{ "uniprot_canonical_ac": "P07245-1", "uniprot_ac": "P07245", "uniprot_id": "C1TC_YEAST", "mass": { "chemical_mass": 102205.0, "monoisotopic_mass": 102205.0 }, "refseq": { "ac": "NP_011720", "name": "trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase ADE3", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_011720" }, "gene_names": [ { "name": "ADE3", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/P07245", "id": "P07245" }, { "name": "ADE3", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_011720", "id": "NP_011720" }, { "name": "G7733", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/P07245", "id": "P07245" } ], "protein_names": [ { "name": "C-1-tetrahydrofolate synthase, cytoplasmic", "resource": "UniProtKB", "type": "recommended", "url": "", "id": "P07245" }, { "name": "C1-THF synthase", "resource": "UniProtKB", "type": "synonym", "url": "", "id": "P07245" }, { "name": "trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase ADE3", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_011720", "id": "NP_011720" } ], "function": [ { "annotation": "Cytoplasmic isozyme of C-1-tetrahydrofolate synthase. The trifunctional enzyme catalyzes the interconversion of the one-carbon derivatives of tetrahydrofolate (THF) between different oxidation states by the enzymatic activities 10-formyltetrahydrofolate synthetase, 5,lO-methenyltetrahydrofolate cyclohydrolase, and 5,lO-methylenetetrahydrofolate dehydrogenase. Involved in the generation of one-carbon intermediates in the biosynthesis of the purine bases.", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245#function" }, { "database": "PubMed", "id": "329838", "url": "https://glygen.org/publication/PubMed/329838" }, { "database": "PubMed", "id": "8464869", "url": "https://glygen.org/publication/PubMed/8464869" }, { "database": "PubMed", "id": "3514599", "url": "https://glygen.org/publication/PubMed/3514599" } ] } ], "disease": [ { "disease_id": "DOID:0080016", "recommended_name": { "id": "DOID:0080016", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:0080016", "name": "spina bifida", "description": "A neural tube defect that is characterized by incomplete closing of the spine and membranes around the spinal cord during early development." }, "synonyms": [], "evidence": [ { "id": "0080016", "database": "Alliance of Genome Resources", "url": "https://www.alliancegenome.org/disease/DOID:0080016" } ] }, { "disease_id": "DOID:0080074", "recommended_name": { "id": "DOID:0080074", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:0080074", "name": "neural tube defect", "description": "A physical disorder characterized by incomplete closure of the neural tube." }, "synonyms": [ { "id": "MONDO:0011120", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0011120", "name": "neural tube defects, folate-sensitive", "description": "" }, { "id": "MONDO:0011120", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0011120", "name": "neural tube defects, folate-sensitive", "description": "" }, { "id": "MONDO:0011120", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0011120", "name": "NTD, folate-sensitive", "description": "" }, { "id": "MONDO:0011120", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0011120", "name": "NTDFS", "description": "" }, { "id": "MONDO:0011120", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0011120", "name": "neural tube defects, folate-sensitive, susceptibility to", "description": "" }, { "id": "MONDO:0011120", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0011120", "name": "neural tube defects, susceptibility to", "description": "" }, { "id": "MONDO:0011120", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0011120", "name": "spina bifida, folate-sensitive", "description": "" }, { "id": "MONDO:0010525", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010525", "name": "neural tube defects, X-linked", "description": "" }, { "id": "MONDO:0010525", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010525", "name": "neural tube defects, X-linked", "description": "" }, { "id": "MONDO:0010525", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010525", "name": "X-linked