{ "uniprot_canonical_ac": "Q03020-1", "uniprot_ac": "Q03020", "uniprot_id": "ISU1_YEAST", "mass": { "chemical_mass": 17895.0, "monoisotopic_mass": 17895.0 }, "refseq": { "ac": "NP_015190", "name": "iron-binding protein ISU1", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_015190" }, "gene_names": [ { "name": "ISU1", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/Q03020", "id": "Q03020" }, { "name": "ISU1", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_015190", "id": "NP_015190" }, { "name": "NUA1", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_015190", "id": "NP_015190" }, { "name": "NUA1", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/Q03020", "id": "Q03020" } ], "protein_names": [ { "name": "Iron sulfur cluster assembly protein 1, mitochondrial", "resource": "UniProtKB", "type": "recommended", "url": "", "id": "Q03020" }, { "name": "Iron sulfur cluster scaffold protein 1", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "Q03020" }, { "name": "iron-binding protein ISU1", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_015190", "id": "NP_015190" } ], "function": [ { "annotation": "Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold proteins ISU1 and ISU2. In a second step, the cluster is released from ISU1/ISU2, transferred to glutaredoxin GRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISU1/ISU2 depends on the function of the cysteine desulfurase complex NFS1-ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin (YFH1) as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase ARH1 and ferredoxin YAH1, which receive their electrons from NADH. Fe-S cluster release from ISU1/ISU2 is achieved by interaction with the Hsp70 chaperone SSQ1, assisted by the DnaJ-like co-chaperone JAC1 and the nucleotide exchange factor MGE1. ISU1 is the major isoform in yeast, while ISU2 is not detectable in cells grown to stationary phase (PubMed:10588895, PubMed:12970193, PubMed:14741370, PubMed:15123690, PubMed:16341089, PubMed:16431909, PubMed:23615440, PubMed:25358379). Also involved in production of a sulfur precursor required for thiolation of cytoplasmic tRNAs (PubMed:31040179).", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020#function" }, { "database": "PubMed", "id": "15123690", "url": "https://glygen.org/publication/PubMed/15123690" }, { "database": "PubMed", "id": "23615440", "url": "https://glygen.org/publication/PubMed/23615440" }, { "database": "PubMed", "id": "25358379", "url": "https://glygen.org/publication/PubMed/25358379" }, { "database": "PubMed", "id": "14741370", "url": "https://glygen.org/publication/PubMed/14741370" }, { "database": "PubMed", "id": "16431909", "url": "https://glygen.org/publication/PubMed/16431909" }, { "database": "PubMed", "id": "12970193", "url": "https://glygen.org/publication/PubMed/12970193" }, { "database": "PubMed", "id": "16341089", "url": "https://glygen.org/publication/PubMed/16341089" }, { "database": "PubMed", "id": "31040179", "url": "https://glygen.org/publication/PubMed/31040179" }, { "database": "PubMed", "id": "10588895", "url": "https://glygen.org/publication/PubMed/10588895" } ] } ], "disease": [ { "disease_id": "DOID:10591", "recommended_name": { "id": "DOID:10591", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:10591", "name": "pre-eclampsia", "description": "A hypertension occurring during pregnancy characterized by large amounts of protein in the urine (proteinuria) and edema, usually by the last trimester of pregnancy." }, "synonyms": [ { "id": "DOID:10591", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:10591", "name": "gestational hypertension", "description": "" }, { "id": "DOID:10591", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:10591", "name": "hypertension induced by pregnancy", "description": "" }, { "id": "DOID:10591", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:10591", "name": "pre-eclamptic