{ "uniprot_canonical_ac": "A2AE42-1", "uniprot_ac": "A2AE42", "uniprot_id": "C56D1_MOUSE", "mass": { "chemical_mass": 25519.0, "monoisotopic_mass": 25519.0 }, "refseq": { "ac": "NP_001074789", "name": "probable transmembrane reductase CYB561D1 isoform 1", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001074789" }, "gene_names": [ { "name": "Cyb561d1", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/A2AE42", "id": "A2AE42" }, { "name": "1600010M23Rik; AI605489; AW457162", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_082337", "id": "NP_082337" }, { "name": "Cyb561d1", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_082337", "id": "NP_082337" } ], "protein_names": [ { "name": "Probable transmembrane reductase CYB561D1", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/A2AE42#names_and_taxonomy", "id": "A2AE42" }, { "name": "7.2.1.3", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/A2AE42#names_and_taxonomy", "id": "A2AE42" }, { "name": "Cytochrome b561 domain-containing protein 1", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/A2AE42#names_and_taxonomy", "id": "A2AE42" }, { "name": "cytochrome b561 domain-containing protein 1; probable transmembrane reductase CYB561D1", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001074789", "id": "NP_001074789" }, { "name": "probable transmembrane reductase CYB561D1 isoform 1", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001074789", "id": "NP_001074789" }, { "name": "probable transmembrane reductase CYB561D1 isoform 2", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_082337", "id": "NP_082337" } ], "function": [ { "annotation": "Probable transmembrane reductase that may use ascorbate as an electron donor and transfer electrons across membranes to reduce monodehydro-L-ascorbate radical and iron cations Fe(3+) in another cellular compartment.", "evidence": [ { "database": "UniProtKB", "id": "A2AE42", "url": "https://www.uniprot.org/uniprot/A2AE42#function" } ] } ], "disease": [], "snv": [], "mutagenesis": [], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Mus musculus", "taxid": 10090, "evidence": [ { "database": "UniProtKB", "id": "A2AE42", "url": "https://www.uniprot.org/uniprot/A2AE42" } ], "common_name": "Mouse" } ], "gene": [ { "name": "Cyb561d1", "url": "https://www.uniprot.org/uniprot/A2AE42", "locus": { "chromosome": "3", "start_pos": 108201212, "end_pos": 108195687, "strand": "-", "evidence": [ { "database": "Ensembl Gene", "id": "ENSMUSG00000048796", "url": "https://www.ensembl.org/id/ENSMUSG00000048796" } ] } } ], "crossref": [ { "id": "ENSMUSG00000048796", "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000048796", "database": "Bgee" }, { "id": "72023", "url": "https://www.ncbi.nlm.nih.gov/gene/72023", "database": "GeneID" }, { "id": "IPR006593", "url": "https://www.ebi.ac.uk/interpro/entry/IPR006593", "database": "InterPro" }, { "id": "mmu:72023", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:72023", "database": "KEGG