{ "uniprot_canonical_ac": "A2ASQ1-1", "uniprot_ac": "A2ASQ1", "uniprot_id": "AGRIN_MOUSE", "mass": { "chemical_mass": 207539.0, "monoisotopic_mass": 207539.0 }, "refseq": { "ac": "NP_067617", "name": "agrin isoform 1 precursor", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_067617" }, "gene_names": [ { "name": "Agrn", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/A2ASQ1", "id": "A2ASQ1" }, { "name": "Agrin", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/A2ASQ1", "id": "A2ASQ1" } ], "protein_names": [ { "name": "Agrin", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/A2ASQ1#names_and_taxonomy", "id": "A2ASQ1" }, { "name": "agrin isoform 1 precursor", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_067617", "id": "NP_067617" } ], "function": [ { "annotation": "Agrin expression is correlated with tumor development and poor prognosis in cholangiocarcinoma.", "evidence": [ { "database": "RefSeq", "id": "NP_067617", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_067617" }, { "database": "PubMed", "id": "34018826", "url": "https://glygen.org/publication/PubMed/34018826" } ] }, { "annotation": "Isoform 2 and isoform 3: these isoforms lacking the 'z' insert (z0) are muscle-specific, have no AChR clustering ability and may be involved in nervous system endothelial cell differentiation.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1#function" } ] }, { "annotation": "The extracellular matrix protein Agrin is expressed by osteoblasts and contributes to their differentiation.", "evidence": [ { "database": "RefSeq", "id": "NP_067617", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_067617" }, { "database": "PubMed", "id": "34223979", "url": "https://glygen.org/publication/PubMed/34223979" } ] }, { "annotation": "heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. This neuron-specific (z+) isoform is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This transmembrane agrin (TM-agrin) isoform, the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1#function" } ] }, { "annotation": "involved in regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. Also involved in modulation of growth factor signaling (By similarity).", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1#function" }, { "database": "PubMed", "id": "8653787", "url": "https://glygen.org/publication/PubMed/8653787" }, { "database": "PubMed", "id": "21885656", "url": "https://glygen.org/publication/PubMed/21885656" }, { "database": "PubMed", "id": "19303856", "url": "https://glygen.org/publication/PubMed/19303856" }, { "database": "PubMed", "id": "11018052", "url": "https://glygen.org/publication/PubMed/11018052" }, { "database": "PubMed", "id": "10402191", "url": "https://glygen.org/publication/PubMed/10402191" }, { "database": "PubMed", "id": "17611272", "url": "https://glygen.org/publication/PubMed/17611272" }, { "database": "PubMed", "id": "12796478", "url": "https://glygen.org/publication/PubMed/12796478" } ] }, { "annotation": "this released fragment is important for agrin signaling and to exert a maximal dendritic filopodia-inducing effect. All 'z' splice variants (z+) of this fragment also show an increase in the number of filopodia.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1#function" } ] } ], "disease": [ { "disease_id": "DOID:0110657", "recommended_name": { "id": "DOID:0110657", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:0110657", "name": "congenital myasthenic syndrome 8", "description": "A congenital myasthenic syndrome characterized by autosomal recessive inheritance of prominent defects of both the pre- and postsynaptic regions and muscle weakness that has_material_basis_in homozygous or compound heterozygous mutation in the AGRN gene on chromosome 1p." }, "synonyms": [ { "id": "DOID:0110657", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:0110657", "name": "congenital myasthenic syndrome 8 with pre- and postsynaptic defects", "description": "" }, { "id": "DOID:0110657", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:0110657", "name": "CMS8", "description": "" }, { "id": "DOID:0110657", "resource": "Disease Ontology", "url": "http://disease-ontology.org/term/DOID:0110657", "name": "congenital myasthenic syndrome due to agrin deficiency", "description": "" }, { "id": "MONDO:0014052", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0014052", "name": "congenital myasthenic syndrome 8", "description": "Any congenital myasthenic syndrome in which the cause of the disease is a mutation in the AGRN gene." }, { "id": "MONDO:0014052", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0014052", "name": "congenital myasthenic syndrome type 8", "description": "" }, { "id": "MONDO:0014052", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0014052", "name": "myasthenic syndrome, congenital, with Pre- and postsynaptic defects", "description": "" }, { "id": "MONDO:0014052", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0014052", "name": "congenital myasthenic syndrome 8 with pre- and postsynaptic defects", "description": "" }, { "id": "MONDO:0014052", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0014052", "name": "myasthenic syndrome, congenital, 8; CMS8", "description": "" }, { "id": "MONDO:0014052", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0014052", "name": "myasthenic