{ "uniprot_canonical_ac": "P38289-1", "uniprot_ac": "P38289", "uniprot_id": "EXO5_YEAST", "mass": { "chemical_mass": 67570.0, "monoisotopic_mass": 67570.0 }, "refseq": { "ac": "NP_009722", "name": "Exo5p", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_009722" }, "gene_names": [ { "name": "EXO5", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/P38289", "id": "P38289" }, { "name": "DEM1", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_009722", "id": "NP_009722" }, { "name": "DEM1", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/P38289", "id": "P38289" }, { "name": "EXO5", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_009722", "id": "NP_009722" }, { "name": "YBR1215", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/P38289", "id": "P38289" } ], "protein_names": [ { "name": "Exonuclease V, mitochondrial", "resource": "UniProtKB", "type": "recommended", "url": "", "id": "P38289" }, { "name": "3.1.-.-", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "P38289" }, { "name": "Defects in morphology protein 1", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "P38289" }, { "name": "Exo V", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "P38289" }, { "name": "Exo5p", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_009722", "id": "NP_009722" } ], "function": [ { "annotation": "Single strand DNA specific 5' exonuclease involved in mitochondrial DNA replication and recombination. Releases dinucleotides as main products of catalysis. Has the capacity to slide across 5'double-stranded DNA or 5'RNA sequences and resumes cutting two nucleotides downstream of the double-stranded-to-single-stranded junction or RNA-to-DNA junction, respectively.", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289#function" }, { "database": "PubMed", "id": "20086101", "url": "https://glygen.org/publication/PubMed/20086101" }, { "database": "PubMed", "id": "3286646", "url": "https://glygen.org/publication/PubMed/3286646" }, { "database": "PubMed", "id": "10628851", "url": "https://glygen.org/publication/PubMed/10628851" } ] } ], "disease": [], "snv": [], "mutagenesis": [ { "start_pos": 270, "end_pos": 270, "sequence_org": "D", "sequence_mut": "A", "comment": "Impairs exonuclease activity.", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" }, { "database": "PubMed", "id": "20086101", "url": "https://glygen.org/publication/PubMed/20086101" } ] }, { "start_pos": 320, "end_pos": 320, "sequence_org": "D", "sequence_mut": "A", "comment": "Impairs exonuclease activity.", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" }, { "database": "PubMed", "id": "20086101", "url": "https://glygen.org/publication/PubMed/20086101" } ] } ], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Saccharomyces cerevisiae S288C", "taxid": 559292, "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" } ], "common_name": "Yeast" } ], "gene": [ { "name": "EXO5", "url": "https://www.uniprot.org/uniprot/P38289", "locus": { "chromosome": "II", "start_pos": 567480, "end_pos": 565723, "strand": "-", "evidence": [ { "database": "Ensembl Gene", "id": "YBR163W", "url": "https://www.ensembl.org/id/YBR163W" } ] } } ], "crossref": [ { "id": "852461", "url": "https://www.ncbi.nlm.nih.gov/gene/852461", "database": "GeneID", "categories": [ "Genome Annotation" ] }, { "id": "P38289", "url": "https://www.ebi.ac.uk/intact/interactors/id:P38289*", "database": "IntAct", "categories": [ "Other" ] }, { "id": "IPR016610", "url": "https://www.ebi.ac.uk/interpro/entry/IPR016610", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR019190", "url": "https://www.ebi.ac.uk/interpro/entry/IPR019190", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "sce:YBR163W", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+sce:YBR163W", "database": "KEGG Pathway", "categories": [ "Enzyme and Pathway" ] }, { "id": "LQVMYYR", "url": "https://omabrowser.org/oma/group/LQVMYYR/", "database": "OMA", "categories": [ "Protein Classification" ] }, { "id": "1111955at2759", "url": "http://www.orthodb.org/?query=1111955at2759", "database": "OrthoDB", "categories": [ "Protein Classification" ] }, { "id": "PTHR14464", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR14464", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PTHR14464:SF4", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR14464:SF4", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PF09810", "url": "http://pfam.xfam.org/family/PF09810", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PR_P38289", "url": "http://purl.obolibrary.org/obo/PR_P38289", "database": "PRO", "categories": [ "Protein" ] }, { "id": "NP_009722", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_009722", "database": "RefSeq", "categories": [ "Protein" ] }, { "id": "S000000367", "url": "https://www.yeastgenome.org/locus/S000000367", "database": "SGD", "categories": [ "Organism-specific" ] }, { "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "P38289-1", "url": "https://www.uniprot.org/uniprot/P38289-1", "database": "UniProtKB", "categories": [ "Protein" ] } ], "sequence": { "sequence": "MLGRALINKYGFLIHPRRFVHLNDKSLDGTFILPSKKNHMYDVPTNDPSGILNASDIDRINNLPFFDNTSPTKETNTKEGALLSEKLASVKELFGEDPENPSFINYRFPRGLENPYFDIQVNQLKKKRLSVTQLCTTQNWCELRNFYDFYSQNLSNQLLNLKFQVQKGKKIHKSLEDETHPELNQYKSFTHNFLALTKLSMDIDNDMDALLDNWFNSINRLVSLFTKGDGHAREIVCHGFINLEDGKLVEHLLNSDSKTKENVIISGVIDHLTLRNRHNHQVQKGAAHLDTEYQSWGNILTNLLSNLKELKSNNEIVISDIKTRSVPKIPSIESVIESSKLQTMYYKFFFSHLSQDMTQTYHSFLINAQRRGLDVDAPINPTKILTFILTNPLFANDVKNLLYGLPINHSAFDNDAKGSNTFDMTAFNDLLDRGPTSFNVPIEQDEDSSESTKCVSLRDYGHFYTKWKTPLTLKYFAARLSQIYFIVGNLVSNDLMIEYYYHNDNFHNIIFPYDPLKLGTHAHDSAMVWFGGRDMHPIEPTQKNFNTYCKFCDYRHVCSWKNKNELKLIDLGKELKKIILESSMK", "length": 585, "header": "sp|P38289-1|EXO5_YEAST Isoform 1 of Exonuclease V, mitochondrial OS=Saccharomyces cerevisiae S288C OX=559292 GN=EXO5 PE=1 SV=1 CANONICAL=P38289-1" }, "isoforms": [ { "isoform_ac": "P38289-1", "url": "", "sequence": { "sequence": "MLGRALINKYGFLIHPRRFVHLNDKSLDGTFILPSKKNHMYDVPTNDPSGILNASDIDRINNLPFFDNTSPTKETNTKEGALLSEKLASVKELFGEDPENPSFINYRFPRGLENPYFDIQVNQLKKKRLSVTQLCTTQNWCELRNFYDFYSQNLSNQLLNLKFQVQKGKKIHKSLEDETHPELNQYKSFTHNFLALTKLSMDIDNDMDALLDNWFNSINRLVSLFTKGDGHAREIVCHGFINLEDGKLVEHLLNSDSKTKENVIISGVIDHLTLRNRHNHQVQKGAAHLDTEYQSWGNILTNLLSNLKELKSNNEIVISDIKTRSVPKIPSIESVIESSKLQTMYYKFFFSHLSQDMTQTYHSFLINAQRRGLDVDAPINPTKILTFILTNPLFANDVKNLLYGLPINHSAFDNDAKGSNTFDMTAFNDLLDRGPTSFNVPIEQDEDSSESTKCVSLRDYGHFYTKWKTPLTLKYFAARLSQIYFIVGNLVSNDLMIEYYYHNDNFHNIIFPYDPLKLGTHAHDSAMVWFGGRDMHPIEPTQKNFNTYCKFCDYRHVCSWKNKNELKLIDLGKELKKIILESSMK", "length": 585, "header": "sp|P38289-1|EXO5_YEAST Isoform 1 of Exonuclease V, mitochondrial OS=Saccharomyces cerevisiae S288C OX=559292 GN=EXO5 PE=1 SV=1 CANONICAL=P38289-1" }, "locus": { "chromosome": "II", "start_pos": 567477, "end_pos": 