{
    "uniprot_canonical_ac": "D3ZYQ9-1",
    "uniprot_ac": "D3ZYQ9",
    "uniprot_id": "D3ZYQ9_RAT",
    "mass": {
        "chemical_mass": 113460.0,
        "monoisotopic_mass": 113460.0
    },
    "refseq": {
        "ac": "NP_001101399",
        "name": "E3 ubiquitin-protein ligase BRE1A",
        "summary": "",
        "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001101399"
    },
    "gene_names": [
        {
            "name": "Rnf20",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "https://www.uniprot.org/uniprot/D3ZYQ9",
            "id": "D3ZYQ9"
        },
        {
            "name": "Rnf20",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001101399",
            "id": "NP_001101399"
        }
    ],
    "protein_names": [
        {
            "name": "E3 ubiquitin protein ligase",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "",
            "id": "D3ZYQ9"
        },
        {
            "name": "2.3.2.27",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "",
            "id": "D3ZYQ9"
        },
        {
            "name": "E3 ubiquitin-protein ligase BRE1A",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001101399",
            "id": "NP_001101399"
        },
        {
            "name": "ring finger protein 20, E3 ubiquitin protein ligase",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001101399",
            "id": "NP_001101399"
        }
    ],
    "function": [
        {
            "annotation": "RNF20-drive H2B monoubiquitination, which facilitates phosphorylated mGluR5 transcription in the dorsal horn in the development of neuropathic allodynia.",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001101399",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001101399"
                },
                {
                    "database": "PubMed",
                    "id": "30201771",
                    "url": "https://glygen.org/publication/PubMed/30201771"
                }
            ]
        }
    ],
    "disease": [
        {
            "disease_id": "DOID:1612",
            "recommended_name": {
                "id": "DOID:1612",
                "resource": "Disease Ontology",
                "url": "http://disease-ontology.org/term/DOID:1612",
                "name": "breast cancer",
                "description": "A thoracic cancer that originates in the mammary gland."
            },
            "synonyms": [
                {
                    "id": "DOID:1612",
                    "resource": "Disease Ontology",
                    "url": "http://disease-ontology.org/term/DOID:1612",
                    "name": "breast tumor",
                    "description": ""
                },
                {
                    "id": "DOID:1612",
                    "resource": "Disease Ontology",
                    "url": "http://disease-ontology.org/term/DOID:1612",
                    "name": "malignant neoplasm of breast",
                    "description": ""
                },
                {
                    "id": "DOID:1612",
                    "resource": "Disease Ontology",
                    "url": "http://disease-ontology.org/term/DOID:1612",
                    "name": "malignant tumor of the breast",
                    "description": ""
                },
                {
                    "id": "DOID:1612",
                    "resource": "Disease Ontology",
                    "url": "http://disease-ontology.org/term/DOID:1612",
                    "name": "mammary cancer",
                    "description": ""
                },
                {
                    "id": "DOID:1612",
                    "resource": "Disease Ontology",
                    "url": "http://disease-ontology.org/term/DOID:1612",
                    "name": "mammary tumor",
                    "description": ""
                },
                {
                    "id": "DOID:1612",
                    "resource": "Disease Ontology",
                    "url": "http://disease-ontology.org/term/DOID:1612",
                    "name": "primary breast cancer",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "hereditary breast carcinoma",
                    "description": "Breast carcinoma that has developed in relatives of patients with history of breast carcinoma."
