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motifs: implications for vaccination and antibody therapeutics.", "journal": "Glycobiology", "date": "2020", "authors": "Zhou D, Tian X, Qi R, Peng C, Zhang W", "evidence": [ { "database": "UniCarbKB", "id": "GLY_000479", "url": "http://data.glygen.org/GLY_000479" }, { "database": "GlyConnect", "id": "2839", "url": "https://glyconnect.expasy.org/browser/proteins/2839" } ], "reference": [ { "type": "PubMed", "id": "32518941", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "title": "Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins", "journal": "Mol Cell Proteomics", "date": "2021", "authors": "Zhang Y., Zhao W., Mao Y., Chen Y., Wang S., Zhong Y., Su T., Gong M., Du D., Lu X., Cheng J., Yang H.", "evidence": [ { "database": "UniCarbKB", "id": "GLY_000479", "url": "http://data.glygen.org/GLY_000479" } ], "reference": [ { "type": "DOI", "id": "10.1074/mcp.RA120.002295", "url": 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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" 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"glytoucan_ac": "G60923RB", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 3", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" } ] }, { "glytoucan_ac": "G61627IG", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "2838", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2838" } ] }, { "glytoucan_ac": "G62595EF", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides before enrichment (trypsin digestion)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1101/2020.03.28.013276" } ] }, { "glytoucan_ac": "G62765YT", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-T-before-R1", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1101/2020.03.28.013276" } ] }, { "glytoucan_ac": "G62792OG", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides before enrichment (trypsin digestion)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1101/2020.03.28.013276" } ] }, { "glytoucan_ac": "G63543FL", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides before enrichment (trypsin digestion)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1101/2020.03.28.013276" } ] }, { "glytoucan_ac": "G64162JC", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "2839", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2839" } ] }, { "glytoucan_ac": "G64394MX", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "2838", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2838" } ] }, { "glytoucan_ac": "G64615IX", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides before enrichment (trypsin digestion)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1101/2020.03.28.013276" } ] }, { "glytoucan_ac": "G65184UU", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides before enrichment (trypsin digestion)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1101/2020.03.28.013276" } ] }, { "glytoucan_ac": "G66362RJ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides before enrichment (trypsin digestion)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1101/2020.03.28.013276" } ] }, { "glytoucan_ac": "G67367OT", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "S2P-AC", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33064451", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33064451" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1021/acs.analchem.0c03301" }, { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1101/2020.03.28.013276" } ] }, { "glytoucan_ac": "G67506FN", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "2838", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2838" } ] }, { "glytoucan_ac": "G68318VE", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "S2P-AC", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33064451", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33064451" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1021/acs.analchem.0c03301" }, { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" }, { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1101/2020.03.28.013276" } ] }, { "glytoucan_ac": "G69107AL", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" } ] }, { "glytoucan_ac": "G69364JQ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "S2P-AC", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33064451", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33064451" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1021/acs.analchem.0c03301" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" }, { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1101/2020.03.28.013276" } ] }, { "glytoucan_ac": "G70822IO", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G72787SB", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides before enrichment (trypsin digestion)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1101/2020.03.28.013276" } ] }, { "glytoucan_ac": "G72790NZ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides before enrichment (trypsin digestion)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1101/2020.03.28.013276" } ] }, { "glytoucan_ac": "G72791KH", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides before enrichment (trypsin digestion)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn657", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1101/2020.03.28.013276", "database": "DOI", "url": 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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G05724UK", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32363391", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32363391" }, { "id": "2838", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2838" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G06110VR", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32363391", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32363391" }, { "id": "2838", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2838" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" }, { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "2839", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2839" } ] }, { "glytoucan_ac": "G08011QI", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "2839", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2839" } ] }, { "glytoucan_ac": "G08293MJ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" } ] }, { "glytoucan_ac": "G10256JP", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G10339FR", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G15486FH", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G16407EV", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-G-before-R1", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G20956ZV", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Glycan database GlycomeDB; gLabel embedded in pGlyco2.0; Byonic (N-glycan 38 insect