484", "url": "https://www.yeastgenome.org/locus/Q08484" }, { "database": "iPTMnet", "id": "Q08484", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q08484" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ], "site_lbl": "Ser104" }, { "start_pos": 107, "end_pos": 107, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "SGD", "id": "Q08484", "url": "https://www.yeastgenome.org/locus/Q08484" }, { "database": "iPTMnet", "id": "Q08484", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q08484" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ], "site_lbl": "Ser107" }, { "start_pos": 132, "end_pos": 132, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "SGD", "id": "Q08484", "url": "https://www.yeastgenome.org/locus/Q08484" }, { "database": "iPTMnet", "id": "Q08484", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q08484" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ], "site_lbl": "Ser132" }, { "start_pos": 134, "end_pos": 134, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "SGD", "id": "Q08484", "url": "https://www.yeastgenome.org/locus/Q08484" }, { "database": "iPTMnet", "id": "Q08484", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q08484" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ], "site_lbl": "Ser134" }, { "start_pos": 193, "end_pos": 193, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "iPTMnet", "id": "Q08484", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q08484" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" }, { "database": "SGD", "id": "Q08484", "url": "https://www.yeastgenome.org/locus/Q08484" } ], "site_lbl": "Ser193" }, { "start_pos": 194, "end_pos": 194, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Thr", "comment": "", "evidence": [ { "database": "SGD", "id": "Q08484", "url": "https://www.yeastgenome.org/locus/Q08484" }, { "database": "iPTMnet", "id": "Q08484", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q08484" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" } ], "site_lbl": "Thr194" }, { "start_pos": 197, "end_pos": 197, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "iPTMnet", "id": "Q08484", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q08484" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" }, { "database": "SGD", "id": "Q08484", "url": "https://www.yeastgenome.org/locus/Q08484" } ], "site_lbl": "Ser197" }, { "start_pos": 212, "end_pos": 212, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "iPTMnet", "id": "Q08484", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q08484" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "PubMed", "id": "20702584", "url": "https://glygen.org/publication/PubMed/20702584" }, { "database": "SGD", "id": "Q08484", "url": "https://www.yeastgenome.org/locus/Q08484" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ], "site_lbl": "Ser212" }, { "start_pos": 213, "end_pos": 213, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "iPTMnet", "id": "Q08484", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q08484" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "PubMed", "id": "20702584", "url": "https://glygen.org/publication/PubMed/20702584" }, { "database": "SGD", "id": "Q08484", "url": "https://www.yeastgenome.org/locus/Q08484" } ], "site_lbl": "Ser213" }, { "start_pos": 215, "end_pos": 215, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "iPTMnet", "id": "Q08484", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q08484" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "SGD", "id": "Q08484", "url": "https://www.yeastgenome.org/locus/Q08484" } ], "site_lbl": "Ser215" }, { "start_pos": 218, "end_pos": 218, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Tyr", "comment": "", "evidence": [ { "database": "iPTMnet", "id": "Q08484", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q08484" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "SGD", "id": "Q08484", "url": "https://www.yeastgenome.org/locus/Q08484" } ], "site_lbl": "Tyr218" }, { "start_pos": 250, "end_pos": 250, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "Phosphoserine", "evidence": [ { "database": "UniProtKB", "id": "Q08484", "url": "https://www.uniprot.org/uniprot/Q08484" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "iPTMnet", "id": "Q08484", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/Q08484" }, { "database": "SGD", "id": "Q08484", "url": "https://www.yeastgenome.org/locus/Q08484" } ], "site_lbl": "Ser250" }, { "start_pos": 536, "end_pos": 536, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "SGD", "id": "Q08484", "url": 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