anencephaly/spina bifida", "description": "" }, { "id": "MONDO:0010525", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010525", "name": "anencephaly and spina bifida X-linked", "description": "" }, { "id": "MONDO:0010525", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0010525", "name": "spina bifida, X-linked", "description": "" }, { "id": "MIM:601634", "resource": "MIM", "url": "https://www.omim.org/entry/601634", "name": "Neural tube defects, folate-sensitive", "description": "Neural tube defects, folate-sensitive" } ], "evidence": [ { "id": "0080074", "database": "Alliance of Genome Resources", "url": "https://www.alliancegenome.org/disease/DOID:0080074" } ] }, { "disease_id": "DOID:0080633", "recommended_name": { "id": "DOID:0080633", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:0080633", "name": "developmental cardiac valvular defect", "description": "A physical disorder that has_material_basis_in homozygous or compound heterozygous mutation in the PLD1 gene on chromosome 3q26." }, "synonyms": [ { "id": "MONDO:0008913", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008913", "name": "cardiac valvular defect, developmental", "description": "" }, { "id": "MONDO:0008913", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008913", "name": "cardiac valvular defect, developmental", "description": "" }, { "id": "MONDO:0008913", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008913", "name": "CARDIAC valvular defect, developmental", "description": "" }, { "id": "MONDO:0008913", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008913", "name": "CVDD", "description": "" }, { "id": "MIM:212093", "resource": "MIM", "url": "https://www.omim.org/entry/212093", "name": "Cardiac valvular dysplasia 1", "description": "Cardiac valvular dysplasia 1" } ], "evidence": [ { "id": "0080633", "database": "Alliance of Genome Resources", "url": "https://www.alliancegenome.org/disease/DOID:0080633" } ] }, { "disease_id": "DOID:14250", "recommended_name": { "id": "DOID:14250", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:14250", "name": "Down syndrome", "description": "A chromosomal disease that is characterized by flat-looking facial features and weak muscle tone (hypotonia) in infancy and is caused by trisomy of all or a critical portion of chromosome 21 and is associated with intellectual disability." }, "synonyms": [ { "id": "DOID:14250", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:14250", "name": "Complete trisomy 21 syndrome", "description": "" }, { "id": "DOID:14250", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:14250", "name": "Down's syndrome", "description": "" }, { "id": "DOID:14250", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:14250", "name": "Down's syndrome - trisomy 21", "description": "" }, { "id": "DOID:14250", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:14250", "name": "Downs syndrome", "description": "" }, { "id": "DOID:14250", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:14250", "name": "G Trisomy", "description": "" }, { "id": "DOID:14250", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:14250", "name": "trisomy 21 syndrome", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "Down syndrome", "description": "Down syndrome is a chromosomal abnormality caused by the presence of a third (partial or total) copy of the chromosome 21 genetic material and that is characterized by variable intellectual disability, muscular hypotonia, and joint laxity, often associated with a characteristic facial dysmorphism and various anomalies such as cardiac, gastrointestinal, or endocrine defects." }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "Down syndrome", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "Down syndrome, Isolated cases", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "Down's syndrome", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "Down's syndrome - trisomy 21", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "Downs