{
    "uniprot_canonical_ac": "A2ADA5-1",
    "uniprot_ac": "A2ADA5",
    "uniprot_id": "PUSL1_MOUSE",
    "mass": {
        "chemical_mass": 31993.0,
        "monoisotopic_mass": 31993.0
    },
    "refseq": {
        "ac": "NP_001028662",
        "name": "tRNA pseudouridine synthase-like 1",
        "summary": "",
        "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028662"
    },
    "gene_names": [
        {
            "name": "Pusl1",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "https://www.uniprot.org/uniprot/A2ADA5",
            "id": "A2ADA5"
        },
        {
            "name": "2810021I11Rik; AI853843; D18346",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028662",
            "id": "NP_001028662"
        },
        {
            "name": "Pusl1",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028662",
            "id": "NP_001028662"
        }
    ],
    "protein_names": [
        {
            "name": "tRNA pseudouridine synthase-like 1",
            "resource": "UniProtKB",
            "type": "recommended",
            "url": "https://www.uniprot.org/uniprot/A2ADA5#names_and_taxonomy",
            "id": "A2ADA5"
        },
        {
            "name": "5.4.99.-",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "https://www.uniprot.org/uniprot/A2ADA5#names_and_taxonomy",
            "id": "A2ADA5"
        },
        {
            "name": "tRNA pseudouridine synthase-like 1",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028662",
            "id": "NP_001028662"
        },
        {
            "name": "tRNA pseudouridylate synthase-like 1",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "https://www.uniprot.org/uniprot/A2ADA5#names_and_taxonomy",
            "id": "A2ADA5"
        },
        {
            "name": "tRNA-uridine isomerase-like 1; tRNA pseudouridylate synthase-like 1",
            "resource": "RefSeq",
            "type": "synonym",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028662",
            "id": "NP_001028662"
        },
        {
            "name": "tRNA-uridine isomerase-like 1",
            "resource": "UniProtKB",
            "type": "synonym",
            "url": "https://www.uniprot.org/uniprot/A2ADA5#names_and_taxonomy",
            "id": "A2ADA5"
        }
    ],
    "function": [
        {
            "annotation": "MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001028662",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028662"
                },
                {
                    "database": "PubMed",
                    "id": "31693908",
                    "url": "https://glygen.org/publication/PubMed/31693908"
                }
            ]
        }
    ],
    "disease": [],
    "snv": [],
    "mutagenesis": [],
    "expression_tissue": [],
    "expression_disease": [],
    "species": [
        {
            "name": "Mus musculus",
            "taxid": 10090,
            "evidence": [
                {
                    "database": "UniProtKB",
                    "id": "A2ADA5",
                    "url": "https://www.uniprot.org/uniprot/A2ADA5"
                }
            ],
            "common_name": "Mouse"
        }
    ],
    "gene": [
        {
            "name": "Pusl1",
            "url": "https://www.uniprot.org/uniprot/A2ADA5",
            "locus": {
                "chromosome": "4",
                "start_pos": 155891781,
                "end_pos": 155887879,
                "strand": "-",
                "evidence": [
                    {
                        "database": "Ensembl Gene",
                        "id": "ENSMUSG00000051557",
                        "url": "https://www.ensembl.org/id/ENSMUSG00000051557"
                    }
                ]
            }
        }
    ],
    "crossref": [
        {
            "id": "ENSMUSG00000051557",
            "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000051557",
            "database": "Bgee"
        },
        {
            "id": "cd02570",
            "url": "https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd02570",
            "database": "CDD"
        },