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "breast cancer susceptibility, autosomal dominant, somatic mutation",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "breast cancer, early-onset, susceptibility to, autosomal dominant, somatic mutation",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "breast cancer, invasive ductal, autosomal dominant, somatic mutation",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "breast cancer, lobular, somatic",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "breast cancer, male, susceptibility to, autosomal dominant, somatic mutation",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "breast cancer, protection against, autosomal dominant, somatic mutation",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "breast cancer, somatic",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "breast cancer, susceptibility to, autosomal dominant, somatic mutation",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "familial breast cancer",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "familial breast carcinoma",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "familial cancer of breast",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "familial cancer of the breast",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "hereditary breast cancer",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "hereditary breast carcinoma",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "breast cancer, familial",
                    "description": ""
                },
                {
                    "id": "MONDO:0016419",
                    "resource": "MONDO",
                    "url": "https://monarchinitiative.org/disease/MONDO:0016419",
                    "name": "breast cancer, familial Male",
                    "description": ""
                },
                {
                    "id": "MIM:114480",
                    "resource": "MIM",
                    "url": "https://www.omim.org/entry/114480",
                    "name": "Breast cancer",
                    "description": "Breast cancer"
                }
            ],
            "evidence": [
                {
                    "id": "1612",
                    "database": "Alliance of Genome Resources",
                    "url": "https://www.alliancegenome.org/disease/DOID:1612"
                }
            ]
        },
        {
            "disease_id": "DOID:1682",
            "recommended_name": {
                "id": "DOID:1682",
                "resource": "Disease Ontology",
                "url": "http://disease-ontology.org/term/DOID:1682",
                "name": "congenital heart disease",
                "description": ""
            },
            "synonyms": [
                {
                    "id": "DOID:1682",
                    "resource": "Disease Ontology",
                    "url": "http://disease-ontology.org/term/DOID:1682",
                    "name": "Congenital Heart Defects",
                    "description": ""
                },
                {
                    "id": "DOID:1682",
                    "resource": "Disease Ontology",
                    "url": "http://disease-ontology.org/term/DOID:1682",
                    "name": "Congenital anomaly of heart",
                    "description": ""
                },
                {
                    "id": "DOID:1682",
                    "resource": "Disease Ontology",
                    "url": "http://disease-ontology.org/term/DOID:1682",
                    "name": "Heart Malformation",
                    "description": ""
                },
                {
                    "id": "DOID:1682",
                    "resource": "Disease Ontology",
                    "url": "http://disease-ontology.org/term/DOID:1682",
                    "name": "congenital heart defect",
                    "description": ""
                },
                {
                    "id": "DOID:1682",
                    "resource": "Disease Ontology",
                    "url": "http://disease-ontology.org/term/DOID:1682",
                    "name": "heart defect",
                    "description": ""
                }
            ],
            "evidence": [
                {
                    "id": "1682",
                    "database": "Alliance of Genome Resources",
                    "url": "https://www.alliancegenome.org/disease/DOID:1682"
                }
            ]
        }
    ],
    "snv": [],
    "mutagenesis": [],
    "expression_tissue": [],
    "expression_disease": [],
    "species": [
        {
            "name": "Rattus norvegicus",
            "taxid": 10116,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "D3ZYQ9",
                    "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                }
            ],
            "common_name": "Rat"
        }
    ],
    "gene": [
        {
            "name": "Rnf20",
            "url": "https://www.uniprot.org/uniprot/D3ZYQ9",
            "locus": {
                "chromosome": "5",
                "start_pos": 64001754,
                "end_pos": 63976045,
                "strand": "-",
                "evidence": [
                    {
                        "database": "Ensembl Gene",
                        "id": "ENSRNOG00000006087",
                        "url": "https://www.ensembl.org/id/ENSRNOG00000006087"
                    }
                ]
            }
        }
    ],
    "crossref": [
        {
            "id": "ENSRNOG00000006087",
            "url": "https://bgee.org/?page=gene&gene_id=ENSRNOG00000006087",
            "database": "Bgee",
            "categories": [
                "Gene Expression"
            ]
        },
        {
            "id": "cd16814",
            "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd16814",
            "database": "CDD",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "313216",
            "url": "https://www.ncbi.nlm.nih.gov/gene/313216",
            "database": "GeneID",
            "categories": [
                "Genome Annotation"
            ]
        },
        {