565723, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "YBR163W_mRNA", "url": "https://www.ensembl.org/id/YBR163W_mRNA" }, { "database": "Ensembl Peptide", "id": "YBR163W", "url": "https://www.ensembl.org/id/YBR163W" } ] } } ], "glycosylation": [], "phosphorylation": [], "glycation": [], "keywords": [ "enzyme" ], "publication": [ { "title": "Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach.", "journal": "Mol. Gen. Genet.", "date": "1999", "authors": "Hansen M., Rose M., Meyer P., Wolf K., Klein S., Feldmann H., Jansen G., Schaefer B., Kramer W., Goetz R., Koetter P., Entian K.-D., Munder T., Launhardt H., Hollenberg C.P., Kricke J., Becher D., Gueldener U., Hegemann J.H., Hinnen A., Mannhaupt G., Schuster T., Zimmermann F.K., Zollner A., Maierl A., Mewes W., Niedenthal R.K., Ramezani Rad M., Roehmer A., Roemer A., Siegler M.-L., Vetter J., Wilhelm N.", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" } ], "reference": [ { "type": "PubMed", "id": "10628851", "url": "https://glygen.org/publication/PubMed/10628851" } ] }, { "title": "Global analysis of protein localization in budding yeast.", "journal": "Nature", "date": "2003", "authors": "Weissman J.S., Carroll A.S., Howson R.W., Huh W.-K., O'Shea E.K., Falvo J.V., Gerke L.C.", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" } ], "reference": [ { "type": "PubMed", "id": "14562095", "url": "https://glygen.org/publication/PubMed/14562095" } ] }, { "title": "Global analysis of protein expression in yeast.", "journal": "Nature", "date": "2003", "authors": "Dephoure N., Weissman J.S., Howson R.W., Huh W.-K., O'Shea E.K., Belle A., Bower K., Ghaemmaghami S.", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" } ], "reference": [ { "type": "PubMed", "id": "14562106", "url": "https://glygen.org/publication/PubMed/14562106" } ] }, { "title": "Yeast exonuclease 5 is essential for mitochondrial genome maintenance.", "journal": "Mol. Cell. Biol.", "date": "2010", "authors": "Burgers Peter M, Stith Carrie M, Yoder Bonita L, Sparks Justin L", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" } ], "reference": [ { "type": "PubMed", "id": "20086101", "url": "https://glygen.org/publication/PubMed/20086101" } ] }, { "title": "The reference genome sequence of Saccharomyces cerevisiae: Then and now.", "journal": "G3 (Bethesda)", "date": "2014", "authors": "Engel Stacia R, Dietrich Fred S, Fisk Dianna G, Binkley Gail, Balakrishnan Rama, Costanzo Maria C, Dwight Selina S, Hitz Benjamin C, Karra Kalpana, Nash Robert S, Weng Shuai, Wong Edith D, Lloyd Paul, Skrzypek Marek S, Miyasato Stuart R, Simison Matt, Cherry J Michael", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" } ], "reference": [ { "type": "PubMed", "id": "24374639", "url": "https://glygen.org/publication/PubMed/24374639" } ] }, { "title": "Exonuclease V from Saccharomyces cerevisiae. A 5'-3'-deoxyribonuclease that produces dinucleotides in a sequential fashion.", "journal": "J. Biol. Chem.", "date": "1988", "authors": "Burgers P.M., Tam L., Bauer G.A.", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" } ], "reference": [ { "type": "PubMed", "id": "3286646", "url": "https://glygen.org/publication/PubMed/3286646" } ] }, { "title": "Complete DNA sequence of yeast chromosome II.", "journal": "EMBO J.", "date": "1994", "authors": "Gassenhuber J., Mewes H.