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"attached", "comment": "", "icn3d_view": {}, "start_pos": 563, "start_aa": "Asn", "end_pos": 563, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn563", "evidence": [ { "id": "35927436", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/35927436" }, { "id": "TP3222006", "database": "GlyTableMaker", "url": "https://glygen.ccrc.uga.edu/tablemaker/data/TP3222006" } ] }, { "glytoucan_ac": "G70101JE", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_pos": 563, "start_aa": "Asn", "end_pos": 563, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn563", "evidence": [ { "id": "35927436", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/35927436" }, { "id": "TP3222006", "database": "GlyTableMaker", "url": "https://glygen.ccrc.uga.edu/tablemaker/data/TP3222006" } ] }, { "glytoucan_ac": "G72735IY", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_pos": 563, "start_aa": "Asn", "end_pos": 563, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn563", "evidence": [ { "id": "35927436", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/35927436" }, { "id": "TP3222006", "database": "GlyTableMaker", "url": "https://glygen.ccrc.uga.edu/tablemaker/data/TP3222006" } ] }, { "glytoucan_ac": "", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported", "site_category_dict": { "reported": true }, "site_seq": "N", "relation": "attached", "comment": "N-linked (GlcNAc...) asparagine", "icn3d_view": {}, "start_pos": 563, "start_aa": "Asn", "end_pos": 563, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn563", "evidence": [ { "id": "Q05320", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprotkb/Q05320#ptm_processing" } ] } ], "mutagenesis": [ { "sequence_org": "N", "sequence_mut": "D", "comment": "Reduced levels of expression of GP, GP1 and GP2. 20% loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "618", "end_pos": "618", "site_lbl": "Asn618", "weight": 0.0, "glycosylation": [ { "glytoucan_ac": "G08249BH", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_pos": 618, "start_aa": "Asn", "end_pos": 618, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn618", "evidence": [ { "id": "35927436", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/35927436" }, { "id": "TP3222006", "database": "GlyTableMaker", "url": "https://glygen.ccrc.uga.edu/tablemaker/data/TP3222006" } ] }, { "glytoucan_ac": "G60145BJ", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_pos": 618, "start_aa": "Asn", "end_pos": 618, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn618", "evidence": [ { "id": "35927436", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/35927436" }, { "id": "TP3222006", "database": "GlyTableMaker", "url": "https://glygen.ccrc.uga.edu/tablemaker/data/TP3222006" } ] }, { "glytoucan_ac": "G68094RR", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_pos": 618, "start_aa": "Asn", "end_pos": 618, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn618", "evidence": [ { "id": "35927436", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/35927436" }, { "id": "TP3222006", "database": "GlyTableMaker", "url": "https://glygen.ccrc.uga.edu/tablemaker/data/TP3222006" } ] }, { "glytoucan_ac": "G91344EV", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported_with_glycan", "site_category_dict": { "reported_with_glycan": true }, "site_seq": "N", "relation": "attached", "comment": "", "icn3d_view": {}, "start_pos": 618, "start_aa": "Asn", "end_pos": 618, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn618", "evidence": [ { "id": "35927436", "database": "PubMed", "url": "https://glygen.org/publication/PubMed/35927436" }, { "id": "TP3222006", "database": "GlyTableMaker", "url": "https://glygen.ccrc.uga.edu/tablemaker/data/TP3222006" } ] }, { "glytoucan_ac": "", "type": "N-linked", "subtype": "", "prediction_tool_list": [], "site_category": "reported", "site_category_dict": { "reported": true }, "site_seq": "N", "relation": "attached", "comment": "N-linked (GlcNAc...) asparagine", "icn3d_view": {}, "start_pos": 618, "start_aa": "Asn", "end_pos": 618, "end_aa": "Asn", "residue": "Asn", "site_lbl": "Asn618", "evidence": [ { "id": "Q05320", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprotkb/Q05320#ptm_processing" } ] } ], "mutagenesis": [ { "sequence_org": "N", "sequence_mut": "D", "comment": "Slightly reduced levels of expression of GP1 and GP2. No loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "108", "end_pos": "108", "site_lbl": "Cys108", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "G", "comment": "Almost complete loss of expression of GP1 and GP2. Almost complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "111", "end_pos": "111", "site_lbl": "Leu111", "weight": 0.0, "mutagenesis": [ { "sequence_org": "L", "sequence_mut": "A", "comment": "About 60% loss of ability to counteract host BST2.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "27707924", "url": "https://glygen.org/publication/PubMed/27707924" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "121", "end_pos": "121", "site_lbl": "Cys121", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "G", "comment": "Reduced levels of expression of GP1 and GP2. 50% loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "122", "end_pos": "122", "site_lbl": "Leu122", "weight": 0.0, "mutagenesis": [ { "sequence_org": "L", "sequence_mut": "A", "comment": "About 60% loss of ability to counteract host BST2.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "27707924", "url": "https://glygen.org/publication/PubMed/27707924" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "135", "end_pos": "135", "site_lbl": "Cys135", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "S", "comment": "Almost complete loss of expression of GP1 and GP2. Complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "147", "end_pos": "147", "site_lbl": "Cys147", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "S", "comment": "Reduced levels of expression of GP1 and GP2. Almost complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "170", "end_pos": "170", "site_lbl": "Ile170", "weight": 0.0, "mutagenesis": [ { "sequence_org": "I", "sequence_mut": "A", "comment": "90% loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] }, { "sequence_org": "I", "sequence_mut": "E", "comment": "Complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] }, { "sequence_org": "I", "sequence_mut": "F", "comment": "50% loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "511", "end_pos": "511", "site_lbl": "Cys511", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "G", "comment": "Induces GP1 secretion. Complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "528", "end_pos": "528", "site_lbl": "Gly528", "weight": 0.0, "mutagenesis": [ { "sequence_org": "G", "sequence_mut": "R", "comment": "Reduced infectivity.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "10482652", "url": "https://glygen.org/publication/PubMed/10482652" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "529", "end_pos": "529", "site_lbl": "Leu529", "weight": 0.0, "mutagenesis": [ { "sequence_org": "L", "sequence_mut": "A", "comment": "Reduced infectivity.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "10482652", "url": "https://glygen.org/publication/PubMed/10482652" } ] }, { "sequence_org": "L", "sequence_mut": "R", "comment": "Reduced infectivity.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "10482652", "url": "https://glygen.org/publication/PubMed/10482652" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "532", "end_pos": "532", "site_lbl": "Ile532", "weight": 0.0, "mutagenesis": [ { "sequence_org": "I", "sequence_mut": "A", "comment": "Reduced infectivity.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "10482652", "url": "https://glygen.org/publication/PubMed/10482652" } ] }, { "sequence_org": "I", "sequence_mut": "R", "comment": "Almost complete loss of infectivity. 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No effect on transport of GP to the cell surface and incorporation onto virions.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "10482652", "url": "https://glygen.org/publication/PubMed/10482652" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "537", "end_pos": "537", "site_lbl": "Pro537", "weight": 0.0, "mutagenesis": [ { "sequence_org": "P", "sequence_mut": "R", "comment": "Almost complete loss of infectivity. 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Complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "608", "end_pos": "608", "site_lbl": "Cys608", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "G", "comment": "Induces GP1 secretion. Complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "609", "end_pos": "609", "site_lbl": "Cys609", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "G", "comment": "Induces GP1 secretion. Complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "632", "end_pos": "632", "site_lbl": "Asp632", "weight": 0.0, "mutagenesis": [ { "sequence_org": "D", "sequence_mut": "V", "comment": "No effect on release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "633", "end_pos": "633", "site_lbl": "Lys633", "weight": 0.0, "mutagenesis": [ { "sequence_org": "K", "sequence_mut": "R", "comment": "No effect on release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] }, { "sequence_org": "K", "sequence_mut": "V", "comment": "No effect on release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "634", "end_pos": "634", "site_lbl": "Thr634", "weight": 0.0, "mutagenesis": [ { "sequence_org": "T", "sequence_mut": "I", "comment": "50% loss of release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "635", "end_pos": "635", "site_lbl": "Leu635", "weight": 0.0, "mutagenesis": [ { "sequence_org": "L", "sequence_mut": "V", "comment": "60% loss of release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] }, { "sequence_org": "L", "sequence_mut": "V", "comment": "Increased GP shedding.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "26092855", "url": "https://glygen.org/publication/PubMed/26092855" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "636", "end_pos": "636", "site_lbl": "Pro636", "weight": 0.0, "mutagenesis": [ { "sequence_org": "P", "sequence_mut": "A", "comment": "60% loss of release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "637", "end_pos": "637", "site_lbl": "Asp637", "weight": 0.0, "mutagenesis": [ { "sequence_org": "D", "sequence_mut": "E", "comment": "No effect on release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] }, { "sequence_org": "D", "sequence_mut": "L", "comment": "Increased release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] }, { "sequence_org": "D", "sequence_mut": "V", "comment": "Decreased GP shedding.