{ "uniprot_canonical_ac": "P40085-1", "uniprot_ac": "P40085", "uniprot_id": "TAPT1_YEAST", "mass": { "chemical_mass": 64794.0, "monoisotopic_mass": 64794.0 }, "refseq": { "ac": "NP_011067", "name": "Emp65p", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_011067" }, "gene_names": [ { "name": "EMP65", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/P40085", "id": "P40085" }, { "name": "EMP65", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_011067", "id": "NP_011067" } ], "protein_names": [ { "name": "Endoplasmic reticulum membrane protein 65", "resource": "UniProtKB", "type": "recommended", "url": "", "id": "P40085" }, { "name": "65 kDa endoplasmic reticulum membrane protein", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "P40085" }, { "name": "Emp65p", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_011067", "id": "NP_011067" } ], 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"IPR008010", "url": "https://www.ebi.ac.uk/interpro/entry/IPR008010", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "sce:YER140W", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+sce:YER140W", "database": "KEGG Pathway", "categories": [ "Enzyme and Pathway" ] }, { "id": "TSRVYHR", "url": "https://omabrowser.org/oma/group/TSRVYHR/", "database": "OMA", "categories": [ "Protein Classification" ] }, { "id": "1215898at2759", "url": "http://www.orthodb.org/?query=1215898at2759", "database": "OrthoDB", "categories": [ "Protein Classification" ] }, { "id": "PTHR13317", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR13317", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PTHR13317:SF4", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR13317:SF4", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PF05346", "url": "http://pfam.xfam.org/family/PF05346", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PR_P40085", "url": "http://purl.obolibrary.org/obo/PR_P40085", "database": "PRO", "categories": [ "Protein" ] }, { "id": "NP_011067", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_011067", "database": "RefSeq", "categories": [ "Protein" ] }, { "id": "S000000942", "url": "https://www.yeastgenome.org/locus/S000000942", "database": "SGD", "categories": [ "Organism-specific" ] }, { "id": "P40085", "url": "https://www.uniprot.org/uniprot/P40085", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "P40085-1", "url": "https://www.uniprot.org/uniprot/P40085-1", "database": "UniProtKB", "categories": [ "Protein" ] } ], "sequence": { "sequence": "MQHKDTAVAKDTAKKRLLRRNSAPSAIHIISRLDKKWSFLWNTIDRHNIVEEQDESSAAKSEEEHEDDYELEQLLNMIRIPMFLEKFMLFALLTSLDCFLYYFTVLPIRLIKGYVKQFKSYRQHYRLQQRSGHKNKIPFRYRITSREYKERCMIFIIVISSILLSKLDTSKLYHRIKRQSTMKLYMLFSVLEMADKMLASLGQSLLTVMLSRKNSERILLHKCLLVSMSLTYVTIHGYVLVYQAISLNIAVNSYSNALLTLLLSMQFAEIKSSVLKKFDKEGFFQITIADVVERFKLTLLLSITGLRNLQSWSSSLSNTSINFWSPRSTLSIVINILCGPMVSVVGSEVLVDWAKHAYITKFNRIRPQIYDKFYYIIYKDYSTRTHKLEDRLGLPLPAFVVLFIVMVRPTLFKSSEPSYLPSLFRILFMGASVFLLALLAKFTLDLILIKWSKRIEQRFRDQAFNTVVTEEEYVPGLLSGGMGKVDVSTRIALHSDYNKENRIETESVSPMRKRKTTLTAECTPPSLNDIRRQKDSKNPRSLENVARYKMVSKRIW", "length": 556, "header": "sp|P40085-1|TAPT1_YEAST Isoform 1 of Endoplasmic reticulum membrane protein 65 OS=Saccharomyces cerevisiae S288C OX=559292 GN=EMP65 PE=1 SV=1 CANONICAL=P40085-1" }, "isoforms": [ { "isoform_ac": "P40085-1", "url": "", "sequence": { "sequence": "MQHKDTAVAKDTAKKRLLRRNSAPSAIHIISRLDKKWSFLWNTIDRHNIVEEQDESSAAKSEEEHEDDYELEQLLNMIRIPMFLEKFMLFALLTSLDCFLYYFTVLPIRLIKGYVKQFKSYRQHYRLQQRSGHKNKIPFRYRITSREYKERCMIFIIVISSILLSKLDTSKLYHRIKRQSTMKLYMLFSVLEMADKMLASLGQSLLTVMLSRKNSERILLHKCLLVSMSLTYVTIHGYVLVYQAISLNIAVNSYSNALLTLLLSMQFAEIKSSVLKKFDKEGFFQITIADVVERFKLTLLLSITGLRNLQSWSSSLSNTSINFWSPRSTLSIVINILCGPMVSVVGSEVLVDWAKHAYITKFNRIRPQIYDKFYYIIYKDYSTRTHKLEDRLGLPLPAFVVLFIVMVRPTLFKSSEPSYLPSLFRILFMGASVFLLALLAKFTLDLILIKWSKRIEQRFRDQAFNTVVTEEEYVPGLLSGGMGKVDVSTRIALHSDYNKENRIETESVSPMRKRKTTLTAECTPPSLNDIRRQKDSKNPRSLENVARYKMVSKRIW", "length": 556, "header": "sp|P40085-1|TAPT1_YEAST Isoform 1 of Endoplasmic reticulum membrane protein 65 OS=Saccharomyces cerevisiae S288C OX=559292 GN=EMP65 PE=1 SV=1 CANONICAL=P40085-1" }, "locus": { "chromosome": "V", "start_pos": 453232, "end_pos": 