toxaemia", "description": "" }, { "id": "DOID:10591", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:10591", "name": "preeclampsia", "description": "" }, { "id": "DOID:10591", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:10591", "name": "preeclampsia/eclampsia", "description": "" }, { "id": "DOID:10591", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:10591", "name": "pregnancy associated hypertension", "description": "" }, { "id": "DOID:10591", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:10591", "name": "pregnancy toxemia", "description": "" }, { "id": "DOID:10591", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:10591", "name": "proteinuric hypertension of pregnancy", "description": "" }, { "id": "DOID:10591", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:10591", "name": "toxaemia of pregnancy", "description": "" }, { "id": "MONDO:0012266", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012266", "name": "preeclampsia/eclampsia 4", "description": "Any preeclampsia in which the cause of the disease is a mutation in the STOX1 gene." }, { "id": "MONDO:0012266", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012266", "name": "Preeclampsia/eclampsia type 4", "description": "" }, { "id": "MONDO:0012266", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012266", "name": "STOX1 preeclampsia", "description": "" }, { "id": "MONDO:0012266", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012266", "name": "preeclampsia caused by mutation in STOX1", "description": "" }, { "id": "MONDO:0012266", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012266", "name": "preeclampsia/eclampsia 4", "description": "" }, { "id": "MONDO:0012266", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012266", "name": "PEE4", "description": "" }, { "id": "MONDO:0012266", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012266", "name": "PREECLAMPSIA/eclampsia 4", "description": "" }, { "id": "MONDO:0012265", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012265", "name": "preeclampsia/eclampsia 3", "description": "" }, { "id": "MONDO:0012265", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012265", "name": "PEE3", "description": "" }, { "id": "MONDO:0012265", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012265", "name": "PREECLAMPSIA/eclampsia 3", "description": "" }, { "id": "MONDO:0012264", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012264", "name": "preeclampsia/eclampsia 2", "description": "" }, { "id": "MONDO:0012264", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012264", "name": "PEE2", "description": "" }, { "id": "MONDO:0012264", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0012264", "name": "PREECLAMPSIA/eclampsia 2", "description": "" }, { "id": "MONDO:0100467", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0100467", "name": "preeclampsia/eclampsia 1", "description": "" }, { "id": "MONDO:0100467", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0100467", "name": "toxaemia of pregnancy", "description": "" }, { "id": "MONDO:0100467", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0100467", "name": "toxemia of pregnancy", "description": "" }, { "id": "MONDO:0100467", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0100467", "name": "PEE1", "description": "" }, { "id": "MONDO:0100467", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0100467", "name": "PREECLAMPSIA/eclampsia 1", "description": "" }, { "id": "MONDO:0100467", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0100467", "name": "PREG1", "description": "" }, { "id": "MONDO:0100467", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0100467", "name": "hemolysis, elevated liver enzymes, and low platelet count", "description": "" }, { "id": "MONDO:0100467", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0100467", "name": "hypertension, pregnancy-induced", "description": "" }, { "id": "MONDO:0013815", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0013815", "name": "bent bone dysplasia syndrome 