syndrome", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "G trisomy", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "leukemia, megakaryoblastic, with or without Down syndrome, somatic", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "trisomy 21 (Down syndrome)", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "complete trisomy 21 syndrome", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "trisomy 21", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "trisomy 21 syndrome", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "Down syndrome chromosome region", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "Down syndrome critical region", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "leukemia, megakaryoblastic, of Down syndrome", "description": "" }, { "id": "MONDO:0008608", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008608", "name": "transient myeloproliferative disorder of Down syndrome", "description": "" } ], "evidence": [ { "id": "14250", "database": "Alliance of Genome Resources", "url": "https://www.alliancegenome.org/disease/DOID:14250" } ] }, { "disease_id": "DOID:6419", "recommended_name": { "id": "DOID:6419", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:6419", "name": "tetralogy of Fallot", "description": "" }, "synonyms": [ { "id": "DOID:6419", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:6419", "name": "Ventricular septal defect with pulmonary stenosis or atresia, dextraposition of aorta, and hypertrophy of right ventricle", "description": "" }, { "id": "MONDO:0008542", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008542", "name": "tetralogy of fallot", "description": "Tetralogy of Fallot is a congenital cardiac malformation that consists of an interventricular communication, also known as a ventricular septal defect, obstruction of the right ventricular outflow tract, override of the ventricular septum by the aortic root, and right ventricular hypertrophy." }, { "id": "MONDO:0008542", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008542", "name": "tetralogy of fallot", "description": "" }, { "id": "MONDO:0008542", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008542", "name": "ventricular septal defect with pulmonary stenosis or atresia, dextraposition of aorta, and hypertrophy of right ventricle", "description": "" }, { "id": "MONDO:0008542", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008542", "name": "Fallot tetralogy", "description": "" }, { "id": "MONDO:0008542", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008542", "name": "TOF", "description": "" }, { "id": "MONDO:0008542", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0008542", "name": "tetralogy of FALLOT", "description": "" }, { "id": "MIM:187500", "resource": "MIM", "url": "https://www.omim.org/entry/187500", "name": "Tetralogy of Fallot", "description": "Tetralogy of Fallot" } ], "evidence": [ { "id": "6419", "database": "Alliance of Genome Resources", "url": "https://www.alliancegenome.org/disease/DOID:6419" } ] }, { "disease_id": "DOID:674", "recommended_name": { "id": "DOID:674", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:674", "name": "cleft palate", "description": "An orofacial cleft characterized by a fissure of the soft and/or hard palate, due to faulty fusion of the two plates of the skull that form the hard palate." }, "synonyms": [ { "id": "DOID:674", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:674", "name": "Palatoschisis", "description": "" } ], "evidence": [ { "id": "674", "database": "Alliance of Genome Resources", "url": "https://www.alliancegenome.org/disease/DOID:674" } ] }, { "disease_id": "DOID:9296", "recommended_name": { "id": "DOID:9296", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:9296", "name": "cleft lip", "description": "An orofacial cleft that is characterized by a congenital defect in the upper lip where the maxillary prominence fails to merge with the merged medial nasal prominences." }, "synonyms": [ { "id": "DOID:9296", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:9296", "name": "Labium