            "id": "IPR001841",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR001841",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR013083",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR013083",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR013956",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR013956",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR017907",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR017907",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR018957",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR018957",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "rno:313216",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+rno:313216",
            "database": "KEGG Pathway",
            "categories": [
                "Enzyme and Pathway"
            ]
        },
        {
            "id": "CEQITRD",
            "url": "https://omabrowser.org/oma/group/CEQITRD/",
            "database": "OMA",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "53681at2759",
            "url": "http://www.orthodb.org/?query=53681at2759",
            "database": "OrthoDB",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR23163",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR23163",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR23163:SF2",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR23163:SF2",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PF00097",
            "url": "http://pfam.xfam.org/family/PF00097",
            "database": "Pfam",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "R-RNO-8866654",
            "url": "https://www.reactome.org/PathwayBrowser/#R-RNO-8866654",
            "database": "Reactome",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "R-RNO-9013422",
            "url": "https://www.reactome.org/PathwayBrowser/#R-RNO-9013422",
            "database": "Reactome",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "NP_001101399",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001101399",
            "database": "RefSeq",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "1311936",
            "url": "http://rgd.mcw.edu/tools/genes/genes_view.cgi?id=1311936",
            "database": "RGD",
            "categories": [
                "Organism-specific"
            ]
        },
        {
            "id": "A0A8I6A812",
            "url": "https://www.uniprot.org/uniprot/A0A8I6A812",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "A0A8I6A812-1",
            "url": "https://www.uniprot.org/uniprot/A0A8I6A812-1",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "A0A8I6AJL7",
            "url": "https://www.uniprot.org/uniprot/A0A8I6AJL7",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "A0A8I6AJL7-1",
            "url": "https://www.uniprot.org/uniprot/A0A8I6AJL7-1",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "D3ZYQ9",
            "url": "https://www.uniprot.org/uniprot/D3ZYQ9",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "D3ZYQ9-1",
            "url": "https://www.uniprot.org/uniprot/D3ZYQ9-1",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        }
    ],
    "sequence": {
        "sequence": "MSGIGNKRAAGEPGTSVPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQSKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLDQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVDLLSRKLNSGDNLIMEEAVQELNSFLAQENVRLQELTDLLQEKHHTMSQEFCKLQGKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDFEEVTTQNEKLKVELRSAVEEVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDAKDEPTELKQDSEDLATHSSALKASQEDEVKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDPVKDKDKGKHDDGRKKEAEIIKQLKTELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAVAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDLFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG",
        "length": 973,
        "header": "tr|D3ZYQ9-1|D3ZYQ9_RAT Isoform 1 of E3 ubiquitin protein ligase  OS=Rattus norvegicus OX=10116 GN=Rnf20 PE=1 SV=1 CANONICAL=D3ZYQ9-1"
    },
    "isoforms": [
        {
            "isoform_ac": "A0A8I6A812-1",
            "url": "",
            "sequence": {
                "sequence": "MSGIGNKRAAGEPGTSVPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQSKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLDQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVDLLSRKLNSGDNLIMEEAVQELNSFLAQENVRLQELTDLLQEKHHTMSQEFCKLQGKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDFEEVTTQNEKLKVELRSAVEEVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDAKDEPTELKQDSEDLATHSSALKASQEDEVKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDPVKDKDKGKHDDGRKKEAEIIKQLKTELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAVAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDLFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKVRSANCLCLLGLEVVH",
                "length": 931,
                "header": "tr|A0A8I6A812-1|A0A8I6A812_RAT Computationally mapped isoform of E3 ubiquitin protein ligase  OS=Rattus norvegicus OX=10116 GN=Rnf20 PE=1 SV=1 CANONICAL=D3ZYQ9-1"
            },
            "locus": {
                "chromosome": "5",
                "start_pos": 64000452,
                "end_pos": 63977320,
                "strand": "-",
                "evidence": [
                    {
                        "database": "Ensembl Transcript",
                        "id": "ENSRNOT00000105438.1",
                        "url": "https://www.ensembl.org/id/ENSRNOT00000105438.1"
                    },
                    {
                        "database": "Ensembl Peptide",
                        "id": "ENSRNOP00000088402.1",
                        "url": "https://www.ensembl.org/id/ENSRNOP00000088402.1"
                    }
                ]
            }
        },
        {
            "isoform_ac": "A0A8I6AJL7-1",
            "url": "",
            "sequence": {
                "sequence": "MFYSGNGKLDWSRVLDDSIEELDIRTLQSKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLDQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVDLLSRKLNSGDNLIMEEAVQELNSFLAQENVRLQELTDLLQEKHHTMSQEFCKLQGKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDFEEVTTQNEKLKVELRSAVEEVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDAKDEPTELKQDSEDLATHSSALKASQEDEVKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDPVKDKDKGKHDDGRKKEAEIIKQLKTELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAVAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDLFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG",