        {
            "id": "433813",
            "url": "https://www.ncbi.nlm.nih.gov/gene/433813",
            "database": "GeneID"
        },
        {
            "id": "IPR001406",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR001406",
            "database": "InterPro"
        },
        {
            "id": "IPR020094",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR020094",
            "database": "InterPro"
        },
        {
            "id": "IPR020095",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR020095",
            "database": "InterPro"
        },
        {
            "id": "IPR020097",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR020097",
            "database": "InterPro"
        },
        {
            "id": "IPR020103",
            "url": "https://www.ebi.ac.uk/interpro/entry/IPR020103",
            "database": "InterPro"
        },
        {
            "id": "mmu:433813",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:433813",
            "database": "KEGG Pathway"
        },
        {
            "id": "3047787",
            "url": "http://www.informatics.jax.org/marker/MGI:3047787",
            "database": "MGI"
        },
        {
            "id": "FLYGMVR",
            "url": "https://omabrowser.org/oma/group/FLYGMVR/",
            "database": "OMA"
        },
        {
            "id": "831280at2759",
            "url": "http://www.orthodb.org/?query=831280at2759",
            "database": "OrthoDB"
        },
        {
            "id": "PTHR11142",
            "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR11142",
            "database": "PANTHER"
        },
        {
            "id": "PF01416",
            "url": "http://pfam.xfam.org/family/PF01416",
            "database": "Pfam"
        },
        {
            "id": "PR_A2ADA5",
            "url": "http://purl.obolibrary.org/obo/PR_A2ADA5",
            "database": "PRO"
        },
        {
            "id": "NP_001028662",
            "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028662",
            "database": "RefSeq"
        },
        {
            "id": "54572",
            "url": "https://www.rhea-db.org/rhea/54572",
            "database": "Rhea"
        },
        {
            "id": "A2ADA5",
            "url": "https://www.uniprot.org/uniprot/A2ADA5",
            "database": "UniProtKB"
        },
        {
            "id": "A2ADA5-1",
            "url": "https://www.uniprot.org/uniprot/A2ADA5-1",
            "database": "UniProtKB"
        },
        {
            "id": "A2ADA5-2",
            "url": "https://www.uniprot.org/uniprot/A2ADA5-2",
            "database": "UniProtKB"
        },
        {
            "id": "A2ADA5-3",
            "url": "https://www.uniprot.org/uniprot/A2ADA5-3",
            "database": "UniProtKB"
        }
    ],
    "sequence": {
        "sequence": "MGSCGAVGSVRARYLVFLQYLGTDFNGVAAVRGNPRAVGVLNFLEEAAKRLNSVDPVRFTISSRTDAGVHALSNAAHLDIQRRPGQSPFSPEVVAKALNTHLKHPAIRVLKAFRVPNDFHARHAATSRTYQYRLATGCSWPNQLPVFEQNVCWALQTEYLDMAAMQEAAQHLLGTHDFSAFQSAGSPVTNTVRTLRRVSVSPGPASPFVLPEGSRRLQFWTLEFESQSFLYRQVRRMTAVLVAVGLGILAPTQVKVILESQDPLGKYQARVAPARGLFLKSVLYDNFGPTS",
        "length": 291,
        "header": "sp|A2ADA5-1|PUSL1_MOUSE Isoform 1 of tRNA pseudouridine synthase-like 1  OS=Mus musculus OX=10090 GN=Pusl1 PE=1 SV=1 CANONICAL=A2ADA5-1"
    },
    "isoforms": [
        {
            "isoform_ac": "A2ADA5-1",
            "url": "https://www.uniprot.org/uniprot/A2ADA5#A2ADA5-1",
            "sequence": {
                "sequence": "MGSCGAVGSVRARYLVFLQYLGTDFNGVAAVRGNPRAVGVLNFLEEAAKRLNSVDPVRFTISSRTDAGVHALSNAAHLDIQRRPGQSPFSPEVVAKALNTHLKHPAIRVLKAFRVPNDFHARHAATSRTYQYRLATGCSWPNQLPVFEQNVCWALQTEYLDMAAMQEAAQHLLGTHDFSAFQSAGSPVTNTVRTLRRVSVSPGPASPFVLPEGSRRLQFWTLEFESQSFLYRQVRRMTAVLVAVGLGILAPTQVKVILESQDPLGKYQARVAPARGLFLKSVLYDNFGPTS",
                "length": 291,
                "header": "sp|A2ADA5-1|PUSL1_MOUSE Isoform 1 of tRNA pseudouridine synthase-like 1  OS=Mus musculus OX=10090 GN=Pusl1 PE=1 SV=1 CANONICAL=A2ADA5-1"
            },
            "locus": {
                "chromosome": "4",
                "start_pos": 155976164,