leporinum", "description": "" }, { "id": "DOID:9296", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:9296", "name": "cheiloschisis", "description": "" }, { "id": "DOID:9296", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:9296", "name": "cleft lip, unilateral, complete", "description": "" }, { "id": "DOID:9296", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:9296", "name": "complete unilateral cleft lip", "description": "" }, { "id": "DOID:9296", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:9296", "name": "hare lip", "description": "" } ], "evidence": [ { "id": "9296", "database": "Alliance of Genome Resources", "url": "https://www.alliancegenome.org/disease/DOID:9296" } ] } ], "snv": [], "mutagenesis": [ { "start_pos": 11, "end_pos": 11, "sequence_org": "C", "sequence_mut": "S", "comment": "Almost no effect on activity.", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" }, { "database": "PubMed", "id": "2541774", "url": "https://glygen.org/publication/PubMed/2541774" } ] }, { "start_pos": 144, "end_pos": 144, "sequence_org": "C", "sequence_mut": "S", "comment": "Cyclohydrolase activity is reduced 20-fold. Dehydrogenase Km is increased 7-fold.", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" }, { "database": "PubMed", "id": "2541774", "url": "https://glygen.org/publication/PubMed/2541774" } ] }, { "start_pos": 257, "end_pos": 257, "sequence_org": "C", "sequence_mut": "S", "comment": "Cyclohydrolase activity is increased 2-fold. Dehydrogenase Km is increased 2-fold.", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" }, { "database": "PubMed", "id": "2541774", "url": "https://glygen.org/publication/PubMed/2541774" } ] } ], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Saccharomyces cerevisiae S288C", "taxid": 559292, "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" } ], "common_name": "Yeast" } ], "gene": [ { "name": "ADE3", "url": "https://www.uniprot.org/uniprot/P07245", "locus": {} } ], "crossref": [ { "id": "3.5.4.9", "url": "https://www.brenda-enzymes.org/enzyme.php?ecno=3.5.4.9&UniProtAcc=P07245", "database": "BRENDA", "categories": [ "Enzyme and Pathway" ] }, { "id": "6.3.4.3", "url": "https://www.brenda-enzymes.org/enzyme.php?ecno=6.3.4.3&UniProtAcc=P07245", "database": "BRENDA", "categories": [ "Enzyme and Pathway" ] }, { "id": "cd00477", "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00477", "database": "CDD", "categories": [ "Protein Classification" ] }, { "id": "cd01080", "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd01080", "database": "CDD", "categories": [ "Protein Classification" ] }, { "id": "853118", "url": "https://www.ncbi.nlm.nih.gov/gene/853118", "database": "GeneID", "categories": [ "Genome Annotation" ] }, { "id": "P07245", "url": "https://www.ebi.ac.uk/intact/interactors/id:P07245*", "database": "IntAct", "categories": [ "Other" ] }, { "id": "IPR000559", "url": "https://www.ebi.ac.uk/interpro/entry/IPR000559", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR000672", "url": "https://www.ebi.ac.uk/interpro/entry/IPR000672", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR020628", "url": "https://www.ebi.ac.uk/interpro/entry/IPR020628", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR020630", "url": "https://www.ebi.ac.uk/interpro/entry/IPR020630", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR020631", "url": "https://www.ebi.ac.uk/interpro/entry/IPR020631", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR020867", "url": "https://www.ebi.ac.uk/interpro/entry/IPR020867", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR027417", "url": "https://www.ebi.ac.uk/interpro/entry/IPR027417", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR036291", "url": "https://www.ebi.ac.uk/interpro/entry/IPR036291", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "IPR046346", "url": "https://www.ebi.ac.uk/interpro/entry/IPR046346", "database": "InterPro", "categories": [ "Protein Classification" ] }, { "id": "sce:YGR204W", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+sce:YGR204W", "database": "KEGG