{
    "uniprot_canonical_ac": "D3ZVU9-1",
    "uniprot_ac": "D3ZVU9",
    "uniprot_id": "NAT8L_RAT",
    "mass": {
        "chemical_mass": 32719.0,
        "monoisotopic_mass": 32719.0
    },
    "refseq": {
        "ac": "NP_001178610",
        "name": "N-acetylaspartate synthetase",
        "summary": "",
        "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001178610"
    },
    "gene_names": [
        {
            "name": "Nat8l",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "https://www.uniprot.org/uniprot/D3ZVU9",
            "id": "D3ZVU9"
        },
        {
            "name": "Nat8l",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001178610",
            "id": "NP_001178610"
        },
        {
            "name": "RGD1305719",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001178610",
            "id": "NP_001178610"
        }
    ],
    "protein_names": [
        {
            "name": "N-acetylaspartate synthetase",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "",
            "id": "D3ZVU9"
        },
        {
            "name": "2.3.1.17",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "",
            "id": "D3ZVU9"
        },
        {
            "name": "N-acetylaspartate synthetase",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001178610",
            "id": "NP_001178610"
        },
        {
            "name": "N-acetyltransferase 8-like protein",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "",
            "id": "D3ZVU9"
        },
        {
            "name": "NAA synthetase; N-acetyltransferase 8-like protein",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001178610",
            "id": "NP_001178610"
        },
        {
            "name": "NAA synthetase",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "",
            "id": "D3ZVU9"
        }
    ],
    "function": [
        {
            "annotation": "Asp-NAT/NAT8L and NAA immunoreactivities correlate very well in rat brain, with primary localization in neurons, including cortical pyramidal neurons and granule cells of the hippocampus.",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001178610",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001178610"
                },
                {
                    "database": "PubMed",
                    "id": "20385109",
                    "url": "https://glygen.org/publication/PubMed/20385109"
                }
            ]
        },
        {
            "annotation": "Catalyzes the synthesis of N-acetylaspartate acid (NAA) from L-aspartate and acetyl-CoA (PubMed:18621030). Promotes dopamine uptake by regulating TNF-alpha expression (By similarity). Attenuates methamphetamine-induced inhibition of dopamine uptake (By similarity).",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "D3ZVU9",
                    "url": "https://www.uniprot.org/uniprot/D3ZVU9#function"
                },
                {
                    "database": "PubMed",
                    "id": "18621030",
                    "url": "https://glygen.org/publication/PubMed/18621030"
                },
                {
                    "database": "UniProtKB",
                    "id": "Q3UGX3",
                    "url": "https://www.uniprot.org/uniprot/Q3UGX3#function"
                },
                {
                    "database": "UniProtKB",
                    "id": "Q8N9F0",
                    "url": "https://www.uniprot.org/uniprot/Q8N9F0#function"
                }
            ]
        },
        {
            "annotation": "The decrease in N-acetylaspartate following severe traumatic brain injury was characterized by profound mitochondrial malfunctioning, dramatic repression of Nat8l gene and concomitant increase in the ASPA gene and protein levels. Publication Status: Online-Only",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001178610",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001178610"
                },
                {
                    "database": "PubMed",
                    "id": "24515258",
                    "url": "https://glygen.org/publication/PubMed/24515258"
                }
            ]
        }
    ],
    "disease": [],
    "snv": [],
    "mutagenesis": [],
    "expression_tissue": [],
    "expression_disease": [],
    "species": [
        {
            "name": "Rattus norvegicus",
            "taxid": 10116,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "D3ZVU9",
                    "url": "https://www.uniprot.org/uniprot/D3ZVU9"
                }
            ],
            "common_name": "Rat"
        }
    ],
    "gene": [
        {
            "name": "Nat8l",
            "url": "https://www.uniprot.org/uniprot/D3ZVU9",
            "locus": {
                "chromosome": "14",
                "start_pos": 76756077,
                "end_pos": 76763411,
                "strand": "+",
                "evidence": [
                    {
                        "database": "Ensembl Gene",
                        "id": "ENSRNOG00000049351",
                        "url": "https://www.ensembl.org/id/ENSRNOG00000049351"
                    }
                ]
            }