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "26092855", "url": "https://glygen.org/publication/PubMed/26092855" } ] }, { "sequence_org": "D", "sequence_mut": "V", "comment": "Increased release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "638", "end_pos": "638", "site_lbl": "Gln638", "weight": 0.0, "mutagenesis": [ { "sequence_org": "Q", "sequence_mut": "V", "comment": "No effect on release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "639", "end_pos": "639", "site_lbl": "Gly639", "weight": 0.0, "mutagenesis": [ { "sequence_org": "G", "sequence_mut": "V", "comment": "40% loss of release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "63", "end_pos": "63", "site_lbl": "Leu63", "weight": 0.0, "mutagenesis": [ { "sequence_org": "L", "sequence_mut": "A", "comment": "90% loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] }, { "sequence_org": "L", "sequence_mut": "F", "comment": "Almost complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] }, { "sequence_org": "L", "sequence_mut": "K", "comment": "Complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "640", "end_pos": "640", "site_lbl": "Asp640", "weight": 0.0, "mutagenesis": [ { "sequence_org": "D", "sequence_mut": "V", "comment": "No effect on release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "641", "end_pos": "641", "site_lbl": "Asn641", "weight": 0.0, "mutagenesis": [ { "sequence_org": "N", "sequence_mut": "A", "comment": "No effect on release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "642", "end_pos": "642", "site_lbl": "Asp642", "weight": 0.0, "mutagenesis": [ { "sequence_org": "D", "sequence_mut": "V", "comment": "No effect on release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "643", "end_pos": "643", "site_lbl": "Asn643", "weight": 0.0, "mutagenesis": [ { "sequence_org": "N", "sequence_mut": "A", "comment": "No effect on release of soluble GP1,2delta.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "15103332", "url": "https://glygen.org/publication/PubMed/15103332" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "64", "end_pos": "64", "site_lbl": "Arg64", "weight": 0.0, "mutagenesis": [ { "sequence_org": "R", "sequence_mut": "A", "comment": "Complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] }, { "sequence_org": "R", "sequence_mut": "E", "comment": "Complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] }, { "sequence_org": "R", "sequence_mut": "K", "comment": "No loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "660", "end_pos": "660", "site_lbl": "Gly660", "weight": 0.0, "mutagenesis": [ { "sequence_org": "G", "sequence_mut": "L", "comment": "About 60% loss of viral release; when associated with L-664.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "29669839", "url": "https://glygen.org/publication/PubMed/29669839" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "664", "end_pos": "664", "site_lbl": "Ala664", "weight": 0.0, "mutagenesis": [ { "sequence_org": "A", "sequence_mut": "L", "comment": "About 60% loss of viral release; when associated with L-660.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "29669839", "url": "https://glygen.org/publication/PubMed/29669839" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "670", "end_pos": "670", "site_lbl": "Cys670", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "A", "comment": "Reduced palmitoylation. No effect on GP processing and association with retrovirus particle. No loss of virus capability to enter into host cell. Loss of localization to the rafts; when associated with A-670.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "11877482", "url": "https://glygen.org/publication/PubMed/11877482" }, { "database": "PubMed", "id": "11152533", "url": "https://glygen.org/publication/PubMed/11152533" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] }, { "sequence_org": "C", "sequence_mut": "F", "comment": "Slightly reduced levels of expression of GP1 and GP2. Greatly reduced GP processing and association with retrovirus particle. 43% loss of virus capability to enter into host cell. Loss of localization to the rafts; when associated with A-672.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "11877482", "url": "https://glygen.org/publication/PubMed/11877482" }, { "database": "PubMed", "id": "11152533", "url": "https://glygen.org/publication/PubMed/11152533" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "672", "end_pos": "672", "site_lbl": "Cys672", "weight": 0.0, "mutagenesis": [ { "sequence_org": "C", "sequence_mut": "A", "comment": "Reduced palmitoylation. No effect on GP processing and association with retrovirus particle. No loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "11152533", "url": "https://glygen.org/publication/PubMed/11152533" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] }, { "sequence_org": "C", "sequence_mut": "F", "comment": "Slightly reduced levels of expression of GP1 and GP2. Almost no effect on GP processing and association with retrovirus particle. 24% loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "11152533", "url": "https://glygen.org/publication/PubMed/11152533" }, { "database": "PubMed", "id": "12438572", "url": "https://glygen.org/publication/PubMed/12438572" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "88", "end_pos": "88", "site_lbl": "Phe88", "weight": 0.0, "mutagenesis": [ { "sequence_org": "F", "sequence_mut": "A", "comment": "About 50% loss of ability to counteract host BST2.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" }, { "database": "PubMed", "id": "27707924", "url": "https://glygen.org/publication/PubMed/27707924" } ] }, { "sequence_org": "F", "sequence_mut": "A", "comment": "Complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] }, { "sequence_org": "F", "sequence_mut": "E", "comment": "Complete loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] }, { "sequence_org": "F", "sequence_mut": "I", "comment": "No loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] } ] }, { "uniprot_canonical_ac": "Q05320-1", "start_pos": "95", "end_pos": "95", "site_lbl": "Lys95", "weight": 0.0, "mutagenesis": [ { "sequence_org": "K", "sequence_mut": "A", "comment": "80% loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] }, { "sequence_org": "K", "sequence_mut": "E", "comment": "80% loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] }, { "sequence_org": "K", "sequence_mut": "R", "comment": "20% loss of virus capability to enter into host cell.", "evidence": [ { "database": "UniProtKB", "id": "Q05320", "url": "https://www.uniprot.org/uniprotkb/Q05320" } ] } ] } ], "record_id": "batch.ebov.1" }, { "uniprot_canonical_ac": "Q05322-1", "species": [ { "name": "Zaire ebolavirus (strain Mayinga-76)", "taxid": 128952, "evidence": [ { "database": "UniProtKB", "id": "Q05322", "url": "https://www.uniprot.org/uniprotkb/Q05322" } ], "common_name": "Zaire ebolavirus", "glygen_name": "Ebola (EBOV)", "reference_species": "Zaire ebolavirus (strain Mayinga-76) [128952]" } ], "length": 251, "chemical_mass": 28219.0, "gene_names": "VP24", "protein_name": "Membrane-associated protein VP24", "disease": [], "cellular_component": [ { "name": "membrane", "id": "GO:0016020", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016020", "evidence": [ { "database": "UniProtKB", "id": "Q05322", "url": "https://www.uniprot.org/uniprotkb/Q05322" } ], "pmid": "" }, { "name": "virion membrane", "id": "GO:0055036", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0055036", "evidence": [ { "database": "UniProtKB", "id": "Q05322", "url": "https://www.uniprot.org/uniprotkb/Q05322" } ], "pmid": "" }, { "name": "host cell plasma membrane", "id": "GO:0020002", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0020002", "evidence": [ { "database": "UniProtKB", "id": "Q05322", "url": "https://www.uniprot.org/uniprotkb/Q05322" } ], "pmid": "" }, { "name": "host cell endomembrane system", "id": "GO:0033645", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0033645", "evidence": [ { "database": "UniProtKB", "id": "Q05322", "url": "https://www.uniprot.org/uniprotkb/Q05322" } ], "pmid": "" } ], "molecular_function": [ { "name": "structural molecule activity", "id": "GO:0005198", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005198", "evidence": [ { "database": "UniProtKB", "id": "Q05322", "url": "https://www.uniprot.org/uniprotkb/Q05322" } ], "pmid": "" } ], "biological_process": [ { "name": "symbiont-mediated suppression of host type I interferon-mediated signaling pathway", "id": "GO:0039502", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0039502", "evidence": [ { "database": "UniProtKB", "id": "Q05322", "url": "https://www.uniprot.org/uniprotkb/Q05322" } ], "pmid": "" }, { "name": "symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity", "id": "GO:0039563", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0039563", "evidence": [ { "database": "UniProtKB", "id": "Q05322", "url": "https://www.uniprot.org/uniprotkb/Q05322" } ], "pmid": "" }, { "name": "viral budding from plasma membrane", "id": "GO:0046761", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046761", "evidence": [ { "database": "UniProtKB", "id": "Q05322", "url": "https://www.uniprot.org/uniprotkb/Q05322" } ], "pmid": "" }, { "name": "symbiont-mediated suppression of host innate immune response", "id": "GO:0052170", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0052170", "evidence": [ { "database": "UniProtKB", "id": "Q05322", "url": "https://www.uniprot.org/uniprotkb/Q05322" } ], "pmid": "" } ], "binding_glycans": [], "biomarkers": [], "sites": [ { "uniprot_canonical_ac": "Q05322-1", "start_pos": "137", "end_pos": "137", "site_lbl": "Arg137", "weight": 0.0, "mutagenesis": [ { "sequence_org": "R", "sequence_mut": "A", "comment": "More than 90% loss of interaction with host KPNA5.", "evidence": [ { "database": "UniProtKB", "id": "Q05322", "url": "https://www.uniprot.org/uniprotkb/Q05322" }, { "database": "PubMed", "id": "25121748", "url": "https://glygen.org/publication/PubMed/25121748" } ] } ] }, { "uniprot_canonical_ac": "Q05322-1", "start_pos": "170", "end_pos": "170", "site_lbl": "Val170", "weight": 0.0, "mutagenesis": [ { "sequence_org": 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