{ "uniprot_canonical_ac": "P32047-1", "uniprot_ac": "P32047", "uniprot_id": "MLF3_YEAST", "mass": { "chemical_mass": 49210.0, "monoisotopic_mass": 49210.0 }, "refseq": { "ac": "NP_014325", "name": "Mlf3p", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_014325" }, "gene_names": [ { "name": "MLF3", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/P32047", "id": "P32047" }, { "name": "MLF3", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_014325", "id": "NP_014325" }, { "name": "N2359", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/P32047", "id": "P32047" }, { "name": "YMK1", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_014325", "id": "NP_014325" }, { "name": "YMK1", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/P32047", "id": "P32047" } ], "protein_names": [ { "name": "Protein MLF3", "resource": "UniProtKB", "type": "recommended", "url": "", "id": "P32047" }, { "name": "Mlf3p", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_014325", "id": "NP_014325" }, { "name": "Multicopy suppressor of leflunomide sensitivity 3", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "P32047" } ], "function": [], "disease": [], "snv": [], "mutagenesis": [], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Saccharomyces cerevisiae S288C", "taxid": 559292, "evidence": [ { "database": "UniProtKB", "id": "P32047", "url": "https://www.uniprot.org/uniprot/P32047" } ], "common_name": "Yeast" } ], "gene": [ { "name": "MLF3", "url": "https://www.uniprot.org/uniprot/P32047", "locus": { "chromosome": "XIV", "start_pos": 486766, "end_pos": 488124, "strand": "+", "evidence": [ { "database": "Ensembl Gene", "id": "YNL074C", "url": "https://www.ensembl.org/id/YNL074C" } ] } } ], "crossref": [ { "id": "855650", "url": "https://www.ncbi.nlm.nih.gov/gene/855650", "database": "GeneID", "categories": [ "Genome Annotation" ] }, { "id": "P32047", "url": "https://www.ebi.ac.uk/intact/interactors/id:P32047*", "database": "IntAct", "categories": [ "Other" ] }, { "id": "sce:YNL074C", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+sce:YNL074C", "database": "KEGG Pathway", "categories": [ "Enzyme and Pathway" ] }, { "id": "P32047", "url": "https://oglcnac.org/atlas/detail/P32047", "database": "O-GlcNAc Atlas", "categories": [ "Glycoproteomics" ] }, { "id": "P32047", "url": "https://www.oglcnac.mcw.edu/search/?query_protein=P32047", "database": "O-GlcNAc Database", "categories": [ "Glycoproteomics" ] }, { "id": "NINNTGM", "url": "https://omabrowser.org/oma/group/NINNTGM/", "database": "OMA", "categories": [ "Protein Classification" ] }, { "id": "2053954at2759", "url": "http://www.orthodb.org/?query=2053954at2759", "database": "OrthoDB", "categories": [ "Protein Classification" ] }, { "id": "PR_P32047", "url": "http://purl.obolibrary.org/obo/PR_P32047", "database": "PRO", "categories": [ "Protein" ] }, { "id": "NP_014325", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_014325", "database": "RefSeq", "categories": [ "Protein" ] }, { "id": "S000005018", "url": "https://www.yeastgenome.org/locus/S000005018", "database": "SGD", "categories": [ "Organism-specific" ] }, { "id": "P32047", "url": "https://www.uniprot.org/uniprot/P32047", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "P32047-1", "url": "https://www.uniprot.org/uniprot/P32047-1", "database": "UniProtKB", "categories": [ "Protein" ] } ], "sequence": { "sequence": "MCVYKSNSNNSNPSFIFERTVQEASSNDLFLQPPVSASNTSHSSRSNSFYNLQTISPIPISGSEVRTPSLRKNSNNVSSPLDNVIPTSRSASNSTTSSLAHQEYILNPICNMQNHHHRRRTLENSVAPALDASCSIVNDENTDLSDVDMVYSRRPSSAVSLNMALLARTNSATLPSSESSPASPDLKLSRSHSHSAATRPTLNNINNTGMTTTTSNGEPNSRILRFYSYVDMLNDEKLAQANNTPTSRPPMKSQAYSCPFILKRSPPQAYSSSSATTTFSNPFIKTTELPATSPYVSPQQSARQYSNNANNNAKSPKNRSSSILFQRQSILSNVDPVANMHKNPKFQIESSDSEEEDLTMDMLDPSFPLSSSLRSSANLASNPELATQTPLSTSSSYTAIGKPMPLSTDPSYVSSSNTLSSEHELRVEKVSEVLKKKVSNGGFSTEFNSCDT", "length": 452, "header": "sp|P32047-1|MLF3_YEAST Isoform 1 of Protein MLF3 OS=Saccharomyces cerevisiae S288C OX=559292 GN=MLF3 PE=1 SV=2 CANONICAL=P32047-1" }, "isoforms": [ { "isoform_ac": "P32047-1", "url": "", "sequence": { "sequence": "MCVYKSNSNNSNPSFIFERTVQEASSNDLFLQPPVSASNTSHSSRSNSFYNLQTISPIPISGSEVRTPSLRKNSNNVSSPLDNVIPTSRSASNSTTSSLAHQEYILNPICNMQNHHHRRRTLENSVAPALDASCSIVNDENTDLSDVDMVYSRRPSSAVSLNMALLARTNSATLPSSESSPASPDLKLSRSHSHSAATRPTLNNINNTGMTTTTSNGEPNSRILRFYSYVDMLNDEKLAQANNTPTSRPPMKSQAYSCPFILKRSPPQAYSSSSATTTFSNPFIKTTELPATSPYVSPQQSARQYSNNANNNAKSPKNRSSSILFQRQSILSNVDPVANMHKNPKFQIESSDSEEEDLTMDMLDPSFPLSSSLRSSANLASNPELATQTPLSTSSSYTAIGKPMPLSTDPSYVSSSNTLSSEHELRVEKVSEVLKKKVSNGGFSTEFNSCDT", "length": 452, "header": "sp|P32047-1|MLF3_YEAST Isoform 1 of Protein MLF3 OS=Saccharomyces cerevisiae S288C OX=559292 GN=MLF3 PE=1 SV=2 CANONICAL=P32047-1" }, "locus": { "chromosome": "XIV", "start_pos": 488124, "end_pos": 486769, "strand": "+", "evidence": [ { "database": "Ensembl Transcript", "id": "YNL074C_mRNA", "url": "https://www.ensembl.org/id/YNL074C_mRNA" }, { "database": "Ensembl Peptide", "id": "YNL074C", "url": "https://www.ensembl.org/id/YNL074C" } ] } } ], "glycosylation": [ { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 265, "start_aa": "Ser", "end_pos": 265, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser265", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 272, "start_aa": "Ser", "end_pos": 272, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser272", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 273, "start_aa": "Ser", "end_pos": 273, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser273", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 274, "start_aa": "Ser", "end_pos": 274, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser274", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 