1", "description": "" }, { "id": "MONDO:0013815", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0013815", "name": "FGFR2-related bent bone dysplasia", "description": "" }, { "id": "MONDO:0013815", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0013815", "name": "perinatal lethal bent bone dysplasia", "description": "" }, { "id": "MONDO:0013815", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0013815", "name": "BBDS", "description": "" }, { "id": "MONDO:0013815", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0013815", "name": "bent bone dysplasia (BBD)-FGFR2 type", "description": "" }, { "id": "MONDO:0013815", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0013815", "name": "bent bone dysplasia syndrome", "description": "" }, { "id": "MIM:609404", "resource": "MIM", "url": "https://www.omim.org/entry/609404", "name": "Pre-eclampsia/eclampsia 4", "description": "Pre-eclampsia/eclampsia 4" }, { "id": "MIM:614592", "resource": "MIM", "url": "https://www.omim.org/entry/614592", "name": "Bent bone dysplasia syndrome 1", "description": "Bent bone dysplasia syndrome 1" } ], "evidence": [ { "id": "10591", "database": "Alliance of Genome Resources", "url": "https://www.alliancegenome.org/disease/DOID:10591" } ] }, { "disease_id": "DOID:4467", "recommended_name": { "id": "DOID:4467", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:4467", "name": "clear cell renal cell carcinoma", "description": "A renal cell carcinoma that has_material_basis_in cells that appear very pale or clear when examined under microscope." }, "synonyms": [ { "id": "DOID:4467", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:4467", "name": "Clear cell carcinoma of kidney", "description": "" }, { "id": "DOID:4467", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:4467", "name": "clear cell kidney carcinoma", "description": "" }, { "id": "DOID:4467", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:4467", "name": "conventional (Clear cell) renal cell carcinoma", "description": "" }, { "id": "DOID:4467", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:4467", "name": "conventional renal cell carcinoma", "description": "" }, { "id": "DOID:4467", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:4467", "name": "renal clear cell carcinoma", "description": "" } ], "evidence": [ { "id": "4467", "database": "Alliance of Genome Resources", "url": "https://www.alliancegenome.org/disease/DOID:4467" } ] }, { "disease_id": "DOID:699", "recommended_name": { "id": "DOID:699", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:699", "name": "mitochondrial myopathy", "description": "A myopathy that is characterized by mitochondrial dysfunction." }, "synonyms": [ { "id": "DOID:699", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:699", "name": "mitochondrial cytopathy", "description": "" }, { "id": "MONDO:0020714", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0020714", "name": "mitochondrial myopathy, episodic, with optic atrophy and reversible leukoencephalopathy", "description": "" }, { "id": "MIM:251900", "resource": "MIM", "url": "https://www.omim.org/entry/251900", "name": "Mitochondrial myopathy, episodic, with optic atrophy and reversible leukoencephalopathy", "description": "Mitochondrial myopathy, episodic, with optic atrophy and reversible leukoencephalopathy" } ], "evidence": [ { "id": "699", "database": "Alliance of Genome Resources", "url": "https://www.alliancegenome.org/disease/DOID:699" } ] } ], "snv": [], "mutagenesis": [ { "start_pos": 132, "end_pos": 132, "sequence_org": "L", "sequence_mut": "A", "comment": "No growth.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" }, { "database": "PubMed", "id": "15123690", "url": "https://glygen.org/publication/PubMed/15123690" } ] }, { "start_pos": 133, "end_pos": 133, "sequence_org": "P", "sequence_mut": "A", "comment": "Wild-type growth.