syndrome, congenital, type 8", "description": "" }, { "id": "MONDO:0014052", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0014052", "name": "congenital myasthenic syndrome due to agrin deficiency", "description": "" }, { "id": "MONDO:0014052", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0014052", "name": "AGRN congenital myasthenic syndrome", "description": "" }, { "id": "MONDO:0014052", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0014052", "name": "congenital myasthenic syndrome caused by mutation in AGRN", "description": "" }, { "id": "MONDO:0014052", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0014052", "name": "myasthenic syndrome, congenital, 8", "description": "" }, { "id": "MONDO:0014052", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0014052", "name": "myasthenic syndrome, congenital, due to agrin deficiency", "description": "" }, { "id": "MONDO:0014052", "resource": "MONDO", "url": "https://monarchinitiative.org/disease/MONDO:0014052", "name": "CMS8", "description": "" }, { "id": "MIM:615120", "resource": "MIM", "url": "https://www.omim.org/entry/615120", "name": "Myasthenic syndrome, congenital, 8", "description": "Myasthenic syndrome, congenital, 8" } ], "evidence": [ { "id": "0110657", "database": "Alliance of Genome Resources", "url": "https://www.alliancegenome.org/disease/DOID:0110657" } ] } ], "snv": [], "mutagenesis": [], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Mus musculus", "taxid": 10090, "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "common_name": "Mouse" } ], "gene": [ { "name": "Agrn", "url": "https://www.uniprot.org/uniprot/A2ASQ1", "locus": { "chromosome": "4", "start_pos": 156197488, "end_pos": 156165290, "strand": "-", "evidence": [ { "database": "Ensembl Gene", "id": "ENSMUSG00000041936", "url": "https://www.ensembl.org/id/ENSMUSG00000041936" } ] } } ], "crossref": [ { "id": "ENSMUSG00000041936", "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000041936", "database": "Bgee" }, { "id": "11603", "url": "https://www.ncbi.nlm.nih.gov/gene/11603", "database": "GeneID" }, { "id": "GPDB0001727", "url": "https://acgg.asia/gpdb2/GPDB0001727", "database": "GlycoProtDB" }, { "id": "GPDB0001728", "url": "https://acgg.asia/gpdb2/GPDB0001728", "database": "GlycoProtDB" }, { "id": "GPDB0001729", "url": "https://acgg.asia/gpdb2/GPDB0001729", "database": "GlycoProtDB" }, { "id": "GPDB0003029", "url": "https://acgg.asia/gpdb2/GPDB0003029", "database": "GlycoProtDB" }, { "id": "GPDB0007936", "url": "https://acgg.asia/gpdb2/GPDB0007936", "database": "GlycoProtDB" }, { "id": "GPDB0007941", "url": "https://acgg.asia/gpdb2/GPDB0007941", "database": "GlycoProtDB" }, { "id": "A2ASQ1", "url": "https://www.ebi.ac.uk/intact/interactors/id:A2ASQ1*", "database": "IntAct" }, { "id": "IPR000082", "url": "https://www.ebi.ac.uk/interpro/entry/IPR000082", "database": "InterPro" }, { "id": "IPR000742", "url": "https://www.ebi.ac.uk/interpro/entry/IPR000742", "database": "InterPro" }, { "id": "IPR001791", "url": "https://www.ebi.ac.uk/interpro/entry/IPR001791", "database": "InterPro" }, { "id": "IPR001881", "url": "https://www.ebi.ac.uk/interpro/entry/IPR001881", "database": "InterPro" }, { "id": "IPR002049", "url": "https://www.ebi.ac.uk/interpro/entry/IPR002049", "database": "InterPro" }, { "id": "IPR002350", "url": "https://www.ebi.ac.uk/interpro/entry/IPR002350", "database": "InterPro" }, { "id": "IPR003645", "url": "https://www.ebi.ac.uk/interpro/entry/IPR003645", "database": "InterPro" }, { "id": "IPR003884", "url": "https://www.ebi.ac.uk/interpro/entry/IPR003884", "database": "InterPro" }, { "id": "IPR004850", "url": "https://www.ebi.ac.uk/interpro/entry/IPR004850", "database": "InterPro" }, { "id": "IPR008993", "url": "https://www.ebi.ac.uk/interpro/entry/IPR008993", "database": "InterPro" }, { "id": "IPR013320", "url": "https://www.ebi.ac.uk/interpro/entry/IPR013320", "database": "InterPro" }, { "id": "IPR036058", "url": "https://www.ebi.ac.uk/interpro/entry/IPR036058", "database": "InterPro" }, { "id": "IPR036364", "url": "https://www.ebi.ac.uk/interpro/entry/IPR036364", "database": "InterPro" }, { "id": "87961", "url": "http://www.informatics.jax.org/marker/MGI:87961", "database": "MGI" }, { "id": "MFLCQCP", "url": "https://omabrowser.org/oma/group/MFLCQCP/", "database": "OMA" }, { "id": "414294at2759", "url": "http://www.orthodb.org/?query=414294at2759", "database": "OrthoDB" }, { "id": "3PVE", "url": "https://www.rcsb.org/structure/3PVE", "database": "PDB" }, { "id": "PF00008", "url": "http://pfam.xfam.org/family/PF00008", "database": "Pfam" }, { "id": "PF00050", "url": "http://pfam.xfam.org/family/PF00050", "database": "Pfam" }, { "id": "PF00053", "url": "http://pfam.xfam.org/family/PF00053", "database": "Pfam" }, { "id": "PF00054", "url": "http://pfam.xfam.org/family/PF00054", "database": "Pfam" }, { "id": "PF01390", "url": "http://pfam.xfam.org/family/PF01390", "database": "Pfam" }, { "id": "PF03146", "url": "http://pfam.xfam.org/family/PF03146", "database": "Pfam" }, { "id": "PF07648", "url": "http://pfam.xfam.org/family/PF07648", "database": "Pfam" }, { "id": "PR_A2ASQ1", "url": "http://purl.obolibrary.org/obo/PR_A2ASQ1", "database": "PRO" }, { "id": "R-MMU-1971475", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