                "length": 949,
                "header": "tr|A0A8I6AJL7-1|D3ZYQ9_RAT Computationally mapped isoform of E3 ubiquitin protein ligase  OS=Rattus norvegicus OX=10116 GN=Rnf20 PE=1 SV=1 CANONICAL=D3ZYQ9-1"
            },
            "locus": {
                "chromosome": "5",
                "start_pos": 64000860,
                "end_pos": 63981401,
                "strand": "-",
                "evidence": [
                    {
                        "database": "Ensembl Transcript",
                        "id": "ENSRNOT00000096159.1",
                        "url": "https://www.ensembl.org/id/ENSRNOT00000096159.1"
                    },
                    {
                        "database": "Ensembl Peptide",
                        "id": "ENSRNOP00000094446.1",
                        "url": "https://www.ensembl.org/id/ENSRNOP00000094446.1"
                    }
                ]
            }
        },
        {
            "isoform_ac": "D3ZYQ9-1",
            "url": "",
            "sequence": {
                "sequence": "MSGIGNKRAAGEPGTSVPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQSKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLDQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVDLLSRKLNSGDNLIMEEAVQELNSFLAQENVRLQELTDLLQEKHHTMSQEFCKLQGKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDFEEVTTQNEKLKVELRSAVEEVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDAKDEPTELKQDSEDLATHSSALKASQEDEVKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDPVKDKDKGKHDDGRKKEAEIIKQLKTELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAVAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDLFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG",
                "length": 973,
                "header": "tr|D3ZYQ9-1|D3ZYQ9_RAT Isoform 1 of E3 ubiquitin protein ligase  OS=Rattus norvegicus OX=10116 GN=Rnf20 PE=1 SV=1 CANONICAL=D3ZYQ9-1"
            },
            "locus": {
                "chromosome": "5",
                "start_pos": 64000860,
                "end_pos": 63977320,
                "strand": "-",
                "evidence": [
                    {
                        "database": "Ensembl Transcript",
                        "id": "ENSRNOT00000059869.4",
                        "url": "https://www.ensembl.org/id/ENSRNOT00000059869.4"
                    },
                    {
                        "database": "Ensembl Peptide",
                        "id": "ENSRNOP00000056614.2",
                        "url": "https://www.ensembl.org/id/ENSRNOP00000056614.2"
                    }
                ]
            }
        }
    ],
    "glycosylation": [],
    "phosphorylation": [],
    "glycation": [],
    "keywords": [
        "enzyme"
    ],
    "publication": [
        {
            "title": "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites.",
            "journal": "Proc. Natl. Acad. Sci. U.S.A.",
            "date": "2006",
            "authors": "Hoffert JD, Pisitkun T, Wang G, Shen RF, Knepper MA",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "D3ZYQ9",
                    "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "16641100",
                    "url": "https://glygen.org/publication/PubMed/16641100"
                }
            ]
        },
        {
            "title": "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues.",
            "journal": "Nat. Commun.",
            "date": "2012",
            "authors": "Lundby A, Secher A, Lage K, Nordsborg NB, Dmytriyev A, Lundby C, Olsen JV",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "D3ZYQ9",
                    "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "22673903",
                    "url": "https://glygen.org/publication/PubMed/22673903"
                }
            ]
        },
        {
            "title": "Spinal RNF20-Mediated Histone H2B Monoubiquitylation Regulates mGluR5 Transcription for Neuropathic Allodynia.",
            "journal": "The Journal of neuroscience : the official journal of the Society for Neuroscience",
            "date": "2018",
            "authors": "Lai CY, Hsieh MC, Ho YC, Wang HH, Chou D, Wen YC, Yang PS, Cheng JK, Peng HY",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001101399",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001101399"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "30201771",
                    "url": "https://glygen.org/publication/PubMed/30201771"
                }
            ]
        }
    ],
    "pathway": [
        {
            "id": "rno:313216",
            "name": "",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+rno:313216",
            "resource": "KEGG Pathway"
        },
        {
            "id": "R-RNO-8866654",
            "name": "E3 ubiquitin ligases ubiquitinate target proteins",
            "url": "https://www.reactome.org/PathwayBrowser/#R-RNO-8866654",
            "resource": "Reactome",
            "description": "E3 ubiquitin ligases ubiquitinate target proteins"
        },
        {
            "id": "R-RNO-9013422",
            "name": "RHOBTB1 GTPase cycle",
            "url": "https://www.reactome.org/PathwayBrowser/#R-RNO-9013422",
            "resource": "Reactome",
            "description": "RHOBTB1 GTPase cycle"
        }
    ],
    "orthologs": [
        {
            "uniprot_canonical_ac": "Q5DTM8-1",
            "protein_name": "E3 ubiquitin-protein ligase BRE1A",
            "gene_name": "Rnf20",
            "tax_id": 10090,
            "organism": "Mus musculus",
            "sequence": {
                "sequence": "MSGIGNKRAAGEPGTSMPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQSKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLDQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGDGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVDLLSRKLNSGDNLIVEEAVQELNSFLAQENVRLQELTDLLQEKHHTMSQEFCKLQGKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDFEEVTTQNEKLKVELRSAVEEVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPTELKQDSEDLATHSSALKASQEDEVKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDSVKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDLFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG",
                "length": 973