        }
    ],
    "crossref": [
        {
            "id": "ENSRNOG00000049351",
            "url": "https://bgee.org/?page=gene&gene_id=ENSRNOG00000049351",
            "database": "Bgee",
            "categories": [
                "Gene Expression"
            ]
        },
        {
            "id": "cd04301",
            "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd04301",
            "database": "CDD",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "289727",
            "url": "https://www.ncbi.nlm.nih.gov/gene/289727",
            "database": "GeneID",
            "categories": [
                "Genome Annotation"
            ]
        },
        {
            "id": "IPR000182",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR000182",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "IPR016181",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR016181",
            "database": "InterPro",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "rno:289727",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+rno:289727",
            "database": "KEGG Pathway",
            "categories": [
                "Enzyme and Pathway"
            ]
        },
        {
            "id": "FHEGIME",
            "url": "https://omabrowser.org/oma/group/FHEGIME/",
            "database": "OMA",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "5358579at2759",
            "url": "http://www.orthodb.org/?query=5358579at2759",
            "database": "OrthoDB",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR13947",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR13947",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PTHR13947:SF11",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR13947:SF11",
            "database": "PANTHER",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PF00583",
            "url": "http://pfam.xfam.org/family/PF00583",
            "database": "Pfam",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "PR_D3ZVU9",
            "url": "http://purl.obolibrary.org/obo/PR_D3ZVU9",
            "database": "PRO",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "R-RNO-8963693",
            "url": "https://www.reactome.org/PathwayBrowser/#R-RNO-8963693",
            "database": "Reactome",
            "categories": [
                "Protein Classification"
            ]
        },
        {
            "id": "NP_001178610",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001178610",
            "database": "RefSeq",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "1305719",
            "url": "http://rgd.mcw.edu/tools/genes/genes_view.cgi?id=1305719",
            "database": "RGD",
            "categories": [
                "Organism-specific"
            ]
        },
        {
            "id": "14165",
            "url": "https://www.rhea-db.org/rhea/14165",
            "database": "Rhea",
            "categories": [
                "Enzyme and Pathway"
            ]
        },
        {
            "id": "D3ZVU9",
            "url": "https://www.uniprot.org/uniprot/D3ZVU9",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        },
        {
            "id": "D3ZVU9-1",
            "url": "https://www.uniprot.org/uniprot/D3ZVU9-1",
            "database": "UniProtKB",
            "categories": [
                "Protein"
            ]
        }
    ],
    "sequence": {
        "sequence": "MHCGPPDMVCETKIVATEDHEALPGAKKDALLAAAGAMWPPLPAAPGPAAAPPPAAGPQPHGGTGGAGPPEGRGVCIREFRAAEQEAARRIFYDGILERIPNTAFRGLRQHPRTQLLYALLAALCFAVTRSLLLTCLVPAGLLALRYYYSRKVILAYLECALHTDMADIEQYYMKPPGSCFWVAVLDGNVVGIVAARAHEEDNTVELLRMSVDSRFRGKGIAKALGRRVLEFAMLHNYSAVVLGTTAVKVAAHKLYESLGFRHMGASDHYVLPGMTLSLAERLFFQVRYHRYRLQLREE",
        "length": 299,
        "header": "sp|D3ZVU9-1|NAT8L_RAT Isoform 1 of N-acetylaspartate synthetase  OS=Rattus norvegicus OX=10116 GN=Nat8l PE=1 SV=1 CANONICAL=D3ZVU9-1"
    },
    "isoforms": [
        {
            "isoform_ac": "D3ZVU9-1",
            "url": "",
            "sequence": {
                "sequence": "MHCGPPDMVCETKIVATEDHEALPGAKKDALLAAAGAMWPPLPAAPGPAAAPPPAAGPQPHGGTGGAGPPEGRGVCIREFRAAEQEAARRIFYDGILERIPNTAFRGLRQHPRTQLLYALLAALCFAVTRSLLLTCLVPAGLLALRYYYSRKVILAYLECALHTDMADIEQYYMKPPGSCFWVAVLDGNVVGIVAARAHEEDNTVELLRMSVDSRFRGKGIAKALGRRVLEFAMLHNYSAVVLGTTAVKVAAHKLYESLGFRHMGASDHYVLPGMTLSLAERLFFQVRYHRYRLQLREE",
                "length": 299,
                "header": "sp|D3ZVU9-1|NAT8L_RAT Isoform 1 of N-acetylaspartate synthetase  OS=Rattus norvegicus OX=10116 GN=Nat8l PE=1 SV=1 CANONICAL=D3ZVU9-1"
            },
            "locus": {
                "chromosome": "14",
                "start_pos": 76762712,
                "end_pos": 76758459,
                "strand": "+",
                "evidence": [
                    {
                        "database": "Ensembl Transcript",
                        "id": "ENSRNOT00000074734",
                        "url": "https://www.ensembl.org/id/ENSRNOT00000074734"
                    },
                    {
                        "database": "Ensembl Peptide",
                        "id": "ENSRNOP00000066413",
                        "url": "https://www.ensembl.org/id/ENSRNOP00000066413"