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" }, { "database": "PubMed", "id": "15123690", "url": "https://glygen.org/publication/PubMed/15123690" } ] }, { "start_pos": 134, "end_pos": 134, "sequence_org": "P", "sequence_mut": "A", "comment": "Slow growth; no interaction with SSQ1.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" }, { "database": "PubMed", "id": "15123690", "url": "https://glygen.org/publication/PubMed/15123690" } ] }, { "start_pos": 134, "end_pos": 136, "sequence_org": "PVK", "sequence_mut": "AAA", "comment": "No growth; no interaction with frataxin and SSQ1.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" }, { "database": "PubMed", "id": "15123690", "url": "https://glygen.org/publication/PubMed/15123690" }, { "database": "PubMed", "id": "25228696", "url": "https://glygen.org/publication/PubMed/25228696" } ] }, { "start_pos": 135, "end_pos": 135, "sequence_org": "V", "sequence_mut": "A", "comment": "Wild-type growth; no interaction with SSQ1.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" }, { "database": "PubMed", "id": "15123690", "url": "https://glygen.org/publication/PubMed/15123690" } ] }, { "start_pos": 136, "end_pos": 136, "sequence_org": "K", "sequence_mut": "A", "comment": "No growth; no interaction with SSQ1.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" }, { "database": "PubMed", "id": "15123690", "url": "https://glygen.org/publication/PubMed/15123690" } ] }, { "start_pos": 139, "end_pos": 139, "sequence_org": "C", "sequence_mut": "A", "comment": "Fails to complement an isu1 deletion mutation.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" }, { "database": "PubMed", "id": "10588895", "url": "https://glygen.org/publication/PubMed/10588895" } ] }, { "start_pos": 141, "end_pos": 141, "sequence_org": "M", "sequence_mut": "E", "comment": "Disrupts iron-sulfur (Fe-S) cluster assembly.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" }, { "database": "PubMed", "id": "31040179", "url": "https://glygen.org/publication/PubMed/31040179" } ] }, { "start_pos": 63, "end_pos": 63, "sequence_org": "L", "sequence_mut": "S", "comment": "In ISU1(LVF/SSS); no growth and abolishes interaction with both JAC1 and NFS1; when associated with S-72 and S-94.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" }, { "database": "PubMed", "id": "10588895", "url": "https://glygen.org/publication/PubMed/10588895" } ] }, { "start_pos": 69, "end_pos": 69, "sequence_org": "C", "sequence_mut": "A", "comment": "Fails to complement an isu1 deletion mutation.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" }, { "database": "PubMed", "id": "10588895", "url": "https://glygen.org/publication/PubMed/10588895" } ] }, { "start_pos": 72, "end_pos": 72, "sequence_org": "V", "sequence_mut": "S", "comment": "In ISU1(LVF/SSS); no growth and abolishes interaction with both JAC1 and NFS1; when associated with S-63 and S-94.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" }, { "database": "PubMed", "id": "10588895", "url": "https://glygen.org/publication/PubMed/10588895" } ] }, { "start_pos": 94, "end_pos": 94, "sequence_org": "F", "sequence_mut": "S", "comment": "In ISU1(LVF/SSS); no growth and abolishes interaction with both JAC1 and NFS1; when associated with S-63 and S-72.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" }, { "database": "PubMed", "id": "10588895", "url": "https://glygen.org/publication/PubMed/10588895" } ] }, { "start_pos": 96, "end_pos": 96, "sequence_org": "C", "sequence_mut": "A", "comment": "Fails to complement an isu1 deletion mutation.