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            },
            {
                "start_pos": 410,
                "end_pos": 410,
                "sequence_org": "Y",
                "sequence_mut": "T",
                "comment": "Produces 65% lanosterol, 2% parkeol and 33% 9beta-delta7-lanosterol instead of cycloartenol. Produces 75% lanosterol, 0.6% parkeol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with V-481. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with N-477 or Q-477 and V-481.",
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            },
            {
                "start_pos": 469,
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                "sequence_org": "A",
                "sequence_mut": "V",
                "comment": "Produces lanosterol and achilleol A instead of cycloartenol.",
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            {
                "start_pos": 477,
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                "sequence_org": "H",
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                "comment": "Produces 88% lanosterol and 12% parkeol instead of cycloartenol. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410 and V-481.",
                "evidence": [
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            {
                "start_pos": 477,
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                "sequence_org": "H",
                "sequence_mut": "Q",
                "comment": "Produces 22% lanosterol, 73% parkeol and 5% 9beta-delta7-lanosterol instead of cycloartenol. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410 and V-481.",
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                "sequence_org": "I",
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                "comment": "Produces lanosterol and achilleol A instead of cycloartenol.",
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                "start_pos": 481,
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                "sequence_org": "I",
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                "comment": "Produces 24% lanosterol, 20% parkeol and 56% cycloartenol. Produces 75% lanosterol, 0.6% parkeol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410.",
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                "sequence_org": "Y",
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    {
        "title": "Directed evolution experiments reveal mutations at cycloartenol synthase residue His477 that dramatically alter catalysis.",
        "journal": "Organic letters",
        "date": "2002-12-12",
        "authors": "Segura Michael J R, Lodeiro Silvia, Meyer Michelle M, Patel Akash J, Matsuda Seiichi P T",
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                ],
                "common_name": "Mouse-ear cress",
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        ],
        "mutagenesis": [
            {
                "start_pos": 477,
                "end_pos": 477,
                "sequence_org": "H",
                "sequence_mut": "N",
                "comment": "Produces 88% lanosterol and 12% parkeol instead of cycloartenol. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410 and V-481.",
                "evidence": [
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            },
            {
                "start_pos": 477,
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                "sequence_org": "H",
                "sequence_mut": "Q",
                "comment": "Produces 22% lanosterol, 73% parkeol and 5% 9beta-delta7-lanosterol instead of cycloartenol. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410 and V-481.",
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            {
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                "comment": "Produces lanosterol instead of cycloartenol.",
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    {
        "title": "Oxidosqualene cyclase second-sphere residues profoundly influence the product profile.",
        "journal": "Chembiochem : a European journal of chemical biology",
        "date": "2004-11-05",
        "authors": "Lodeiro Silvia, Segura Michael J R, Stahl Martin, Schulz-Gasch Tanja, Matsuda Seiichi P T",
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        ],
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                "common_name": "Mouse-ear cress",
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        ],
        "mutagenesis": [
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                "start_pos": 410,
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            {
                "start_pos": 410,
                "end_pos": 410,
                "sequence_org": "Y",
                "sequence_mut": "T",
                "comment": "Produces 65% lanosterol, 2% parkeol and 33% 9beta-delta7-lanosterol instead of cycloartenol. Produces 75% lanosterol, 0.6% parkeol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with V-481. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with N-477 or Q-477 and V-481.",
                "evidence": [
                    {
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                        "id": "P38605",
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            },
            {
                "start_pos": 477,
                "end_pos": 477,
                "sequence_org": "H",
                "sequence_mut": "N",
                "comment": "Produces 88% lanosterol and 12% parkeol instead of cycloartenol. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410 and V-481.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "P38605",
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                    {
                        "database": "PubMed",
                        "id": "12081472",
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                    },
                    {
                        "database": "PubMed",
                        "id": "12465912",
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                    {
                        "database": "PubMed",
                        "id": "15515077",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/15515077"
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                "uniprot_canonical_ac": "P38605-1"
            },
            {
                "start_pos": 477,
                "end_pos": 477,
                "sequence_org": "H",
                "sequence_mut": "Q",
                "comment": "Produces 22% lanosterol, 73% parkeol and 5% 9beta-delta7-lanosterol instead of cycloartenol. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410 and V-481.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "P38605",
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                    {
                        "database": "PubMed",
                        "id": "12081472",
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            {
                "start_pos": 477,
                "end_pos": 477,
                "sequence_org": "H",
                "sequence_mut": "Y",
                "comment": "Produces lanosterol instead of cycloartenol.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "P38605",
                        "url": "https://www.uniprot.org/uniprotkb/P38605"
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                        "id": "12081472",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/12081472"
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                    {
                        "database": "PubMed",
                        "id": "12465912",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/12465912"
                    },
                    {
                        "database": "PubMed",
                        "id": "15515077",
                        "url": "https://pubmed.ncbi.nlm.nih.gov/15515077"
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                ],
                "uniprot_canonical_ac": "P38605-1"
            },
            {
                "start_pos": 481,
                "end_pos": 481,
                "sequence_org": "I",
                "sequence_mut": "T",
                "comment": "Produces lanosterol and achilleol A instead of cycloartenol.",
                "evidence": [
                    {
                        "database": "UniProtKB",
                        "id": "P38605",
                        "url": "https://www.uniprot.org/uniprotkb/P38605"
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                        "id": "12081472",
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            {
                "start_pos": 481,
                "end_pos": 481,
                "sequence_org": "I",
                "sequence_mut": "V",
                "comment": "Produces 24% lanosterol, 20% parkeol and 56% cycloartenol. Produces 75% lanosterol, 0.6% parkeol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410.",
                "evidence": [
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        "title": "Allelic mutant series reveal distinct functions for Arabidopsis cycloartenol synthase 1 in cell viability and plastid biogenesis.",
        "journal": "Proceedings of the National Academy of Sciences of the United States of America",
        "date": "2008-02-26",
        "authors": "Babiychuk Elena, Bouvier-Nav\u00e9 Pierrette, Compagnon Vincent, Suzuki Masashi, Muranaka Toshiya, Van Montagu Marc, Kushnir Sergei, Schaller Hubert",
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        "title": "Isolation of an Arabidopsis thaliana gene encoding cycloartenol synthase by functional expression in a yeast mutant lacking lanosterol synthase by the use of a chromatographic screen.",
        "journal": "Proceedings of the National Academy of Sciences of the United States of America",
        "date": "1993-12-15",
        "authors": "Corey E J, Matsuda S P, Bartel B",
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    {
        "title": "The Arabidopsis rab5 homologs rha1 and ara7 localize to the prevacuolar compartment.",
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                    {
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                    {
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                    {
                        "database": "PubMed",
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                    {
                        "database": "PubMed",
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                        "url": "https://pubmed.ncbi.nlm.nih.gov/20833725"
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        "title": "Direct metal recognition by guanine nucleotide-exchange factor in the initial step of the exchange reaction.",
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