-1971475", "database": "Reactome" }, { "id": "R-MMU-2022928", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-2022928", "database": "Reactome" }, { "id": "R-MMU-2024096", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-2024096", "database": "Reactome" }, { "id": "R-MMU-3000178", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-3000178", "database": "Reactome" }, { "id": "R-MMU-975634", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-975634", "database": "Reactome" }, { "id": "NP_067617", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_067617", "database": "RefSeq" }, { "id": "XP_011248479", "url": "https://www.ncbi.nlm.nih.gov/protein/XP_011248479", "database": "RefSeq" }, { "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1", "database": "UniProtKB" }, { "id": "A2ASQ1-1", "url": "https://www.uniprot.org/uniprot/A2ASQ1-1", "database": "UniProtKB" }, { "id": "A2ASQ1-2", "url": "https://www.uniprot.org/uniprot/A2ASQ1-2", "database": "UniProtKB" }, { "id": "A2ASQ1-3", "url": "https://www.uniprot.org/uniprot/A2ASQ1-3", "database": "UniProtKB" }, { "id": "M0QWP1", "url": "https://www.uniprot.org/uniprot/M0QWP1", "database": "UniProtKB" }, { "id": "M0QWP1-1", "url": "https://www.uniprot.org/uniprot/M0QWP1-1", "database": "UniProtKB" }, { "id": "Z4YJS5", "url": "https://www.uniprot.org/uniprot/Z4YJS5", "database": "UniProtKB" }, { "id": "Z4YJS5-1", "url": "https://www.uniprot.org/uniprot/Z4YJS5-1", "database": "UniProtKB" }, { "id": "Z4YK85", "url": "https://www.uniprot.org/uniprot/Z4YK85", "database": "UniProtKB" }, { "id": "Z4YK85-1", "url": "https://www.uniprot.org/uniprot/Z4YK85-1", "database": "UniProtKB" } ], "sequence": { "sequence": "MPPLPLEHRPRQQPGASVLVRYFMIPCNICLILLATSTLGFAVLLFLSNYKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL", "length": 1950, "header": "sp|A2ASQ1-1|AGRIN_MOUSE Isoform 1 of Agrin OS=Mus musculus OX=10090 GN=Agrn PE=1 SV=1 CANONICAL=A2ASQ1-1" }, "isoforms": [ { "isoform_ac": "A2ASQ1-1", "url": "https://www.uniprot.org/uniprot/A2ASQ1#A2ASQ1-1", "sequence": { "sequence": "MPPLPLEHRPRQQPGASVLVRYFMIPCNICLILLATSTLGFAVLLFLSNYKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL", "length": 1950, "header": "sp|A2ASQ1-1|AGRIN_MOUSE Isoform 1 of Agrin OS=Mus musculus OX=10090 GN=Agrn PE=1 SV=1 CANONICAL=A2ASQ1-1" }, "locus": { "chromosome": "4", "start_pos": 156270155, "end_pos": 156250956, "strand": "+", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000105575", "url": "https://www.ensembl.org/id/ENSMUST00000105575" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000101200", "url": "https://www.ensembl.org/id/ENSMUSP00000101200" } ] } }, { "isoform_ac": "A2ASQ1-2", "url": "https://www.uniprot.org/uniprot/A2ASQ1#A2ASQ1-2", "sequence": { "sequence": "MPPLPLEHRPRQQPGASVLVRYFMIPCNICLILLATSTLGFAVLLFLSNYKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL", "length": 1928, "header": "sp|A2ASQ1-2|AGRIN_MOUSE Isoform 2 of Agrin OS=Mus musculus OX=10090 GN=Agrn PE=1 SV=1 CANONICAL=A2ASQ1-1" }, "locus": {} }, { "isoform_ac": "A2ASQ1-3", "url": "https://www.uniprot.org/uniprot/A2ASQ1#A2ASQ1-3", "sequence": { "sequence": "MPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESSEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL", "length": 1867, "header": "sp|A2ASQ1-3|AGRIN_MOUSE Isoform 3 of Agrin OS=Mus musculus OX=10090 GN=Agrn PE=1 SV=1 CANONICAL=A2ASQ1-1" }, "locus": {} }, { "isoform_ac": "M0QWP1-1", "url": "https://www.uniprot.org/uniprot/A2ASQ1#M0QWP1-1", "sequence": { "sequence": "MVRPRLSFPAPLLPLLLLLAAAAPAVPGASGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVVIGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL", "length": 2034, "header": "tr|M0QWP1-1|AGRIN_MOUSE Computationally mapped isoform of Agrin OS=Mus musculus OX=10090 GN=Agrn PE=1 SV=1 CANONICAL=A2ASQ1-1" }, "locus": { "chromosome": "4", "start_pos": 156281877, "end_pos": 156250956, "strand": "+", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000180572", "url": "https://www.ensembl.org/id/ENSMUST00000180572" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000137931", "url": "https://www.ensembl.org/id/ENSMUSP00000137931" } ] } }, { "isoform_ac": "Z4YJS5-1", "url": "https://www.uniprot.org/uniprot/A2ASQ1#Z4YJS5-1", "sequence": { "sequence": "MPPLPLEHRPRQQPGASVLVRYFMIPCNICLILLATSTLGFAVLLFLSNYKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDPAPLSELPLELPPSLL", "length": 1151, "header": "tr|Z4YJS5-1|Z4YJS5_MOUSE Computationally mapped isoform of Agrin OS=Mus musculus OX=10090 GN=Agrn PE=1 SV=1 CANONICAL=A2ASQ1-1" }, "locus": { "chromosome": "4", "start_pos": 156270155, "end_pos": 156250922, "strand": "+", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000071248", "url": "https://www.ensembl.org/id/ENSMUST00000071248" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000071229", "url": "https://www.ensembl.org/id/ENSMUSP00000071229" } ] } }, { "isoform_ac": "Z4YK85-1", "url": "https://www.uniprot.org/uniprot/A2ASQ1#Z4YK85-1", "sequence": { "sequence": "MPPLPLEHRPRQQPGASVLVRYFMIPCNICLILLATSTLGFAVLLFLSNYKPGIHFTAAPSMPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSPSTTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEPPVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQIQVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPVGRTTAPLTTRRPPTTTASIDRPRTPGPQRPPKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL", "length": 