                "end_pos": 155973615,
                "strand": "+",
                "evidence": [
                    {
                        "database": "Ensembl Transcript",
                        "id": "ENSMUST00000097737",
                        "url": "https://www.ensembl.org/id/ENSMUST00000097737"
                    },
                    {
                        "database": "Ensembl Peptide",
                        "id": "ENSMUSP00000095344",
                        "url": "https://www.ensembl.org/id/ENSMUSP00000095344"
                    }
                ]
            }
        },
        {
            "isoform_ac": "A2ADA5-2",
            "url": "https://www.uniprot.org/uniprot/A2ADA5#A2ADA5-2",
            "sequence": {
                "sequence": "MWQPPVRFPQEAAKRLNSVDPVRFTISSRTDAGVHALSNAAHLDIQRRPGQSPFSPEVVAKALNTHLKHPAIRVLKAFRVPNDFHARHAATSRTYQYRLATGCSWPNQLPVFEQNVCWALQTEYLDMAAMQEAAQHLLGTHDFSAFQSAGSPVTNTVRTLRRVSVSPGPASPFVLPEGSRRLQFWTLEFESQSFLYRQVGCLNPHRMGSGRHTCDNLSSLLQVRRMTAVLVAVGLGILAPTQVKVILESQDPLGKYQARVAPARGLFLKSVLYDNFGPTS",
                "length": 280,
                "header": "sp|A2ADA5-2|PUSL1_MOUSE Isoform 2 of tRNA pseudouridine synthase-like 1  OS=Mus musculus OX=10090 GN=Pusl1 PE=1 SV=1 CANONICAL=A2ADA5-1"
            },
            "locus": {}
        },
        {
            "isoform_ac": "A2ADA5-3",
            "url": "https://www.uniprot.org/uniprot/A2ADA5#A2ADA5-3",
            "sequence": {
                "sequence": "MPGLHALSNAAHLDIQRRPGQSPFSPEVVAKALNTHLKHPAIRVLKAFRVPNDFHARHAATSRTYQYRLATGCSWPNQLPVFEQNVCWALQTEYLDMAAMQEAAQHLLGTHDFSAFQSAGSPVTNTVRTLRRVSVSPGPASPFVLPEGSRRLQFWTLEFESQSFLYRQVRRMTAVLVAVGLGILAPTQVKVILESQDPLGQVSGSSCSSPWPVLEVSAV",
                "length": 219,
                "header": "sp|A2ADA5-3|PUSL1_MOUSE Isoform 3 of tRNA pseudouridine synthase-like 1  OS=Mus musculus OX=10090 GN=Pusl1 PE=1 SV=1 CANONICAL=A2ADA5-1"
            },
            "locus": {}
        }
    ],
    "glycosylation": [],
    "phosphorylation": [],
    "glycation": [],
    "keywords": [
        "enzyme"
    ],
    "publication": [
        {
            "title": "MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.",
            "journal": "Cell reports",
            "date": "2019",
            "authors": "Busch JD, Cipullo M, Atanassov I, Bratic A, Silva Ramos E, Schondorf T, Li X, Pearce SF, Milenkovic D, Rorbach J, Larsson NG",
            "evidence": [
                {
                    "database": "RefSeq",
                    "id": "NP_001028662",
                    "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001028662"
                }
            ],
            "reference": [
                {
                    "type": "PubMed",
                    "id": "31693908",
                    "url": "https://glygen.org/publication/PubMed/31693908"
                }
            ]
        }
    ],
    "pathway": [
        {
            "id": "mmu:433813",
            "name": "",
            "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:433813",
            "resource": "KEGG Pathway"
        }
    ],
    "orthologs": [
        {
            "uniprot_canonical_ac": "A0A0G2K730-1",
            "protein_name": "tRNA pseudouridine synthase",
            "gene_name": "Pusl1",
            "tax_id": 10116,
            "organism": "Rattus norvegicus",
            "sequence": {
                "sequence": "MGSCGAVGSVRARYLVFFQYLGTDFNGVAAVRGNHRAVGVQNFLEEAAKRLNSVEPVRFTISSRTDAGVHALSNAAHLDIQRRPGRPPFSPEIVTKALNTHLKHPAIRVLKAFRVPNDFHARHAATSRTYLYRLATGCSGPNQLPVFEQNVCWSLQTEYLDIAAMQEAAQHLLGTHDFSAFQSAGSPVPHAVRTLRRVSVSPGPASLFVLPQESRRLQFWTLEFESQSFLYRQVRRMTAVLVAVGLGILAPTQVKVILESQDPLGKYQARVAPAHGLFLKSVLYDNFGPTS",
                "length": 291
            },
            "evidence": [
                {
                    "id": "13655",
                    "url": "http://www.informatics.jax.org/homology/13655",
                    "database": "MGI"
                },
                {
                    "id": "A2ADA5",
                    "url": "https://omabrowser.org/oma/group/A2ADA5/",
                    "database": "OMA"
                }