database)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" } ] }, { "glytoucan_ac": "G21726WW", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-G-after-R2", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" } ] }, { "glytoucan_ac": "G22040QI", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" }, { "id": "2839", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2839" } ] }, { "glytoucan_ac": "G22768VO", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": { "label": "iCn3D", "url": "https://www.ncbi.nlm.nih.gov/Structure/icn3d/?bcifid=8CYB&bu=0&command=select+.A:709;+center+selection;+zoom+selection" }, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "8CYB", "database": "PDB", "url": "https://www.rcsb.org/structure/8CYB" } ] }, { "glytoucan_ac": "G23453IV", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G25637MV", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "2839", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2839" } ] }, { "glytoucan_ac": "G25987BV", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G26759AS", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" }, { "id": "2839", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2839" } ] }, { "glytoucan_ac": "G27622TD", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-G-after-R1", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" } ] }, { "glytoucan_ac": "G28541PG", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" } ] }, { "glytoucan_ac": "G28681TP", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Glycan database GlycomeDB; gLabel embedded in pGlyco2.0; Byonic (N-glycan 38 insect database)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" } ] }, { "glytoucan_ac": "G28997IA", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-G-after-R1", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" } ] }, { "glytoucan_ac": "G31852PQ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G32104JU", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "2839", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2839" } ] }, { "glytoucan_ac": "G35029YA", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-G-before-R1", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G39188ZX", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32363391", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32363391" }, { "id": "2838", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2838" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G39446WN", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G41247ZX", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G44215PV", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" }, { "id": "2839", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2839" } ] }, { "glytoucan_ac": "G45504EY", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "SARS-CoV-2 S protein", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32363391", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32363391" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1101/2020.04.01.020966", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1101/2020.04.01.020966" } ] }, { "glytoucan_ac": "G46687AB", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G49874UX", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G53434XO", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-G-after-R1", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G57317CE", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-G-after-R2", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" } ] }, { "glytoucan_ac": "G57776ZU", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-TG-before-R2", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" } ] }, { "glytoucan_ac": "G59937CP", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "S2P-LC", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "33064451", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33064451" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1021/acs.analchem.0c03301" }, { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" } ] }, { "glytoucan_ac": "G62765YT", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G64527OM", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G66538GV", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn709", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G67324HN", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. 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{}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G23453IV", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G23863VK", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G25987BV", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Glycan database GlycomeDB; gLabel embedded in pGlyco2.0; Byonic (N-glycan 38 insect database)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" } ] }, { "glytoucan_ac": "G28541PG", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" } ] }, { "glytoucan_ac": "G28681TP", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Glycan database GlycomeDB; gLabel embedded in pGlyco2.0; Byonic (N-glycan 38 insect database)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" } ] }, { "glytoucan_ac": "G29880MM", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G30799SW", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" }, { "id": "2839", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2839" } ] }, { "glytoucan_ac": "G31852PQ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G33609NS", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G34029GR", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 3", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" } ] }, { "glytoucan_ac": "G35029YA", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 1", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G37399XV", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" } ] }, { "glytoucan_ac": "G39188ZX", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32363391", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32363391" }, { "id": "2838", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2838" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G39446WN", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G41247ZX", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G46687AB", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G50045TK", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G50073PQ", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 3", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" } ] }, { "glytoucan_ac": "G53434XO", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Glycan database GlycomeDB; gLabel embedded in pGlyco2.0; Byonic (N-glycan 38 insect database)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" } ] }, { "glytoucan_ac": "G54600FO", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 1", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" } ] }, { "glytoucan_ac": "G57776ZU", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" } ] }, { "glytoucan_ac": "G58802FE", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 3", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" } ] }, { "glytoucan_ac": "G59937CP", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" } ] }, { "glytoucan_ac": "G60145BJ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32363391", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32363391" }, { "id": "2838", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2838" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G60177UT", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G62765YT", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Glycan database GlycomeDB; gLabel embedded in pGlyco2.0; Byonic (N-glycan 38 insect database)", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" } ] }, { "glytoucan_ac": "G65184UU", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" } ] }, { "glytoucan_ac": "G66538GV", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G66766XF", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G67324HN", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" } ] }, { "glytoucan_ac": "G70101JE", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G72735IY", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G74430RZ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G79568CQ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G80475RE", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 1", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G80920RR", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 1", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" }, { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" } ] }, { "glytoucan_ac": "G81315DD", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": { "label": "iCn3D", "url": "https://www.ncbi.nlm.nih.gov/Structure/icn3d/?bcifid=6ZOW&bu=0&command=select+.B:717;+center+selection;+zoom+selection" }, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "6ZOW", "database": "PDB", "url": "https://www.rcsb.org/structure/6ZOW" }, { "id": "6ZP5", "database": "PDB", "url": "https://www.rcsb.org/structure/6ZP5" }, { "id": "6ZP7", "database": "PDB", "url": "https://www.rcsb.org/structure/6ZP7" }, { "id": "7TF8", "database": "PDB", "url": "https://www.rcsb.org/structure/7TF8" }, { "id": "7TL1", "database": "PDB", "url": "https://www.rcsb.org/structure/7TL1" }, { "id": "9B8F", "database": "PDB", "url": "https://www.rcsb.org/structure/9B8F" }, { "id": "9B9U", "database": "PDB", "url": "https://www.rcsb.org/structure/9B9U" }, { "id": "9BBK", "database": "PDB", "url": "https://www.rcsb.org/structure/9BBK" }, { "id": "9BEA", "database": "PDB", "url": "https://www.rcsb.org/structure/9BEA" } ] }, { "glytoucan_ac": "G82119TF", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn717", "evidence": [ { "id": "32363391", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32363391" }, { "id": "2838", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2838" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G82364UA", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Glycan database 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"N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. 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Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" } ] }, { "glytoucan_ac": "G72790NZ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" } ] }, { "glytoucan_ac": "G72791KH", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Biological Replicate 3", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" } ] }, { "glytoucan_ac": "G74430RZ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G74724QE", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "32518941", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32518941" }, { "id": "2839", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2839" }, { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" } ] }, { "glytoucan_ac": "G79568CQ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G80475RE", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "S2P-T", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "33064451", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33064451" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1021/acs.analchem.0c03301" }, { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "10.1126/science.abb9983", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1126/science.abb9983" } ] }, { "glytoucan_ac": "G80920RR", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-T-before-R1", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" } ] }, { "glytoucan_ac": "G80966KZ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "2839", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2839" } ] }, { "glytoucan_ac": "G81295CK", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G81315DD", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": { "label": "iCn3D", "url": "https://www.ncbi.nlm.nih.gov/Structure/icn3d/?bcifid=6ZOW&bu=0&command=select+.A:801;+center+selection;+zoom+selection" }, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "6ZOW", "database": "PDB", "url": "https://www.rcsb.org/structure/6ZOW" }, { "id": "6ZP5", "database": "PDB", "url": "https://www.rcsb.org/structure/6ZP5" }, { "id": "6ZP7", "database": "PDB", "url": "https://www.rcsb.org/structure/6ZP7" }, { "id": "7CN9", "database": "PDB", "url": "https://www.rcsb.org/structure/7CN9" }, { "id": "7TF8", "database": "PDB", "url": "https://www.rcsb.org/structure/7TF8" }, { "id": "8CYB", "database": "PDB", "url": "https://www.rcsb.org/structure/8CYB" }, { "id": "9B9U", "database": "PDB", "url": "https://www.rcsb.org/structure/9B9U" }, { "id": "9BBK", "database": "PDB", "url": "https://www.rcsb.org/structure/9BBK" }, { "id": "9BD9", "database": "PDB", "url": "https://www.rcsb.org/structure/9BD9" } ] }, { "glytoucan_ac": "G82020ZR", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "2839", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2839" } ] }, { "glytoucan_ac": "G82119TF", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G82364UA", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Identified intact N-glycopeptides with noted commentS-T-before-R1", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "33077685", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33077685" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1074/mcp.RA120.002295", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1074/mcp.RA120.002295" } ] }, { "glytoucan_ac": "G82463GQ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G83555HU", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" } ] }, { "glytoucan_ac": "G83646BJ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "S2P-LC", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "33064451", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33064451" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1021/acs.analchem.0c03301" } ] }, { "glytoucan_ac": "G84452RH", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "32363391", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32363391" }, { "id": "2838", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2838" }, { "id": "32366695", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/32366695" }, { "id": "2840", "database": "GlyConnect", "url": "https://glyconnect.expasy.org/browser/proteins/2840" } ] }, { "glytoucan_ac": "G85740DB", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "Elastase digestion of purified S protein, providing glycopeptide identification metrics from Byonic search. Expression construct included residues 1\u22121208 of the SARS-CoV-2 S (GenBank: MN908947) with proline substitutions at residues 986 and 987, a \u201cGSAS\u201d substitution at the furin cleavage site (residues 682\u2013685), a C-terminal T4 fibritin trimerization motif, an HRV3C protease cleavage site, a TwinStrepTag and an 8XHisTag.", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "34004174", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/34004174" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1016/j.celrep.2021.109179", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1016/j.celrep.2021.109179" } ] }, { "glytoucan_ac": "G88374WZ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "S2P-LC", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "33064451", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33064451" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": "http://data.glygen.org/GLY_000479" }, { "id": "10.1021/acs.analchem.0c03301", "database": "DOI", "url": "https://glygen.org/publication/DOI/10.1021/acs.analchem.0c03301" } ] }, { "glytoucan_ac": "G88725PI", "type": "N-linked;o-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "S2P-AC", "icn3d_view": {}, "start_aa": "Asn", "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn801", "evidence": [ { "id": "33064451", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/33064451" }, { "id": "GLY_000479", "database": "UniCarbKB", "url": 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