            },
            "evidence": [
                {
                    "id": "45235926",
                    "url": "http://www.informatics.jax.org/homology/45235926",
                    "database": "MGI"
                }
            ],
            "common_name": "Mouse"
        },
        {
            "uniprot_canonical_ac": "Q5VTR2-1",
            "protein_name": "E3 ubiquitin-protein ligase BRE1A",
            "gene_name": "RNF20",
            "tax_id": 9606,
            "organism": "Homo sapiens",
            "sequence": {
                "sequence": "MSGIGNKRAAGEPGTSMPPEKKAAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSRKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQEKHRTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRLCELEKLRQDFEEVTTQNEKLKVELRSAVEQVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQHQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKPDSEDLSSQSSASKASQEDANEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDSAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG",
                "length": 975
            },
            "evidence": [
                {
                    "id": "45235926",
                    "url": "http://www.informatics.jax.org/homology/45235926",
                    "database": "MGI"
                }
            ],
            "common_name": "Human"
        }
    ],
    "go_annotation": {
        "categories": [
            {
                "name": "Biological Process",
                "total": 8,
                "go_terms": [
                    {
                        "name": "histone H2B conserved C-terminal lysine ubiquitination",
                        "id": "GO:0071894",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0071894",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "positive regulation of transcription by RNA polymerase II",
                        "id": "GO:0045944",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045944",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "regulation of DNA-templated transcription",
                        "id": "GO:0006355",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006355",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "negative regulation of cell migration",
                        "id": "GO:0030336",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030336",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "positive regulation of DNA-templated transcription",
                        "id": "GO:0045893",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045893",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "protein polyubiquitination",
                        "id": "GO:0000209",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0000209",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "ubiquitin-dependent protein catabolic process",
                        "id": "GO:0006511",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006511",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "histone monoubiquitination",
                        "id": "GO:0010390",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0010390",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            },
            {
                "name": "Molecular Function",
                "total": 11,
                "go_terms": [
                    {
                        "name": "chromatin binding",
                        "id": "GO:0003682",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003682",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "transcription coactivator activity",
                        "id": "GO:0003713",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003713",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "identical protein binding",
                        "id": "GO:0042802",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042802",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "metal ion binding",
                        "id": "GO:0046872",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046872",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "ubiquitin protein ligase binding",
                        "id": "GO:0031625",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0031625",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "ubiquitin protein ligase activity",
                        "id": "GO:0061630",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0061630",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "histone binding",
                        "id": "GO:0042393",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042393",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "mRNA 3'-UTR binding",
                        "id": "GO:0003730",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003730",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "ubiquitin-protein transferase activity",
                        "id": "GO:0004842",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0004842",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "p53 binding",
                        "id": "GO:0002039",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0002039",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "histone H2B C-terminal K residue ubiquitin ligase activity",
                        "id": "GO:0140850",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0140850",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            },
            {
                "name": "Cellular Component",
                "total": 5,
                "go_terms": [
                    {
                        "name": "nucleus",
                        "id": "GO:0005634",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005634",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "nucleoplasm",
                        "id": "GO:0005654",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005654",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "nucleolus",
                        "id": "GO:0005730",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005730",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "ubiquitin ligase complex",
                        "id": "GO:0000151",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0000151",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "HULC complex",