                    }
                ]
            }
        }
    ],
    "glycosylation": [],
    "phosphorylation": [],
    "glycation": [],
    "keywords": [
        "enzyme"
    ],
    "publication": [
        {
            "title": "N-acetylaspartate synthesis in the brain: mitochondria vs. microsomes.",
            "journal": "Brain Res.",
            "date": "2008",
            "authors": "Ariyannur P.S., Madhavarao C.N., Namboodiri A.M.",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "D3ZVU9",
                    "url": "https://www.uniprot.org/uniprot/D3ZVU9"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "18621030",
                    "url": "https://glygen.org/publication/PubMed/18621030"
                }
            ]
        },
        {
            "title": "Methamphetamine-induced neuronal protein NAT8L is the NAA biosynthetic enzyme: implications for specialized acetyl coenzyme A metabolism in the CNS.",
            "journal": "Brain Res.",
            "date": "2010",
            "authors": "Ariyannur PS, Moffett JR, Manickam P, Pattabiraman N, Arun P, Nitta A, Nabeshima T, Madhavarao CN, Namboodiri AM",
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "D3ZVU9",
                    "url": "https://www.uniprot.org/uniprot/D3ZVU9"
                },
                {
                    "database": "RefSeq",
                    "id": "NP_001178610",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001178610"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "20385109",
                    "url": "https://glygen.org/publication/PubMed/20385109"
                }
            ]
        },
        {
            "title": "The molecular mechanisms affecting N-acetylaspartate homeostasis following experimental graded traumatic brain injury.",
            "journal": "Molecular medicine (Cambridge, Mass.)",
            "date": "2014",
            "authors": "Di Pietro V, Amorini AM, Tavazzi B, Vagnozzi R, Logan A, Lazzarino G, Signoretti S, Lazzarino G, Belli A",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001178610",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001178610"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "24515258",
                    "url": "https://glygen.org/publication/PubMed/24515258"
                }
            ]
        }
    ],
    "pathway": [
        {
            "id": "rno:289727",
            "name": "",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+rno:289727",
            "resource": "KEGG Pathway"
        },
        {
            "id": "R-RNO-8963693",
            "name": "Aspartate and asparagine metabolism",
            "url": "https://www.reactome.org/PathwayBrowser/#R-RNO-8963693",
            "resource": "Reactome",
            "description": "Aspartate and asparagine metabolism"
        }
    ],
    "orthologs": [
        {
            "uniprot_canonical_ac": "Q3UGX3-1",
            "protein_name": "N-acetylaspartate synthetase",
            "gene_name": "Nat8l",
            "tax_id": 10090,
            "organism": "Mus musculus",
            "sequence": {
                "sequence": "MHCGPPDMVCETKIVATEDHEALPGAKKDALLVAAGAMWPPLPAAPGPAAAPPPAAGPQPHGGTGGAGPPEGRGVCIREFRAAEQEAARRIFYDGILERIPNTAFRGLRQHPRTQLLYALLAALCFAVTRSLLLTCLVPAGLLALRYYYSRKVILAYLECALHTDMADIEQYYMKPPGSCFWVAVLDGNVVGIVAARAHEEDNTVELLRMSVDSRFRGKSIAKALGRRVLEFAMLHNYSAVVLGTTAVKVAAHKLYESLGFRHMGASDHYVLPGMTLSLAERLFFQVRYHRYRLQLREE",
                "length": 299
            },
            "evidence": [
                {
                    "id": "45237423",
                    "url": "http://www.informatics.jax.org/homology/45237423",
                    "database": "MGI"
                }
            ],
            "common_name": "Mouse"
        },
        {
            "uniprot_canonical_ac": "Q8N9F0-1",
            "protein_name": "N-acetylaspartate synthetase",
            "gene_name": "NAT8L",
            "tax_id": 9606,
            "organism": "Homo sapiens",
            "sequence": {
                "sequence": "MHCGPPDMVCETKIVAAEDHEALPGAKKDALLAAAGAMWPPLPAAPGPAAAPPAPPPAPVAQPHGGAGGAGPPGGRGVCIREFRAAEQEAARRIFYDGIMERIPNTAFRGLRQHPRAQLLYALLAALCFAVSRSLLLTCLVPAALLGLRYYYSRKVIRAYLECALHTDMADIEQYYMKPPGSCFWVAVLDGNVVGIVAARAHEEDNTVELLRMSVDSRFRGKGIAKALGRKVLEFAVVHNYSAVVLGTTAVKVAAHKLYESLGFRHMGASDHYVLPGMTLSLAERLFFQVRYHRYRLQLREE",
                "length": 302
            },
            "evidence": [
                {
                    "id": "45237423",
                    "url": "http://www.informatics.jax.org/homology/45237423",
                    "database": "MGI"
                },
                {
                    "id": "D3ZVU9",
                    "url": "https://omabrowser.org/oma/group/D3ZVU9/",
                    "database": "OMA"
                }
            ],
            "common_name": "Human"
        }
    ],
    "go_annotation": {
        "categories": [
            {
                "name": "Biological Process",
                "total": 3,
                "go_terms": [
                    {
                        "name": "positive regulation of dopamine uptake involved in synaptic transmission",