Pathway" }, { "id": "1919273", "url": "http://www.informatics.jax.org/marker/MGI:1919273", "database": "MGI" }, { "id": "GSPFCFK", "url": "https://omabrowser.org/oma/group/GSPFCFK/", "database": "OMA" }, { "id": "1586923at2759", "url": "http://www.orthodb.org/?query=1586923at2759", "database": "OrthoDB" }, { "id": "PF03188", "url": "http://pfam.xfam.org/family/PF03188", "database": "Pfam" }, { "id": "PR_A2AE42", "url": "http://purl.obolibrary.org/obo/PR_A2AE42", "database": "PRO" }, { "id": "NP_001074789", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001074789", "database": "RefSeq" }, { "id": "NP_082337", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_082337", "database": "RefSeq" }, { "id": "30403", "url": "https://www.rhea-db.org/rhea/30403", "database": "Rhea" }, { "id": "66524", "url": "https://www.rhea-db.org/rhea/66524", "database": "Rhea" }, { "id": "A2AE42", "url": "https://www.uniprot.org/uniprot/A2AE42", "database": "UniProtKB" }, { "id": "A2AE42-1", "url": "https://www.uniprot.org/uniprot/A2AE42-1", "database": "UniProtKB" }, { "id": "Q3TPE5", "url": "https://www.uniprot.org/uniprot/Q3TPE5", "database": "UniProtKB" }, { "id": "Q3TPE5-1", "url": "https://www.uniprot.org/uniprot/Q3TPE5-1", "database": "UniProtKB" } ], "sequence": { "sequence": "MHSMEVGLVPAPAREPRLTRWLRRGSGILAHLIALGFTIFLTVLSRPGTSLFSWHPVFMALAFCLCMAEAILLFSPEHSLFFFCSRKTRIRLHWAGQTMAILCAVLGLGFIISSKIRSEMSHLVSWHSWIGALTLLATGGQALCGLCLLCPRAARVSRVARLKLYHLTCGLVVYLMATVTVLLGMYSVWFQAQIKGTAWYLCLGLPLYPALVIMHQISSSYLPRKKVEI", "length": 229, "header": "sp|A2AE42-1|C56D1_MOUSE Isoform 1 of Probable transmembrane reductase CYB561D1 OS=Mus musculus OX=10090 GN=Cyb561d1 PE=2 SV=1 CANONICAL=A2AE42-1" }, "isoforms": [ { "isoform_ac": "A2AE42-1", "url": "https://www.uniprot.org/uniprot/A2AE42#A2AE42-1", "sequence": { "sequence": "MHSMEVGLVPAPAREPRLTRWLRRGSGILAHLIALGFTIFLTVLSRPGTSLFSWHPVFMALAFCLCMAEAILLFSPEHSLFFFCSRKTRIRLHWAGQTMAILCAVLGLGFIISSKIRSEMSHLVSWHSWIGALTLLATGGQALCGLCLLCPRAARVSRVARLKLYHLTCGLVVYLMATVTVLLGMYSVWFQAQIKGTAWYLCLGLPLYPALVIMHQISSSYLPRKKVEI", "length": 229, "header": "sp|A2AE42-1|C56D1_MOUSE Isoform 1 of Probable transmembrane reductase CYB561D1 OS=Mus musculus OX=10090 GN=Cyb561d1 PE=2 SV=1 CANONICAL=A2AE42-1" }, "locus": { "chromosome": "3", "start_pos": 108108090, "end_pos": 108106531, "strand": "+", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000106655", "url": "https://www.ensembl.org/id/ENSMUST00000106655" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000102266", "url": "https://www.ensembl.org/id/ENSMUSP00000102266" } ] } }, { "isoform_ac": "Q3TPE5-1", "url": "https://www.uniprot.org/uniprot/A2AE42#Q3TPE5-1", "sequence": { "sequence": "MALAFCLCMAEAILLFSPEHSLFFFCSRKTRIRLHWAGQTMAILCAVLGLGFIISSKIRSEMSHLVSWHSWIGALTLLATGGQALCGLCLLCPRAARVSRVARLKLYHLTCGLVVYLMATVTVLLGMYSVWFQAQIKGTAWYLCLGLPLYPALVIMHQISSSYLPRKKVEI", "length": 171, "header": "tr|Q3TPE5-1|Q3TPE5_MOUSE Computationally mapped isoform of Cytochrome b561 domain-containing protein 1 OS=Mus musculus OX=10090 GN=Cyb561d1 PE=2 SV=1 CANONICAL=A2AE42-1" }, "locus": { "chromosome": "3", "start_pos": 108107452, "end_pos": 108106531, "strand": "+", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000065664", "url": "https://www.ensembl.org/id/ENSMUST00000065664" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000070361", "url": "https://www.ensembl.org/id/ENSMUSP00000070361" } ] } } ], "glycosylation": [], "phosphorylation": [], "glycation": [], "keywords": [ "enzyme" ], "publication": [], "pathway": [ { "id": "mmu:72023", "name": "", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:72023", "resource": "KEGG Pathway" } ], "orthologs": [ { "uniprot_canonical_ac": "A0A0G2K452-1", "protein_name": "Cytochrome b561 family, member D1", "gene_name": "Cyb561d1", "tax_id": 10116, "organism": "Rattus norvegicus", "sequence": { "sequence": "MHSMEVGLVPAPSREPRLTRWLRRGSGILAHLIALGFTIFLTVLSRPGTSLFSWHPVFMALAFCLCMAEAILLFSPEHSLFFFCSRKTRIRLHWAGQTMAILCAAVGLGFIISSKIRSELSHLVSWHSWMGALTLLATGGQALCGLGLLCPRAARVSRVARLKLYHMTCGLVVYLMATVTVLLGMYSVWFQAQIKGAAWYLCLVLPLYPALVIMHQISSSYLPRKKVDI", "length": 229 }, "evidence": [ { "id": "45651", "url": "http://www.informatics.jax.org/homology/45651", "database": "MGI" }, { "id": "A2AE42", "url": "https://omabrowser.org/oma/group/A2AE42/", "database": "OMA" } ], "common_name": "Rat" }, { "uniprot_canonical_ac": "Q8N8Q1-1", "protein_name": "Probable transmembrane reductase CYB561D1", "gene_name": "CYB561D1", "tax_id": 9606, "organism": "Homo sapiens", "sequence": { "sequence": "MQPLEVGLVPAPAGEPRLTRWLRRGSGILAHLVALGFTIFLTALSRPGTSLFSWHPVFMALAFCLCMAEAILLFSPEHSLFFFCSRKARIRLHWAGQTLAILCAALGLGFIISSRTRSELPHLVSWHSWVGALTLLATAVQALCGLCLLCPRAARVSRVARLKLYHLTCGLVVYLMATVTVLLGMYSVWFQAQIKGAAWYLCLALPVYPALVIMHQISRSYLPRKKMEM", "length": 229 }, "evidence": [ { "id": "45651", "url": "http://www.informatics.jax.org/homology/45651", "database": "MGI" } ], "common_name": "Human" } ], "go_annotation": { "categories": [ { "name": "Cellular Component", "total": 1, "go_terms": [ { "name": "integral component of membrane", "id": "GO:0016021", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016021", "evidence": [ { "database": "UniProtKB", "id": "A2AE42", "url": "https://www.uniprot.org/uniprot/A2AE42" } ], "pmid": "" } ] }, { "name": "Molecular Function", "total": 5, "go_terms": [ { "name": "metal ion binding", "id": "GO:0046872", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0046872", "evidence": [ { "database": "UniProtKB", "id": "A2AE42", "url": "https://www.uniprot.org/uniprot/A2AE42" } ], "pmid": "" }, { "name": "oxidoreductase activity", "id": "GO:0016491", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0016491", "evidence": [ { "database": "UniProtKB", "id": "A2AE42", "url": "https://www.uniprot.org/uniprot/A2AE42" } ], "pmid": "21873635" }, { "name": "heme binding", "id": "GO:0020037", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0020037", "evidence": [ { "database": "UniProtKB", "id": "A2AE42", "url": "https://www.uniprot.org/uniprot/A2AE42" } ], "pmid": "21873635" }, { "name": "transmembrane monodehydroascorbate reductase activity", "id": "GO:0140575", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0140575", "evidence": [ { "database": "UniProtKB", "id": "A2AE42", "url": "https://www.uniprot.org/uniprot/A2AE42" } ], "pmid": "" }, { "name": "transmembrane ascorbate ferrireductase activity", "id": "GO:0140571", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0140571", "evidence": [ { "database": "UniProtKB", "id": "A2AE42", "url": "https://www.uniprot.org/uniprot/A2AE42" } ], "pmid": "" } ] } ] }, "pro_annotation": [], "site_annotation": [], "ptm_annotation": [], "interactions": [], "reactions": [ { "id": "30403", "name": "", "equation": "Fe(3+)(out) + L-ascorbate(in) = Fe(2+)(out) + H(+) + monodehydro-L-ascorbate radical(in)", "summary": "", "cellular_location": "", "input_participant_list": [], "output_participant_list": [], "enzyme_list": [ "Q641Y1-1", "Q5U2W7-1", "Q5RKJ2-1", "Q8N8Q1-1", "Q53TN4-1", "Q8NBI2-1", "O14569-1", "Q925G2-1", "A2AE42-1", "Q9WUE3-1", "Q6P1H1-1" ], "evidence": [ { "id": "30403", "database": "Rhea", "url": "https://www.rhea-db.org/rhea/30403" } ] }, { "id": "66524", "name": "", "equation": "L-ascorbate(in) + monodehydro-L-ascorbate radical(out) = L-ascorbate(out) + monodehydro-L-ascorbate radical(in)", "summary": "", "cellular_location": "", "input_participant_list": [], "output_participant_list": [], "enzyme_list": [ "Q641Y1-1", "Q5RKJ2-1", "P49447-1", "Q8N8Q1-1", "Q53TN4-1", "O14569-1", "Q60720-1", "Q925G2-1", "A2AE42-1", "Q9WUE3-1" ], "evidence": [ { "id": "66524", "database": "Rhea", "url": "https://www.rhea-db.org/rhea/66524" } ] } ], "reaction_participants": [], "reaction_enzymes": [ { "uniprot_ac": "A2AE42", "gene_name": "Cyb561d1", "activity": [ { "ec_number": "7.2.1.3", "activity": "Ascorbate(Side 1) + Fe(III)(Side 2) = monodehydroascorbate(Side 1) + Fe(II)(Side 2).", "evidence": [ { "id": "A2AE42", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/A2AE42" } ] }, { "ec_number": "7.2.1.3", "activity": "", "evidence": [ { "id": "A2AE42", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/A2AE42" } ] } ] }, { "uniprot_ac": "O14569", "gene_name": "CYB561D2", "activity": [ { "ec_number": "7.2.1.3", "activity": "Ascorbate(Side 1) + Fe(III)(Side 2) = monodehydroascorbate(Side 1) + Fe(II)(Side 2).", "evidence": [ { "id": "O14569", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/O14569" } ] }, { "ec_number": "7.2.1.3", "activity": "", "evidence": [ { "id": "O14569", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/O14569" } ] } ] }, { "uniprot_ac": "P49447", "gene_name": "CYB561", "activity": [ { "ec_number": "7.2.1.-", "activity": "", "evidence": [ { "id": "P49447", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/P49447" } ] } ] }, { "uniprot_ac": "Q53TN4", "gene_name": "CYBRD1", "activity": [ { "ec_number": "7.2.1.3", "activity": "Ascorbate(Side 1) + Fe(III)(Side 2) = monodehydroascorbate(Side 1) + Fe(II)(Side 2).", "evidence": [ { "id": "Q53TN4", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q53TN4" } ] }, { "ec_number": "7.2.1.3", "activity": "", "evidence": [ { "id": "Q53TN4", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q53TN4" } ] } ] }, { "uniprot_ac": "Q5RKJ2", "gene_name": "Cybrd1", "activity": [ { "ec_number": "7.2.1.3", "activity": "Ascorbate(Side 1) + Fe(III)(Side 2) = monodehydroascorbate(Side 1) + Fe(II)(Side 2).", "evidence": [ { "id": "Q5RKJ2", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q5RKJ2" } ] }, { "ec_number": "7.2.1.3", "activity": "", "evidence": [ { "id": "Q5RKJ2", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q5RKJ2" } ] } ] }, { "uniprot_ac": "Q5U2W7", "gene_name": "Cyb561a3", "activity": [ { "ec_number": "7.2.1.3", "activity": "Ascorbate(Side 1) + Fe(III)(Side 2) = monodehydroascorbate(Side 1) + Fe(II)(Side 2).", "evidence": [ { "id": "Q5U2W7", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q5U2W7" } ] }, { "ec_number": "7.2.1.3", "activity": "", "evidence": [ { "id": "Q5U2W7", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q5U2W7" } ] } ] }, { "uniprot_ac": "Q60720", "gene_name": "Cyb561", "activity": [ { "ec_number": "7.2.1.