280, "start_aa": "Ser", "end_pos": 280, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser280", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "T", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 286, "start_aa": "Thr", "end_pos": 286, "end_aa": "Thr", "residue": "Thr", "site_lbl": "Thr286", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "T", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 287, "start_aa": "Thr", "end_pos": 287, "end_aa": "Thr", "residue": "Thr", "site_lbl": "Thr287", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "T", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 292, "start_aa": "Thr", "end_pos": 292, "end_aa": "Thr", "residue": "Thr", "site_lbl": "Thr292", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 293, "start_aa": "Ser", "end_pos": 293, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser293", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 297, "start_aa": "Ser", "end_pos": 297, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser297", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 301, "start_aa": "Ser", "end_pos": 301, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser301", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 320, "start_aa": "Ser", "end_pos": 320, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser320", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 321, "start_aa": "Ser", "end_pos": 321, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser321", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 322, "start_aa": "Ser", "end_pos": 322, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser322", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 74, "start_aa": "Ser", "end_pos": 74, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser74", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 78, "start_aa": "Ser", "end_pos": 78, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser78", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 79, "start_aa": "Ser", "end_pos": 79, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser79", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "S", "relation": "attached", "comment": "O-GlcNAcylation", "start_pos": 88, "start_aa": "Ser", "end_pos": 88, "end_aa": "Ser", "residue": "Ser", "site_lbl": "Ser88", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Atlas", "url": "https://oglcnac.org/atlas/detail/P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] }, { "glytoucan_ac": "G49108TO", "type": "O-linked", "subtype": "O-GlcNAcylation", "site_category": "reported_with_glycan", "site_seq": "", "relation": "attached", "comment": "O-GlcNAcylation", "evidence": [ { "id": "P32047", "database": "O-GlcNAc Database", "url": "https://www.oglcnac.mcw.edu/search/?query_protein=P32047" }, { "id": "33229814", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33229814" } ] } ], "phosphorylation": [ { "start_pos": 11, "end_pos": 11, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "Phosphoserine", "evidence": [ { "database": "UniProtKB", "id": "P32047", "url": "https://www.uniprot.org/uniprot/P32047" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "iPTMnet", "id": "P32047", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P32047" }, { "database": "PubMed", "id": "19684113", "url": "https://glygen.org/publication/PubMed/19684113" }, { "database": "PubMed", "id": "20702584", "url": "https://glygen.org/publication/PubMed/20702584" }, { "database": "PubMed", "id": "21177495", "url": "https://glygen.org/publication/PubMed/21177495" }, { "database": "SGD", "id": "P32047", "url": "https://www.yeastgenome.org/locus/P32047" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ], "site_lbl": "Ser11" }, { "start_pos": 121, "end_pos": 121, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Thr", "comment": "Phosphothreonine", "evidence": [ { "database": "UniProtKB", "id": "P32047", "url": "https://www.uniprot.org/uniprot/P32047" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "iPTMnet", "id": "P32047", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P32047" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" }, { "database": "SGD", "id": "P32047", "url": "https://www.yeastgenome.org/locus/P32047" } ], "site_lbl": "Thr121" }, { "start_pos": 125, "end_pos": 125, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "iPTMnet", "id": "P32047", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P32047" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "SGD", "id": 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Balakrishnan Rama, Costanzo Maria C, Dwight Selina S, Hitz Benjamin C, Karra Kalpana, Nash Robert S, Weng Shuai, Wong Edith D, Lloyd Paul, Skrzypek Marek S, Miyasato Stuart R, Simison Matt, Cherry J Michael", "evidence": [ { "database": "UniProtKB", "id": "P32047", "url": "https://www.uniprot.org/uniprot/P32047" } ], "reference": [ { "type": "PubMed", "id": "24374639", "url": "https://glygen.org/publication/PubMed/24374639" } ] }, { "title": "Identification of novel O-GlcNAc transferase substrates using yeast cells expressing OGT.", "journal": "The Journal of general and applied microbiology", "date": "2021-04-16", "authors": "Li Feng, Yang Ganglong, Tachikawa Hiroyuki, Shao Kankai, Yang Yan, Gao Xiao-Dong, Nakanishi Hideki", "evidence": [ { "database": "O-GlcNAc Database", "id": "P32047", "url": "https://www.oglcnac.mcw.edu/search/?query_protein=P32047" }, { "database": "O-GlcNAc Atlas", "id": "P32047", "url": "https://oglcnac.org/atlas/detail/P32047" } ], "reference": [ { "type": 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