Pathway", "categories": [ "Enzyme and Pathway" ] }, { "id": "CKQIANI", "url": "https://omabrowser.org/oma/group/CKQIANI/", "database": "OMA", "categories": [ "Protein Classification" ] }, { "id": "651667at2759", "url": "http://www.orthodb.org/?query=651667at2759", "database": "OrthoDB", "categories": [ "Protein Classification" ] }, { "id": "PTHR48099", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR48099", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PTHR48099:SF8", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR48099:SF8", "database": "PANTHER", "categories": [ "Protein Classification" ] }, { "id": "PF00763", "url": "http://pfam.xfam.org/family/PF00763", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PF01268", "url": "http://pfam.xfam.org/family/PF01268", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PF02882", "url": "http://pfam.xfam.org/family/PF02882", "database": "Pfam", "categories": [ "Protein Classification" ] }, { "id": "PR_P07245", "url": "http://purl.obolibrary.org/obo/PR_P07245", "database": "PRO", "categories": [ "Protein" ] }, { "id": "NP_011720", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_011720", "database": "RefSeq", "categories": [ "Protein" ] }, { "id": "S000003436", "url": "https://www.yeastgenome.org/locus/S000003436", "database": "SGD", "categories": [ "Organism-specific" ] }, { "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245", "database": "UniProtKB", "categories": [ "Protein" ] }, { "id": "P07245-1", "url": "https://www.uniprot.org/uniprot/P07245-1", "database": "UniProtKB", "categories": [ "Protein" ] } ], "sequence": { "sequence": "MAGQVLDGKACAQQFRSNIANEIKSIQGHVPGFAPNLAIIQVGNRPDSATYVRMKRKAAEEAGIVANFIHLDESATEFEVLRYVDQLNEDPHTHGIIVQLPLPAHLDEDRITSRVLAEKDVDGFGPTNIGELNKKNGHPFFLPCTPKGIIELLHKANVTIEGSRSVVIGRSDIVGSPVAELLKSLNSTVTITHSKTRDIASYLHDADIVVVAIGQPEFVKGEWFKPRDGTSSDKKTVVIDVGTNYVADPSKKSGFKCVGDVEFNEAIKYVHLITPVPGGVGPMTVAMLMQNTLIAAKRQMEESSKPLQIPPLPLKLLTPVPSDIDISRAQQPKLINQLAQELGIYSHELELYGHYKAKISPKVIERLQTRQNGKYILVSGITPTPLGEGKSTTTMGLVQALTAHLGKPAIANVRQPSLGPTLGVKGGAAGGGYSQVIPMDEFNLHLTGDIHAIGAANNLLAAAIDTRMFHETTQKNDATFYNRLVPRKNGKRKFTPSMQRRLNRLGIQKTNPDDLTPEEINKFARLNIDPDTITIKRVVDINDRMLRQITIGQAPTEKNHTRVTGFDITVASELMAILALSKDLRDMKERIGRVVVAADVNRSPVTVEDVGCTGALTALLRDAIKPNLMQTLEGTPVLVHAGPFANISIGASSVIADRVALKLVGTEPEAKTEAGYVVTEAGFDFTMGGERFFNIKCRSSGLTPNAVVLVATVRALKSHGGAPDVKPGQPLPSAYTEENIEFVEKGAANMCKQIANIKQFGVPVVVAINKFETDTEGEIAAIRKAALEAGAFEAVTSNHWAEGGKGAIDLAKAVIEASNQPVDFHFLYDVNSSVEDKLTTIVQKMYGGAAIDILPEAQRKIDMYKEQGFGNLPICIAKTQYSLSHDATLKGVPTGFTFPIRDVRLSNGAGYLYALAAEIQTIPGLATYAGYMAVEVDDDGEIDGLF", "length": 946, "header": "sp|P07245-1|C1TC_YEAST Isoform 1 of C-1-tetrahydrofolate synthase, cytoplasmic OS=Saccharomyces cerevisiae S288C OX=559292 GN=ADE3 PE=1 SV=1 CANONICAL=P07245-1" }, "isoforms": [ { "isoform_ac": "P07245-1", "url": "", "sequence": { "sequence": 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Isoform 1 of C-1-tetrahydrofolate synthase, cytoplasmic OS=Saccharomyces cerevisiae S288C OX=559292 GN=ADE3 PE=1 SV=1 CANONICAL=P07245-1" }, "locus": { "chromosome": "VII", "start_pos": 908771, "end_pos": 905934, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "YGR204W_mRNA", "url": "https://www.ensembl.org/id/YGR204W_mRNA" }, { "database": "Ensembl Peptide", "id": "YGR204W", "url": "https://www.ensembl.org/id/YGR204W" } ] } } ], "glycosylation": [], "phosphorylation": [ { "start_pos": 176, "end_pos": 176, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "Phosphoserine", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "iPTMnet", "id": "P07245", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P07245" }, { "database": "SGD", "id": "P07245", "url": "https://www.yeastgenome.org/locus/P07245" } ], "site_lbl": "Ser176" }, { "start_pos": 318, "end_pos": 318, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Thr", "comment": "Phosphothreonine", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "iPTMnet", "id": "P07245", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P07245" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" }, { "database": "PubMed", "id": "20702584", "url": "https://glygen.org/publication/PubMed/20702584" }, { "database": "PubMed", "id": "21126336", "url": "https://glygen.org/publication/PubMed/21126336" }, { "database": "SGD", "id": "P07245", "url": "https://www.yeastgenome.org/locus/P07245" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ], "site_lbl": "Thr318" }, { "start_pos": 322, "end_pos": 322, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "Phosphoserine", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" }, { "database": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" }, { "database": "iPTMnet", "id": "P07245", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P07245" }, { "database": "PubMed", "id": "17330950", "url": "https://glygen.org/publication/PubMed/17330950" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" }, { "database": "SGD", "id": "P07245", "url": "https://www.yeastgenome.org/locus/P07245" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ], "site_lbl": "Ser322" }, { "start_pos": 384, "end_pos": 384, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Thr", "comment": "", "evidence": [ { "database": "iPTMnet", "id": "P07245", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P07245" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" }, { "database": "SGD", "id": "P07245", "url": "https://www.yeastgenome.org/locus/P07245" } ], "site_lbl": "Thr384" }, { "start_pos": 510, "end_pos": 510, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Thr", "comment": "", "evidence": [ { "database": "SGD", "id": "P07245", "url": "https://www.yeastgenome.org/locus/P07245" }, { "database": "iPTMnet", "id": "P07245", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P07245" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ], "site_lbl": "Thr510" }, { "start_pos": 884, "end_pos": 884, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "", "evidence": [ { "database": "SGD", "id": "P07245", "url": "https://www.yeastgenome.org/locus/P07245" }, { "database": "iPTMnet", "id": "P07245", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P07245" }, { "database": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ], "site_lbl": "Ser884" }, { "start_pos": 894, "end_pos": 894, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Thr", "comment": "", "evidence": [ { "database": "iPTMnet", "id": "P07245", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P07245" }, { "database": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" }, { "database": "SGD", "id": "P07245", "url": "https://www.yeastgenome.org/locus/P07245" } ], "site_lbl": "Thr894" } ], "glycation": [], "keywords": [ "enzyme" ], "publication": [ { "title": "Global analysis of protein localization in budding yeast.", "journal": "Nature", "date": "2003", "authors": "Weissman J.S., Carroll A.S., Howson R.W., Huh W.-K., O'Shea E.K., Falvo J.V., Gerke L.C.", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" } ], "reference": [ { "type": "PubMed", "id": "14562095", "url": "https://glygen.org/publication/PubMed/14562095" } ] }, { "title": "Global analysis of protein expression in yeast.", "journal": "Nature", "date": "2003", "authors": "Dephoure N., Weissman J.S., Howson R.W., Huh W.-K., O'Shea E.K., Belle A., Bower K., Ghaemmaghami S.", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" } ], "reference": [ { "type": "PubMed", "id": "14562106", "url": "https://glygen.org/publication/PubMed/14562106" } ] }, { "title": "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.", "journal": "Genome Res.", "date": "2007", "authors": "Hu Y., LaBaer J., Moreira D., Raphael J., Rolfs A., Zuo D., Richardson A., Simpson A.J.G., Zhu C., Harlow E., Camargo A.A., Jepson D., Kelley F., McCarron S., Taycher E., Kolodner R.D., Kane M.F., Williamson J., Berger M.F., Bulyk M.L., Bhullar B., Mohr S., Marsischky G., Murthy T.V.S.", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" } ], "reference": [ { "type": "PubMed", "id": "17322287", "url": "https://glygen.org/publication/PubMed/17322287" } ] }, { "title": "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.", "journal": "J. Proteome