1927, "header": "tr|Z4YK85-1|Z4YK85_MOUSE Computationally mapped isoform of Agrin OS=Mus musculus OX=10090 GN=Agrn PE=1 SV=1 CANONICAL=A2ASQ1-1" }, "locus": { "chromosome": "4", "start_pos": 156270155, "end_pos": 156250956, "strand": "+", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000105574", "url": "https://www.ensembl.org/id/ENSMUST00000105574" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000101199", "url": "https://www.ensembl.org/id/ENSMUSP00000101199" } ] } } ], "glycosylation": [ { "glytoucan_ac": "", "type": "N-linked", "subtype": "", "site_category": "predicted", "site_seq": "N", "relation": "attached", "comment": "N-linked (GlcNAc...) asparagine", "start_pos": 145, "start_aa": "Asn", "end_pos": 145, "end_aa": "Asn", "residue": "Asn", "evidence": [ { "id": "A2ASQ1", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/A2ASQ1#ptm_processing" } ] }, { "glytoucan_ac": "", "type": "O-linked", "subtype": "O-fucosylation", "site_category": "predicted", "site_seq": "S", "relation": "attached", "comment": "O-linked (Fuc...) serine O-fucosylation", "start_pos": 1717, "start_aa": "Ser", "end_pos": 1717, "end_aa": "Ser", "residue": "Ser", "evidence": [ { "id": "A2ASQ1", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/A2ASQ1#ptm_processing" } ] }, { "glytoucan_ac": "", "type": "N-linked", "subtype": "", "site_category": "predicted", "site_seq": "N", "relation": "attached", "comment": "N-linked (GlcNAc...) asparagine", "start_pos": 672, "start_aa": "Asn", "end_pos": 672, "end_aa": "Asn", "residue": "Asn", "evidence": [ { "id": "A2ASQ1", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/A2ASQ1#ptm_processing" } ] }, { "glytoucan_ac": "", "type": "N-linked", "subtype": "", "site_category": "predicted", "site_seq": "N", "relation": "attached", "comment": "N-linked (GlcNAc...) asparagine", "start_pos": 827, "start_aa": "Asn", "end_pos": 827, "end_aa": "Asn", "residue": "Asn", "evidence": [ { "id": "A2ASQ1", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/A2ASQ1#ptm_processing" } ] }, { "glytoucan_ac": "", "type": "N-linked", "subtype": "", "site_category": "predicted", "site_seq": "N", "relation": "attached", "comment": "N-linked (GlcNAc...) asparagine", "start_pos": 948, "start_aa": "Asn", "end_pos": 948, "end_aa": "Asn", "residue": "Asn", "evidence": [ { "id": "A2ASQ1", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/A2ASQ1#ptm_processing" } ] } ], "phosphorylation": [ { "start_pos": 569, "end_pos": 569, "kinase_uniprot_canonical_ac": "Q3UD01-1", "kinase_gene_name": "Atxn7l3b", "residue": "Ser", "comment": "", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" }, { "database": "iPTMnet", "id": "A2ASQ1", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/A2ASQ1" }, { "database": "PubMed", "id": "21183079", "url": "https://glygen.org/publication/PubMed/21183079" } ] }, { "start_pos": 569, "end_pos": 569, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "Phosphoserine", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" }, { "database": "PubMed", "id": "21183079", "url": "https://glygen.org/publication/PubMed/21183079" } ] }, { "start_pos": 571, "end_pos": 571, "kinase_uniprot_canonical_ac": "Q3UD01-1", "kinase_gene_name": "Atxn7l3b", "residue": "Ser", "comment": "", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" }, { "database": "iPTMnet", "id": "A2ASQ1", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/A2ASQ1" }, { "database": "PubMed", "id": "21183079", "url": "https://glygen.org/publication/PubMed/21183079" } ] }, { "start_pos": 571, "end_pos": 571, "kinase_uniprot_canonical_ac": "", "kinase_gene_name": "", "residue": "Ser", "comment": "Phosphoserine", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" }, { "database": "PubMed", "id": "21183079", "url": "https://glygen.org/publication/PubMed/21183079" } ] } ], "glycation": [], "keywords": [ "glycoprotein", "glycoprotein_predicted" ], "publication": [ { "title": "Alternatively spliced isoforms of nerve- and muscle-derived agrin: their roles at the neuromuscular junction.", "journal": "Neuron", "date": "1999", "authors": "Sanes J.R., Burgess R.W., Lichtman J.W., Nguyen Q.T., Son Y.J.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "10402191", "url": "https://glygen.org/publication/PubMed/10402191" } ] }, { "title": "Interaction of agrin with laminin requires a coiled-coil conformation of the agrin-binding site within the laminin gamma1 chain.", "journal": "EMBO J.", "date": "1999", "authors": "Engel J., Lustig A., Denzer A.J., Ruegg M.A., Schumacher B., Kammerer R.A., Yurchenco P.D., Landwehr R., Schulthess T.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "10581249", "url": "https://glygen.org/publication/PubMed/10581249" } ] }, { "title": "Agrin isoforms with distinct amino termini: differential expression, localization, and function.", "journal": "J. Cell Biol.", "date": "2000", "authors": "Sanes J.R., Burgess R.W., Skarnes W.C.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "11018052", "url": "https://glygen.org/publication/PubMed/11018052" } ] }, { "title": "An alternative amino-terminus expressed in the central nervous system converts agrin to a type II transmembrane protein.", "journal": "Mol. Cell. Neurosci.", "date": "2001", "authors": "Annies M., Bittcher G., Kroger S., Neumann F.R., Ruegg M.A., Schumacher B.