{ "uniprot_canonical_ac": "A2A6C4-1", "uniprot_ac": "A2A6C4", "uniprot_id": "SPP2C_MOUSE", "mass": { "chemical_mass": 76266.0, "monoisotopic_mass": 76266.0 }, "refseq": { "ac": "NP_001076004", "name": "signal peptide peptidase-like 2C isoform b precursor", "summary": "", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001076004" }, "gene_names": [ { "name": "Sppl2c", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/A2A6C4", "id": "A2A6C4" }, { "name": "4933407P14Rik; IMP5", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001076004", "id": "NP_001076004" }, { "name": "Imp5", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/A2A6C4", "id": "A2A6C4" }, { "name": "Sppl2c", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001076004", "id": "NP_001076004" } ], "protein_names": [ { "name": "Signal peptide peptidase-like 2C", "resource": "UniProtKB", "type": "recommended", "url": "https://www.uniprot.org/uniprot/A2A6C4#names_and_taxonomy", "id": "A2A6C4" }, { "name": "3.4.23.-", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/A2A6C4#names_and_taxonomy", "id": "A2A6C4" }, { "name": "IMP-5", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/A2A6C4#names_and_taxonomy", "id": "A2A6C4" }, { "name": "Intramembrane protease 5", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/A2A6C4#names_and_taxonomy", "id": "A2A6C4" }, { "name": "SPP-like 2C", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/A2A6C4#names_and_taxonomy", "id": "A2A6C4" }, { "name": "SPPL2c", "resource": "UniProtKB", "type": "synonym", "url": "https://www.uniprot.org/uniprot/A2A6C4#names_and_taxonomy", "id": "A2A6C4" }, { "name": "signal peptide peptidase-like 2C isoform b precursor", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001076004", "id": "NP_001076004" }, { "name": "signal peptide peptidase-like 2C; ortholog of human intramembrane protease 5 IMP5; IMP-5; protein SPPL2c; protein SPP-like 2C", "resource": "RefSeq", "type": "synonym", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001076004", "id": "NP_001076004" } ], "function": [ { "annotation": "Intramembrane-cleaving aspartic protease (I-CLiP) that may be able to cleave type II membrane signal peptides in the hydrophobic plane of the membrane.", "evidence": [ { "database": "UniProtKB", "id": "A2A6C4", "url": "https://www.uniprot.org/uniprot/A2A6C4#function" } ] }, { "annotation": "The sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA2)-regulating protein phospholamban (PLN) is identified as a physiological SPPL2c substrate.", "evidence": [ { "database": "RefSeq", "id": "NP_001076004", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001076004" }, { "database": "PubMed", "id": "30733280", "url": "https://glygen.org/publication/PubMed/30733280" } ] }, { "annotation": "These data suggest involvement of SPPL2c in acrosome formation during spermatogenesis. Erratum:[EMBO Rep. 2019 May;20(5):. PMID: 31061204]", "evidence": [ { "database": "RefSeq", "id": "NP_001076004", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001076004" }, { "database": "PubMed", "id": "30733281", "url": "https://glygen.org/publication/PubMed/30733281" } ] } ], "disease": [], "snv": [], "mutagenesis": [], "expression_tissue": [], "expression_disease": [], "species": [ { "name": "Mus musculus", "taxid": 10090, "evidence": [ { "database": "UniProtKB", "id": "A2A6C4", "url": "https://www.uniprot.org/uniprot/A2A6C4" } ], "common_name": "Mouse" } ], "gene": [ { "name": "Sppl2c", "url": "https://www.uniprot.org/uniprot/A2A6C4", "locus": { "chromosome": "11", "start_pos": 104186327, "end_pos": 104191163, "strand": "+", "evidence": [ { "database": "Ensembl Gene", "id": "ENSMUSG00000049506", "url": "https://www.ensembl.org/id/ENSMUSG00000049506" } ] } } ], "crossref": [ { "id": "ENSMUSG00000049506", "url": "https://bgee.org/?page=gene&gene_id=ENSMUSG00000049506", "database": "Bgee" }, { "id": "3.4.23.B24", "url": "https://www.brenda-enzymes.org/enzyme.php?ecno=3.4.23.B24&UniProtAcc=A2A6C4", "database": "BRENDA" }, { "id": "237958", "url": "https://www.ncbi.nlm.nih.gov/gene/237958", "database": "GeneID" }, { "id": "IPR006639", "url": "https://www.ebi.ac.uk/interpro/entry/IPR006639", "database": "InterPro" }, { "id": "IPR007369", "url": "https://www.ebi.ac.uk/interpro/entry/IPR007369", "database": "InterPro" }, { "id": "IPR033150", "url": "https://www.ebi.ac.uk/interpro/entry/IPR033150", "database": "InterPro" }, { "id": "mmu:237958", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:237958", "database": "KEGG Pathway" }, { "id": "3045264", "url": "http://www.informatics.jax.org/marker/MGI:3045264", "database": "MGI" }, { "id": "AQGEYGV", "url": "https://omabrowser.org/oma/group/AQGEYGV/", "database": "OMA" }, { "id": "535101at2759", "url": "http://www.orthodb.org/?query=535101at2759", "database": "OrthoDB" }, { "id": "PTHR12174", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR12174", "database": "PANTHER" }, { "id": "PTHR12174:SF38", "url": "http://www.pantherdb.org/panther/family.do?clsAccession=PTHR12174:SF38", "database": "PANTHER" }, { "id": "PF04258", "url": "http://pfam.xfam.org/family/PF04258", "database": "Pfam" }, { "id": "PR_A2A6C4", "url": "http://purl.obolibrary.org/obo/PR_A2A6C4", "database": "PRO" }, { "id": "NP_001076004", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001076004", "database": "RefSeq" }, { "id": "A2A6C4", "url": "https://www.uniprot.org/uniprot/A2A6C4", "database": "UniProtKB" }, { "id": "A2A6C4-1", "url": "https://www.uniprot.org/uniprot/A2A6C4-1", "database": "UniProtKB" }, { "id": "A2A6C4-2", "url": "https://www.uniprot.org/uniprot/A2A6C4-2", "database": "UniProtKB" } ], "sequence": { "sequence": "MACLGSLHPLGSLLLLFLLLLLSPEARGEYGLVRVVSKNWSKDYCVLYSSDYVNLPRDLHHAPLLSLHDGTKTPWCPDEDSFHQAQDSSPRQRPLHQTTTMVTRGNCSFYAKGWLAQDQGAQGLLIVSRARNQQCSDTISKPQDPSKPWPALTIPVAVLRYTDMLDIVSHTYGDTDVRVAMFAPLEPVTDYNMAIIFILAVGTVAAGGYWAGLMEANKLQRRQAQRGGGLGGHNQQQTVAAERSQRAWEDDDFEDAPMDFTPAMTGAVVTMSCSIMILLYFFYDCFVYVMIGIFSLGASTGLYSCLAPILCHLPLWRYQWVLPGQRVSVTWPLLLLAGLCAMVTVLWVIHRNEDHWAWLLQDTLGVAYCLFVLRRVRLPTFKNCTLFLLALLAFDVFFVFITPLFTKTGESIMVEVASGPADSSSHERLPMVLKVPRLSFSALTLCNQPFSILGFGDIVVPGFLVAYCHRFDMQVQSRQVYYMACTVAYAVGLLVTFVAMILMQMGQPALLYLVSSTLLTSLAVATCRQEFTLFWTGQGRAKIPAEPVAQPCIASAVGSKMKLEDAKDSRTTNRFEQAVDGESGDLESSTGDDMAEMVTLSEDEATSPEGHSESSEGWSDTNLDPNELPSGSPMALEAMLIPLIQPIPHPSELGHIRTQSRVHDSSLPWMGLHKRKGLKVKKSMSAQAPL", "length": 690, "header": "sp|A2A6C4-1|SPP2C_MOUSE Isoform 1 of Signal