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" }, { "database": "PubMed", "id": "10588895", "url": "https://glygen.org/publication/PubMed/10588895" } ] } ], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Saccharomyces cerevisiae S288C", "taxid": 559292, "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "common_name": "Yeast" } ], "gene": [ { "name": "ISU1", "url": "https://www.uniprot.org/uniprot/Q03020", "locus": { "chromosome": "XVI", "start_pos": 298050, "end_pos": 297553, "strand": "-", "evidence": [ { "database": "Ensembl Gene", "id": "YPL135W", "url": "https://www.ensembl.org/id/YPL135W" } ] } } ], "crossref": [ { "id": "cd06664", "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd06664", "database": "CDD", "categories": [ "Protein Classification" ] }, { "id": "855968", "url": "https://www.ncbi.nlm.nih.gov/gene/855968", "database": "GeneID", "categories": [ "Genome Annotation" ] }, { "id": "Q03020", "url": "https://www.ebi.ac.uk/intact/interactors/id:Q03020*", "database": "IntAct", "categories": [ "Other" ] }, { "id": "IPR002871", "url": "https://www.ebi.ac.uk/interpro/entry/IPR002871", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR011339", "url": "https://www.ebi.ac.uk/interpro/entry/IPR011339", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "sce:YPL135W", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+sce:YPL135W", "database": "KEGG Pathway", "categories": [ "Enzyme and Pathway" ] }, { "id": "HKKVIDH", "url": "https://omabrowser.org/oma/group/HKKVIDH/", "database": "OMA", "categories": [ "Protein Classification" ] }, { "id": "151610at2759", "url": "http://www.orthodb.org/?query=151610at2759", "database": "OrthoDB", "categories": [ "Protein Classification" ] }, { "id": "PTHR10093", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR10093", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PTHR10093:SF8", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR10093:SF8", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "5T0V", "url": "https://www.rcsb.org/structure/5T0V", "database": "PDB", "categories": [ "3D Structure" ] }, { "id": "5TRE", "url": "https://www.rcsb.org/structure/5TRE", "database": "PDB", "categories": [ "3D Structure" ] }, { "id": "PF01592", "url": "http://pfam.xfam.org/family/PF01592", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PR_Q03020", "url": "http://purl.obolibrary.org/obo/PR_Q03020", "database": "PRO", "categories": [ "Protein" ] }, { "id": "NP_015190", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_015190", "database": "RefSeq", "categories": [ "Protein" ] }, { "id": "S000006056", "url": "https://www.yeastgenome.org/locus/S000006056", "database": "SGD", "categories": [ "Organism-specific" ] }, { "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "Q03020-1", "url": "https://www.uniprot.org/uniprot/Q03020-1", "database": "UniProtKB", "categories": [ "Protein" ] } ], "sequence": { "sequence": "MLPVITRFARPALMAIRPVNAMGVLRASSITKRLYHPKVIEHYTHPRNVGSLDKKLPNVGTGLVGAPACGDVMRLQIKVNDSTGVIEDVKFKTFGCGSAIASSSYMTELVQGMTLDDAAKIKNTEIAKELSLPPVKLHCSMLAEDAIKAAIKDYKSKRNTPTMLS", "length": 165, "header": "sp|Q03020-1|ISU1_YEAST Isoform 1 of Iron sulfur cluster assembly protein 1, mitochondrial OS=Saccharomyces cerevisiae S288C OX=559292 GN=ISU1 PE=1 SV=1 CANONICAL=Q03020-1" }, "isoforms": [ { "isoform_ac": "Q03020-1", "url": "", "sequence": { "sequence": "MLPVITRFARPALMAIRPVNAMGVLRASSITKRLYHPKVIEHYTHPRNVGSLDKKLPNVGTGLVGAPACGDVMRLQIKVNDSTGVIEDVKFKTFGCGSAIASSSYMTELVQGMTLDDAAKIKNTEIAKELSLPPVKLHCSMLAEDAIKAAIKDYKSKRNTPTMLS", "length": 165, "header": "sp|Q03020-1|ISU1_YEAST Isoform 1 of Iron sulfur cluster assembly protein 1, mitochondrial OS=Saccharomyces cerevisiae S288C OX=559292 GN=ISU1 PE=1 SV=1 CANONICAL=Q03020-1" }, "locus": { "chromosome": "XVI", "start_pos": 298047, "end_pos": 297553, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "YPL135W_mRNA", "url": "https://www.ensembl.org/id/YPL135W_mRNA" }, { "database": "Ensembl Peptide", "id": "YPL135W", "url": "https://www.ensembl.org/id/YPL135W" } ] } } ], "glycosylation": [], "phosphorylation": [], "glycation": [], "keywords": [], "publication": [ { "title": "Evidence for a conserved system for iron metabolism in the mitochondria of Saccharomyces cerevisiae.", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "date": "1999", "authors": "Beinert H., Schilke B., Voisine C., Craig E.