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "11161480", "url": "https://glygen.org/publication/PubMed/11161480" } ] }, { "title": "The COOH-terminal domain of agrin signals via a synaptic receptor in central nervous system neurons.", "journal": "J. Cell Biol.", "date": "2003", "authors": "Smith M.A., Hilgenberg L.G., Hoover C.L.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "12796478", "url": "https://glygen.org/publication/PubMed/12796478" } ] }, { "title": "Comprehensive identification of phosphorylation sites in postsynaptic density preparations.", "journal": "Mol. Cell. Proteomics", "date": "2006", "authors": "Schoepfer R., Burlingame A.L., Thalhammer A., Specht C.G., Trinidad J.C.", "evidence": [ { "database": "UniProtKB", "id": "M0QWP1", "url": "https://www.uniprot.org/uniprot/M0QWP1" }, { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" }, { "database": "UniProtKB", "id": "Z4YK85", "url": "https://www.uniprot.org/uniprot/Z4YK85" }, { "database": "UniProtKB", "id": "Z4YJS5", "url": "https://www.uniprot.org/uniprot/Z4YJS5" } ], "reference": [ { "type": "PubMed", "id": "16452087", "url": "https://glygen.org/publication/PubMed/16452087" } ] }, { "title": "Synapse loss in cortex of agrin-deficient mice after genetic rescue of perinatal death.", "journal": "J. Neurosci.", "date": "2007", "authors": "Burkhardt C., Lin S., Ruegg M.A., Sanes J.R., Maj M., Bettler B., Seddik R., Arber S., Caroni P., Bezakova G., Jucker M., Ksiazek I.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "17611272", "url": "https://glygen.org/publication/PubMed/17611272" } ] }, { "title": "Neurotrypsin cleaves agrin locally at the synapse.", "journal": "FASEB J.", "date": "2008", "authors": "Kozlov S.V., Kunz B., Sonderegger P., Cinelli P., Stephan A., Hettwer S., Kistler A.D., Mateos J.M., Rulicke T., Streit P.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "18230682", "url": "https://glygen.org/publication/PubMed/18230682" } ] }, { "title": "Rescuing Z+ agrin splicing in Nova null mice restores synapse formation and unmasks a physiologic defect in motor neuron firing.", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "date": "2009", "authors": "Darnell R.B., Burden S.J., Kim N., Ruggiu M., Herbst R., Fak J.J., Fischbach G., Jevsek M., Mann M.A.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "19221030", "url": "https://glygen.org/publication/PubMed/19221030" } ] }, { "title": "Coincident pre- and postsynaptic activation induces dendritic filopodia via neurotrypsin-dependent agrin cleavage.", "journal": "Cell", "date": "2009", "authors": "Kunz B., Sonderegger P., Zurlinden A., Matsumoto-Miyai K., Gee C.E., Gerber U., Hettwer S., Ladner A.P., Luscher D., Rulicke T., Sokolowska E., Ster J., Wolfel J.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "19303856", "url": "https://glygen.org/publication/PubMed/19303856" } ] }, { "title": "Structural and biophysical characterisation of agrin laminin G3 domain constructs.", "journal": "Protein Eng. Des. Sel.", "date": "2011", "authors": "Tidow H., Nissen P., Mattle D.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "21037280", "url": "https://glygen.org/publication/PubMed/21037280" } ] }, { "title": "A tissue-specific atlas of mouse protein phosphorylation and expression.", "journal": "Cell", "date": "2010", "authors": "Huttlin EL, Jedrychowski MP, Elias JE, Goswami T, Rad R, Beausoleil SA, Villen J, Haas W, Sowa ME, Gygi SP", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" }, { "database": "iPTMnet", "id": "A2ASQ1", "url": "http://research.bioinformatics.udel.edu/iptmnet/entry/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "21183079", "url": "https://glygen.org/publication/PubMed/21183079" } ] }, { "title": "Destabilization of the neuromuscular junction by proteolytic cleavage of agrin results in precocious sarcopenia.", "journal": "FASEB J.", "date": "2011", "authors": "Bolliger M.F., Kunz B., Sonderegger P., Zurlinden A., Butikofer L.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "21885656", "url": "https://glygen.org/publication/PubMed/21885656" } ] }, { "title": "A valid mouse model of AGRIN-associated congenital myasthenic syndrome.", "journal": "Hum. Mol. Genet.", "date": "2011", "authors": "Burgess R.W., Bogdanik L.P.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "21890498", "url": "https://glygen.org/publication/PubMed/21890498" } ] }, { "title": "Agrin expression is correlated with tumor development and poor prognosis in cholangiocarcinoma.", "journal": "The Journal of international medical research", "date": "2021", "authors": "He M, Cheng C, Tu J, Ji SS, Lou D, Bai B", "evidence": [ { "database": "RefSeq", "id": "NP_067617", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_067617" } ], "reference": [ { "type": "PubMed", "id": "34018826", "url": "https://glygen.org/publication/PubMed/34018826" } ] }, { "title": "The extracellular matrix protein Agrin is expressed by osteoblasts and contributes to their differentiation.", "journal": "Cell and tissue research", "date": "2021", "authors": "Souza ATP, Lopes HB, Oliveira FS, Weffort D, Freitas GP, Adolpho LF, Fernandes RR, Rosa AL, Beloti MM", "evidence": [ { "database": "RefSeq", "id": "NP_067617", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_067617" } ], "reference": [ { "type": "PubMed", "id": "34223979", "url": "https://glygen.org/publication/PubMed/34223979" } ] }, { "title": "Agrin acts via a MuSK receptor complex.", "journal": "Cell", "date": "1996", "authors": "Shah S., Burden S.J., Bruno J., Radziejewski C., Ryan T.E., Valenzuela D.M., Yancopoulos G.D., DiStefano P.S., DeChiara T.M., Glass D.J., Stitt T.N., Gies D.R., Mattsson K., Bowen D.C.