            ],
            "common_name": "Rat"
        },
        {
            "uniprot_canonical_ac": "Q8N0Z8-1",
            "protein_name": "tRNA pseudouridine synthase-like 1",
            "gene_name": "PUSL1",
            "tax_id": 9606,
            "organism": "Homo sapiens",
            "sequence": {
                "sequence": "MSSAPASGSVRARYLVYFQYVGTDFNGVAAVRGTQRAVGVQNYLEEAAERLNSVEPVRFTISSRTDAGVHALSNAAHLDVQRRSGRPPFPPEVLAEALNTHLRHPAIRVLRAFRVPSDFHARHAATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCLDMVAMQEAAQHLLGTHDFSAFQSAGSPVPSPVRTLRRVSVSPGQASPLVTPEESRKLRFWNLEFESQSFLYRQVRRMTAVLVAVGLGALAPAQVKTILESQDPLGKHQTRVAPAHGLFLKSVLYGNLGAASCTLQGPQFGSHG",
                "length": 303
            },
            "evidence": [
                {
                    "id": "13655",
                    "url": "http://www.informatics.jax.org/homology/13655",
                    "database": "MGI"
                },
                {
                    "id": "A2ADA5",
                    "url": "https://omabrowser.org/oma/group/A2ADA5/",
                    "database": "OMA"
                }
            ],
            "common_name": "Human"
        }
    ],
    "go_annotation": {
        "categories": [
            {
                "name": "Biological Process",
                "total": 1,
                "go_terms": [
                    {
                        "name": "tRNA pseudouridine synthesis",
                        "id": "GO:0031119",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0031119",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "A2ADA5",
                                "url": "https://www.uniprot.org/uniprot/A2ADA5"
                            }
                        ],
                        "pmid": "21873635"
                    }
                ]
            },
            {
                "name": "Molecular Function",
                "total": 2,
                "go_terms": [
                    {
                        "name": "RNA binding",
                        "id": "GO:0003723",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0003723",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "A2ADA5",
                                "url": "https://www.uniprot.org/uniprot/A2ADA5"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "pseudouridine synthase activity",
                        "id": "GO:0009982",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0009982",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "A2ADA5",
                                "url": "https://www.uniprot.org/uniprot/A2ADA5"
                            }
                        ],
                        "pmid": "21873635"
                    }
                ]
            },
            {
                "name": "Cellular Component",
                "total": 2,
                "go_terms": [
                    {
                        "name": "mitochondrion",
                        "id": "GO:0005739",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005739",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "A2ADA5",
                                "url": "https://www.uniprot.org/uniprot/A2ADA5"
                            }
                        ],
                        "pmid": ""
                    },
                    {
                        "name": "intracellular membrane-bounded organelle",
                        "id": "GO:0043231",
                        "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0043231",
                        "evidence": [
                            {
                                "database": "UniProtKB",
                                "id": "A2ADA5",
                                "url": "https://www.uniprot.org/uniprot/A2ADA5"
                            }
                        ],
                        "pmid": ""
                    }
                ]
            }
        ]
    },
    "pro_annotation": [],
    "site_annotation": [],
    "ptm_annotation": [],
    "interactions": [],
    "reactions": [
        {
            "id": "54572",
            "name": "",