                        "id": "GO:0051586",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0051586",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZVU9",
                                "url": "https://www.uniprot.org/uniprot/D3ZVU9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "acetate metabolic process",
                        "id": "GO:0006083",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006083",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZVU9",
                                "url": "https://www.uniprot.org/uniprot/D3ZVU9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "aspartate metabolic process",
                        "id": "GO:0006531",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0006531",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZVU9",
                                "url": "https://www.uniprot.org/uniprot/D3ZVU9"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            },
            {
                "name": "Cellular Component",
                "total": 6,
                "go_terms": [
                    {
                        "name": "cytoplasm",
                        "id": "GO:0005737",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005737",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZVU9",
                                "url": "https://www.uniprot.org/uniprot/D3ZVU9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "mitochondrion",
                        "id": "GO:0005739",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005739",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZVU9",
                                "url": "https://www.uniprot.org/uniprot/D3ZVU9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "mitochondrial matrix",
                        "id": "GO:0005759",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005759",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZVU9",
                                "url": "https://www.uniprot.org/uniprot/D3ZVU9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "mitochondrial membrane",
                        "id": "GO:0031966",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0031966",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZVU9",
                                "url": "https://www.uniprot.org/uniprot/D3ZVU9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "intracellular membrane-bounded organelle",
                        "id": "GO:0043231",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043231",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZVU9",
                                "url": "https://www.uniprot.org/uniprot/D3ZVU9"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "endoplasmic reticulum membrane",
                        "id": "GO:0005789",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005789",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZVU9",
                                "url": "https://www.uniprot.org/uniprot/D3ZVU9"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            },
            {
                "name": "Molecular Function",
                "total": 1,
                "go_terms": [
                    {
                        "name": "aspartate N-acetyltransferase activity",
                        "id": "GO:0017188",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0017188",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "D3ZVU9",
                                "url": "https://www.uniprot.org/uniprot/D3ZVU9"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            }
        ]
    },
    "pro_annotation": [],
    "site_annotation": [
        {
            "start_pos": 237,
            "end_pos": 239,
            "annotation": "n_glycosylation_sequon"
        }
    ],
    "ptm_annotation": [],
    "interactions": [],
    "reactions": [
        {
            "id": "14165",
            "name": "",
            "equation": "acetyl-CoA + L-aspartate = CoA + H(+) + N-acetyl-L-aspartate",
            "summary": "",
            "cellular_location": "",
            "input_participant_list": [],
            "output_participant_list": [],
            "enzyme_list": [
                "D3ZVU9-1",
                "Q8N9F0-1",
                "Q3UGX3-1"
            ],
            "evidence": [
                {
                    "id": "14165",
                    "database": "Rhea",
                    "url": "https://www.rhea-db.org/rhea/14165"
                }
            ]
        },
        {
            "id": "R-RNO-8954468",
            "name": "NAT8L transfers acetyl group from Ac-CoA to L-Asp, forming NAA",
            "equation": "",
            "summary": "This event has been computationally inferred from an event that has been demonstrated in another species.<p>The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.<p><a href='/electronic_inference_compara.html' target = 'NEW'>More details and caveats of the event inference in Reactome.</a> For details on PANTHER see also: <a href='http://www.pantherdb.org/about.jsp' target='NEW'>http://www.pantherdb.org/about.jsp</a>",
            "cellular_location": "mitochondrial matrix",
            "input_participant_list": [],
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