-", "activity": "", "evidence": [ { "id": "Q60720", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q60720" } ] } ] }, { "uniprot_ac": "Q641Y1", "gene_name": "Cyb561d2", "activity": [ { "ec_number": "7.2.1.3", "activity": "Ascorbate(Side 1) + Fe(III)(Side 2) = monodehydroascorbate(Side 1) + Fe(II)(Side 2).", "evidence": [ { "id": "Q641Y1", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q641Y1" } ] }, { "ec_number": "7.2.1.3", "activity": "", "evidence": [ { "id": "Q641Y1", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q641Y1" } ] } ] }, { "uniprot_ac": "Q6P1H1", "gene_name": "Cyb561a3", "activity": [ { "ec_number": "7.2.1.3", "activity": "Ascorbate(Side 1) + Fe(III)(Side 2) = monodehydroascorbate(Side 1) + Fe(II)(Side 2).", "evidence": [ { "id": "Q6P1H1", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q6P1H1" } ] }, { "ec_number": "7.2.1.3", "activity": "", "evidence": [ { "id": "Q6P1H1", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q6P1H1" } ] } ] }, { "uniprot_ac": "Q8N8Q1", "gene_name": "CYB561D1", "activity": [ { "ec_number": "7.2.1.3", "activity": "Ascorbate(Side 1) + Fe(III)(Side 2) = monodehydroascorbate(Side 1) + Fe(II)(Side 2).", "evidence": [ { "id": "Q8N8Q1", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q8N8Q1" } ] }, { "ec_number": "7.2.1.3", "activity": "", "evidence": [ { "id": "Q8N8Q1", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q8N8Q1" } ] } ] }, { "uniprot_ac": "Q8NBI2", "gene_name": "CYB561A3", "activity": [ { "ec_number": "7.2.1.3", "activity": "Ascorbate(Side 1) + Fe(III)(Side 2) = monodehydroascorbate(Side 1) + Fe(II)(Side 2).", "evidence": [ { "id": "Q8NBI2", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q8NBI2" } ] }, { "ec_number": "7.2.1.3", "activity": "", "evidence": [ { "id": "Q8NBI2", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q8NBI2" } ] } ] }, { "uniprot_ac": "Q925G2", "gene_name": "Cybrd1", "activity": [ { "ec_number": "7.2.1.3", "activity": "Ascorbate(Side 1) + Fe(III)(Side 2) = monodehydroascorbate(Side 1) + Fe(II)(Side 2).", "evidence": [ { "id": "Q925G2", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q925G2" } ] }, { "ec_number": "7.2.1.3", "activity": "", "evidence": [ { "id": "Q925G2", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q925G2" } ] } ] }, { "uniprot_ac": "Q9WUE3", "gene_name": "Cyb561d2", "activity": [ { "ec_number": "7.2.1.3", "activity": "Ascorbate(Side 1) + Fe(III)(Side 2) = monodehydroascorbate(Side 1) + Fe(II)(Side 2).", "evidence": [ { "id": "Q9WUE3", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q9WUE3" } ] }, { "ec_number": "7.2.1.3", "activity": "", "evidence": [ { "id": "Q9WUE3", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/Q9WUE3" } ] } ] } ], "synthesized_glycans": [], "cluster_types": [ { "name": "isoformset.uniprotkb", "label": "Isoforms" }, { "name": "homologset.mgi", "label": "MGI Homologs" }, { "name": "homologset.oma", "label": "OMA Homologs" } ] }