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Lethal mitotic defects; when associated with A-11; A-12; A-22 and A-75.", "evidence": [ { "database": "UniProtKB", "id": "P0DJH6", "url": "https://www.uniprot.org/uniprotkb/P0DJH6" }, { "database": "PubMed", "id": "22304917", "url": "https://glygen.org/publication/PubMed/22304917" }, { "database": "PubMed", "id": "19070575", "url": "https://glygen.org/publication/PubMed/19070575" } ] } ] } ], "record_id": "P0DJH6-1" }, { "uniprot_canonical_ac": "P12003-2", "species": [ { "name": "Gallus gallus", "taxid": 9031, "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "common_name": "Chicken", "glygen_name": "Chicken", "reference_species": "Gallus gallus [9031]" } ], "length": 1135, "chemical_mass": 124560.0, "gene_names": "VCL;VCL;VINC1", "protein_name": "Vinculin", "disease": [], "molecular_function": [ { "name": "muscle alpha-actinin binding", "id": "GO:0051371", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0051371", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "protein homodimerization activity", "id": "GO:0042803", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042803", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "actin filament binding", "id": "GO:0051015", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0051015", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "ubiquitin protein ligase binding", "id": "GO:0031625", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0031625", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "alpha-actinin binding", "id": "GO:0051393", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0051393", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "structural molecule activity", "id": "GO:0005198", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005198", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "cadherin binding", "id": "GO:0045296", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045296", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "dystroglycan binding", "id": "GO:0002162", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0002162", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "beta-catenin binding", "id": "GO:0008013", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0008013", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "scaffold protein binding", "id": "GO:0097110", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0097110", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "vinculin binding", "id": "GO:0017166", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0017166", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "alpha-catenin binding", "id": "GO:0045294", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045294", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" } ], "biological_process": [ { "name": "apical junction assembly", "id": "GO:0043297", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043297", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "regulation of focal adhesion assembly", "id": "GO:0051893", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0051893", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "regulation of establishment of endothelial barrier", "id": "GO:1903140", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:1903140", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "cell adhesion", "id": "GO:0007155", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0007155", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "lamellipodium assembly", "id": "GO:0030032", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030032", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "regulation of cell migration", "id": "GO:0030334", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030334", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "protein localization to cell surface", "id": "GO:0034394", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0034394", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "adherens junction assembly", "id": "GO:0034333", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0034333", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "morphogenesis of an epithelium", "id": "GO:0002009", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0002009", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "epithelial cell-cell adhesion", "id": "GO:0090136", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0090136", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "regulation of protein localization to adherens junction", "id": "GO:1904702", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:1904702", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" } ], "cellular_component": [ { "name": "outer dense plaque of desmosome", "id": "GO:0090636", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0090636", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "inner dense plaque of desmosome", "id": "GO:0090637", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0090637", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "podosome ring", "id": "GO:0061826", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0061826", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "cytoplasm", "id": "GO:0005737", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005737", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "mitochondrial inner membrane", "id": "GO:0005743", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005743", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "cytoskeleton", "id": "GO:0005856", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005856", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "plasma membrane", "id": "GO:0005886", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005886", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "brush border", "id": "GO:0005903", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005903", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "cell-cell junction", "id": "GO:0005911", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005911", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "adherens junction", "id": "GO:0005912", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005912", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "focal adhesion", "id": "GO:0005925", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005925", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "actin cytoskeleton", "id": "GO:0015629", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0015629", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "neuromuscular junction", "id": "GO:0031594", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0031594", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "perinuclear region of cytoplasm", "id": "GO:0048471", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0048471", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "stress fiber", "id": "GO:0001725", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0001725", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "sarcolemma", "id": "GO:0042383", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042383", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "Z disc", "id": "GO:0030018", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030018", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "fascia adherens", "id": "GO:0005916", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005916", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ], "pmid": "" }, { "name": "cell-cell contact zone", "id": "GO:0044291", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0044291", "evidence": [ { "database": 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Little change in cell spreading. Complete loss of phosphorylation. No change in subcellular location nor on in vitro actin binding. 40% decrease in cell spreading; when associated with F-1134.", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" }, { "database": "PubMed", "id": "15229287", "url": "https://glygen.org/publication/PubMed/15229287" } ] } ] }, { "uniprot_canonical_ac": "P12003-2", "start_pos": "1134", "end_pos": "1134", "site_lbl": "Tyr1134", "weight": 0.0, "phosphorylation": [ { "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" }, { "database": "iPTMnet", "id": "P12003", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/P12003" }, { "database": "PubMed", "id": "15229287", "url": "https://glygen.org/publication/PubMed/15229287" } ] } ], "mutagenesis": [ { "sequence_org": "Y", "sequence_mut": "F", "comment": "Greatly reduced phosphorylation levels in platelets. Little change in cell spreading. Complete loss of phosphorylation. No change in subcellular location nor on in vitro actin binding. 40% decrease in cell spreading; when associated with F-100.", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" }, { "database": "PubMed", "id": "15229287", "url": "https://glygen.org/publication/PubMed/15229287" } ] } ] }, { "uniprot_canonical_ac": "P12003-2", "start_pos": "537", "end_pos": "537", "site_lbl": "Tyr537", "weight": 0.0, "phosphorylation": [ { "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" } ] } ], "mutagenesis": [ { "sequence_org": "Y", "sequence_mut": "F", "comment": "No change of phosphorylation levels in platelets.", "evidence": [ { "database": "UniProtKB", "id": "P12003", "url": "https://www.uniprot.org/uniprotkb/P12003" }, { "database": "PubMed", "id": "15229287", "url": "https://glygen.org/publication/PubMed/15229287" } ] } ] }, 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