451565, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "YER140W_mRNA", "url": "https://www.ensembl.org/id/YER140W_mRNA" }, { "database": "Ensembl Peptide", "id": "YER140W", "url": "https://www.ensembl.org/id/YER140W" } ] } } ], "glycosylation": [ { "glytoucan_ac": "", "type": "N-linked", "subtype": "", "site_category": "reported", "site_seq": "N", "relation": "attached", "comment": "N-linked (GlcNAc...) asparagine", "start_pos": 318, "start_aa": "Asn", "end_pos": 318, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn318", "evidence": [ { "id": "P40085", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P40085#ptm_processing" }, { "id": "PRU00498", "database": "PROSITE-PRORULE", "url": "https://prosite.expasy.org/rule/PRU00498" } ] } ], "phosphorylation": [ { "start_pos": 22, "end_pos": 22, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "Phosphoserine", "evidence": [ { "database": "UniProtKB", "id": "P40085", "url": "https://www.uniprot.org/uniprot/P40085" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "PubMed", "id": "17330950", "url": "https://glygen.org/publication/PubMed/17330950" }, { "database": "iPTMnet", "id": "P40085", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P40085" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" }, { "database": "SGD", "id": "P40085", "url": "https://www.yeastgenome.org/locus/P40085" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ], "site_lbl": "Ser22" }, { "start_pos": 509, "end_pos": 509, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "SGD", "id": "P40085", "url": "https://www.yeastgenome.org/locus/P40085" }, { "database": "iPTMnet", "id": "P40085", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P40085" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ], "site_lbl": "Ser509" } ], "glycation": [], "keywords": [ "glycoprotein", "glycoprotein_reported" ], "publication": [ { "title": "The proteome of Saccharomyces cerevisiae mitochondria.", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "date": "2003", "authors": "Sickmann A., Zahedi R.P., Rehling P., Chacinska A., Guiard B., Meyer H.E., Meisinger C., Reinders J., Pfanner N., Joppich C., Perschil I., Schoenfisch B., Wagner Y.", "evidence": [ { "database": "UniProtKB", "id": "P40085", "url": "https://www.uniprot.org/uniprot/P40085" } ], "reference": [ { "type": "PubMed", "id": "14576278", "url": "https://glygen.org/publication/PubMed/14576278" } ] }, { "title": "A global topology map of the Saccharomyces cerevisiae membrane proteome.", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "date": "2006", "authors": "Kim H., von Heijne G., Melen K., Oesterberg M.", "evidence": [ { "database": "UniProtKB", "id": "P40085", "url": "https://www.uniprot.org/uniprot/P40085" } ], "reference": [ { "type": "PubMed", "id": "16847258", "url": "https://glygen.org/publication/PubMed/16847258" } ] }, { "title": "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.", "journal": "Journal of proteome research", "date": "2007-03", "authors": "Li Xue, Gerber Scott A, Rudner Adam D, Beausoleil Sean A, Haas Wilhelm, Vill\u00e9n Judit, Elias Joshua E, Gygi Steve P", "evidence": [ { "database": "UniProtKB", "id": "P40085", "url": "https://www.uniprot.org/uniprot/P40085" }, { "database": "iPTMnet", "id": "P40085", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P40085" } ], "reference": [ { "type": "PubMed", "id": "17330950", "url": "https://glygen.org/publication/PubMed/17330950" } ] }, { "title": "A multidimensional chromatography technology for in-depth phosphoproteome analysis.", "journal": "Mol. Cell. Proteomics", "date": "2008", "authors": "Albuquerque Claudio P, Smolka Marcus B, Payne Samuel H, Bafna Vineet, Eng Jimmy, Zhou Huilin", "evidence": [ { "database": "UniProtKB", "id": "P40085", "url": "https://www.uniprot.org/uniprot/P40085" }, { "database": "iPTMnet", "id": "P40085", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P40085" } ], "reference": [ { "type": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" } ] }, { "title": "Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum.", "journal": "Science", "date": "2009", "authors": "Schwappach B., Oh