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "8653787", "url": "https://glygen.org/publication/PubMed/8653787" } ] }, { "title": "Selective regulation of agrin mRNA induction and alternative splicing in PC12 cells by Ras-dependent actions of nerve growth factor.", "journal": "J. Biol. Chem.", "date": "1997", "authors": "Smith M.A., Fanger G.R., Bridle P., Maue R.A., O'Connor L.T.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "reference": [ { "type": "PubMed", "id": "9188458", "url": "https://glygen.org/publication/PubMed/9188458" } ] } ], "pathway": [ { "id": "R-MMU-1971475", "name": "A tetrasaccharide linker sequence is required for GAG synthesis", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-1971475", "resource": "Reactome", "description": "A tetrasaccharide linker sequence is required for GAG synthesis" }, { "id": "R-MMU-2022928", "name": "HS-GAG biosynthesis", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-2022928", "resource": "Reactome", "description": "HS-GAG biosynthesis" }, { "id": "R-MMU-2024096", "name": "HS-GAG degradation", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-2024096", "resource": "Reactome", "description": "HS-GAG degradation" }, { "id": "R-MMU-3000178", "name": "ECM proteoglycans", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-3000178", "resource": "Reactome", "description": "ECM proteoglycans" }, { "id": "R-MMU-975634", "name": "Retinoid metabolism and transport", "url": "https://www.reactome.org/PathwayBrowser/#R-MMU-975634", "resource": "Reactome", "description": "Retinoid metabolism and transport" } ], "orthologs": [ { "uniprot_canonical_ac": "O00468-1", "protein_name": "Agrin", "gene_name": "AGRN", "tax_id": 9606, "organism": "Homo sapiens", "sequence": { "sequence": "MAGRSHPGPLRPLLPLLVVAACVLPGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESELANEIPVPETLDSGALHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP", "length": 2068 }, "evidence": [ { "id": "27907", "url": "http://www.informatics.jax.org/homology/27907", "database": "MGI" } ], "common_name": "Human" }, { "uniprot_canonical_ac": "P25304-1", "protein_name": "Agrin", "gene_name": "Agrn", "tax_id": 10116, "organism": "Rattus norvegicus", "sequence": { "sequence": "MPPLPLEHRPRQEPGASMLVRYFMIPCNICLILLATSTLGFAVLLFLSNYKPGIHFTPAPPTPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNACPATVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQEHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGEKVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSYSGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPMTPVTCVEIHCEFGASCVEKAGFAQCICPTLTCPEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVTPGASPTSASMTTPRHILSKTLPFPHNSLPLSPGSTTHDWPTPLPISPHTTVSIPRSTAWPVLTVPPTAAASDVTSLATSIFSESGSANGSGDEELSGDEEASGGGSGGLEPPVGSIVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFWSVRLRELGPGKLVRAIVDVHFDPTTAFQASDVGQALLRQIQVSRPWALAVRRPLQEHVRFLDFDWFPTFFTGAATGTTAAMATARATTVSRLPASSVTPRVYPSHTSRPVGRTTAPPTTRRPPTTATNMDRPRTPGHQQPSKSCDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCEKVQPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARGAAVSSGFSGVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGRTYIEYLNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTP", "length": 1959 }, "evidence": [ { "id": "27907", "url": "http://www.informatics.jax.org/homology/27907", "database": "MGI" } ], "common_name": "Rat" } ], "go_annotation": { "categories": [ { "name": "Cellular Component", "total": 13, "go_terms": [ { "name": "glutamatergic synapse", "id": "GO:0098978", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0098978", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "extracellular region", "id": "GO:0005576", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005576", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "extracellular space", "id": "GO:0005615", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005615", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "9422725" }, { "name": "Golgi lumen", "id": "GO:0005796", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005796", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "cell surface", "id": "GO:0009986", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0009986", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "19376779" }, { "name": "integral component of membrane", "id": "GO:0016021", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016021", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "synapse", "id": "GO:0045202", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045202", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "18230682" }, { "name": "synaptic