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "10468587", "url": "https://glygen.org/publication/PubMed/10468587" } ] }, { "title": "Saccharomyces cerevisiae ISU1 and ISU2: members of a well-conserved gene family for iron-sulfur cluster assembly.", "journal": "J. Mol. Biol.", "date": "1999", "authors": "Garland S A, Hoff K, Vickery L E, Culotta V C", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "10588895", "url": "https://glygen.org/publication/PubMed/10588895" } ] }, { "title": "Ssq1, a mitochondrial Hsp70 involved in iron-sulfur (Fe/S) center biogenesis. Similarities to and differences from its bacterial counterpart.", "journal": "J. Biol. Chem.", "date": "2003", "authors": "Craig E.A., Walter W., Dutkiewicz R., Knieszner H., Marszalek J., Schilke B.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "12756240", "url": "https://glygen.org/publication/PubMed/12756240" } ] }, { "title": "An interaction between frataxin and Isu1/Nfs1 that is crucial for Fe/S cluster synthesis on Isu1.", "journal": "EMBO Rep.", "date": "2003", "authors": "Gerber J, Muhlenhoff U, Lill R", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "12947415", "url": "https://glygen.org/publication/PubMed/12947415" } ] }, { "title": "Components involved in assembly and dislocation of iron-sulfur clusters on the scaffold protein Isu1p.", "journal": "EMBO J.", "date": "2003", "authors": "Muhlenhoff U, Gerber J, Richhardt N, Lill R", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "12970193", "url": "https://glygen.org/publication/PubMed/12970193" } ] }, { "title": "Global analysis of protein expression in yeast.", "journal": "Nature", "date": "2003", "authors": "Dephoure N., Weissman J.S., Howson R.W., Huh W.-K., O'Shea E.K., Belle A., Bower K., Ghaemmaghami S.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "14562106", "url": "https://glygen.org/publication/PubMed/14562106" } ] }, { "title": "Mitochondrial functional interactions between frataxin and Isu1p, the iron-sulfur cluster scaffold protein, in Saccharomyces cerevisiae.", "journal": "FEBS Lett.", "date": "2004", "authors": "Foury F., Ramazzotti A., Vanmansart V.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "14741370", "url": "https://glygen.org/publication/PubMed/14741370" } ] }, { "title": "Sequence-specific interaction between mitochondrial Fe-S scaffold protein Isu and Hsp70 Ssq1 is essential for their in vivo function.", "journal": "J. Biol. Chem.", "date": "2004", "authors": "Dutkiewicz Rafal, Schilke Brenda, Cheng Sara, Knieszner Helena, Craig Elizabeth A, Marszalek Jaroslaw", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "15123690", "url": "https://glygen.org/publication/PubMed/15123690" } ] }, { "title": "The yeast scaffold proteins Isu1p and Isu2p are required inside mitochondria for maturation of cytosolic Fe/S proteins.", "journal": "Mol. Cell. Biol.", "date": "2004", "authors": "Gerber J., Lill R., Muehlenhoff U., Neumann K., Prohl C.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "15143178", "url": "https://glygen.org/publication/PubMed/15143178" } ] }, { "title": "Essential role of Isd11 in mitochondrial iron-sulfur cluster synthesis on Isu scaffold proteins.", "journal": "EMBO J.", "date": "2006", "authors": "Ryan M.T., Lill R., Mueller H., Guiard B., Meyer H.E., Muehlenhoff U., Meisinger C., Lohaus C., Pfanner N., Urzica E., Wiedemann N.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "16341089", "url": "https://glygen.org/publication/PubMed/16341089" } ] }, { "title": "The Hsp70 chaperone Ssq1p is dispensable for iron-sulfur cluster formation on the scaffold protein Isu1p.", "journal": "J. Biol. Chem.", "date": "2006", "authors": "Lill R., Muehlenhoff U., Craig E.A., Dutkiewicz R., Marszalek J., Schilke B.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "16431909", "url": "https://glygen.org/publication/PubMed/16431909" } ] }, { "title": "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics.", "journal": "J. Proteome Res.", "date": "2006", "authors": "Sickmann A., Zahedi R.P., Meisinger C., Reinders J., Pfanner N.