E., Schuldiner M., Walter P., Weissman J.S., Collins S.R., Denic V., Schmid V., Jonikas M.C., Quan E.M., Weibezahn J.", "evidence": [ { "database": "UniProtKB", "id": "P40085", "url": "https://www.uniprot.org/uniprot/P40085" } ], "reference": [ { "type": "PubMed", "id": "19325107", "url": "https://glygen.org/publication/PubMed/19325107" } ] }, { "title": "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.", "journal": "Science (New York, N.Y.)", "date": "2009-09-25", "authors": "Holt Liam J, Tuch Brian B, Vill\u00e9n Judit, Johnson Alexander D, Gygi Steven P, Morgan David O", "evidence": [ { "database": "UniProtKB", "id": "P40085", "url": "https://www.uniprot.org/uniprot/P40085" }, { "database": "iPTMnet", "id": "P40085", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P40085" } ], "reference": [ { "type": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" } ] }, { "title": "Genetic analysis of Mps3 SUN domain mutants in Saccharomyces cerevisiae reveals an interaction with the SUN-like protein Slp1.", "journal": "G3 (Bethesda)", "date": "2012", "authors": "Gardner J.M., Jaspersen S.L., Slaughter B.D., Gogol M., Friederichs J.M., Smoyer C.J., Whetstine C.R.", "evidence": [ { "database": "UniProtKB", "id": "P40085", "url": "https://www.uniprot.org/uniprot/P40085" } ], "reference": [ { "type": "PubMed", "id": "23275891", "url": "https://glygen.org/publication/PubMed/23275891" } ] }, { "title": "Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.", "journal": "Nature methods", "date": "2013-07", "authors": "Swaney Danielle L, Beltrao Pedro, Starita Lea, Guo Ailan, Rush John, Fields Stanley, Krogan Nevan J, Vill\u00e9n Judit", "evidence": [ { "database": "iPTMnet", "id": "P40085", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P40085" } ], "reference": [ { "type": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ] }, { "title": "The reference genome sequence of Saccharomyces cerevisiae: Then and now.", "journal": "G3 (Bethesda)", "date": "2014", "authors": "Engel Stacia R, Dietrich Fred S, Fisk Dianna G, Binkley Gail, Balakrishnan Rama, Costanzo Maria C, Dwight Selina S, Hitz Benjamin C, Karra Kalpana, Nash Robert S, Weng Shuai, Wong Edith D, Lloyd Paul, Skrzypek Marek S, Miyasato Stuart R, Simison Matt, Cherry J Michael", "evidence": [ { "database": "UniProtKB", "id": "P40085", "url": "https://www.uniprot.org/uniprot/P40085" } ], "reference": [ { "type": "PubMed", "id": "24374639", "url": "https://glygen.org/publication/PubMed/24374639" } ] }, { "title": "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.", "journal": "Nature", "date": "1997", "authors": "Smith V., Chen E., Guzman E., Chung E., Nakahara K., Botstein D., Davis R.W., Lin D., Wei Y., Duncan M., Berno A., Oh C., Oefner P., Taylor P., Lashkari D., Allen E., Hyman R.W., Araujo R., Komp C., Lew H., Namath A., Roberts D., Mulligan J.T., Sehl P., Schramm S., Hartzell G., Cherry J.M., Dietrich F.S., Aviles E., Brennan T., Carpenter J., Hennessy K.M., Hunicke-Smith S., Kayser A., Mosedale D., Norgren R., Petel F.X., Shogren T., Yelton M.A.", "evidence": [ { "database": "UniProtKB", "id": "P40085", "url": "https://www.uniprot.org/uniprot/P40085" } ], "reference": [ { "type": "PubMed", "id": "9169868", "url": "https://glygen.org/publication/PubMed/9169868" } ] } ], "pathway": [ { "id": "sce:YER140W", "name": "", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+sce:YER140W", "resource": "KEGG Pathway" } ], "orthologs": [], "go_annotation": { "categories": [ { "name": "Cellular Component", "total": 4, "go_terms": [ { "name": "mitochondrion", "id": "GO:0005739", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005739", "evidence": [ { "database": "UniProtKB", "id": "P40085", "url": "https://www.uniprot.org/uniprot/P40085" } ], "pmid": "" }, { "name": "endoplasmic reticulum", "id": "GO:0005783", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005783", "evidence": [ { "database": "UniProtKB", "id": "P40085", "url": "https://www.uniprot.org/uniprot/P40085" } ], "pmid": "" }, { "name": "endoplasmic reticulum membrane", "id": "GO:0005789", "url": 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