peptide peptidase-like 2C OS=Mus musculus OX=10090 GN=Sppl2c PE=2 SV=1 CANONICAL=A2A6C4-1" }, "isoforms": [ { "isoform_ac": "A2A6C4-1", "url": "https://www.uniprot.org/uniprot/A2A6C4#A2A6C4-1", "sequence": { "sequence": "MACLGSLHPLGSLLLLFLLLLLSPEARGEYGLVRVVSKNWSKDYCVLYSSDYVNLPRDLHHAPLLSLHDGTKTPWCPDEDSFHQAQDSSPRQRPLHQTTTMVTRGNCSFYAKGWLAQDQGAQGLLIVSRARNQQCSDTISKPQDPSKPWPALTIPVAVLRYTDMLDIVSHTYGDTDVRVAMFAPLEPVTDYNMAIIFILAVGTVAAGGYWAGLMEANKLQRRQAQRGGGLGGHNQQQTVAAERSQRAWEDDDFEDAPMDFTPAMTGAVVTMSCSIMILLYFFYDCFVYVMIGIFSLGASTGLYSCLAPILCHLPLWRYQWVLPGQRVSVTWPLLLLAGLCAMVTVLWVIHRNEDHWAWLLQDTLGVAYCLFVLRRVRLPTFKNCTLFLLALLAFDVFFVFITPLFTKTGESIMVEVASGPADSSSHERLPMVLKVPRLSFSALTLCNQPFSILGFGDIVVPGFLVAYCHRFDMQVQSRQVYYMACTVAYAVGLLVTFVAMILMQMGQPALLYLVSSTLLTSLAVATCRQEFTLFWTGQGRAKIPAEPVAQPCIASAVGSKMKLEDAKDSRTTNRFEQAVDGESGDLESSTGDDMAEMVTLSEDEATSPEGHSESSEGWSDTNLDPNELPSGSPMALEAMLIPLIQPIPHPSELGHIRTQSRVHDSSLPWMGLHKRKGLKVKKSMSAQAPL", "length": 690, "header": "sp|A2A6C4-1|SPP2C_MOUSE Isoform 1 of Signal peptide peptidase-like 2C OS=Mus musculus OX=10090 GN=Sppl2c PE=2 SV=1 CANONICAL=A2A6C4-1" }, "locus": { "chromosome": "11", "start_pos": 104079271, "end_pos": 104077202, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000107000", "url": "https://www.ensembl.org/id/ENSMUST00000107000" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000102613", "url": "https://www.ensembl.org/id/ENSMUSP00000102613" } ] } }, { "isoform_ac": "A2A6C4-2", "url": "https://www.uniprot.org/uniprot/A2A6C4#A2A6C4-2", "sequence": { "sequence": "MACLGSLHPLGSLLLLFLLLLLSPEARGEYGLVRVVSKNWSKDYCVLYSSDYVNLPRDLHHAPLLSLHDGTKTPWCPDEDSFHQAQDSSPRQRPLHQTTTMVTRGNCSFYAKGWLAQDQGAQGLLIVSRARNQQCSDTISKPQDPSKPWPALTIPVAVLRYTDMLDIVSHTYGDTDVRVAMFAPLEPVTDYNMAIIFILAVGTVAAGGYWAGLMEANKLQRRQAQRGGGLGGHNQQQTVAAERSQRAWEDDDFEDAPMDFTPAMTGAVVTMSCSIMILLYFFYDCFVYVMIGIFSLGASTGLYSCLAPILCHLPLWRYQWVLPGQRVSVTWPLLLLAGLCAMVTVLWVIHRNEDHWAWLLQDTLGVAYCLFVLRRVRLPTFKNCTLFLLALLAFDVFFVFITPLFTKTGESIMVEVASGPADSSSHERLPMVLKVPRLSFSALTLCNQPFSILGFGDIVVPGFLVAYCHRFDMQVQSRQVYYMACTVAYAVGLLVTFVAMILMQMGQPALLYLVSSTLLTSLAVATCRQEFTLFWTGQGRAKIPAEPVAQPCIASAVGSKMKLEDAKDSRTTNRMPEEDFV", "length": 581, "header": "sp|A2A6C4-2|SPP2C_MOUSE Isoform 2 of Signal peptide peptidase-like 2C OS=Mus musculus OX=10090 GN=Sppl2c PE=2 SV=1 CANONICAL=A2A6C4-1" }, "locus": { "chromosome": "11", "start_pos": 104081564, "end_pos": 104077202, "strand": "-", "evidence": [ { "database": "Ensembl Transcript", "id": "ENSMUST00000059448", "url": "https://www.ensembl.org/id/ENSMUST00000059448" }, { "database": "Ensembl Peptide", "id": "ENSMUSP00000091453", "url": "https://www.ensembl.org/id/ENSMUSP00000091453" } ] } } ], "glycosylation": [ { "glytoucan_ac": "", "type": "N-linked", "subtype": "", "site_category": "predicted", "site_seq": "N", "relation": "attached", "comment": "N-linked (GlcNAc...) asparagine", "start_pos": 106, "start_aa": "Asn", "end_pos": 106, "end_aa": "Asn", "residue": "Asn", "evidence": [ { "id": "A2A6C4", "database": "UniProtKB", "url": "https://www.uniprot.org/uniprot/A2A6C4#ptm_processing" } ] } ], "phosphorylation": [], "glycation": [], "keywords": [ "enzyme", "glycoprotein", "glycoprotein_predicted" ], "publication": [ { "title": "The intramembrane protease SPPL2c promotes male germ cell