                        "id": "GO:0033503",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0033503",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZYQ9",
                                "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            }
        ]
    },
    "pro_annotation": [],
    "site_annotation": [
        {
            "start_pos": 393,
            "end_pos": 395,
            "annotation": "n_glycosylation_sequon"
        },
        {
            "start_pos": 509,
            "end_pos": 511,
            "annotation": "n_glycosylation_sequon"
        }
    ],
    "ptm_annotation": [],
    "interactions": [],
    "reactions": [
        {
            "id": "R-RNO-8942099",
            "name": "RNF20:RNF40 binds PAF complex, Ubiquitin:UBE2A,B (Ubiquitin:RAD6), WAC and Histone H2B",
            "equation": "",
            "summary": "This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>",
            "cellular_location": "nucleoplasm",
            "input_participant_list": [],
            "output_participant_list": [],
            "enzyme_list": [
                "Q8CJB9-1",
                "P63149-1",
                "D3ZYQ9-1"
            ],
            "evidence": [
                {
                    "id": "R-RNO-8942099",
                    "database": "Reactome",
                    "url": "https://www.reactome.org/PathwayBrowser/#R-RNO-8942099"
                }
            ]
        },
        {
            "id": "R-RNO-8942101",
            "name": "RNF20:RNF40 monoubiquitinates Histone H2B",
            "equation": "",
            "summary": "This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>",
            "cellular_location": "nucleoplasm",
            "input_participant_list": [],
            "output_participant_list": [],
            "enzyme_list": [
                "Q8CJB9-1",
                "P63149-1",
                "D3ZYQ9-1"
            ],
            "evidence": [
                {
                    "id": "R-RNO-8942101",
                    "database": "Reactome",
                    "url": "https://www.reactome.org/PathwayBrowser/#R-RNO-8942101"
                }
            ]
        },
        {
            "id": "R-RNO-9018778",
            "name": "RHOBTB1 binds interacting proteins at the endosome membrane",
            "equation": "",
            "summary": "This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>",
            "cellular_location": "cytosol, plasma membrane",
            "input_participant_list": [],
            "output_participant_list": [],
            "enzyme_list": [
                "Q62868-1",
                "O54735-1",
                "Q63644-1",
                "D3ZYQ9-1"
            ],
            "evidence": [
                {
                    "id": "R-RNO-9018778",
                    "database": "Reactome",
                    "url": "https://www.reactome.org/PathwayBrowser/#R-RNO-9018778"
                }
            ]
        }
    ],
    "reaction_participants": [],
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        {
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            "activity": [
                {
                    "ec_number": "2.3.2.27",
                    "activity": "S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.",
                    "evidence": [
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                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                        }
                    ]
                },
                {
                    "ec_number": "2.3.2.27",
                    "activity": "",
                    "evidence": [
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                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/D3ZYQ9"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "O54735",
            "gene_name": "Pde5a",
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                {
                    "ec_number": "3.1.4.-",
                    "activity": "",
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                            "url": "https://www.uniprot.org/uniprot/O54735"
                        }
                    ]
                },
                {
                    "ec_number": "3.1.4.35",
                    "activity": "3',5'-cyclic GMP + H2O = GMP + H(+).",
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                            "url": "https://www.uniprot.org/uniprot/O54735"
                        }
                    ]
                },
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            ]
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                {
                    "ec_number": "2.3.2.23",
                    "activity": "S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.",
                    "evidence": [
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                            "url": "https://www.uniprot.org/uniprot/P63149"
                        }
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                    "ec_number": "2.3.2.23",
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                        }
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            ]
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                {
                    "ec_number": "2.7.11.1",
                    "activity": "ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein].",
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                        }
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                        }
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                    "ec_number": "2.7.11.1",
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                        }
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                    "ec_number": "2.7.11.1",
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        {
            "name": "isoformset.uniprotkb",
            "label": "Isoforms"
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        {
            "name": "homologset.mgi",
            "label": "MGI Homologs"
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