cleft", "id": "GO:0043083", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043083", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "23056392" }, { "name": "sarcolemma", "id": "GO:0042383", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042383", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "19376779" }, { "name": "basement membrane", "id": "GO:0005604", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005604", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "7670489" }, { "name": "axonal growth cone", "id": "GO:0044295", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0044295", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "collagen-containing extracellular matrix", "id": "GO:0062023", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0062023", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "22159717" }, { "name": "plasma membrane", "id": "GO:0005886", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005886", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" } ] }, { "name": "Biological Process", "total": 30, "go_terms": [ { "name": "regulation of axon guidance", "id": "GO:1902667", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:1902667", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "neurotransmitter receptor metabolic process", "id": "GO:0045213", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045213", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "14622576" }, { "name": "positive regulation of protein phosphorylation", "id": "GO:0001934", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0001934", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "14622576" }, { "name": "plasma membrane organization", "id": "GO:0007009", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0007009", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "8653788" }, { "name": "neuromuscular junction development", "id": "GO:0007528", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0007528", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "12165471" }, { "name": "cell differentiation", "id": "GO:0030154", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030154", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "positive regulation of protein binding", "id": "GO:0032092", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0032092", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "21969364" }, { "name": "regulation of GTPase activity", "id": "GO:0043087", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043087", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "14622576" }, { "name": "positive regulation of GTPase activity", "id": "GO:0043547", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043547", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "positive regulation of transcription by RNA polymerase II", "id": "GO:0045944", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045944", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "9422725" }, { "name": "positive regulation of protein tyrosine kinase activity", "id": "GO:0061098", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0061098", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "19376779" }, { "name": "chemical synaptic transmission", "id": "GO:0007268", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0007268", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "8653788" }, { "name": "synapse assembly", "id": "GO:0007416", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0007416", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "animal organ morphogenesis", "id": "GO:0009887", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0009887", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "21873635" }, { "name": "positive regulation of peptidyl-tyrosine phosphorylation", "id": "GO:0050731", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050731", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "positive regulation of neuron apoptotic process", "id": "GO:0043525", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043525", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "11312299" }, { "name": "regulation of cardiac muscle contraction", "id": "GO:0055117", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0055117", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "19376779" }, { "name": "regulation of protein phosphorylation", "id": "GO:0001932", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0001932", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "receptor clustering", "id": "GO:0043113", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043113", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "17119023" }, { "name": "positive regulation of synaptic assembly at neuromuscular junction", "id": "GO:0045887", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0045887", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "10402191" }, { "name": "positive regulation of filopodium assembly", "id": "GO:0051491", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0051491", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "tissue development", "id": "GO:0009888", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0009888", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "21873635" }, { "name": "regulation of cardiac muscle cell membrane potential", "id": "GO:0086036", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0086036", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "19376779" }, { "name": "regulation