development by cleaving phospholamban.", "journal": "EMBO reports", "date": "2019", "authors": "Niemeyer J, Mentrup T, Heidasch R, Muller SA, Biswas U, Meyer R, Papadopoulou AA, Dederer V, Haug-Kroper M, Adamski V, Lullmann-Rauch R, Bergmann M, Mayerhofer A, Saftig P, Wennemuth G, Jessberger R, Fluhrer R, Lichtenthaler SF, Lemberg MK, Schroder B", "evidence": [ { "database": "RefSeq", "id": "NP_001076004", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001076004" } ], "reference": [ { "type": "PubMed", "id": "30733280", "url": "https://glygen.org/publication/PubMed/30733280" } ] }, { "title": "Signal Peptide Peptidase-Like 2c (SPPL2c) impairs vesicular transport and cleavage of SNARE proteins.", "journal": "EMBO reports", "date": "2019", "authors": "Papadopoulou AA, Muller SA, Mentrup T, Shmueli MD, Niemeyer J, Haug-Kroper M, von Blume J, Mayerhofer A, Feederle R, Schroder B, Lichtenthaler SF, Fluhrer R", "evidence": [ { "database": "RefSeq", "id": "NP_001076004", "url": "https://www.ncbi.nlm.nih.gov/protein/NP_001076004" } ], "reference": [ { "type": "PubMed", "id": "30733281", "url": "https://glygen.org/publication/PubMed/30733281" } ] } ], "pathway": [ { "id": "mmu:237958", "name": "", "url": "https://www.genome.jp/dbget-bin/get_linkdb?-t+pathway+mmu:237958", "resource": "KEGG Pathway" } ], "orthologs": [ { "uniprot_canonical_ac": "M0R9B3-1", "protein_name": "Signal peptide peptidase-like 2C", "gene_name": "Sppl2c", "tax_id": 10116, "organism": "Rattus norvegicus", "sequence": { "sequence": "MACLGSLLPIGSLLLLLLSPEARGEYGVVRVVSKNWNKDYCVLYSSDYVTLPRDLSHAPLLPLHDGTKTPWCPDEDSFHQAQDSSLSQRPLHQTTTMVVRGNCSFYAKGWLAQDHGAHGLLIVSRASNQQCSDTTSKPQDPSKPRPALTIPVAVLRYTDMLDILSHTYGDTNVHIAMYAPLEPIIDYNMVIIFILAVGTVAAGGYWAGLMEADRLQRHQARRGGGFGGHNQSQTVSAERSPRAWKEEDYEDAAVDFTPAMTGAVVTMSCSIMVLLYFFYDCFVYIMIGIFGLGASTGLYSCLAPIVRYLPLWQHQWVLPGHRASVKLSLLLLAGLCAMVTVLWVIYRNEDRWAWLLQDTLGVAYCLFVLRRVRLPTLKNCTSFLLALLAFDVFFVFITPLFTKTGESIMVEVASGPVDSSSHERLPMVLKVPRMSFSALTLCDQPFSILGFGDIVVPGFLVAYCHRFDVQIQSRQVYYRACTVAYAMGLLVTFVAMVLMQMGQPALLYLVSSTLLTSLVVATCRQELTLFWTGQGRAKIPAEPVVQPCIVSAVACRMKLEGVKDSRVTNRKTPAPGNQEASFKPQLTWLPEPSAQGPDISPCPCP", "length": 605 }, "evidence": [ { "id": "18491", "url": "http://www.informatics.jax.org/homology/18491", "database": "MGI" } ], "common_name": "Rat" }, { "uniprot_canonical_ac": "Q8IUH8-1", "protein_name": "Signal peptide peptidase-like 2C", "gene_name": "SPPL2C", "tax_id": 9606, "organism": "Homo sapiens", "sequence": { "sequence": "MACLGFLLPVGFLLLISTVAGGKYGVAHVVSENWSKDYCILFSSDYITLPRDLHHAPLLPLYDGTKAPWCPGEDSPHQAQLRSPSQRPLRQTTAMVMRGNCSFHTKGWLAQGQGAHGLLIVSRVSDQQCSDTTLAPQDPRQPLADLTIPVAMLHYADMLDILSHTRGEAVVRVAMYAPPEPIIDYNMLVIFILAVGTVAAGGYWAGLTEANRLQRRRARRGGGSGGHHQLQEAAAAEGAQKEDNEDIPVDFTPAMTGVVVTLSCSLMLLLYFFYDHFVYVTIGIFGLGAGIGLYSCLSPLVCRLSLRQYQRPPHSLWASLPLPLLLLASLCATVIIFWVAYRNEDRWAWLLQDTLGISYCLFVLHRVRLPTLKNCSSFLLALLAFDVFFVFVTPFFTKTGESIMAQVALGPAESSSHERLPMVLKVPRLRVSALTLCSQPFSILGFGDIVVPGFLVAYCCRFDVQVCSRQIYFVACTVAYAVGLLVTFMAMVLMQMGQPALLYLVSSTLLTSLAVAACRQELSLFWTGQGRAKMCGLGCAPSAGSRQKQEGAADAHTASTLERGTSRGAGDLDSNPGEDTTEIVTISENEATNPEDRSDSSEGWSDAHLDPNELPFIPPGASEELMPLMPMAMLIPLMPLMPPPSELGHVHAQAQAHETGLPWAGLHKRKGLKVRKSMSTQAPL", "length": 684 }, "evidence": [ { "id": "18491", "url": "http://www.informatics.jax.org/homology/18491", "database": "MGI" } ], "common_name": "Human" } ], "go_annotation": { "categories": [ { "name": "Cellular Component", "total": 5, "go_terms": [ { "name": "integral component of cytoplasmic side of endoplasmic reticulum membrane", "id": "GO:0071458", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0071458", "evidence": [ { "database": "UniProtKB", "id": "A2A6C4", "url": "https://www.uniprot.org/uniprot/A2A6C4" } ], "pmid": "" }, { "name": "lysosomal membrane", "id": "GO:0005765", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005765", "evidence": [ { "database": "UniProtKB", "id": "A2A6C4", "url": "https://www.uniprot.org/uniprot/A2A6C4" } ], "pmid": "21873635" }, { "name": "endoplasmic reticulum membrane", "id": "GO:0005789", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0005789", "evidence": [ { "database": "UniProtKB", "id": "A2A6C4", "url": "https://www.uniprot.org/uniprot/A2A6C4" } ], "pmid": "" }, { "name": "integral component of lumenal side of endoplasmic reticulum membrane", "id": "GO:0071556", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0071556", "evidence": [ { "database": "UniProtKB", "id": "A2A6C4", "url": "https://www.uniprot.org/uniprot/A2A6C4" } ], "pmid": "" }, { "name": "Golgi-associated vesicle membrane", "id": "GO:0030660", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0030660", "evidence": [ { "database": "UniProtKB", "id": "A2A6C4", "url": "https://www.uniprot.org/uniprot/A2A6C4" } ], "pmid": "21873635" } ] }, { "name": "Molecular Function", "total": 2, "go_terms": [ { "name": "protein homodimerization activity", "id": "GO:0042803", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042803", "evidence": [ { "database": "UniProtKB", "id": "A2A6C4", "url": "https://www.uniprot.org/uniprot/A2A6C4" } ], "pmid": "" }, { "name": "aspartic endopeptidase activity, intramembrane cleaving", "id": "GO:0042500", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0042500", "evidence": [ { "database": "UniProtKB", "id": "A2A6C4", "url": "https://www.uniprot.org/uniprot/A2A6C4" } ], "pmid": "21873635" } ] }, { "name": "Biological Process", "total": 1, "go_terms": [ { "name": "membrane protein proteolysis", "id": "GO:0033619", "url": "https://www.ebi.ac.uk/QuickGO/term/GO:0033619", "evidence": [ { "database": "UniProtKB", "id": "A2A6C4", "url": "https://www.uniprot.org/uniprot/A2A6C4" } ], "pmid": "21873635" } ] } ] }, "pro_annotation": [], "site_annotation": [ { "start_pos": 106, "end_pos": 108, "annotation": "n_glycosylation_sequon" }, { "start_pos": 383, "end_pos": 385, "annotation": "n_glycosylation_sequon" }, { "start_pos": 39, "end_pos": 41, "annotation": "n_glycosylation_sequon" } ], "ptm_annotation": [ { "annotation": "Glycosylated.", "evidence": [ { "database": "UniProtKB", "id": "A2A6C4", "url": "https://www.uniprot.org/uniprot/A2A6C4" }, { "database": "PubMed", "id": "P49768", "url": "https://glygen.org/publication/PubMed/P49768" } ] } ], "interactions": [], "reactions": [], "reaction_participants": [], "reaction_enzymes": [], "synthesized_glycans": [], "cluster_types": [ { "name": "isoformset.uniprotkb", "label": "Isoforms" }, { "name": "homologset.mgi", "label": "MGI Homologs" } ] }