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        ]
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    {
        "title": "A nuclear 3'-5' exonuclease involved in mRNA degradation interacts with Poly(A) polymerase and the hnRNA protein Npl3p.",
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        "date": "2000-01",
        "authors": "Burkard K T, Butler J S",
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        ],
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            {
                "name": "Saccharomyces cerevisiae S288C",
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                    {
                        "database": "UniProtKB",
                        "id": "Q12149",
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                ],
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                "glygen_name": "Yeast",
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        ],
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                        "field": "o_linked_glycans",
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        ]
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    {
        "title": "Contribution of domain structure to the RNA 3' end processing and degradation functions of the nuclear exosome subunit Rrp6p.",
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        "date": "2003-09",
        "authors": "Phillips Seasson, Butler J Scott",
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            "id": "12923258",
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                ],
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        ],
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                "end_pos": 238,
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                "comment": "Temperature-sensitive mutant. Abolishes exonuclease activity and increases accumulation of 5.8S + 30 nt RNA, 5' ETSRNA, U24 + 3 nt RNA and poly(A)+ snoRNAs. No effect on subcellular localization.",
                "evidence": [
                    {
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                ],
                "uniprot_canonical_ac": "Q12149-1"
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            {
                "start_pos": 240,
                "end_pos": 240,
                "sequence_org": "E",
                "sequence_mut": "A",
                "comment": "Temperature-sensitive mutant. Abolishes exonuclease activity and increases accumulation of 5.8S + 30 nt RNA, 5' ETSRNA and U24 + 3 nt RNA.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "Q12149",
                        "url": "https://www.uniprot.org/uniprotkb/Q12149"
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                    {
                        "database": "PubMed",
                        "id": "12923258",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/12923258"
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                ],
                "uniprot_canonical_ac": "Q12149-1"
            },
            {
                "start_pos": 296,
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                "sequence_org": "D",
                "sequence_mut": "A",
                "comment": "Temperature-sensitive mutant. Abolishes exonuclease activity and increases accumulation of 5.8S + 30 nt RNA, 5' ETSRNA and U24 + 3 nt RNA. No effect on subcellular localization.",
                "evidence": [
                    {
                        "database": "UniProtKB",
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                    {
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                        "id": "12923258",
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                "uniprot_canonical_ac": "Q12149-1"
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            {
                "start_pos": 361,
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                "evidence": [
                    {
                        "database": "UniProtKB",
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                    {
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                        "id": "12923258",
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                "uniprot_canonical_ac": "Q12149-1"
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                    {
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                "uniprot_canonical_ac": "Q12149-1"
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            {
                "start_pos": 365,
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                "sequence_org": "D",
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                "comment": "Temperature-sensitive mutant. Abolishes exonuclease activity and increases accumulation of 5.8S + 30 nt RNA, 5' ETSRNA and U24 + 3 nt RNA.",
                "evidence": [
                    {
                        "database": "UniProtKB",
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                "uniprot_canonical_ac": "Q12149-1"
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            {
                "start_pos": 448,
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                "sequence_org": "W",
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                "comment": "No significant effects on growth at different temperatures, in vitro exonuclease activity and processing 5.8S rRNA, U24 snoRNA and ETSRNA.",
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                "comment": "No significant effects on growth rates at different temperatures, processing 5' ETSRNA and poly(A)+ snoRNAs, non-significant or moderate defects in 5.8S rRNA processing resulting in accumulation of 5.8S + 30 nt RNA, increased accumulation of extended forms of snR40 snoRNA and U24 + 3 nt snoRNA. Reduces exonuclease activity. No effect on subcellular localization.",
                "evidence": [
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                        "id": "Q12149",
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                        "url": "https://pubmed.ncbi.nlm.nih.gov/12923258"
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                "start_pos": 700,
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                "comment": "Results in cytoplasmic accumulation of the protein. No significant effects on processing 5.8S rRNA, U24 snoRNA and ETSRNA.",
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                        "id": "Q12149",
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