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "16823961", "url": "https://glygen.org/publication/PubMed/16823961" } ] }, { "title": "Molecular details of the yeast frataxin-Isu1 interaction during mitochondrial Fe-S cluster assembly.", "journal": "Biochemistry", "date": "2010", "authors": "Dancis A., Kondapalli K.C., Cook J.D., Stemmler T.L., Rawat S., Childs W.C., Murugesan Y.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "20815377", "url": "https://glygen.org/publication/PubMed/20815377" } ] }, { "title": "Interaction of J-protein co-chaperone Jac1 with Fe-S scaffold Isu is indispensable in vivo and conserved in evolution.", "journal": "J. Mol. Biol.", "date": "2012", "authors": "Ciesielski SJ, Schilke BA, Osipiuk J, Bigelow L, Mulligan R, Majewska J, Joachimiak A, Marszalek J, Craig EA, Dutkiewicz R", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "22306468", "url": "https://glygen.org/publication/PubMed/22306468" } ] }, { "title": "The mitochondrial Hsp70 chaperone Ssq1 facilitates Fe/S cluster transfer from Isu1 to Grx5 by complex formation.", "journal": "Mol. Biol. Cell", "date": "2013", "authors": "Lill R., Muehlenhoff U., Uzarska M.A., Dutkiewicz R., Freibert S.A.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "23615440", "url": "https://glygen.org/publication/PubMed/23615440" } ] }, { "title": "Binding of the chaperone Jac1 protein and cysteine desulfurase Nfs1 to the iron-sulfur cluster scaffold Isu protein is mutually exclusive.", "journal": "J. Biol. Chem.", "date": "2013", "authors": "Craig E.A., Ciesielski S.J., Song J.Y., Dutkiewicz R., Blenska A., Delewski W., Kominek J., Majewska J., Marszalek J., Schilke B.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "23946486", "url": "https://glygen.org/publication/PubMed/23946486" } ] }, { "title": "The reference genome sequence of Saccharomyces cerevisiae: Then and now.", "journal": "G3 (Bethesda)", "date": "2014", "authors": "Engel Stacia R, Dietrich Fred S, Fisk Dianna G, Binkley Gail, Balakrishnan Rama, Costanzo Maria C, Dwight Selina S, Hitz Benjamin C, Karra Kalpana, Nash Robert S, Weng Shuai, Wong Edith D, Lloyd Paul, Skrzypek Marek S, Miyasato Stuart R, Simison Matt, Cherry J Michael", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "24374639", "url": "https://glygen.org/publication/PubMed/24374639" } ] }, { "title": "Overlapping binding sites of the frataxin homologue assembly factor and the heat shock protein 70 transfer factor on the Isu iron-sulfur cluster scaffold protein.", "journal": "J. Biol. Chem.", "date": "2014", "authors": "Manicki Mateusz, Majewska Julia, Ciesielski Szymon, Schilke Brenda, Blenska Anna, Kominek Jacek, Marszalek Jaroslaw, Craig Elizabeth A, Dutkiewicz Rafal", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "25228696", "url": "https://glygen.org/publication/PubMed/25228696" } ] }, { "title": "Functional reconstitution of mitochondrial Fe/S cluster synthesis on Isu1 reveals the involvement of ferredoxin.", "journal": "Nat. Commun.", "date": "2014", "authors": "Lill R., Webert H., Banci L., Gallo A., Muehlenhoff U., Hurt E., Freibert S.A., Amlacher S., Heidenreich T., Linne U.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "25358379", "url": "https://glygen.org/publication/PubMed/25358379" } ] }, { "title": "Molecular modeling of the binding modes of the iron-sulfur protein to the Jac1 co-chaperone from Saccharomyces cerevisiae by all-atom and coarse-grained approaches.", "journal": "Proteins", "date": "2015", "authors": "Scheraga H.A., Krupa P., Liwo A., Mozolewska M.A.", "evidence": [ { "database": "UniProtKB", "id": "Q03020", "url": "https://www.uniprot.org/uniprot/Q03020" } ], "reference": [ { "type": "PubMed", "id": "25973573", "url": "https://glygen.org/publication/PubMed/25973573" } ] }, { "title": "Mitochondria export iron-sulfur and sulfur intermediates to the cytoplasm for iron-sulfur cluster assembly and tRNA thiolation in yeast.", "journal": "J. 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