Res.", "date": "2007", "authors": "Li Xue, Gerber Scott A, Rudner Adam D, Beausoleil Sean A, Haas Wilhelm, Vill\u00e9n Judit, Elias Joshua E, Gygi Steve P", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" }, { "database": "iPTMnet", "id": "P07245", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P07245" } ], "reference": [ { "type": "PubMed", "id": "17330950", "url": "https://glygen.org/publication/PubMed/17330950" } ] }, { "title": "A multidimensional chromatography technology for in-depth phosphoproteome analysis.", "journal": "Molecular & cellular proteomics : MCP", "date": "2008-07", "authors": "Albuquerque Claudio P, Smolka Marcus B, Payne Samuel H, Bafna Vineet, Eng Jimmy, Zhou Huilin", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" }, { "database": "iPTMnet", "id": "P07245", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P07245" } ], "reference": [ { "type": "PubMed", "id": "18407956", "url": "https://glygen.org/publication/PubMed/18407956" } ] }, { "title": "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.", "journal": "Science (New York, N.Y.)", "date": "2009-09-25", "authors": "Holt Liam J, Tuch Brian B, Vill\u00e9n Judit, Johnson Alexander D, Gygi Steven P, Morgan David O", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" }, { "database": "iPTMnet", "id": "P07245", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P07245" } ], "reference": [ { "type": "PubMed", "id": "19779198", "url": "https://glygen.org/publication/PubMed/19779198" } ] }, { "title": "The rapamycin-sensitive phosphoproteome reveals that TOR controls protein kinase A toward some but not all substrates.", "journal": "Molecular biology of the cell", "date": "2010-10-01", "authors": "Soulard Alexandre, Cremonesi Alessio, Moes Suzette, Sch\u00fctz Fr\u00e9d\u00e9ric, Jen\u00f6 Paul, Hall Michael N", "evidence": [ { "database": "iPTMnet", "id": "P07245", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P07245" } ], "reference": [ { "type": "PubMed", "id": "20702584", "url": "https://glygen.org/publication/PubMed/20702584" } ] }, { "title": "Perturbation of the yeast N-acetyltransferase NatB induces elevation of protein phosphorylation levels.", "journal": "BMC genomics", "date": "2010-12-02", "authors": "Helbig Andreas O, Rosati Sara, Pijnappel Pim W W M, van Breukelen Bas, Timmers Marc H T H, Mohammed Shabaz, Slijper Monique, Heck Albert J R", "evidence": [ { "database": "iPTMnet", "id": "P07245", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P07245" } ], "reference": [ { "type": "PubMed", "id": "21126336", "url": "https://glygen.org/publication/PubMed/21126336" } ] }, { "title": "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "date": "2012", "authors": "Van Damme P, Lasa M, Polevoda B, Gazquez C, Elosegui-Artola A, Kim DS, De Juan-Pardo E, Demeyer K, Hole K, Larrea E, Timmerman E, Prieto J, Arnesen T, Sherman F, Gevaert K, Aldabe R", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" } ], "reference": [ { "type": "PubMed", "id": "22814378", "url": "https://glygen.org/publication/PubMed/22814378" } ] }, { "title": "Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.", "journal": "Nature methods", "date": "2013-07", "authors": "Swaney Danielle L, Beltrao Pedro, Starita Lea, Guo Ailan, Rush John, Fields Stanley, Krogan Nevan J, Vill\u00e9n Judit", "evidence": [ { "database": "iPTMnet", "id": "P07245", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P07245" } ], "reference": [ { "type": "PubMed", "id": "23749301", "url": "https://glygen.org/publication/PubMed/23749301" } ] }, { "title": "The reference genome sequence of Saccharomyces cerevisiae: Then and now.", "journal": "G3 (Bethesda)", "date": "2014", "authors": "Engel Stacia R, Dietrich Fred S, Fisk Dianna G, Binkley Gail, Balakrishnan Rama, Costanzo Maria C, Dwight Selina S, Hitz Benjamin C, Karra Kalpana, Nash Robert S, Weng Shuai, Wong Edith D, Lloyd Paul, Skrzypek Marek S, Miyasato Stuart R, Simison Matt, Cherry J Michael", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" } ], "reference": [ { "type": "PubMed", "id": "24374639", "url": "https://glygen.org/publication/PubMed/24374639" } ] }, { "title": "Site-directed mutagenesis of yeast C1-tetrahydrofolate synthase: analysis of an overlapping active site in a multifunctional enzyme.", "journal": "Biochemistry", "date": "1989", "authors": "Barlowe C K, Williams M E, Rabinowitz J C, Appling D R", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" } ], "reference": [ { "type": "PubMed", "id": "2541774", "url": "https://glygen.org/publication/PubMed/2541774" } ] }, { "title": "Formyl-methenyl-methylenetetrahydrofolate synthetase (combined); correlation of enzymic activities with limited proteolytic degradation of the protein from yeast.", "journal": "Biochem. Biophys. Res. Commun.", "date": "1977", "authors": "Williams G.R., Paukert J.L., Rabinowitz J.C.", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" } ], "reference": [ { "type": "PubMed", "id": "329838", "url": "https://glygen.org/publication/PubMed/329838" } ] }, { "title": "Nucleotide sequence of the Saccharomyces cerevisiae ADE3 gene encoding C1-tetrahydrofolate synthase.", "journal": "J. Biol. Chem.", "date": "1986", "authors": "Rabinowitz J.C., Staben C.", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" } ], "reference": [ { "type": "PubMed", "id": "3514599", "url": "https://glygen.org/publication/PubMed/3514599" } ] }, { "title": "Function of yeast cytoplasmic C1-tetrahydrofolate synthase.", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "date": "1993", "authors": "Song J.M., Rabinowitz J.C.", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" } ], "reference": [ { "type": "PubMed", "id": "8464869", "url": "https://glygen.org/publication/PubMed/8464869" } ] }, { "title": "Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs.", "journal": "Yeast", "date": "1996", "authors": "Maia e Silva A., Soares H., Rodrigues-Pousada C., Guerreiro P., Barreiros T., Cyrne L.", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" } ], "reference": [ { "type": "PubMed", "id": "8904340", "url": "https://glygen.org/publication/PubMed/8904340" } ] }, { "title": "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.", "journal": "Nature", "date": "1997", "authors": "Mewes H.-W., Minenkova O., Schaefer M., Scharfe M., Wambutt R., Wedler H., Verhasselt P., Hansen M., Rose M., Durand P., Brueckner M., Plevani P., Netter P., Rieger M., Zimmermann M., Portetelle D., Wolf K., Bertani I., Delius H., Kleine K., Portillo F., Hofmann B., Albers M., Schaefer B., Kramer W., Castagnoli L., Volckaert G., Koetter P., Entian K.-D., Kramer B., Rinaldi T., Thierry A., Louis E.J., Vandenbol M., Martegani E., Robben J., Nombela C., Vanoni M., Arroyo J., Eraso P., Mazon M.J., James C.M., Carignani G., Dujon B., Feuermann M., Goffeau A., Hani J., Hilger F., Klima R., Mueller-Auer S., Oliver S.G., Potier S., Rodrigues-Pousada C., Schmidheini T., Souciet J.-L., Steensma H.Y., Zollner A., Bruschi C.V., Escribano V., Frontali L., Guerreiro P., Mazzoni C., Purnelle B., Rodriguez-Torres A.M., Skala J., Talla E., Voet M., de Wergifosse P., Agostoni-Carbone M.L., Albermann K., Backes U., Barreiros T., Bjourson A.J., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delneri D., Fabiani L., Fartmann B., Feroli F., Garcia-Gonzalez M., Garcia-Saez M.I., Hebling U., Hernandez K., Heumann K., Indge K.J., Lauquin G., Leuther H., Maillier E., Marconi A., McReynolds A.D.K., Melchioretto P., Nawrocki A., Neu R., Panzeri L., Paoluzi S., Riles L., Rodriguez-Belmonte E., Ruzzi M., Saliola M., Sanchez-Perez M., Schreer A., Tettelin H., Van Dyck L., Watson M.D., Weber N., Wedler E., Wipfli P., Wright L.F., Zaccaria P., van der Aart Q.J.M.", "evidence": [ { "database": "UniProtKB", "id": "P07245", "url": "https://www.uniprot.org/uniprot/P07245" } ], "reference": [ { "type": "PubMed", "id": "9169869", "url": "https://glygen.org/publication/PubMed/9169869" } ] } ], "pathway": [ { "id": "sce:YGR204W", "name": "", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+sce:YGR204W", "resource": "KEGG Pathway" } ], 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