of microtubule cytoskeleton organization", "id": "GO:0070507", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0070507", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "regulation of synapse organization", "id": "GO:0050807", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050807", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "positive regulation of protein geranylgeranylation", "id": "GO:2000541", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:2000541", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "14622576" }, { "name": "negative regulation of sodium ion export across plasma membrane", "id": "GO:1903277", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:1903277", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "19376779" }, { "name": "negative regulation of P-type sodium:potassium-exchanging transporter activity", "id": "GO:1903407", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:1903407", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "19376779" }, { "name": "skeletal muscle acetylcholine-gated channel clustering", "id": "GO:0071340", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0071340", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "G protein-coupled acetylcholine receptor signaling pathway", "id": "GO:0007213", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0007213", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" } ] }, { "name": "Molecular Function", "total": 11, "go_terms": [ { "name": "acetylcholine receptor regulator activity", "id": "GO:0030548", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030548", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "14622576" }, { "name": "heparan sulfate proteoglycan binding", "id": "GO:0043395", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043395", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "12773545" }, { "name": "transmembrane transporter binding", "id": "GO:0044325", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0044325", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "19376779" }, { "name": "calcium ion binding", "id": "GO:0005509", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005509", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "dystroglycan binding", "id": "GO:0002162", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0002162", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "transforming growth factor beta binding", "id": "GO:0050431", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0050431", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "sialic acid binding", "id": "GO:0033691", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0033691", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "chondroitin sulfate binding", "id": "GO:0035374", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0035374", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "12773545" }, { "name": "ATPase inhibitor activity", "id": "GO:0042030", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042030", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "19376779" }, { "name": "BMP binding", "id": "GO:0036122", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0036122", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" }, { "name": "laminin binding", "id": "GO:0043236", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043236", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" } ], "pmid": "" } ] } ] }, "pro_annotation": [], "site_annotation": [ { "start_pos": 145, "end_pos": 147, "annotation": "n_glycosylation_sequon" }, { "start_pos": 672, "end_pos": 674, "annotation": "n_glycosylation_sequon" }, { "start_pos": 827, "end_pos": 829, "annotation": "n_glycosylation_sequon" }, { "start_pos": 948, "end_pos": 950, "annotation": "n_glycosylation_sequon" } ], "ptm_annotation": [ { "annotation": "At synaptic junctions, cleaved at two conserved sites, alpha and beta, by neurotrypsin. Cleavage at the alpha-site produces the agrin N-terminal 110-kDa subunit and the agrin C-terminal 110-kDa subunit. Further cleavage of agrin C-terminal 110-kDa subunit at the beta site produces the C-terminal fragments, agrin C-terminal 90 kDa fragment and agrin C-terminal 22 kDa fragment. Excessive cleavage at the beta-site releases large amounts of the agrin C-terminal 22 kDa fragment leading to destabilization at the neuromuscular junction (NMJ). Cleavage is developmentally regulated. In developing brain, neurotrypsin-mediated cleavage occurs mainly during late fetal days and in the first postnatal week.", "evidence": [ { "database": "UniProtKB", "id": "A2ASQ1", "url": "https://www.uniprot.org/uniprot/A2ASQ1" }, { "database": "PubMed", "id": "18230682", "url": "https://glygen.org/publication/PubMed/18230682" }, { "database": "PubMed", "id": "21885656", "url": "https://glygen.org/publication/PubMed/21885656" }, { "database": "PubMed", "id": "19303856", "url": "https://glygen.org/publication/PubMed/19303856" } ] } ], "interactions": [], "reactions": [], "reaction_participants": [], "reaction_enzymes": [], "synthesized_glycans": [], "cluster_types": [ { "name": "isoformset.uniprotkb", "label": "Isoforms" }, { "name": "homologset.mgi", "label": "MGI Homologs" } ] }