            "equation": "a uridine in tRNA = a pseudouridine in tRNA",
            "summary": "",
            "cellular_location": "",
            "input_participant_list": [],
            "output_participant_list": [],
            "enzyme_list": [
                "Q4QQT0-1",
                "Q8N0Z8-1",
                "Q96PZ0-1",
                "Q96CM3-1",
                "A2ADA5-1",
                "Q9CWX4-1",
                "Q91VU7-1"
            ],
            "evidence": [
                {
                    "id": "54572",
                    "database": "Rhea",
                    "url": "https://www.rhea-db.org/rhea/54572"
                }
            ]
        }
    ],
    "reaction_participants": [],
    "reaction_enzymes": [
        {
            "uniprot_ac": "A2ADA5",
            "gene_name": "Pusl1",
            "activity": [
                {
                    "ec_number": "5.4.99.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "A2ADA5",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/A2ADA5"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q4QQT0",
            "gene_name": "Rpusd4",
            "activity": [
                {
                    "ec_number": "5.4.99.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q4QQT0",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q4QQT0"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q8N0Z8",
            "gene_name": "PUSL1",
            "activity": [
                {
                    "ec_number": "5.4.99.12",
                    "activity": "tRNA uridine(38-40) = tRNA pseudouridine(38-40).",
                    "evidence": [
                        {
                            "id": "Q8N0Z8",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q8N0Z8"
                        }
                    ]
                },
                {
                    "ec_number": "5.4.99.12",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q8N0Z8",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q8N0Z8"
                        }
                    ]
                },
                {
                    "ec_number": "5.4.99.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q8N0Z8",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q8N0Z8"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q91VU7",
            "gene_name": "Pus7",
            "activity": [
                {
                    "ec_number": "5.4.99.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q91VU7",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q91VU7"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q96CM3",
            "gene_name": "RPUSD4",
            "activity": [
                {
                    "ec_number": "5.4.99.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q96CM3",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q96CM3"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q96PZ0",
            "gene_name": "PUS7",
            "activity": [
                {
                    "ec_number": "5.4.99.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q96PZ0",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q96PZ0"
                        }
                    ]
                }
            ]
        },
        {
            "uniprot_ac": "Q9CWX4",
            "gene_name": "Rpusd4",
            "activity": [
                {
                    "ec_number": "5.4.99.-",
                    "activity": "",
                    "evidence": [
                        {
                            "id": "Q9CWX4",
                            "database": "UniProtKB",
                            "url": "https://www.uniprot.org/uniprot/Q9CWX4"
                        }
                    ]
                }
            ]
        }
    ],
    "synthesized_glycans": [],
    "cluster_types": [
        {
            "name": "isoformset.uniprotkb",
            "label": "Isoforms"
        },
        {
            "name": "homologset.mgi",
            "label": "MGI Homologs"
        },
        {
            "name": "homologset.oma",
            "label": "OMA Homologs"
        }
    ]
}