-W., Obermaier B., Stucka R., Rose M., Wagner G., Boles E., Brueckner M., Jacquet M., Rieger M., Mueller S., Molemans F., Zagulski M., Brandt T., Christiansen C., Barthe C., Feldmann H., Kleine K., Cziepluch C., Fiers W., Mallet L., Jauniaux J.-C., Dubois E., Andre B., Li Z.Y., Koetter P., Hein C., Entian K.-D., Slonimski P.P., Vetter I., Nasr F., Louis E.J., Delaveau T., Hollenberg C.P., Contreras R., Domdey H., Pohl T.M., Brendel M., Becam A.-M., Bussereau F., Crouzet M., Doignon F., Dujon B., El Bakkoury M., Feuermann M., Glansdorff N., Goffeau A., Grivell L.A., Herbert C.J., Jacq C., Mannhaupt G., Messenguy F., Miosga T., Perea J., Pierard A., Potier S., Proft M., Schaaff-Gerstenschlaeger I., Scherens B., Souciet J.-L., Steensma H.Y., Vissers S., Zimmermann F.K., Aljinovic G., Duesterhus S., Jonniaux J.-L., Kirchrath L., Purnelle B., Ramezani Rad M., Skala J., Urrestarazu L.A., Vierendeels F., de Wergifosse P., Aigle M., Baclet M.C., Baur A., Biteau N., Demolis N., Fobo G.M., Fritz C., Holmstroem K., Kallesoee T., Kiesau P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Maat M.J., Piravandi E., Pohl F.M., Schwarzlose C., Smits P.H.M., Van Dyck L., Vassarotti A., Wolfe K.H., de Haan M., van der Aart Q.J.M.", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" } ], "reference": [ { "type": "PubMed", "id": "7813418", "url": "https://glygen.org/publication/PubMed/7813418" } ] } ], "pathway": [ { "id": "sce:YBR163W", "name": "", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+sce:YBR163W", "resource": "KEGG Pathway" } ], "orthologs": [], "go_annotation": { "categories": [ { "name": "Molecular Function", "total": 4, "go_terms": [ { "name": "DNA binding", "id": "GO:0003677", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003677", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" } ], "pmid": "" }, { "name": "metal ion binding", "id": "GO:0046872", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046872", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" } ], "pmid": "" }, { "name": "4 iron, 4 sulfur cluster binding", "id": "GO:0051539", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0051539", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" } ], "pmid": "" }, { "name": "single-stranded DNA 5'-3' DNA exonuclease activity", "id": "GO:0045145", 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"name": "interstrand cross-link repair", "id": "GO:0036297", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0036297", "evidence": [ { "database": "UniProtKB", "id": "P38289", "url": "https://www.uniprot.org/uniprot/P38289" } ], "pmid": "" } ] } ] }, "pro_annotation": [], "site_annotation": [ { "start_pos": 153, "end_pos": 155, "annotation": "n_glycosylation_sequon" }, { "start_pos": 408, "end_pos": 410, "annotation": "n_glycosylation_sequon" }, { "start_pos": 53, "end_pos": 55, "annotation": "n_glycosylation_sequon" }, { "start_pos": 68, "end_pos": 70, "annotation": "n_glycosylation_sequon" } ], "ptm_annotation": [], "interactions": [], "reactions": [], "reaction_participants": [], "reaction_enzymes": [], "biomarkers": [], "synthesized_glycans": [], "cluster_types": [], "section_stats": [ { "table_id": "glycosylation", "table_stats": [ { "field": "total", "count": 0 }, { "field": "total_sites", "count": 0 }, { "field": "n_linked_glycans", "count": 0 }, { "field": 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